Integration of transcriptomics and metabolomics to reveal crizotinib-induced liver injury in mice.

Drug-induced liver injury is a major cause of acute liver failure. Crizotinib is a first-line treatment for patients with cellular-mesenchymal epithelial transition factor (c-MET), anaplastic lymphoma kinase (ALK), and ROS proto-oncogene 1 (ROS1)-positive non-small cell lung cancer. Although some patients treated with crizotinib experience hepatic adverse effects, the underlying mechanisms remain unclear.

Genetic analysis identifies key loci for traits and resistance in Qinghai plateau wheat F populations.

The Qinghai Plateau's high-altitude conditions present significant challenges for wheat cultivation, demanding varieties with enhanced adaptability and stress resistance. However, the genetic basis for key traits like grain yield and stress tolerance in wheat adapted to these conditions remains poorly understood. This knowledge is crucial for developing wheat varieties that can thrive in the harsh environment of the Qinghai Plateau.

Analysis of factors influencing lung cancer in patients with type 2 diabetes mellitus.

This study examined factors influencing lung cancer in patients with type 2 diabetes mellitus (T2DM). A total of 111 patients with T2DM diagnosed with stage IIIB or IV lung cancer and treated at Jining No. 1 People's Hospital between January 2015 and December 2020 were included.

Molecular dynamics simulation of the aggregation and folding mechanism of α-synuclein 47-56 in core peptide fragments induced by α-synuclein pentamer template.

The misfolding of intrinsically disordered α-synuclein protein, which can form β-sheet-rich fibrillar amyloid structures, is closely associated with Parkinson's disease (PD). The peptide α-synuclein 47-56 has been identified as the toxic core of α-synuclein and plays a pivotal role in the aggregation and misfolding processes of this protein. Investigating the template induction behavior of this peptide is crucial for elucidating the molecular mechanisms underlying α-synuclein misfolding and aggregation.

Dynamic landscape and regulation of mC methylation in human tissues.

5-methylcytosine (mC) is a prevalent RNA modification that has various impacts on mRNA fate. Here, we generated 30 single-base resolution RNA mC methylomes and revealed the dynamic nature of mC of heart, muscle, lung, esophagus, stomach, pancreas, colon, jejunum, and rectum from 7 adult human individuals using RNA-BisSeq. Based on clustering analysis, the heart and muscle formed one cluster, while the remaining tissues constituted another cluster.

Demystifying the cGAS-STING pathway: precision regulation in the tumor immune microenvironment.

The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling pathway serves as an immune sentinel for cytosolic DNA, recognizing double-stranded DNA (dsDNA) derived from abnormally localized nuclear DNA or mitochondrial DNA (mtDNA), and plays a pivotal role in innate immune responses and tumor immune surveillance. Conventional antitumor therapies induce genomic instability and mitochondrial stress, leading to the release of nuclear DNA and mtDNA into the cytosol, thereby activating the cGAS-STING pathway. This activation triggers the production of type I interferons (IFN-I) and pro-inflammatory cytokines, which reshape the tumor immune microenvironment (TIME).

Which Types of Patients With Extensive-Stage Small Cell Lung Cancer Benefit From Radiotherapy? A Retrospective Study Integrating Machine Learning With the SEER Database and a Chinese Cohort.

IntroductionAccurate machine learning-based prognostic models for the diagnosis and treatment of extensive-stage small cell lung cancer (ES-SCLC) are currently lacking, and the role of radiotherapy in ES-SCLC remains a subject of ongoing debate.MethodsThis study used data from the Surveillance, Epidemiology, and End Results (SEER) database of patients diagnosed with ES-SCLC. Cox regression analysis was performed to identify the key prognostic factors.

The integrated single-cell analysis interpret the lactate metabolism-driven immune suppression in triple-negative breast cancer.

Background: Individuals with triple-negative breast cancer (TNBC) exhibit elevated lactate levels, which offers a valuable lead for investigating the molecular mechanisms underlying the tumor microenvironment (TME) and identifying more efficacious treatments.

Methods: TNBC samples were classified based on lactate-associated genes. A single-cell transcriptomic approach was employed to examine functional differences across cells with varying lactate metabolism.

