Sympathetic Activation Promotes Kidney Fibrosis in Mice via Macrophage-Derived N2ICD-Enriched Extracellular Vesicles.

Persistent overactivation of the renal sympathetic nervous system drives kidney inflammation and fibrosis. Macrophages contribute to fibrogenesis by secreting various pro-fibrogenic mediators. However, whether the sympathetic nervous system regulates renal fibrosis by modulating macrophage-fibroblast interaction remains unclear.

Transcription Factor GmERFA Interacts With GmNFYA and Acts as a Negative Regulator of Seed Fatty Acid Accumulation in Soybean.

Soybean is one of the most important oilseed crops, and its seed oil content directly determines the economic value and industrial applicability worldwide. However, how soybean seed oil accumulation is regulated remains less understood. Here, through RNA-seq analysis and screening for the interacting proteins of a positive oil regulator GmNFYA, we identified an AP2/ERF-type transcription factor GmERFA, which acts as a negative regulator of oil accumulation.

Title: Medium-Term Lag-Response Associations Between PM Exposure and All-Cause Mortality in Valencia and London: A Time-Stratified Case-Crossover Study.

Background: Air pollution is among the top five environmental risk factors for human health worldwide. However, our understanding of the physiological responses to PM exposure over medium-term lag periods remains limited. This study aims to examine the medium-term lag-response associations-using lagging time windows of up to 21 days-between PM exposure and all-cause mortality in Valencia and London from 2002 to 2006.

Multidimensional social signature de-anonymizes low-sensitivity data.

Although privacy protection is increasingly important in the digital age, current measures predominantly focus on safeguarding high-sensitivity data, often overlooking the risks associated with low-sensitivity data. Here, we utilize low-sensitivity anonymous interaction data, which depict the behavioral interaction patterns between users and their contacts, to construct multidimensional social signature for identifying users in anonymous datasets. We investigate the potential of low-sensitivity social signature for user identification and propose a classification framework for measuring feature sensitivity levels.

Preparation and Characterization of Monoclonal Antibodies Against the Porcine Rotavirus VP6 Protein.

Porcine Rotavirus (PoRV), a predominant causative agent of neonatal diarrhea in piglets, shares substantial genetic homology with human rotavirus and represents a considerable threat to both public health and the global swine industry in the absence of specific antiviral interventions. The VP6 protein, an internal capsid component, is characterized by exceptional sequence conservation and robust immunogenicity, rendering it an ideal candidate for viral genotyping and vaccine development. In the present study, the recombinant plasmid pET28a(+)-VP6 was engineered to facilitate the high-yield expression and purification of the VP6 antigen.

Discovery of ()-1,3-Diphenyl-2-Propen-1-One Derivatives as Potent and Orally Active NLRP3 Inflammasome Inhibitors for Colitis.

The pyrin domain-containing protein 3 (NLRP3) inflammasome may be a potential target for the treatment of inflammatory bowel disease (IBD), and inhibiting the activation of the NLRP3 inflammasome is of great significance for the treatment of IBD. In this study, 27 novel chalcone derivatives were designed and synthesized. Enzyme-linked immunosorbent assay (ELISA) analysis revealed that most of the compounds inhibited IL-1β secretion, with exhibiting the most significant activity, showing IC values of 0.

Hardening-off under fluctuating light enhances the photosystem I resistance to excessive fluctuating light in Enhalus acoroides.

Seagrass meadows of Enhalus acoroides are rapidly declining. Transplanting artificially cultured seedlings is considered an efficient method of seagrass restoration. However, excessive fluctuating light (eFL) in the wild is highly likely to be a major threat to the survival of transplanted seedlings of E.

The Weight of Cardiovascular Diseases: Addressing the Global Cardiovascular Crisis Associated with Obesity.

Obesity is a growing global epidemic with significant implications for cardiovascular diseases (CVD). It couples as an independent risk factor and driver for multiple pathways leading to CVDs. Here we examine obesity's impact on CVD and propose actionable strategies.

Direct Ink Writing of Multifunctional Silica-Reinforced Zirconia Aerogels via Electrostatic Attraction-Assisted Co-Gelation.

ZrO aerogels are renowned for their exceptional stability and high positive charge, positioning them as promising candidates for various applications. However, the inherent frangibility and contractibility of ZrO aerogels pose significant challenges in processing and drying intactly. In this study, we introduce a novel approach to fabricate 3D-printed ZrO-based aerogels through electrostatic attraction-assisted cogelation process, utilizing a silica reinforcement and rheological adjustment strategy.

