Coated nanoparticles enhance immune efficacy of Helicobacter pylori outer membrane vesicles by activating Th1/Th2/Th17 responses.

Antibiotic resistance has emerged as a pressing global public health crisis. The development of a safe and efficient Helicobacter pylori vaccine has become an important measure to eradicate Hpylori infection. Outer membrane vesicles (OMVs) secreted by Gram-negative bacteria are immunogenic and have received increasing attention in the development of vaccines against bacterial infections.

Occurrence of KPC-71 or the presence of NDM combined with a high expression of contributes to low-level cefiderocol resistance in carbapenem-resistant in China.

Low-level cefiderocol (CFDC) resistance in carbapenem-resistant (CRKP) represents a growing clinical concern due to its potential for rapid resistance escalation and silent dissemination under antibiotic pressure. This study investigated the molecular epidemiology and resistance mechanisms of low-level CFDC-resistant CRKP in eastern China. Among 382 CRKP clinical isolates, 29 (7.

Impact of fluoride varnish and oral health education on caries increment and dental plaque microbiota among children with early mixed dentition: a three-armed 24-month randomized controlled trial.

Dental caries is the most prevalent oral disease globally. A 24-month randomized controlled trial was conducted among 236 children aged between 7 and 8 years (ChiCTR2400084030). They were randomly allocated into three groups and received the following biannual interventions: Group 1: topical fluoride varnish (TFV) using 5% sodium fluoride; Group 2: oral health education (OHE); and Group 3: TFV + OHE.

Construction of dynamic ceRNA regulatory networks in osteogenesis during fracture healing based on transcriptomic analysis.

Fracture healing is a complex biological process. This study aimed to investigate the key molecules involved in fracture healing and their potential competing endogenous RNA (ceRNA) regulatory mechanisms within the first 28 days post-fracture using bioinformatics methods. The experiment was conducted on 15 adult male SD rats, with tibia callus tissue samples collected at days 0, 3, 7, 14, and 28 (n = 3) post-fracture.

Intestinal injury signaling pathway in sepsis.

Sepsis is a syndrome of inflammatory response syndrome (SIRS) triggered when the host is exposed to bacterial, viruses, and other infectious agents. The resulting inflammation compromises intestinal integrity, and this gut injury subsequently amplifies systemic inflammation, ultimately leading to multiple organ failure. This review synthesizes recent advances in sepsis-induced intestinal injury, focusing on four key aspects: pathogenesis, molecular mechanisms, crosstalk among relevant signaling pathways, and therapeutic strategies.

Bridging expectations and science: a roadmap for the future of longevity interventions.

The field of longevity interventions has witnessed rapid expansion, driven by scientific advancements alongside growing industry and consumer interest. However, no longevity intervention has yet been proven effective or ready for widespread clinical adoption. A substantial gap persists between public expectations and the current scientific realities.

Bimetallic Superlattice Enhances Efficiency and Stability of Top-Emitting Perovskite Light-Emitting Diodes.

Although perovskite emitters exhibit high electroluminescence quantum efficiency, the external quantum efficiency (EQE) of perovskite light-emitting diodes (PeLEDs) remains constrained by the refractive index contrast. This limitation is particularly pronounced in TE-PeLEDs, where severe optical losses arise from surface plasmon polarization (SPP) at the interfaces of the two metal electrodes. Here, we introduce a plasmonic scheme that leverages a bimetallic bottom electrode with a superlattice pattern to simultaneously enhance SPP outcoupling and introduce plasmon-enhanced fluorescence.

Deciphering the NLRP3 inflammasome in diabetic encephalopathy: Molecular insights and emerging therapeutic targets.

