Functional potential and applications of Ureibacillus massiliensis based on whole-genome sequencing analysis.

Background: Ureibacillus massiliensis (U. massiliensis), a Gram-positive bacterium belonging to the phylum Bacillota, has undergone two significant taxonomic revisions before being classified under the Ureibacillus. Although previous studies have highlighted its promising applications in industrial production and environmental remediation, the lack of genomic information has limited the research on its functional mechanisms and industrial development.

Considering compound drought and heatwave events significantly improve forest pest occurrence prediction.

Background: The increasing frequency of compound drought and heatwave events (CDHWs) under global climate change has heightened the risk of forest pest outbreaks; however, precise quantitative assessments remain scarce. This study analyzed city-level forest pest incidence in China from 2003 to 2018 and utilized multiple machine learning models to quantify the impacts of droughts, heatwaves, and CDHWs on forest pest dynamics.

Results: The findings reveal that forest pest incidence exhibits a clear spatial pattern in China, with higher rates in the east and lower rates in the west, distinctly separated by the Hu Line.

Development and validation of nomograms for predicting survival of locally advanced rectosigmoid junction cancer patients: a SEER database analysis.

Background: Locally advanced rectosigmoid junction cancer (LARSJC) presents unique challenges in prognosis and treatment due to its anatomical ambiguity between the colon and rectum. Current staging systems may not sufficiently capture the heterogeneity of LARSJC, highlighting a clinical need for more accurate prognostic tools to guide treatment decisions and improve patient outcomes. This study aimed to investigate risk factors and develop nomograms for LARSJC patients to improve clinical outcomes across diverse patient subgroups.

Development and validation of survival prediction nomograms for patients with early-stage rectal cancer: a population-based study.

Background: The incidence of colorectal cancer (CRC) has been rising in recent years, with a concurrent increase in early-stage rectal cancer (ESRC) cases. This study aimed to investigate risk factors and developed nomograms to predict overall survival (OS) and cancer-specific survival (CSS) in ESRC patients in order to improve clinical outcomes across diverse patient subgroups.

Methods: Risk factors were investigated in ESRC patients by analyzing data from the Surveillance, Epidemiology, and End Results (SEER) database.

Development and validation of predictive nomograms for survival in early-onset colon cancer patients with II-III stage across various tumor sites.

Background: The incidence of colorectal cancer (CRC) has been escalating, with a concurrent rise in early-onset colon cancer (EOCC). Despite this alarming trend, the prognosis of EOCC has been understudied. Our study aims to identify risk factors associated with EOCC and develop nomograms for predicting overall survival (OS) and cancer-specific survival (CSS), with the goal of choosing suitable therapy for various patient subgroups.

Unraveling the genetic traits and functional diversity of the pan-genome in Pantoea dispersa.

Background: Medical devices are crucial in modern healthcare, but commonly used clinical tools such as cotton swabs can be easily contaminated by microorganisms (such as Pantoea), becoming vectors for pathogens and leading to patient infections or more severe outcomes. Despite the dual nature of the Pantoea that has garnered significant attention, research investigating Pantoea dispersa (P. dispersa) remains limited.

Development and validation of cancer-specific survival prediction nomogram for patients with T4 stage colon cancer after surgical resection: a population-based study.

Purpose: The increasing incidence of colorectal cancer has coincided with a rise in T4 stage colon cancer (CC), yet research on its prognosis remains limited. This study aimed to identify risk factors and develop a nomogram to predict cancer-specific survival (CSS), optimizing treatment strategies for different subgroups.

Methods: Using data from the from the Surveillance, Epidemiology, and End Results (SEER) database, we identified risk factors in T4 stage CC patients and created a nomogram to predict CSS.

DHS regulates leaf senescence by modulating gene translation in .

The biochemical and transcriptional regulatory mechanisms of chlorophyll metabolism have been extensively studied, but the translational regulatory mechanisms remain poorly understood. In this study, we found that Nt DHS1 deficiency in N. tabacum resulted in smaller leaves and increased leaf chlorophyll content.

Polar localization and local translation of RHO-RELATED PROTEIN FROM PLANTS2 mRNAs promote root hair growth in Arabidopsis.

Root hairs are tip-growing cells that anchor plants in the soil and are critical for water uptake, nutrient acquisition, and plant-environment interactions. While the molecular mechanisms that maintain the polar growth of root hairs through the asymmetric distribution of proteins, such as RHO-RELATED PROTEIN FROM PLANTS 2 (ROP2), have been described, it is unclear whether and how the transcripts encoding these tip-localized proteins are polarly localized and locally translated. Here, we demonstrated that ROP2 mRNA exhibits polar localization in Arabidopsis (Arabidopsis thaliana) root hairs.

Jasmonate induces translation of the Arabidopsis transfer RNA-binding protein YUELAO1, which activates MYC2 in jasmonate signaling.

Jasmonate is ubiquitous in the plant kingdom and regulates multiple physiological processes. Although jasmonate signaling has been thoroughly investigated in Arabidopsis thaliana, most studies have focused on the transcriptional mechanisms underlying various jasmonate responses. It remains unclear whether (and how) translation-related pathways help improve transcription efficiency to modulate jasmonate signaling, which may enable plants to respond to stressful conditions effectively.

Integrated respiratory toxicity of municipal wastewater to human bronchial epithelial cells and 3D bronchospheres.

Respiratory symptoms have been reported in wastewater treatment workers and residents living close to sewage treatment plant. However, toxicological research about the respiratory hazards of municipal wastewater is scarce. The present study aims to gain insight into the comprehensive respiratory hazards induced by the contaminant mixtures in municipal wastewater.

