A fully digital approach to autonomous robot-assisted immediate implant and interim restoration placement in the maxillary anterior esthetic zone: A case series.

Statement Of Problem: Immediate implant and interim restoration placement in the maxillary anterior esthetic zone presents challenges in achieving optimal implant positioning, esthetics, and peri-implant tissue stability. A more accurate and efficient clinical workflow is needed to address these demands.

Purpose: This study aimed to establish a fully digital workflow that combines autonomous robotic-assisted immediate implant placement and prefabricated interim restorations in the maxillary anterior esthetic region and to evaluate its accuracy and short-term clinical outcomes.

tRF-21LeuTAA Promotes Oxidative Stress by Altering Glutathione Metabolic Enzymes to Support Prostate Cancer Progression.

Transfer RNA (tRNA)-derived fragments (tRFs) are a class of small non-coding RNAs that have recently been implicated in various physiological and pathological processes, including tumor development and progression. Identification of the roles of tRFs in cancer could provide insights into tumor biology and treatment strategies. Here, we found that tRF-21LeuTAA supported progression of prostate cancer by regulating glutathione metabolic enzymes and promoting reactive oxygen species (ROS) accumulation.

A Study on the Impact of HPV Genotype Infection Patterns on the Occurrence of High-Grade Squamous Intraepithelial Lesion.

Objective: To investigate the differential risk of high-grade squamous intraepithelial lesion or worse (HSIL+) associated with single versus co-infection patterns of high-risk human papillomavirus (HR-HPV) genotypes.

Methods: In this retrospective cohort study, 10,570 patients with abnormal ThinPrep cytology test results and/or HR-HPV infection who underwent colposcopy-guided cervical biopsy at Wuhan Children's Hospital (May 2021-May 2023) were enrolled. Histopathological diagnosis served as the gold standard.

Novel opportunity of treatment for psycho-cardiologic disease by gut microbiome.

Cardiovascular disease (CVD) patients combined with depression, anxiety, and other psychiatric disorders are becoming a common occurrence. There are many comorbid mechanisms, and CVD patients with psychiatric disorders have poor prognosis. Several studies have shown that dysbiosis and metabolite alterations in the gut were major risk factors for CVD and psychosomatic disorders.

Clinicopathologic and Molecular Study of TFEB-altered Renal Cell Carcinomas: Tumors With Frequent PDL1 Expression.

TFEB-altered renal cell carcinoma (RCC) included TFEB-rearranged and TFEB-amplified RCC with unclear clinicopathological features and treatment options. Cases of TFEB-altered RCCs were collected. Fourteen cases showed TFEB rearrangement.

Tolerance Induced by Porphyromonas gingivalis Altered Inflammatory Responses in Mice.

Tolerance refers to a hyporesponsiveness toward repeated stimulations with bacteria and their virulence factors, which might exist in the development of periodontitis. To identify the roles of tolerance induced by Porphyromonas gingivalis (P. gingivalis) in periodontitis, an original tolerized mice model was established by high-dose of oral P.

LINC00654 promotes ovarian cancer progression by facilitating nuclear export of HuR and stabilizing oncogenic mRNAs.

Ovarian cancer (OC) remains a significant challenge in oncology due to its late diagnosis and poor prognosis. Emerging evidence suggests that long non-coding RNAs (lncRNAs) play critical roles in cancer biology. Herein, we reported that LINC00654 was highly expressed in OC tissues and correlated with poor patient prognosis.

Adverse impact of paternal age on embryo euploidy: insights from retrospective analysis and interpretable Machine learning.

The trend of delayed childbearing has increased the average age of parents, with the impact of paternal age on embryo euploidy remaining controversial. Therefore, this study aimed to investigate the impact of paternal age on embryo euploidy using retrospective clinical data and interpretable machine learning. This retrospective study included 960 couples and 4,718 embryos undergoing preimplantation genetic testing for aneuploidy (PGT-A).

A qualitative study on rumination in young and middle-aged ovarian cancer survivors.