Diabetic encephalopathy (DE) is a neurological complication characterized by neuroinflammation, cognitive impairment, and memory decline, with its pathogenesis closely linked to the activation of the NLRP3 inflammasome. As a central regulator of the innate immune system, the NLRP3 inflammasome plays a pivotal role in DE progression by mediating neuroinflammation, pyroptosis, mitochondrial dysfunction, oxidative stress, endoplasmic reticulum (ER) stress, and microglial polarization. This review systematically explores the molecular mechanisms by which the NLRP3 inflammasome contributes to DE, focusing on its role in neuroinflammatory cascades and neuronal damage, as well as the diabetes-associated physiological changes that exacerbate DE pathogenesis.

Ultrafast micromixer for on-line screening of antioxidants.

Background: The integration of high-performance liquid chromatography (HPLC) with rapid post-column biochemical detection techniques has gained widespread application in the pinpoint of antioxidant components in complex natural matrices. However, the use of PEEK reaction coils in on-line antioxidant analysis often encounters issues with poor mixing of the reaction solution, leading to peak broadening and a decrease in peak capacity. This may adversely affect the sensitivity and precision of the analysis.

Adaptive Hardness-Driven Augmentation and Alignment Strategies for Multisource Domain Adaptations.

Multisource domain adaptation (MDA) aims to transfer knowledge from multiple labeled source domains to an unlabeled target domain. Nevertheless, traditional methods primarily focus on achieving interdomain alignment through sample-level constraints, such as maximum mean discrepancy (MMD), neglecting three pivotal aspects: 1) the potential of data augmentation; 2) the significance of intradomain alignment; and 3) the design of cluster-level constraints. In this article, we introduce a novel hardness-driven strategy for MDA tasks, named $\mathrm {A}^{3}\mathrm {MDA}$ , which collectively considers these three aspects through adaptive hardness quantification and utilization in both data augmentation and domain alignment.

Signaling networks in cancer stromal senescent cells establish malignant microenvironment.

The tumor microenvironment (TME) encompasses various cell types, blood and lymphatic vessels, and noncellular constituents like extracellular matrix (ECM) and cytokines. These intricate interactions between cellular and noncellular components contribute to the development of a malignant TME, such as immunosuppressive, desmoplastic, angiogenic conditions, and the formation of a niche for cancer stem cells, but there is limited understanding of the specific subtypes of stromal cells involved in this process. Here, we utilized p16-Cre-tdTomato mouse models to investigate the signaling networks established by senescent cancer stromal cells, contributing to the development of a malignant TME.

Targeting the HuR/E2F7 axis synergizes with bortezomib against multiple myeloma.

Multiple myeloma (MM) is a malignant hematological disease caused by the proliferation of abnormal plasma cells in the bone marrow and is still incurable. Relapse and drug resistance are common in MM. New therapeutic targets are urgently needed for MM treatment.

[Periodontic-orthodontic-orthognathic combined treatment of adult Class Ⅱ skeletal malocclusion with idiopathic gingival fibromatosis: a case report].

Idiopathic gingival fibromatosis is a rare, benign condition of unknown etiology characterized by extensive gingival overgrowth. This case reports a severe skeletal Class Ⅱ adult female patient with idiopathic gingival fibromatosis. The patient underwent multidisciplinary treatment involving periodontics, orthodontics, and orthognathic surgery, resulting in remarkable crown height elongation, substantial improvements in occlusal function and aesthetics, and stable long-term follow-up outcomes.

Bletilla striata polysaccharide induces autophagy through PI3K/AKT signaling pathway to promote the survival of cross-boundary flap in rats.

Introduction: Distal flap necrosis is a common problem in flap transplantation. Bletilla striata polysaccharide (BSP) is the main medicinal component of traditional Chinese medicine Bletilla striata. The purpose of this study was to investigate the mechanism of BSP promoting flap survival.

Synergistic effects of rhizosphere microbial communities and low molecular weight organic acids on Cd accumulation in Helianthus annuus L. in low-to-moderate cadmium-contaminated farmland.