Blocking Fpr3 ameliorates osteoarthritis by inhibiting NLRP3-mediated chondrocyte pyroptosis.

Objective: Osteoarthritis (OA) is a chronic joint disorder that frequently diagnosed in elderly individuals. While formyl peptide receptor 3 (Fpr3) is known to play a significant role in inflammation, its involvement in OA progression and chondrocyte pyroptosis remains unclear.

Methods: Anterior cruciate ligament transection (ACLT) was used to create OA mouse model.

Treatment options in patients with pancreatic cancer: A 10-year multicenter epidemiological investigation in China.

Background: The survival rate of pancreatic cancer is low, and there is a lack of effective treatment.

Aim: To explore the epidemiological characteristics of patients with pancreatic cancer in China and compare multiple chemotherapy regimens at different stages.

Methods: This was a retrospective study conducted from 2005 to 2014, involving six cancer hospitals and eight general hospitals across seven geographical regions of China (East, South, North, Central, Southwest, Northwest, and Northeast).

Suppression of circ_0091761 ameliorates acute myocardial infarction-induced endothelial injury through regulation of miR-1278.

Objective: The main objective of the article was to explore the role of circ_0091761 in acute myocardial infarction (AMI)-induced endothelial injury.

Methods: A cellular model of AMI was constructed by hypoxia-induced HUVECs. miRNAs that may interact with circ_0091761 were recognized by the ENCORI and circular RNA Interactom databases.

Effect of Topical Application of Dexamethasone on CC Chemokine Receptor 3 Expression in Human Pterygium.

To evaluate the effects of preoperative topical demethasone on CC chemokine receptor 3 (CCR3) expression in human pterygium. Forty cases of patients with pterygium were divided into a dexamethasone group and a control group, which were treated topically with 3 g/L tobramycin + dexamethasone or 3 g/L tobramycin eye drops in the surgical eyes 2 weeks before surgery. Pterygium samples were collected from the subjects after pterygium excision surgery and processed for real-time polymerase chain reaction and Western blot to examine CCR3 expression.

Quantitative differential analysis of tsRNAs for forensic body fluid identification: RT-qPCR-based discrimination derived from epithelial cell fluids screening.

Identification of body fluid types at crime scenes is a critical step in forensic science, providing essential context for criminal investigations and the interpretation of evidence. Epigenetic markers, particularly small non-coding RNAs (sncRNAs), have attracted increasing attention in forensic body fluid identification, with various small RNA species demonstrating potential as biomarkers for distinguishing different body fluid types. A novel class of sncRNAs, tRNA-derived small RNAs (tsRNAs), has been detected in various biological samples, yet their potential application in forensic body fluid identification remains unexplored.

Spatio-Temporal Proliferative Heterogeneity of Intra-Organ Endothelial Cells.

Background: Blood vessels play a crucial role in supplying tissues with oxygen and nutrients. The maintenance of normal blood vessel number and integrity requires a continuous supply of new endothelial cells (ECs) through self-replication. While it is established that ECs across different tissues exhibit heterogeneity in molecular signatures and regenerative capacities, the extent of proliferation heterogeneity among ECs within the same organ or tissue remains largely unexplored.

The miR172a-ERF416/413 module regulates soybean seed traits.

Soybean (Glycine max) provides vegetable oils and proteins for human consumption. Its production depends on seeds and other production-related agronomic traits. How the seed traits are regulated in soybean remains largely unclear.

YY1 regulates vascular resistance and blood pressure dynamics through epigenetic control of m6A RNA modifications in VSMCs.

Aims: Recent GWAS analysis has identified YY1 as a novel locus associated with blood pressure traits; however, whether YY1 directly controls vasoreactivity remains unknown. The principal function of vascular smooth muscle cells (VSMCs) is to contract, which is essential for regulating vascular tone, blood flow, and blood pressure. We hypothesized that YY1, a transcription factor, facilitates vascular function by epigenetically regulating gene expression in VSMCs.

Fragmentation Functions of Charged Hadrons at Next-to-Next-to-Leading Order and Constraints on the Proton Parton Distribution Functions.

