Cu-Fe bimetallic peroxide-based nanozyme with microenvironment-triggered cascade catalysis for synergistic hydroxyl radical, nitric oxide, and oxygen generation in trimodal wound infection therapy.

Recently, chemodynamic therapy (CDT) and gas therapy (GT) have garnered significant attention for antibacterial applications. However, effectively integrating CDT and GT into a single nanocomposite for synergistic therapy remains challenging. Herein, Cu-Fe bimetallic peroxide nanoparticles (CFp)-decorated hollow polydopamine (HPDA) nanozyme (CFp/HPDA) was fabricated and used as the carrier for nitric oxide (NO) donor BNN6 loading, resulting in the NO-releasing nanozyme of CFp/HPDA@BNN6.

Integrating computational pathology and multi-transcriptomics to characterize lung adenocarcinoma heterogeneity and prognostic modeling.

Background: Lung adenocarcinoma (LUAD) is the most prevalent subtype of non-small cell lung cancer (NSCLC), characterized by high molecular and pathological heterogeneity. While traditional histopathology plays a key role in LUAD diagnosis, integrating computational pathology with multi-omics analysis provides novel insights into tumor microenvironment (TME) dynamics and molecular mechanisms. However, the relationship between pathological histological features and genomic instability in LUAD remains poorly understood.

Cross Talk Between Macrophages and Podocytes in Diabetic Nephropathy: Potential Mechanisms and Novel Therapeutics.

Diabetic nephropathy (DN) is a leading cause of chronic kidney disease and end-stage renal failure worldwide. Podocytes, essential components of the glomerular filtration barrier (GFB), are profoundly affected in the diabetic milieu, resulting in structural and functional alterations. Concurrently, macrophages, pivotal innate immune cells, infiltrate the diabetic kidney and exhibit diverse activation states influenced by the local environment, playing a crucial role in kidney physiology and pathology.

Monodisperse Silica Microsphere with Extremely Large Specific Surface Area: Preparation and Characterization.

Monodisperse SiO microspheres are widely used in catalysis, separation, adsorption, and drug delivery. Their particle size, uniformity, and specific surface area are crucial for these applications. This study reports the novel preparation of monodisperse SiO microspheres using cetyltrimethylammonium bromide as the template agent, employing hexadecylamine serving concurrently as a pore-expanding agent and catalyst.

Near Infrared II Laser-Boosted Peroxynitrite Generation Hollow Copper Single Atom Nanozyme for Effective Bacterial Infection Treatment.

The peroxynitrite anion (ONOO) is the product of a specific reaction between nitric oxide (NO) and the superoxide anion (O ), showing great potential for treating multidrug-resistant bacterial infections. However, antibacterial materials with highly efficient ONOO controlled release properties are lacking. In this study, hollow copper single atom nanozyme (Cu HSAz) with oxidase (OXD)-mimicking property is rationally designed and used for the first time as effective delivery carrier for the NO donor N, N'-di-sec-butyl-N, N'-dinitroso-1, 4-phenylenediamine (BNN6), obtaining the nanozyme Cu HSAz@BNN6.

Current Advances and Future Prospects of Bulk and Microfluidic-Enabled Electroporation Systems.

Reversible electroporation (EP) is a pivotal biophysical technology that leverages pulsed electric fields to enhance the permeability of cell membranes, thereby facilitating the introduction of foreign material into cells. In this review, we provide an overview of bulk electroporators and microfluidic-enabled EP systems, focusing on their controversial points of mechanisms, architectures, and parameter settings. Bulk electroporators have been extensively commercialized with settled form including pulse generator and accessories (i.

Hybrid membrane based biomimetic nanodrug with high-efficient melanoma-homing and NIR-II laser-amplified peroxynitrite boost properties for enhancing antitumor therapy via effective immunoactivation.

Owing to the excellent stability, anticancer activity and immunogenicity, peroxynitrite (ONOO) has been gained enormous interests in cancer therapy. Nevertheless, precise delivery and control release of ONOO in tumors remains a big challenge. Herein, B16F10 cancer cell membrane/liposome hybrid membrane (CM-Lip) based biomimetic nanodrug with high-efficient tumor-homing and NIR-II laser controlled ONOO boost properties was designed for melanoma treatment.

The immune regulatory role of exosomal miRNAs and their clinical application potential in heart failure.