Purpose: Ovarian cancer can have a significant psychological impact on young and middle-aged patients who are often prone to recurring changes in mood that can lead to rumination. This phenomenological qualitative study aimed to explore rumination in young and middle-aged ovarian cancer survivors.

Methods: From August to November 2023, 21 young and middle-aged ovarian cancer survivors who were treated at a tertiary general hospital in Changsha, Hunan Province, China were selected using purposive sampling.

Endoscopic retrograde cholangiopancreatography for the diagnosis and treatment of biliary Fasciolopsis buski: A rare case report and literature review.

Rationale: Fasciolopsis buski can cause fasciolopsiasis, which usually parasitizes the duodenum and jejunum in humans, the biliary tract is rarely reported. However, as most infections are asymptomatic or present with nonspecific symptoms, they are often not diagnosed or sometimes even misdiagnosed.

Patient Concerns: We report the rare case of a 59-year-old male who was admitted to our hospital with a history of recurrent and unexplained abdominal pain for ≈11 years and worsening symptoms for 5 days.

Age-related changes in lncRNA expression in sperm.

Background: Over the past few decades, the age of parenthood has become increasingly delayed. With increasing age, offspring health may be affected; these effects can include an increased risk of genetic disorders and diseases related to neurodevelopment and cognitive function, such as autism and schizophrenia. Long noncoding RNAs (lncRNAs) play important roles in reproductive processes and epigenetics, regulating gene expression through epigenetic modifications, transcriptional regulation, and posttranscriptional control.

Comparison of pregnancy outcomes for high morphological scoring mosaic vs. low morphological scoring euploid embryos: a retrospective cohort study.

Background: Mosaic embryos have been proven to be capable of resulting in live births and have become an option for embryo transfer under certain circumstances. Recent guidelines suggested that embryo morphological scoring should be taken into consideration when selecting mosaic embryos for transfer. Therefore, we introduce a hypothesis that a high morphological scoring mosaic embryo is a better choice compared to a low morphological scoring euploid embryo.

Simultaneous determination of four PDE5 inhibitors and metabolites in rat plasma by UPLC-MS/MS.

Herein, we developed a sensitive UPLC-MS/MS method for the simultaneous determination of four PDE5 inhibitor and twelve metabolites in rat plasma. A total of 16 chemicals were separated on gradient elution with mobile phase A (0.1 % formate acetonitrile) and mobile phase B (0.

Ductal or Ngn3 cells do not contribute to adult pancreatic islet beta-cell neogenesis in homeostasis.

The adult pancreatic ducts have long been proposed to contain rare progenitors, some of which expressing Ngn3, that generate new beta cells in endocrine-islet homeostasis. Due to their postulated rarity and the lack of definitive markers, the existence or absence of ductal endocrine progenitors remains unsettled despite many studies. Genetic lineage tracing of ductal cells or Ngn3 cells with currently available CreER drivers has been complicated by off-target labeling of pre-existing beta cells.

Intestinal secretory differentiation reflects niche-driven phenotypic and epigenetic plasticity of a common signal-responsive terminal cell.

Enterocytes and four classic secretory cell types derive from intestinal epithelial stem cells. Based on morphology, location, and canonical markers, goblet and Paneth cells are considered distinct secretory types. Here, we report high overlap in their transcripts and sites of accessible chromatin, in marked contrast to those of their enteroendocrine or tuft cell siblings.

Unraveling an excited state intramolecular double proton transfer pathway in 2,5-bis(benzoxazole-2-yl)benzene-1,4-diol derivatives.

Organic molecules exhibiting excited-state intramolecular double proton transfer (ESIDPT) have garnered significant research interest, largely driven by their prevalence in nature and the unique luminescent properties associated with this phenomenon. This study presents a detailed theoretical investigation into the dynamic mechanism of both single and dual cooperative Proton transfer (PT) in the first excited singlet (S) state of 2,5-bis(benzoxazole-2-yl)-1,4-dihydroxybenzene derivatives. The analysis meticulously incorporates solvent effects, specifically those of dichloromethane, to provide a comprehensive understanding of the PT processes.

