Follistatin-like 1 mitigates intermittent hypoxia-induced melanoma lung metastasis in mice.

Purpose: Intermittent hypoxia (IH) mimicking obstructive sleep apnea (OSA) has been confirmed to induce tumor lung metastasis via oxidative stress and inflammation responses. Follistatin-like 1 (Fstl1), as a matricellular protein, plays critical roles in inflammatory diseases and cancer. This study aimed to investigate the effect and mechanism of Fstl1 on OSA-IH-induced tumor lung metastasis.

Soil heterogeneity influence on the distribution of heavy metals in soil during acid rain infiltration: Experimental and numerical modeling.

Acid rain is a global environmental problem that mobilizes heavy metals in soils, while the distribution and geochemical fraction of heavy metals during acid rain infiltration in heterogeneous soils are still unclear. In this study, we performed column experiments to investigate the distribution and geochemical fraction of Cu, Pb, Ni and Cd in heterogeneously layered soils during continuous acid rain infiltration. Chloride ion used as a conservative tracer was found to be uniformly distributed during acid rain infiltration, showing insignificant preferential flow effects in the column.

Mesenchymal stem cell transplantation worsens intestinal inflammation and microenvironment in PI3Kγ-knockout mice.

Considering the possible interaction between mesenchymal stem cells (MSCs) and PI3Kγ-associated drugs, we evaluated the efficacy and action mechanism of MSCs in the treatment of colitis in PI3Kγ-/- mice. Trinitro-benzene-sulfonic acid enema was used to create a colitis model, and MSCs were transplanted through the caudal vein to treat colitis in wild-type and PI3Kγ-/- mice. We sequenced microbial 16S rRNA genes in the colonic mucosa of PI3Kγ-/- and wild-type mice and quantified colonic IgA, IL-2, IL-10, IL-17A, occludin, and serum IgA.

Sesquiterpenes from and Their Cytotoxic Activities.

is a well-known traditional Chinese medicine. Due to its highest antimalarial efficacy, China has a long history of cultivating , and it is used for "clearing heat and detoxicating". Several, studies have shown that the extract exerts cytotoxicity.

A Berberine Bridge Enzyme-Like Protein, , Confers Soybean's Coordinated Adaptation to Aluminum Toxicity and Phosphorus Deficiency.

Phosphorus (P) deficiency and aluminum (Al) toxicity often coexist and are two major limiting factors for crop production in acid soils. The purpose of this study was to characterize the function of , a berberine bridge enzyme-like protein-encoding gene, in soybean () adaptation to Al and low P stresses. Present quantitative real-time PCR (qRT-PCR) assays confirmed the phosphate (Pi)-starvation enhanced and Al-stress up-regulated expression pattern of in soybean roots.

Temperature drives the assembly of Bacillus community in mangrove ecosystem.

Mangroves are located at the interface of terrestrial and marine environments, and experience fluctuating conditions, creating a need to better explore the relative role of the bacterial community. Bacillus has been reported to be the dominant group in the mangrove ecosystem and plays a key role in maintaining the biodiversity and function of the mangrove ecosystem. However, studies on bacterial and Bacillus community across four seasons in the mangrove ecosystem are scarce.

PM2.5 promotes NSCLC carcinogenesis through translationally and transcriptionally activating DLAT-mediated glycolysis reprograming.

Background: Airborne fine particulate matter (PM2.5) has been associated with lung cancer development and progression in never smokers. However, the molecular mechanisms underlying PM2.

Effect of three tongue needles acupoints Lianquan (CV23) and Hegu (LI4) combined with swallowing training on the quality of life of laryngeal cancer patients with dysphagia after surgery.

Objective: To evaluate the effect of acupuncture therapies administered in combination with swallowing training on the quality of life of laryngeal cancer patients with dysphagia after surgery.

Methods: Seventy-one postoperative patients with laryngeal cancer participated in this study. The patients diagnosed with swallowing dysfunction by video fluoroscopic swallowing examination (VFSE) were randomly divided into experimental group (n = 36) and control group (n = 35).