Heart failure (HF) is a complex and debilitating condition characterized by the heart's inability to pump blood effectively, leading to significant morbidity and mortality. The abnormality of immune response is a key factor in the progression of HF, contributing to adverse cardiac remodeling and dysfunction. Exosomal microRNAs (miRNAs) play a pivotal role in regulating gene expression and cellular function, which are integral to the crosstalk between cardiac and immune cells, influencing immune cell functions, such as macrophage polarization, T cell activity, and cytokine production, thereby modulating various pathological processes of HF, such as inflammation, fibrosis, and cardiac dysfunction.

The Antipsychotic Drug Aripiprazole Suppresses Colorectal Cancer by Targeting LAMP2a to Induce RNH1/miR-99a/mTOR-Mediated Autophagy and Apoptosis.

The mammalian target of rapamycin (mTOR) is a critical signaling hub for sustaining cancer survival. Targeting mTOR and inducing autophagic cell death downstream of it represent promising therapeutic strategies for cancer prevention. A US Food and Drug Administration-approved drug library containing 616 small molecules is used to screen anticancer drugs against colorectal cancer (CRC) cells that rely on mTOR.

The mA-ncRNAs axis in diabetes complications: novel mechanism and therapeutic potential.

Diabetes, a multifaceted metabolic disorder, poses a significant global health burden with its increasing prevalence and associated complications, such as diabetic nephropathy, diabetic retinopathy, diabetic cardiomyopathy, and diabetic angiopathy. Recent studies have highlighted the intricate interplay between N-methyladenosine (mA) and non-coding RNAs (ncRNAs) in key pathways implicated in these diabetes complications, like cell apoptosis, oxidative stress, and inflammation. Thus, understanding the mechanistic insights into how mA dysregulation impacts the expression and function of ncRNAs opens new avenues for therapeutic interventions targeting the mA-ncRNAs axis in diabetes complications.

CD-MOF-1 Growth on Polysaccharide Gels through Only C2-OH/C3-OH or C5-O/C6-OH Group Formed Four-Coordinated K Ions for Developing Porous Biogels.

The γ-cyclodextrin (γ-CD) metal-organic frameworks (CD-MOF-1) consist of γ-CD and potassium (K) ions through coordinating an eight-coordinated K ion with two C5-linked oxygen and C6-linked hydroxyl (C5-O/C6-OH) groups in the primary faces of adjacent γ-CD units and two C2- and C3-linked hydroxyl (C2-OH/C3-OH) groups in the secondary faces. Herein, we found polysaccharide gels with only C2-OH/C3-OH or C5-O/C6-OH groups in pyranoid rings can form four-coordinated K ions and then coordinate γ-CD in a KOH solution for CD-MOF-1 growth. Exposure of C2-OH/C3-OH or C5-O/C6-OH groups in polysaccharide gels is important to form active four-coordinated K ions.

Copper peroxide nanodot-decorated gold nanostar/silica nanorod Janus nanostructure with NIR-II photothermal and acid-triggered hydroxyl radical generation properties for the effective treatment of wound infections.

Recently, bacterial infections have become a global crisis, greatly threatening the health of human beings. The development of a non-antibiotic biomaterial is recognized as an alternative way for the effective treatment of bacterial infections. In the present work, a multifunctional copper peroxide (CP) nanodot-decorated gold nanostar (GNS)/silica nanorod (SiNR) Janus nanostructure (GNS@CP/SiNR) with excellent antibacterial activity was reported.

The regulatory role of miRNA and lncRNA on autophagy in diabetic nephropathy.

Diabetic nephropathy (DN) is a serious complication of diabetes that causes glomerular sclerosis and end-stage renal disease, leading to ascending morbidity and mortality in diabetic patients. Excessive accumulation of aberrantly modified proteins or damaged organelles, such as advanced glycation end-products, dysfunctional mitochondria, and inflammasomes is associated with the pathogenesis of DN. As one of the main degradation pathways, autophagy recycles toxic substances to maintain cellular homeostasis and autophagy dysregulation plays a crucial role in DN progression.

Utilizing machine learning algorithms to identify biomarkers associated with diabetic nephropathy: A review.

Diabetic nephropathy (DN), a multifaceted disease with various contributing factors, presents challenges in understanding its underlying causes. Uncovering biomarkers linked to this condition can shed light on its pathogenesis and support the creation of new diagnostic and treatment methods. Gene expression data were sourced from accessible public databases, and Weighted Gene Co-expression Network Analysis (WGCNA)was employed to pinpoint gene co-expression modules relevant to DN.

Anticancer effect of hUC-MSC-derived exosome-mediated delivery of PMO-miR-146b-5p in colorectal cancer.