The Novel Dual GIP and GLP-1 Receptor Agonist Tirzepatide Attenuates Colon Cancer Development by Regulating Glucose Metabolism.

Colorectal cancer (CRC) is a leading cause of cancer mortality while diabetes is a recognized risk factor for CRC. Here we report that tirzepatide (TZP), a novel polypeptide/glucagon-like peptide 1 receptor (GIPR/GLP-1R) agonist for the treatment of diabetes, has a role in attenuating CRC growth. TZP significantly inhibited colon cancer cell proliferation promoted apoptosis in vitro and induced durable tumor regression in vivo under hyperglycemic and nonhyperglycemic conditions across multiple murine cancer models.

Relationship between neurotic personality traits with symptoms of post-traumatic stress disorder in breast cancer patients: with the mediation of self-efficacy and negative coping.

Purpose: The present study was conducted to determine the relationship between neurotic personality traits and symptoms of post-traumatic stress disorder (PTSD) in breast cancer patients, with the mediation of self-efficacy and negative coping.

Methods: A cross-sectional study design was used. A total of 328 breast cancer patients admitted to two tertiary hospitals in Dali, China, from July 2023 to January 2024 were included.

Efficient C25-Hydroxylation of Vitamin D Utilizing an Artificial Self-Sufficient Whole-Cell Cytochrome P450 Biocatalyst.

Cytochrome P450 enzymes (P450s) are promising candidates for the biosynthesis of 25-hydroxyvitamin D (25(OH)VD). However, their industrial application is limited by challenges, such as low stability, inefficient catalysis, and uncoupling reactions. The construction of self-sufficient P450s offers a strategic solution to these limitations, but requires linker optimization to regulate interdomain conformational dynamics.

A PheWAS approach to identify associations of GBA1 variants with comprehensive phenotypes beyond neurological diseases.

Given the established association between numerous GBA1 variants and specific neurological diseases, we extended the exploration by a phenome-wide association study to assess the impact of GBA1 variants on a wider spectrum of health-related traits. We identified 41 phenotypes associated with GBA1 variants, 39 of which were unreported, including 21 non-neurological and 20 neurological phenotypes. Based on variant-level association tests, we found beyond the neurological phenotypes particularly decreased gray-white matter contrast measures across 13 distinct brain regions, the non-coding variant rs9628662 was associated with six non-neurological traits such as hypermetropia.

Sea buckthorn berries alleviate ulcerative colitis via regulating gut Faecalibaculum rodentium-mediated butyrate biosynthesis.

Background: The gut microbiota is firmly associated with the progression of ulcerative colitis (UC). Beneficial microbial metabolites, such as butyrate, exert vital roles in maintaining intestinal homeostasis. The Sea buckthorn berry is a traditional Chinese medicine homologous to food and medicine which is widely applied in the prevention and treatment of UC in clinic practice.

Early detection of Parkinson's disease through multiplex blood and urine biomarkers prior to clinical diagnosis.

Blood and urine biomarkers are commonly used to diagnose and monitor chronic diseases. We initially screened 67 biomarkers, including 4 urine biomarkers and 63 blood biomarkers, and identified 13 blood biomarkers significantly associated with Parkinson's disease (PD). Among these, we discovered three novel markers demonstrating strong associations: phosphate (P = 1.

Niche-driven phenotypic plasticity and cis-regulatory dynamics of a revised model for intestinal secretory differentiation.

Enterocytes and four secretory cell types derive from stem cells located in intestinal crypts. Whereas secretory goblet and Paneth cells have long been considered distinct, we find high overlap in their transcripts and sites of accessible chromatin, in marked contrast to those of sibling enteroendocrine or tuft cells. Mouse and human goblet and Paneth cells express extraordinary fractions of selective antimicrobial genes, reflecting specific and variable gene responses to local niche signals.