Degradable Polyketone Thermoplastic Elastomers with Biomass Sourced Composition.

Polyolefin elastomers (POEs) serve as versatile materials bridging rubber and plastic properties across a variety of industrial processes, but their environmental persistence stemming from inert hydrocarbon composition raises ecological concerns. Here, we present a sustainable synthesis of degradable thermoplastic elastomers (TPEs) through the carbonylative polymerization of ethylene and biomass-derived polar monomers (PMs). A cost-effective and scalable nickel catalyst has overcome the dual poison to metal center resulted from polar acid or ester motif and CO, enabling the synthesis of high-molecular-weight polyketone materials with moderate strain recovery (SR) values ranging from 27% to 61%.

PRRSV vector vaccine based on VEEV-VSVG recombinant replicon elicits efficient immune responses in piglets.

Porcine Reproductive and Respiratory Syndrome (PRRS) is one of the most significant viral infectious diseases affecting the global swine industry. Eradicating PRRS remains a formidable challenge due to the high genetic diversity and the complexity of the host immune response. Currently, no commercial vaccine provides complete protection against Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection.

Clinicopathologic and Molecular Study of TFEB-altered Renal Cell Carcinomas: Tumors With Frequent PDL1 Expression.

TFEB-altered renal cell carcinoma (RCC) included TFEB-rearranged and TFEB-amplified RCC with unclear clinicopathological features and treatment options. Cases of TFEB-altered RCCs were collected. Fourteen cases showed TFEB rearrangement.

Evaluation of precursor MicroRNA (pre-miRNA) as a powerful tool for robust CHO production cell line platform development.

Chinese hamster ovary (CHO) cells are the most widely used host for the commercial production of recombinant therapeutic proteins. The rapidly growing demand for large quantities of biologics at controllable cost-of-goods requires continuous cell engineering and process optimization of the CHO host cells. MicroRNAs (miRNAs) have been shown to enhance recombinant protein production in CHO cells.

Secretagogin Downregulation Impairs Nerve Cell Migration in Hirschsprung Disease via Inhibition of the LEF-1/NCAM1 Axis.

Hirschsprung disease (HSCR) is a common peripheral neurodevelopmental disorder and impaired enteric neural crest cell migration is one of the key factors. Secretagogin (SCGN) has been demonstrated to play a critical role in the rostral migratory stream during central nerve regeneration. However, there is a paucity of knowledge on the role of SCGN in enteric neural crest cell migration.

Carrier-Free Nanomedicine Based on Celastrol and Methotrexate for Synergistic Treatment of Breast Cancer via Folate Targeting.

Purpose: To address celastrol(Ce)'s efficacy and toxicity challenges in breast cancer, we first developed a carrier-free, self-targeting nanosystem with synergistic anti-tumor action by leveraging methotrexate (MTX)'s intrinsic folate moiety for active tumor targeting.

Methods: Ce-MTX nanoparticles (NPs) were prepared using a solvent precipitation method, with formulation parameters optimized. Characterization included particle size, polydispersity index (PDI), encapsulation efficiency (EE), loading efficiency (LE), and TEM.

Risk factors and prognosis of post-surgical acute kidney injury in elderly patients based on the MIMIC-IV database.

Objective: To investigate the risk factors and prognosis of acute kidney injury (AKI) in elderly patients after surgery based on the Medical Information Mart for Intensive Care IV (MIMIC-IV).

Methods: A retrospective analysis was conducted using MIMIC-IV data pertaining to aged postoperative patients. By logistic regression analysis models, Kaplan-Meier survival curve and Cox proportional hazards regression model.

Biliary atresia: Rotavirus amplification of lipopolysaccharide/toll-like receptor 4 by mediating MMP7 upregulation through NF-κB.

Background: Matrix metalloproteinase-7 (MMP7), which is expressed primarily by biliary epithelial cells (BECs), has been shown to promote biliary atresia (BA). However, the mechanism by which elevated MMP7 expression is induced in BA remains unclear.

Methods: Mouse extrahepatic BECs were used to investigate MMP7 overexpression induced by rhesus rotavirus (RRV) and lipopolysaccharide (LPS).

Intensification of 2'-Fucosyllactose biosynthesis pathway by using a novel fucosyltransferase from .

Introduction: 2'-Fucosyllactose (2'-FL) is an oligosaccharide that can be synthesized in the human body and is known for its health-promoting and prebiotic effects. The biosynthesis of 2'-FL using microorganisms has received attention recently due to its increased application in nutritional and medical infant formulations.

Methods: This work attempts the new application of α-1,2-fucosyltransferase (FutCB) in the synthesis of 2'-FL in ().

The causal association between circulating metabolites and Alzheimer's disease: a systematic review and meta-analysis of Mendelian randomization studies.

Introduction/objective: Some Mendelian randomization (MR) studies have found that there may be a genetic causal relationship between circulating metabolites and Alzheimer 's disease (AD), but the strength of evidence and the direction of association are not always consistent. In this study, a systematic review and meta-analysis of all the literature using MR methods to study the causal relationship between metabolites and AD was conducted to enhance the robustness and correlation of predicting genetic causality.

Methods: We conducted a comprehensive review of Mendelian randomization (MR) studies which are within the timeframe of all years to 20 December 2023.

