Effect of COVID-19 inactivated vaccine on anti-Müllerian hormone in Chinese women: a retrospective cohort study.

Introduction: This study aimed to assess the impact of inactivated COVID-19 vaccine on Anti-Müllerian hormone (AMH) levels in Chinese women.

Methods: A retrospective analysis was conducted on women aged 18-45 who had undergone two AMH tests between March 2020 and September 2021. Participants were grouped based on vaccine doses (two- and three-dose), time intervals since vaccination, and manufacturers.

Spatial and Temporal Distribution of White Matter Lesions in NOTCH2NLC-Related Neuronal Intranuclear Inclusion Disease.

Background And Objectives: -related neuronal intranuclear inclusion disease (NIID) is a neurodegenerative disease with characteristic white matter lesions (WMLs) visible on MRI. However, the distribution of WMLs and their clinical correlations remain poorly understood in NIID. This study aims to investigate the spatial and temporal distribution of WMLs in the brain of patients with -related NIID.

Microglia contribute to polyG-dependent neurodegeneration in neuronal intranuclear inclusion disease.

Neuronal intranuclear inclusion disease (NIID) is a neurodegenerative disorder caused by the expansion of GGC trinucleotide repeats in NOTCH2NLC gene. Despite identifying uN2CpolyG, a toxic polyglycine (polyG) protein translated by expanded GGC repeats, the exact pathogenic mechanisms of NIID remain unclear. In this study, we investigated the role of polyG by expressing various forms of NOTCH2NLC in mice: the wild-type, the expanded form with 100 GGC repeats (either translating or not translating into uN2CpolyG), and the mutated form that encodes a pure polyG without GGC-repeat RNA and the C-terminal stretch (uN2CpolyG-dCT).

Reversible encephalitis-like episodes in fragile X-associated tremor/ataxia syndrome: a case report.

Background: Fragile X-associated tremor/ataxia syndrome (FXTAS) is a neurodegenerative disorder caused by CGG repeat expansion of FMR1 gene. Both FXTAS and neuronal intranuclear inclusion disease (NIID) belong to polyglycine diseases and present similar clinical, radiological, and pathological features, making it difficult to distinguish these diseases. Reversible encephalitis-like attacks are often observed in NIID.

Heterozygous RELN missense variants associated with genetic generalized epilepsy.

Purpose: The RELN gene encodes the secreted glycoprotein Reelin and has important functions in both developing and adult brains. In this study, we aimed to explore the association between the RELN and genetic generalized epilepsy (GGE).

Methods: We performed whole-exome sequencing on a cohort of 92 patients with GGE.

White matter integrity mediates the associations between white matter hyperintensities and cognitive function in patients with silent cerebrovascular diseases.

Objective: To evaluate the relationships between cognitive function and white matter hyperintensity volume (WMHV) in patients with silent cerebrovascular disease and to investigate whether white matter integrity or brain atrophy play a role in this association.

Methods: Automated Fiber Quantification and Voxel- based morphometry were used to track and identify the integrity of 20 well-defined white matter tracts and to measure the gray matter volume (GMV). A linear regression model was applied for examining the associations between cognitive function and WMHV and mediation analysis was used to identify the roles of white matter integrity or GMV in the influence of WMHV on cognitive function.

Integration analysis identifies the role of metallothionein in the progression from hepatic steatosis to steatohepatitis.

Background: Non-alcoholic fatty liver disease (NAFLD), a metabolic disorder that develops from non-alcoholic fatty liver (NAFL) to non-alcoholic steatohepatitis (NASH), has become an epidemic of chronic liver dysfunction worldwide. However, mechanisms that govern the transition from NAFL to NASH have not been fully elucidated.

Methods: Gene expression profile data of NAFLD liver tissues were obtained from Gene Expression Omnibus (GEO), including three microarray datasets with 60 NAFL and 44 NASH patients.

Upstream open reading frame with NOTCH2NLC GGC expansion generates polyglycine aggregates and disrupts nucleocytoplasmic transport: implications for polyglycine diseases.

Neuronal intranuclear inclusion disease (NIID) is neurodegenerative disease characterized by widespread inclusions. Despite the identification of GGC repeat expansion in 5'UTR of NOTCH2NLC gene in adult-onset NIIDs, its pathogenic mechanism remains unclear. Gain-of-function poly-amino-acid proteins generated by unconventional translation have been revealed in nucleotide repeat expansion disorders, inspiring us to explore the possibility of unconventional translation in NIID.