Fundus Tessellation and Parapapillary Atrophy, as Ocular Characteristics of Spontaneously High Myopia in Macaques: The Non-Human Primates Eye Study.

Purpose: This study aimed to evaluate the ocular characteristics associated with spontaneously high myopia in adult nonhuman primates (NHPs).

Methods: A total of 537 eyes of 277 macaques with an average age of 18.53 ± 3.

Single-cell transcriptomic Atlas of aging macaque ocular outflow tissues.

The progressive degradation in the trabecular meshwork (TM) is related to age-related ocular diseases like primary open-angle glaucoma. However, the molecular basis and biological significance of the aging process in TM have not been fully elucidated. Here, we established a dynamic single-cell transcriptomic landscape of aged macaque TM, wherein we classified the outflow tissue into 12 cell subtypes and identified mitochondrial dysfunction as a prominent feature of TM aging.

The FERONIA-YUELAO module participates in translational control by modulating the abundance of tRNA fragments in Arabidopsis.

tRNA fragments (tRFs) are a recently identified class of small noncoding RNAs. To date, the regulation of tRF abundance and its functional mechanisms have been largely unclear in plants. We investigated how the Arabidopsis thaliana receptor kinase FERONIA (FER) regulates the abundance of tRFs to inhibit global mRNA translation.

Development and validation of prognostic nomograms for early-onset colon cancer in different tumor locations: a population-based study.

Objective: The prevalence of early-onset colon cancer (EOCC) among individuals below the age of 50 has shown a marked upward trend in recent years. The embryology, clinical symptoms, incidence, molecular pathways, and oncologic outcomes differ between right-sided and left-sided colon cancers. However, the differences have not been fully researched in EOCC.

Numerical investigation of impinging jet ventilation in ICUs: Is thermal stratification a problem?

Intensive care units (ICUs) are the high incidence sites of hospital-acquired infections, where impinging jet ventilation (IJV) shows great potential. Thermal stratification of IJV and its effect on contaminants distribution were systematically investigated in this study. By changing the setting of heat source or the air change rates, the main driving force of supply airflow can be transformed between thermal buoyancy and inertial force, which can be quantitatively described by the dimensionless buoyant jet length scale ().

The functions of a 5' tRNA-Ala-derived fragment in gene expression.

Transfer RNA (tRNA) can produce smaller RNA fragments called tRNA-derived fragments (tRFs). tRFs play critical roles in multiple cellular programs, although the functional mechanisms of tRFs remain largely unknown in plants. In this study, we examined the phenotype associated with 5' tRF-Ala (tRF-Ala, produced from tRNA-Ala) overexpression and knockdown lines (tDR-Ala-OE and tDR-Ala-kd, respectively) and the mechanisms by which tRF-Ala affects mRNA levels in Arabidopsis (Arabidopsis thaliana).

Design, methodology, and preliminary results of the non-human primates eye study.

Purpose: To describe the normative profile of ophthalmic parameters in a healthy cynomolgus monkey colony, and to identify the characteristic of the spontaneous ocular disease non-human primates (NHP) models.

Methods: The NHP eye study was a cross-sectional on-site ocular examination with about 1,000 macaques held in Guangdong Province, southeastern China. The NHPs (Macaca fascicularis, cynomolgus) in this study included middle-aged individuals with a high prevalence of the ocular disease.

Cell surface receptor kinase FERONIA linked to nutrient sensor TORC signaling controls root hair growth at low temperature linked to low nitrate in Arabidopsis thaliana.

Root hairs (RH) are excellent model systems for studying cell size and polarity since they elongate several hundred-fold their original size. Their tip growth is determined both by intrinsic and environmental signals. Although nutrient availability and temperature are key factors for a sustained plant growth, the molecular mechanisms underlying their sensing and downstream signaling pathways remain unclear.

CAR modulates plasma membrane nano-organization and immune signaling downstream of RALF1-FERONIA signaling pathway.

In Arabidopsis, the receptor-like kinase (RLK) FERONIA (FER) senses peptide ligands in the plasma membrane (PM), modulates plant growth and development, and integrates biotic and abiotic stress signaling for downstream adaptive responses. However, the molecular interplay of these diverse processes is largely unknown. Here, we show that FER, the receptor of Rapid Alkalinization Factor 1 (RALF1), physically interacts with C2 domain ABA-related (CAR) proteins to control the nano-organization of the PM.

The rubber tree RALF peptide hormone and its receptor protein kinase FER implicates in rubber production.

RAPID ALKALINIZATION FACTORs (RALFs), which are secreted peptides serving as extracellular signals transduced to the inside of the cell, interact with the receptor-like kinase FERONIA (FER) and participates in various biological pathways. Here, we identified 23 RALF and 2 FER genes in Hevea brasiliensis (para rubber tree), and characterized their expression patterns in different tissues, across the process of leaf development, and in response to the rubber yield-stimulating treatments of tapping and ethylene. Four Hevea latex (the cytoplasm of rubber-producing laticifers)-abundant RALF isoforms, HbRALF19, HbRALF3, HbRALF22, and HbRALF16 were listed with descending expression levels.

FERONIA is involved in phototropin 1-mediated blue light phototropic growth in Arabidopsis.

Plant shoot phototropism is triggered by the formation of a light-driven auxin gradient leading to bending growth. The blue light receptor phototropin 1 (phot1) senses light direction, but how this leads to auxin gradient formation and growth regulation remains poorly understood. Previous studies have suggested phot1's role for regulated apoplastic acidification, but its relation to phototropin and hypocotyl phototropism is unclear.