Development and Performance of Negative Ion Functional Blended Yarns and Double-Sided Knitted Fabrics Based on ZnO/TM/PET Fiber.

Zinc oxide-modified tourmaline-based negative ion polyester fiber (ZnO/TM/PET), as a new functional fiber with excellent negative ion emission characteristics, is of great significance to human health, and its industrial application needs to be expanded and promoted. In this paper, using zinc oxide, tourmaline, and polyethylene terephthalate as the main raw materials, ZnO/TM/PET negative ion functional fiber with 5% ZnO/TM composites was prepared. Then, it was blended with cotton fiber and interknitted with wool yarn and spandex yarn, from which we developed five kinds of negative ion polyester/cotton-blended yarn and four different kinds of knitted double-sided fabric using different equipment and process parameters.

Deep learning in nuclear medicine: from imaging to therapy.

Background: Deep learning, a leading technology in artificial intelligence (AI), has shown remarkable potential in revolutionizing nuclear medicine.

Objective: This review presents recent advancements in deep learning applications, particularly in nuclear medicine imaging, lesion detection, and radiopharmaceutical therapy.

Results: Leveraging various neural network architectures, deep learning has significantly enhanced the accuracy of image reconstruction, lesion segmentation, and diagnosis, improving the efficiency of disease detection and treatment planning.

Does thoracoscopic repair of type C esophageal atresia require emergency treatment?

Background: Thoracoscopic repair of esophageal atresia (EA) with tracheoesophageal fistula (TEF) has been performed with increasing frequency. Although many children have underwent surgery within three days after birth, the optimal timing for operation remains undetermined. This study aimed to investigate the appropriate timing for thoracoscopic repair of type C EA and its mid-term clinical outcomes.

Magnetic Compression Anastomosis for Anorectal Malformations: Feasibility and Efficacy in Swine Model and Clinical Observation.

Background: Patients with anorectal malformations (ARM) commonly experience post-operative bowel dysfunctions due to inherent neuromuscular dysplasia and surgical damage to extraluminal structures. Magnetic compression anastomosis (MCA) minimizes injury to the perirectal neurovascular bundle and sphincter muscles via an endoluminal approach. We evaluated the preclinical feasibility and healing efficacy of a novel MCA device in a swine model of ARM and applied the device to an ARM patient.

Comparison of surgical results and technical performance between robotic and laparoscopic approaches for Kasai portoenterostomy in biliary atresia: a multicenter retrospective study.

Background: Many variables, including age at surgery, disease type, surgical approaches and perioperative management factors have been demonstrated to influence efficacy in BA infants, however, the effect of surgical performance remains unclear. The objective of this retrospective study was to compare the postoperative efficacy and surgical performance of robotic (RKPE) versus laparoscopic Kasai portoenterostomy (LKPE) for BA.

Methods: Between October 2018 and June 2023, 158 type III BA patients undergoing minimally invasive surgery (RKPE = 66, LKPE = 92) were included in this multicenter retrospective study.

Targeting APE1: Advancements in the Diagnosis and Treatment of Tumors.

With the emergence of the precision medicine era, targeting specific proteins has emerged as a pivotal breakthrough in tumor diagnosis and treatment. Apurinic/apyrimidinic Endonuclease 1 (APE1) is a multifunctional protein that plays a crucial role in DNA repair and cellular redox regulation. This article comprehensively explores the fundamental mechanisms of APE1 as a multifunctional enzyme in biology, with particular emphasis on its potential significance in disease diagnosis and strategies for tumor treatment.

Tertiary lymphoid structures potentially promote immune checkpoint inhibitor response in SMARCB1-deficient medullary renal cell carcinoma.

The WHO's classification of renal cell carcinoma (RCC) has identified loss of SMARCB1 as one of the driven mutations. Despite intensive postoperative interventions, the prognosis for SMARCB1-deficient medullary RCC remains poor, indicating insufficiency in current therapy. Herein, we reported the treatment outcomes of five patients with metastatic SMARCB1-deficient medullary RCC and molecular correlates.

A primary intracranial neuroepithelial neoplasm with novel TCF3::BEND2 fusion: a case report.

Astroblastoma, MN1-altered, is a rare circumscribed glial neoplasm that is composed of round, cuboidal, orcolumnar cells with astroblastic perivascular pseudorosettes, often associated with MN1::BEND2 and MN1::CXXC5 fusions. Atroblastoma-like gliomas harbouring EWSR1::BEND2 have been reported that they defined an epigenetically distinct subtype of astroblastoma. We report a case of a 19-year-old female with an intracranial neuroepithelial tumor featuring a novel TCF3::BEND2 fusion.

Joint metabolomics and transcriptomics analysis systematically reveal the impact of MYCN in neuroblastoma.

The limited understanding of the molecular mechanism underlying MYCN-amplified (MNA) neuroblastoma (NB) has hindered the identification of effective therapeutic targets for MNA NB, contributing to its higher mortality rate compared to MYCN non-amplified (non-MNA) NB. Therefore, a comprehensive analysis integrating metabolomics and transcriptomics was conducted to systematically investigate the MNA NB. Metabolomics analysis utilized plasma samples from 28 MNA NB patients and 68 non-MNA NB patients, while transcriptomics analysis employed tissue samples from 15 MNA NB patients and 37 non-MNA NB patients.