Bioinformatic analysis identifies key transcriptome signatures in temporal lobe epilepsy.

Aims: To identify transcriptome signatures underlying epileptogenesis in temporal lobe epilepsy (TLE).

Methods: Robust rank aggregation analysis was used to integrate multiple microarrays in rodent models of TLE and determine differentially expressed genes (DEGs) in acute, latent, and chronic stages. Functional annotation and protein-protein interaction analysis were performed to explore the potential functions of the DEGs and identify hub genes with the highest intramodular connectivity.

GABAergic Interneuron and Neurotransmission Are mTOR-Dependently Disturbed in Experimental Focal Cortical Dysplasia.

Focal cortical dysplasia (FCD) is a major cause for drug-resistant epilepsies. The molecular and cellular mechanisms of epileptogenesis in FCD are still poorly understood. Some studies have suggested that deficiencies of γ-aminobutyric acid (GABA) system may play an important role in type II FCD, but it remains controversial.

The Rodent Models of Dyskinesia and Their Behavioral Assessment.

Dyskinesia, a major motor complication resulting from dopamine replacement treatment, manifests as involuntary hyperkinetic or dystonic movements. This condition poses a challenge to the treatment of Parkinson's disease. So far, several behavioral models based on rodent with dyskinesia have been established.

[Prenatal diagnosis of a novel SOX10 mutation in a patient with syndromic hearing loss].

Objective: To explore the genetic basis for a patient with syndromic hearing loss.

Methods: Genomic DNA of the patient was extracted, for which 127 deafness-related genes were enriched with a chip. Following next generation sequencing, pathogenic loci in exonic regions were analyzed through comparison against the databases.

Effects of fiber alignment on stem cells-fibrous scaffold interactions.

Considerable research has been focused on the utilization of electrospun ultrafine fibers to construct biomimetic scaffolds for tissue engineering applications. However, there is still a limited understanding of the effects of fiber alignment on stem cells-fibrous scaffold interactions and their role in deciding the fate of stem cells and scaffolds. Here, we tracked the real-time interactions between mesenchymal stem cells (MSCs) and aligned/non-aligned electrospun PCL-gelatin ultrafine fibers using time-lapse microscopy, and investigated the effects of fiber alignment on the behaviour of cell spreading, migration and differentiation, and scaffold remodeling.

Effect of ultrasound on cyprid footprint and juvenile barnacle adhesion on a fouling release material.

In our earlier studies, we have demonstrated that low and high intensity ultrasound can prevent barnacle cyprid settlement. In this study, we found that ultrasound treatment reduced the adhesion of newly metamorphosed barnacles up to 2 days' old. This was observed in the reduction of adhesion strength of the newly settled barnacles from ultrasound treated cyprids on silicone substrate compared to the adhesion strength of barnacles metamorphosed from cyprids not exposed to ultrasound.

Adhesion of B. subtilis spores and vegetative cells onto stainless steel--DLVO theories and AFM spectroscopy.

Interactions between the bacterium Bacillus subtilis (either as vegetative cells or as spores) and stainless steel 316 (SS-316) surfaces were quantified using the classical Derjaguin-Landau-Verwey-Overbeek (DLVO) theory and extended DLVO (xDLVO) approach in conjunction with live force spectroscopy using an Atomic Force Microscope (AFM). The xDLVO approach accounts for acid-base (polar) interactions that are not considered in the classical DLVO theory. AFM results revealed that spores manifested stronger attraction interactions to stainless steel compared to their vegetative cells counterparts due to lower energy barrier as predicted by both the theoretical approaches as well as the higher hydrophobicity on the spore surfaces.

Controlled biomineralization of electrospun poly(ε-caprolactone) fibers to enhance their mechanical properties.

Electrospun polymeric fibers have been investigated as scaffolding materials for bone tissue engineering. However, their mechanical properties, and in particular stiffness and ultimate tensile strength, cannot match those of natural bones. The objective of the study was to develop novel composite nanofiber scaffolds by attaching minerals to polymeric fibers using an adhesive material - the mussel-inspired protein polydopamine - as a "superglue".

TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation.