Promethazine for nausea and vomiting prevention after gynaecological laparoscopic surgery: A randomized controlled trial.

Postoperative nausea and vomiting (PONV) represent significant concerns for patients undergoing surgical procedures, as these symptoms greatly impact their postoperative experience. Among female patients undergoing laparoscopic surgery, the incidence of PONV is estimated to be approximately 45%. Moreover, for those individuals who have not undergone preventive treatment, the risk of experiencing PONV can be as high as 80%.

Probing the biological efficacy and mechanistic pathways of natural compounds in breast cancer therapy via the Hedgehog signaling pathway.

Breast cancer (BC) is one of the most prevalent malignant tumors affecting women worldwide, with its incidence rate continuously increasing. As a result, treatment strategies for this disease have received considerable attention. Research has highlighted the crucial role of the Hedgehog (Hh) signaling pathway in the initiation and progression of BC, particularly in promoting tumor growth and metastasis.

Value of multi-modal ultrasound in evaluation of the accessory renal artery.

Background: The evaluation of the accessory renal artery (ARA) holds clinical significance in the effective intervention of resistant hypertension and renal vascular-related surgical procedures. Multi-modal ultrasound is a non-invasive, secure, and real-time imaging modality, especially useful in patients with renal impairment. Nevertheless, few studies have focused on the value of multi-modal ultrasound in the assessment of the ARA.

Characteristics and influencing factors of microplastics entering human blood through intravenous injection.

The presence of microplastics in the human body and their potential health risks have drawn widespread attention in recent years. Microplastics have been detected in human blood, though their pathways of entry remain unclear. This study employed Raman spectroscopy and energy dispersive spectroscopy to evaluate the microplastic release characteristics of intravenous medical devices, aiming to investigate the influencing factors and the risk of microplastics entering the bloodstream.

Transcutaneous Electrical Acupoint Stimulation Improves Postoperative Nutrition and Promotes Early Recovery of Gastrointestinal Function in Patients with Colorectal Cancer.

Objective: To investigate the effect of transcutaneous electrical acupoint stimulation (TEAS) on the postoperative nutritional status and recovery of gastrointestinal function in colorectal cancer patients.

Methods: Sixty-five patients with ASA grade I-II, undergoing laparoscopic radical colorectal cancer surgery under elective general anesthesia were selected. They were divided into two groups according to the random number table method: the TEAS group (T group) and the sham stimulation group (S group).

The efficacy and safety of patient-controlled intravenous analgesia with esketamine after total hip arthroplasty: a randomized controlled trial.

Purpose: To evaluate the efficacy and safety of esketamine-based patient-controlled intravenous analgesia following total hip arthroplasty.

Methods: A total of 135 total hip arthroplasty patients were randomly assigned to one of the three treatment groups: esketamine, sufentanil or continuous fascia iliaca compartment block (FICB) group. The primary endpoint was the postoperative visual analogue scale (VAS) pain scores at rest and on movement.

Elucidating the Role of Estrogen Effects in Leukemia: Insights from Single-Cell RNA Sequencing and Mendelian Randomization.

Epidemiological studies have confirmed the potential role of estrogen effects in influencing the development and outcome of leukemia. Estrogen effects are increasingly attracting research interest for their potential antitumor effects beyond gynecological tumors. However, their causal relationship remains unclear.

Upregulation of ISG15 induced by MAPT/tau accumulation represses autophagic flux by inhibiting HDAC6 activity: a vicious cycle in Alzheimer disease.

Alzheimer disease (AD), a prevalent neurodegenerative condition in the elderly, is marked by a deficit in macroautophagy/autophagy, leading to intracellular MAPT/tau accumulation. While ISG15 (ISG15 ubiquitin like modifier) has been identified as a regulator of selective autophagy in ataxia telangiectasia (A-T), its role in AD remains unexplored. Our study reveals elevated ISG15 levels in the brains of patients with sporadic AD and AD models and .

Regional analgesia techniques following nephrectomy: a systematic review and network meta-analysis.