Antisense oligonucleotide (ASO) is a novel therapeutic platform for targeted cancer therapy. Previously, we have demonstrated that miR-146b-5p plays an important role in colorectal cancer progression. However, a safe and effective strategy for delivery of an ASO to its targeted RNA remains as a major hurdle in translational advances.

Revealing the antibacterial power of hydrogen-releasing PdH nanohydride against drug resistant : an in-depth mechanism study.

Currently, multidrug resistant (MDR) bacterial infections are a great threat to public health, and the development of novel strategies for high efficiency combatting of MDR bacteria is in urgent demand. Hydrogen (H) is a small gas with a high reducing ability, and plenty of recent studies have demonstrated its therapeutic effect on many diseases. However, the antibacterial effectiveness and mechanism of H against MDR bacteria are still unknown.

Near-infrared laser-controlled nitric oxide-releasing gold nanostar/hollow polydopamine Janus nanoparticles for synergistic elimination of methicillin-resistant Staphylococcus aureus and wound healing.

Recently, nitric oxide (NO) has received increasing interest in combat against bacteria-induced infections because of its ability to sensitize and enhance the antibacterial effectiveness of many therapeutic approaches such as antibiotics. However, high-efficient loading and controlled release of NO remain a big challenge. In the present work, a type of gold nanostar/hollow polydopamine Janus nanostructure (GNS/HPDA JNPs) with precise near infrared (NIR)-controlled NO release property was fabricated using a facile seed-mediated method.

Hierarchically Hybrid Porous CoO@NiMoO/CoMoO Heterostructures for High-Performance Electrochemical Energy Storage.

Hierarchical, ultrathin, and porous NiMoO@CoMoO on CoO hollow bones were successfully designed and synthesized by a hydrothermal route from the Co-precursor, followed by a KOH (potassium hydroxide) activation process. The hydrothermally synthesized CoO nanowires act as the scaffold for anchoring the NiMoO@CoMoO units but also show more compatibility with NiMoO, leading to high conductivity in the heterojunction. The intriguing morphological features endow the hierarchical CoO@NiMoO@CoMoO better electrochemical performance where the capacity of the CoO@NiMoO@CoMoO heterojunction being 272 mA·h·g at 1 A·g can be achieved with a superior retention of 84.

Biodegradable Hollow Polydopamine@manganese Dioxide as an Oxygen Self-Supplied Nanoplatform for Boosting Chemo-photodynamic Cancer Therapy.

Photodynamic therapy (PDT) has attracted extensive attention in the clinical treatment of malignant tumor. However, the acidic and hypoxic conditions of the tumor microenvironment (TME) limit the further application of PDT in the clinic. Herein, we fabricate a new nanoplatform─HPDA@MnO@Ce6/DOX@PEG-RGD (HPMRCD)─by means of coating hollow polydopamine nanoparticles (HPDA) with manganese dioxide (MnO), which is modified by cyclic RGD functionalized poly(ethylene glycol) (PEG) and further co-loaded with a photosensitizer, Chlorin e6 (Ce6), and a chemotherapy drug, doxorubicin (DOX).

Near infrared II laser controlled free radical releasing nanogenerator for synergistic nitric oxide and alkyl radical therapy of breast cancer.

Recently, alkyl radicals have attracted much attention in cancer therapy due to their oxygen-independent generation property. For the first time, alkyl radical and nitric oxide (NO) combined therapy is demonstrated as an effective strategy for tumor inhibition. As a proof of concept, a biocompatible free radical nanogenerator with near-infrared (NIR) II laser-induced simultaneous NO and alkyl radical release property was elaborately fabricated.

A Panel of Plasma Exosomal miRNAs as Potential Biomarkers for Differential Diagnosis of Thyroid Nodules.

A liquid biopsy using circulating exosomal genetic materials provides new insights for thyroid cancer diagnosis. This study aimed to identify plasma-derived exosomal biomarkers that could be used for early detection of papillary thyroid carcinoma (PTC). Exosomal miRNAs in plasma were isolated from patients with benign thyroid nodules and patients with PTC.

The versatile roles and clinical implications of exosomal mRNAs and microRNAs in cancer.

Extracellular vesicles (EVs), which include exosomes, microvesicles, and apoptotic bodies, are nanosized structures that are secreted by various cells and act as important mediators in intercellular communication. Recent studies have shown that exosomes carrying bioactive molecules are generated from multivesicular bodies and are present in various body fluids. mRNAs and microRNAs (miRNAs) are encapsulated in exosomes and have been found to be involved in multiple pathophysiological processes.