The actin cytoskeleton in eukaryotic cells undergoes drastic rearrangement during mitosis. The changes to the actin cytoskeleton are most obvious in the adherent cells, where the actin stress fibres are disassembled, and the cortical actin network becomes more prominent with concomitant increase in cell rigidity as cells round up and enter mitosis. Although the regulatory connections between the actin cytoskeleton and the early mitotic events are apparent, the mechanisms that govern these links are not well understood.

Mussel inspired protein-mediated surface modification to electrospun fibers and their potential biomedical applications.

Mussel inspired proteins have been demonstrated to serve as a versatile biologic adhesive with numerous applications. The present study illustrates the use of such Mussel inspired proteins (polydopamine) in the fabrication of functionalized bio-inspired nanomaterials capable of both improving cell response and sustained delivery of model probes. X-ray photoelectron spectroscopy analysis confirmed the ability of dopamine to polymerize on the surface of plasma-treated, electrospun poly(ε-caprolactone) (PCL) fiber mats to form polydopamine coating.

Fabrication of large pores in electrospun nanofibrous scaffolds for cellular infiltration: a review.

In the past decade, considerable effort has been made to construct biomimetic scaffolds from electrospun nanofibers for engineering different tissues. However, one of the major concerns with electrospun nanofibrous scaffolds is that the densely arranged architecture of fibers and small pores or voids between fibers hinder efficient cellular infiltration or prevent three dimensional (3D) cellular integration with host tissue in vivo after implantation. To overcome this problem, many concepts or strategies applicable during the electrospinning or post-electrospinning procedures have been proposed to enlarge pore size of electrospun scaffolds.

[In vivo and in vitro research study of the influence on placenta angiogenesis by gene silencing of netrin-1].

Objective: To study influence on angiogenesis of placenta by gene silencing of netrin-1.

Methods: Netrin-1 gene in human umbilical vein endothelial cells (HUVEC) and placenta of pregnant rats were silenced by RNA interference. The following methods were used in this study, including the phenytetrazoliumromide (MTT) for viability, clone formation for proliferation, transwell for migration, and tube formation for angiogenesis in vitro.

Reduced expression of netrin-1 is associated with fetal growth restriction.

The objective of this study was to investigate the expression of netrin-1 in placenta from patients with fetal growth restriction (FGR) and its effect on the viability and apoptosis of human placental microvascular endothelial cells. Thirty-three patients with FGR (including eighteen severe cases) and twenty-four normal late pregnant women were investigated. The expression of netrin-1 in placental tissues was detected by employing immunohistochemistry, real-time PCR, and Western blotting.

Effect of different concentrations of neogenin on proliferation, apoptosis and related proliferative factors in human trophoblasts.

The underlying effect of different concentrations of neogenin on proliferation, apoptosis and the related proliferative factors in human trophoblasts was explored in order to understand the function of neogenin during placentation. TEV-1 cell line was cultured and the expression of netrin-1 was detected by using indirect cellular immunofluorescence. Exponentially growing TEV-1 cells were treated by different concentrations of neogenin (0, 1, 5, 10, 50 ng/mL) for 24 h.

Evaluation of "J"-shaped uterine incision during caesarean section in patients with placenta previa: a retrospective study.

This study evaluated the efficacy and safety of "J"-shaped uterine incision for caesarean section for patients diagnosed with placenta previa. A total of 55 consecutive cases of placenta previa treated in Union Hospital were retrospectively analyzed over a period of two years and 10 months. The subjects were divided into two groups with respect to the uterine incision.

Enhanced biological stability of collagen with incorporation of PAMAM dendrimer.

The crosslinking methods of collagen using glutaraldehyde (GTA) and 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) are frequently performed in biomedical applications, but both methods still have their own disadvantages, including the GTA cytotoxicity and low degree of EDC/NHS crosslinking. In this study, we incorporated polyamidoamine (PAMAM) dendrimer with surface amine groups into the two aforementioned crosslinking methods to improve the biostability and structural integrity of collagen. Fifty micromolar of dendrimer concentration was found to have negligible in vitro cytotoxicity and was used for EDC and GTA crosslinking of collagen.

An aligned nanofibrous collagen scaffold by electrospinning and its effects on in vitro fibroblast culture.

Fabrication of nanofibrous scaffolds with well-defined architecture mimicking native extracellular matrix analog has significant potentials for many specific tissue engineering and organs regeneration applications. The fabrication of aligned collagen nanofibrous scaffolds by electrospinning was described in this study. The structure and in vitro properties of these scaffolds were compared with a random collagen scaffold.