Introduction: This network meta-analysis (NMA) was performed to assess the relative efficacy and safety of various regional analgesic techniques used in patients undergoing partial nephrectomy or nephrectomy.

Evidence Acquisition: Randomized controlled trials (RCTs) evaluating different regional analgesia techniques in patients who underwent partial nephrectomy or nephrectomy were retrieved from databases, including PubMed, Embase, Web of Science, and the Cochrane Library, from inception to January 2024. The NMA was made by using Stata 15.

Overexpression of TECPR1 improved cognitive function of P301S-tau mice via activation of autophagy in the early and late process.

Autophagy disorders in AD patients and animal models were well known, however, the effect of P301S-tau on autophagy is not clear. Here, we found that autophagy related protein Tectonin Beta-Propeller Repeat-Containing Protein 1 (TECPR1) decreased in the hippocampus of P301S-tau transgenic mice by proteomics, which was proved in vivo and in vitro, and P301S-tau induced autophagic deficits in early and late process. TECPR1 overexpression attenuated P301S-tau induced autophagy defects via promoting autophagosome generation and autophagosome and lysosomes fusion.

Diversified applications of hepatocellular carcinoma medications: molecular-targeted, immunotherapeutic, and combined approaches.

Hepatocellular carcinoma (HCC) is one of the primary forms of liver cancer and is currently the sixth most prevalent malignancy worldwide. In addition to surgical interventions, effective drug treatment is essential for treating HCC. With an increasing number of therapeutic drugs for liver cancer undergoing clinical studies, the therapeutic strategies for advanced HCC are more diverse than ever, leading to improved prospects for HCC patients.

Interactions between hedgehog signaling pathway and the complex tumor microenvironment in breast cancer: current knowledge and therapeutic promises.

Breast cancer ranks as one of the most common malignancies among women, with its prognosis and therapeutic efficacy heavily influenced by factors associated with the tumor cell biology, particularly the tumor microenvironment (TME). The diverse elements of the TME are engaged in dynamic bidirectional signaling interactions with various pathways, which together dictate the growth, invasiveness, and metastatic potential of breast cancer. The Hedgehog (Hh) signaling pathway, first identified in Drosophila, has been established as playing a critical role in human development and disease.

Microplastic pollution differences in freshwater river according to stream order: Insights from spatial distribution, annual load, and ecological assessment.

Microplastic (MP) pollution has become a pressing concern in global freshwater ecosystems because rivers serve as essential channels for the transport of terrestrial debris to the ocean. The current researches mostly focus on the large catchments, but the impact on the small catchments remains underexplored. In this study, we employed Strahler's stream order classification to delineate the catchment structure of the Beijiang River in South China.

BGP-15 alleviates LPS-induced depression-like behavior by promoting mitophagy.

The high prevalence of major depressive disorder (MDD) frequently imposes severe constraints on psychosocial functioning and detrimentally impacts overall well-being. Despite the growing interest in the hypothesis of mitochondrial dysfunction, the precise mechanistic underpinnings and therapeutic strategies remain unclear and require further investigation. In this study, an MDD model was established in mice using lipopolysaccharide (LPS).

Characterizing the photodegradation-induced release of volatile organic compounds from bottled water containers.

While plastic water bottles are known to potentially release various volatile organic compounds (VOCs) when exposed to light, existing knowledge in this field remains limited. In this study, we systematically examined the composition, yield, and toxicity of VOCs released from six plastic containers obtained from different continents under UV-A and solar irradiation. After light exposure, all containers released VOCs, including alkanes, alkenes, alcohols, aldehydes, carboxylic acids, aromatics, etc.

Ultrasound-Base Radiomics for Discerning Lymph Node Metastasis in Thyroid Cancer: A Systematic Review and Meta-analysis.

Purpose: Ultrasound is the imaging modality of choice for preoperative diagnosis of lymph node metastasis (LNM) in thyroid cancer (TC), yet its efficacy remains suboptimal. As radiomics gains traction in tumor diagnosis, its integration with ultrasound for LNM differentiation in TC has emerged, but its diagnostic merit is debated. This study assesses the accuracy of ultrasound-integrated radiomics in preoperatively diagnosing LNM in TC.