Helianthus annuus L. (oil sunflower) is ideal candidate for phytoremediation of cadmium (Cd)-contaminated soil. To understand the relationship between the Cd accumulation in oil sunflower and its associated rhizosphere bacteria and root endophytic bacteria, this paper discussed the rhizosphere microecological characteristics and Cd accumulation of oil sunflower in various low-moderate Cd-contaminated farmlands.

Targeting the RBM39-MEK5 axis synergizes with bortezomib to inhibit the malignant growth of multiple myeloma.

Aberrant alternative splicing is one of the hallmarks of cancer and is potentially based on upregulated expression-of-splicing factors in some types of cancer. Our previous study suggested that the splicing factor RBM39 is significantly upregulated in multiple myeloma (MM) and that its upregulation is positively associated with poor prognosis. Here, we further demonstrate that the survival and proliferation of MM cells rely on RBM39 and that RBM39 knockdown inhibits the malignant growth of MM.

The complete mitochondrial genome of Gyrodactylus pseudorasborae (Platyhelminthes: Monogenea) with a phylogeny of Gyrodactylidae parasites.

Background: Gyrodactylus von Nordmann, 1832, a genus of viviparous parasites within the family Gyrodactylidae, contains one of the largest nominal species in the world. Gyrodactylus pseudorasborae Ondračková, Seifertová & Tkachenko, 2023 widely distributed in Europe and China, although its mitochondrial genome remains unclear. This study aims to sequence the mitogenome of G.

RAIN: Reconstructed-aware in-context enhancement with graph denoising for session-based recommendation.

Session-based recommendation aims to recommend the next item based on short-term interactions. Traditional session-based recommendation methods assume that all interacted items are closely related to the user's interests. However, noise (e.

Repairing alveolar bone defect using demineralized dentin grafts: a meta-analysis of randomized controlled trials.

Introduction: The alveolar bone defect often was hardly healed naturally by the body's repair mechanism. Implant placement was the most favored option for replacing a missing tooth in the current times, and the preservation of alveolar bone was vital. Tooth components, especially dentin, were very similar to alveolar bone components and could be used for promoting the alveolar bone regeneration process in reconstructive dentistry.

Natural compound phloretin restores periodontal immune homeostasis via HIF-1α-regulated PI3K/Akt and glycolysis in macrophages.

Periodontitis is a chronic inflammatory disease that affects about 45 %-50 % of adults worldwide, but the efficacy of current clinical therapies is unsatisfactory due to the complicated periodontal immune microenvironment. Thus, developing drugs that can regulate innate immune cells (e.g.

Nc-RNA-mediated low expression of AZIN1 correlated with unfavorable prognosis in kidney renal clear cell carcinoma.

Objective: Kidney renal clear cell carcinoma (KIRC, ccRCC) is the most common type of renal cancer with high recurrence and mortality. It has long been recognized that Antizyme inhibitor 1 (AZIN1) serves as a pro-oncogenic molecule in multiple cancers. However, the clinicopathological features of AZIN1 in KIRC remain unexplored.

1,3-Difunctionalization of Alkenes by Cobaloxime Photocatalysis.

Represented herein is the first 1,3-difunctionalization of alkenes via photocatalysis. A single cobaloxime is used to carry out two catalytic cycles in which cobaloxime is used not only as a photocatalyst to initiate the reaction but also as a metal catalyst for the β-H elimination process. Electron-deficient alkenes, electron-rich alkenes, and unactivated alkenes could be directly converted to 1,3-bisphosphorylated products, even unsymmetric 1,3-bisphosphorylated products, with only H as a byproduct under extremely mild reaction conditions.

Molecular Mechanisms Underlying Vascular Remodeling in Hypertension.

Hypertension, a common cardiovascular disease, is primarily characterized by vascular remodeling. Recent extensive research has led to significant progress in understanding its mechanisms. Traditionally, vascular remodeling has been described as a unidirectional process in which blood vessels undergo adaptive remodeling or maladaptive remodeling.