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Article Abstract
Introduction: 2'-Fucosyllactose (2'-FL) is an oligosaccharide that can be synthesized in the human body and is known for its health-promoting and prebiotic effects. The biosynthesis of 2'-FL using microorganisms has received attention recently due to its increased application in nutritional and medical infant formulations.
Methods: This work attempts the new application of α-1,2-fucosyltransferase (FutCB) in the synthesis of 2'-FL in (). Additionally, knocking out the and genes alongside overexpression of the key gmd, manB, wcaG, and manC genes enhances the availability of the necessary precursors GDP-L-fucose and lactose for the synthesis of 2'-FL.
Results And Discussion: The use of constitutive promoters achieved better control over the production of 2'-FL during fed-batch fermentation. After 64 h of fermentation, the modified strains produced 121.4 g/L 2'-FL with a yield of 1.90 g/L/h, resulting in an impressive 2'-FL output. These results together indicate the potential of large-scale, high-yield production of 2'-FL and form a basis of much more refinement to be done. The next step will focus on maximum substrate utilization, alteration of gene regulation, and improvement of commercial-scale synthesis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075129 | PMC |
| http://dx.doi.org/10.3389/fbioe.2025.1569597 | DOI Listing |
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