Investigating the Presence and Genetic Variability of Porcine Circovirus Types 2 and 3 in Live Markets in Border Cities of Northeast China.

Bordering cities and farms serve as potential hotspots for the transboundary spread of animal infectious agents. This study aimed to investigate the presence and genetic variability of porcine circovirus types 2 (PCV2) and 3 (PCV3) in live markets across six border cities (Dandong, Ji'an, Hunchun, Mishan, Fuyuan, and Heihe) in northeast China. Samples from pork ( = 44), cutting boards ( = 46), and meat stall floors ( = 42) were collected in 46 meat stalls.

[Biomechanical analysis of a novel bridging plate for treating Rockwood III acromioclavicular joint dislocation].

Objectives: To assess the biomechanical performance of a novel bridging plate for treating Rockwood III acromioclavicular joint dislocation.

Methods: A novel bridging plate structure was designed based on CT data from a patient with Rockwood type III acromioclavicular joint dislocation, and a finite element model of the bridging plate-acromioclavicular joint interaction was constructed. The stress and deformation characteristics and biomechanical compatibility of the plate under post-reduction, normal loading, and impact loading conditions were analyzed to evaluate its fixation mechanism and clinical advantages.

Eradication of Helicobacter pylori that contributes to hepatogenic ulcer is beneficial to the healing of hepatogenic ulcer.

Background: This study aimed to explore the role of Helicobacter pylori (Hp) eradication in patients with hepatogenic ulcer (HU).

Methods: Patients with HU, patients with liver cirrhosis without peptic ulcer and patients with functional dyspepsia (FD) were selected and the relationship between complications of liver cirrhosis and Hp infection in HU patients was evaluated retrospectively. Furthermore, 60 HU patients with Hp infection were randomly divided into the treatment group with rabeprazole plus amoxicillin and levofloxacin, and the control group with rabeprazole alone and the therapeutic effects then were recorded.

Emergence, prevalence and evolution of porcine reproductive and respiratory syndrome virus 1 in China from 1994 to 2024.

Porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) was first detected in Chinese swine herds during an epidemiological investigation since 1994. Even though PRRSV-1 has been existed in China for 30 years, much less attention was paid on PRRSV-1 than PRRSV-2. This review systematically evaluated the emergence, prevalence and evolution of Chinese PRRSV-1 from 1994 to 2024.

Mapping risks: A value chain approach to brucellosis introduction in Zhijiang's Cattle Population, China.

Brucellosis, a zoonotic disease primarily transmitted through livestock, poses significant health and economic challenges globally. Its increasing incidence in China highlights critical gaps in current control measures, particularly in regions with high livestock movement, where domestic ruminants trade networks elevate the risk of disease transmission. Traditional value chain analyses often provide descriptive mappings of these networks but lack quantitative methods to assess specific risk levels.

The efficacy of sequentially comprehensive treatment based on surgery in the treatment of keloids: a retrospective study.

Purpose: The objective of this study is to investigate the clinical efficacy of sequentially comprehensive treatment based on surgery and to furnish clinical evidence for the management of keloids.

Patients And Methods: The patients with keloids were retrospectively analyzed who underwent surgery-based sequentially comprehensive treatment at the Plastic Surgery Department of Shandong Provincial Hospital from January 2018 to August 2021. The recurrence rate and incidence of adverse reactions were explored for all the included patients.

Suboptimal distributed cooperative control for linear multi-agent system via Riccati design.

In this paper, we propose a suboptimal distributed cooperative control scheme for the continuous-time linear multi-agent system (MAS) with a specified global quadratic cost functional over both undirected and directed graph scenarios. For undirected graphs, we first derive the cost functional for a given strictly linear feedback distributed protocol. It is shown that the cost functional is upper bounded by a quadratic form of the MAS's initial state, and the minimum upper bound can be derived by solving a parametric algebraic Riccati equation (PARE) depends solely on the algebraic connectivity of the graph and is independent of the largest eigenvalue compared with the existing work.

Revealing the formation mechanisms of key flavor components during the fermentation of bamboo shoots by combining flavoromics and metagenomics.