We present the first global analysis of fragmentation functions for light charged hadrons (π^{±}, K^{±}) at full next-to-next-to-leading order in quantum chromodynamics, incorporating world data from both single-inclusive electron-positron annihilation and semi-inclusive deep-inelastic scattering. The collinear factorization has been tested with low-momentum-transfer data and has demonstrated success at high hadron momenta. Additionally, we study the impact of current global data on hadron production to the parton distribution functions (PDFs), and find they favor a reduced asymmetry in the strange (anti)quark PDFs, as compared to the asymmetry predicted by state-of-the-art PDFs derived from inclusive data.

Dissolved oxygen sensing characteristics in blood by hydrogel optical fiber.

In this paper, a hydrogel fiber optic sensing probe with a fluorescence indicator doped in the core is proposed for dissolved oxygen detection in blood. The hydrogel fiber was made by photocrosslinking with a core-cladding structure made from PEGDA precursor solutions with different concentrations. By changing the concentration of the fluorescent indicator doped in the core of the hydrogel optical fiber and changing the length of the fiber and the thickness of the cladding, the sensing performance of the fiber for dissolved oxygen was optimized, and it was found that the effect of photobleaching could be reduced appropriately by lowering the excitation power.

Antimalarial spirooxindole alkaloids with a rare 6/5/5/6/6 polycyclic skeleton from the fungus Penicillium citrinum YSC-1 isolated from a medicinal plant.

OSMAC (one strain many compounds) provides a convenient strategy to produce chemically diverse and novel natural products. In the study, the application of an OSMAC approach on a fungus Penicillium citrinum YSC-1 isolated from a medicinal plant Chloranthus japonicus, using different culture media, led to the isolation and identification of seven new spirooxindole alkaloids penicitrimicins A-G (1-7) with a rare 6/5/5/6/6 polycyclic skeleton, along with two known compounds (8-9). The new structures were characterized based on the comprehensive spectroscopic analyses, including 1D, 2D NMR and HRESIMS data.

Study on an interpretable prediction model for pile bearing capacity based on SHAP and BP neural networks.

To facilitate rapid and precise predictions of pile bearing capacity, a Back Propagation (BP) neural network model has been developed utilizing data sourced from existing literature. The model incorporates several input parameters, including pile length, pile diameter, average effective vertical stress, and undrained shear strength. To enhance the optimization of the BP neural network's hyperparameters, five distinct optimization algorithms were employed: the Sine Cosine Optimization Algorithm (SCA), Snake Optimization Algorithm (SO), Pelican Optimization Algorithm (POA), African Vulture Optimization Algorithm (AVOA), and Chameleon Optimization Algorithm (CSA).

Regulation of Na diffusion and retention in oral cavity by arabinogalactan-protein fraction from gum arabic and its mechanism of enhancing saltiness perception.

The growing health hazards of high-salt diets require effective salt reduction strategies in food industry. Gum arabic (GA), as saltiness enhancer, is composed of polysaccharides and glycoproteins. However, key fraction and its role in saltiness-enhancing remain underexplored.

Response of microbial communities and physical and chemical properties in inoculated soils with Schl. F. sp. to different root exudates resistant to fusarium wilt.

Wax gourd wilt (WGW) is a destructive soil-borne disease, and grafting pumpkin rootstocks offers effective and eco-friendly control. "Haizhan 1" pumpkin rootstock exhibits superior disease resistance compared to , with root exudates improving soil microecology. However, the specific root exudate components involved in wilt resistance and their effects on soil microbial diversity remain unclear.

Self-Discharging Mitigated Quantum Battery.

As a quantum thermodynamic device that utilizes quantum systems for energy storage and delivery, the quantum battery (QB) is expected to offer revolutionary advantages in terms of increasing the charging power and the extractable work by using quantum resources. However, the ubiquitous decoherence in the microscopic world inevitably forces the QB to spontaneously lose its stored energy. This is called the self-discharging of the QB and severely limits its realization.

Beta-Sitosterol Enhances Classical Swine Fever Virus Infection: Insights from RNA-Seq Analysis.

Beta-sitosterol (BS), a naturally occurring phytosterol abundant in plants, has been reported to exhibit diverse biological activities, including immunomodulatory and antiviral effects. Classical swine fever virus (CSFV), a member of the Pestivirus genus, remains a persistent threat to the swine industry worldwide, causing considerable economic damage. Our research found that BS significantly enhances the replication of both the CSFV-Shimen strain and the attenuated C-strain vaccine virus in PK-15 cells.