Microbial metabolism plays a critical role in the flavor development of Guangxi fermented bamboo shoots (GFBS). To clarify the role of microorganisms in flavor formation and predict the metabolic pathways of key characteristic flavor compounds, this study employed metabolomics, Odor Activity Value (OAV), and Taste Activity Value (TAV) calculations, integrated with Partial Least Squares Discriminant Analysis (PLS-DA), to investigate changes in GFBS flavors-represented by volatile flavor compounds, organic acids, and free amino acids-across a 30-day fermentation period. Metagenomic datasets were used to identify taxonomic and functional changes in the microbial community.

A Review of the Nutritional Composition, Storage Challenges, Processing Technology and Widespread Use of Bamboo Shoots.

Bamboo shoots, as the young bamboo stems, are rich in protein, fiber, vitamins, and minerals, as well as many bioactive substances beneficial to health, and are gaining in importance worldwide as a healthy food and dietary supplement. However, fresh bamboo shoots lignify rapidly after harvesting and contain cyanogenic glycosides, limiting the safe and healthy consumption of bamboo shoots. To this end, based on the changes in nutritional composition and the physiological properties of fresh and post-harvest bamboo shoots, factors affecting the preservation of post-harvest bamboo shoots are emphasized, including a series of physical and chemical regimes and various processing methods for post-harvest preservation.

Purification, characterization, and hypoglycemic activity of exopolysaccharides from Lactiplantibacillus plantarum MY04.

As natural polymers, Lactiplantibacillus plantarum exopolysaccharides (EPS) possess a wide range of bioactivities but suffer from low yields and unclear relationships between functional activity and structure. To this end, the chemical structure and bioactive properties of EPS from Lactiplantibacillus plantarum MY04 were investigated. As a result, three polysaccharide fractions (EPS-1, EPS-2, EPS-3) were separated and purified, of which EPS-2 had better antioxidant activity and α-glucosidase inhibitory ability.

Fed-batch fermentation strategy for efficient welan gum production by Sphingomonas sp. FM01.

Background: As a novel type of extracellular polysaccharide produced by Sphingomonas sp., welan gum has been widely applied in various fields because of its excellent properties. The study has improved the fermentation process.

Assessment of the Safety and Exopolysaccharide Synthesis Capabilities of Bacillus amyloliquefaciens D189 Based on Complete Genome and Phenotype Analysis.

Exopolysaccharides (EPS) are natural macromolecular carbohydrates with good functional activity and physiological activities, which can be utilized as an emulsifier, viscosity enhancer, stabilizer, gelling agent, and water retention agent in a wide range of food products. In this study, the whole genome of Bacillus amyloliquefaciens D189, an EPS-producing bacteria, was sequenced. The result showed that D189 contains a single, circular chromosome of 3,963,356 bp with an average GC content of 45.

Screening of the Lipid-Lowering Probiotic Lactiplantibacillus Plantarum SDJ09 and its Anti-Obesity Mechanism.

In this study, 39 strains of lactic acid bacteria were screened from several fermented foods. Based on the evaluation of functional and prebiotic properties, Lactiplantibacillus plantarum SDJ09 was selected as a promising candidate. It gave a 48.

Presence of Epstein-Barr virus in cerebrospinal fluid is associated with increased mortality in HIV-negative cryptococcal meningitis.

Cryptococcus neoformans is the most common cause of fungal meningitis and is associated with a high mortality. The clinical significance of concurrent Epstein-Barr virus (EBV) in the cerebrospinal fluid (CSF) of human immunodeficiency virus (HIV)-negative patients with cryptococcal meningitis (CM) remains unclear. A retrospective cohort study was performed by analyzing CSF samples from 79 HIV-negative Chinese Han patients with confirmed CM.

Developmental relationship between junctional epithelium and epithelial rests of Malassez.

(K17) is thought to be a candidate target gene for regulation by Lymphoid Enhancer Factor-1 (Lef-1) K17 is a marker that distinguishes junctional epithelium (JE) from epithelial rests of Malassez (ERM). However, the relationship of Lef-1 to K17 is not clear in this context. Moreover, the expression of other keratins such as K5, K6, K7 and K16 is not reported.

Xylitol fermentation characteristics with a newly isolated yeast Wickerhamomyces anomalus WA.

Xylitol is an increasingly popular functional food additive, and the newly isolated yeast Wickerhamomyces anomalus WA has shown extensive substrate utilization capability, with the ability to grow on hexose (d-galactose, d-glucose, d-mannose, l-fructose, and d-sorbose) and pentose (d-xylose and l-arabinose) substrates, as well as high tolerance to xylose at concentrations of up to 300 g/L. Optimal xylitol fermentation conditions were achieved at 32 °C, 140 rpm, pH 5.0, and initial cell concentration OD of 2.

Protects the Intestinal Epithelium and Mitigates Inflammation in Colitis via Regulating the Gut Microbiota-Cholic Acid Pathway.

In this study, we aimed to explore the protective effects of in colitis mice and the potential mechanisms. Results showed that () effectively colonized the intestinal tract and alleviated colitis symptoms by reducing the disease activity index. Moreover, mitigated intestinal epithelial cell damage, inhibited the pro-inflammatory factors, and upregulated tight junction (TJ)-proteins.

Isolation, identification, and preparation of tyrosinase inhibitory peptides from Pinctada martensii meat.

Recently, natural tyrosinase inhibitors have gained attention in clinical cosmetology research. In this study, the enzymatic hydrolysis of Pinctada martensii meat by protease from Bacillus licheniformis, 401 peptides with tyrosinase inhibitory were identified after isolated by ultrafiltration and Sephadex G-15 from the fraction F4. The peptide effects on the tyrosinase activity and structure were evaluated using molecular docking.

Non-metabolic enzyme function of PKM2 in hepatocellular carcinoma: A review.

Hepatocellular carcinoma (HCC) is one of the most malignant tumors with the highest incidence and mortality in the world, causing a serious burden on society. Pyruvate kinase M2 (PKM2) is one of the principal metabolic enzymes involved in glycolysis. Studies have shown that PKM2 is highly expressed in HCC and can be translocated to the nucleus, where it interacts with various transcription factors and proteins such as hypoxia-inducible factor-1α, sterol regulatory element-binding protein 1a, signal transducer and activator of transcription 3, nuclear factor erythroid 2-like 2 and histone H3, exerting non-metabolic enzyme functions to regulate the cell cycle, proliferation, apoptosis, immune escape, migration, and invasion, as well as HCC angiogenesis and tumor microenvironment.

Structural insights of a SusD-like protein in marine Bacteroidetes bacteria reveal the molecular basis for chitin recognition and acquisition.

Efficient recognition and transportation of chitin oligosaccharides are crucial steps for the utilization of chitin by heterotrophic bacteria. In this study, we employed structural biological and biochemical approaches to investigate the substrate recognition and acquisition mechanism of a novel chitin-binding SusD-like protein, AqSusD, which is derived from the chitin utilization gene cluster of a marine Bacteroides strain (Aquimarina sp. SCSIO 21287).

In-Situ Study on the Tensile Deformation and Fracture Mechanism of a Bimodal-Structured Mg-Gd-Y Alloy.

The as-extruded (EX) Mg-Gd-Y alloy studied here exhibited a bimodal structure, composed of fine dynamic recrystallized (DRXed) grains with random orientations and longitudinal coarse hot-worked grains. The slip analysis showed the DRXed grains exhibited mainly basal slips, while the hot-worked grains exhibited mainly prismatic slips during the tensile deformation. The distribution of geometrically necessary dislocations (GNDs) showed that there was strain partitioning between the fine and coarse grain regions.

PSME3 induces radioresistance and enhances aerobic glycolysis in cervical cancer by regulating PARP1.

Cervical cancer (CC) ranks the fourth in gynecologic cancers. The incidence and mortality of CC has been decreased due to the cancer screening and early treatments in recent years, but the prognosis of CC patients at advanced stage is still sorrowful. Whether PSME3 exerted a role in the radioresistance of CC cells remains to be investigated.

Maslinic acid alleviates LPS-induced mice mastitis by inhibiting inflammatory response, maintaining the integrity of the blood-milk barrier and regulating intestinal flora.

Mastitis occurs frequently in breastfeeding women and not only affects the women's health but also hinders breastfeeding. Maslinic acid is a type of pentacyclic triterpenoid widely found in olives that has good anti-inflammatory activity. This study aims to discuss the protective function of maslinic acid against mastitis and its underlying mechanism.

Melatonin regulates cancer migration and stemness and enhances the anti-tumour effect of cisplatin.

Melatonin, a lipophilic hormone released from the pineal gland, has oncostatic effects on various types of cancers. However, its cancer treatment potential needs to be improved by deciphering its corresponding mechanisms of action and optimising therapeutic strategy. In the present study, melatonin inhibited gastric cancer cell migration and soft agar colony formation.