Genome-Wide Identification and Characterization of Transcription Factor Gene Family Members Reveal Their Diverse Functions in Tea Plant ().

Golden2, ARR-B, Psr1 (GARP) proteins are plant-specific transcription factors that play vital and diverse roles in plants. However, systematic research on the gene family in plants, including tea plant (), is scarce. In this study, a total of 69 genes were identified and characterized from the tea plant genome based on the B-motif sequence signature.

Surface molecularly imprinted solid-phase extraction for the determination of vancomycin in plasma samples using HPLC-MS/MS.

Vancomycin is a glycopeptide antibiotic used to treat infections caused by Gram-positive bacteria. Due to the narrow therapeutic index of vancomycin, it is necessary to develop a sensitive and reliable analytical method to monitor the drug concentration in plasma. A novel method based on surface molecularly imprinted solid-phase extraction combined with liquid chromatography-tandem mass spectrometry for the determination of vancomycin in plasma sample was developed.

[Identification of a variant Bw37 allele of the ABO gene].

Objective: To explore the molecular basis for an individual with a rare variant of Bw37 phenotype.

Methods: Tube agglutination testing was used to determine the ABO blood groups. Genotyping were carried out using polymerase chain reaction-sequence specific primer (PCR-SSP) and direct sequencing for exons 6 and 7 of the ABO locus.

Spent LiFePO: An old but vigorous peroxymonosulfate activator for degradation of organic pollutants in water.

Iron-based catalysts have been demonstrated to activate peroxymonosulfate (PMS) to generate reactive radicals, which is however limited by their complex preparation process, high costs and inefficiency for practical applications. Herein we obtain spent LiFePO (SLFP), with powerful catalytic capacity by a simple one-step treatment of the retired LiFePO cathode material, for PMS activation to decontaminate organic pollutants. Lithium defects and oxygen vacancies in SLFP play critical roles for PMS utilization, further confirmed by density functional theory (DFT) calculations.

Imaging manifestations of idiopathic granulomatous lobular mastitis on cone-beam breast computed tomography.

Purpose: To evaluate the imaging manifestations of idiopathic granulomatous mastitis (IGM) on cone-beam breast computed tomography (CBBCT).

Methods: We retrospectively evaluated the clinical data of 22 female patients (mean age, 34.73 ± 10.

Collaborative Routing Optimization Model for Reverse Logistics of Construction and Demolition Waste from Sustainable Perspective.

The construction industry is developing rapidly along with the acceleration of urbanization but accompanied by an increased amount of construction and demolition waste (CDW). From the perspective of sustainability, the existing research has mainly focused on CDW treatment or landfill disposal, but the challenge of reverse logistics of CDW recycling that provides overall CDW route planning for multiple participants and coordinates the transportation process between multiple participants is still unclear. This paper develops an optimization model for multi-depot vehicle routing problems with time windows (MDVRPTW) for CDW transportation that is capable of coordinating involved CDW participants and suggesting a cost-effective, environment-friendly, and resource-saving transportation plan.

Cd(IO)(PO) and CdMg(IO)(PO): metal iodate-phosphates with large SHG responses and wide band gaps.

The first NLO-active metal iodate-phosphates, namely, Cd(IO)(PO) and CdMg(IO)(PO) (1 and 2), with three types of NLO groups, have been reported. 1 and 2 are isostructural and the structure of 1 features a 3D network formed by the Cd(IO)(PO) groups. 1 and 2 with strong SHG signals of 4 × and 3.

Preliminary risk assessment of regional industrial enterprise sites based on big data.

An accurate and inexpensive preliminary risk assessment of industrial enterprise sites at a regional scale is critical for environmental management. In this study, we propose a novel framework for the preliminary risk assessment of industrial enterprise sites in the Yangtze River Delta, which is one of the fastest economic development and most prominent contaminated regions in China. Based on source-pathway-receptors, this framework integrated text and spatial analyses and machine learning, and its feasibility was validated with 8848 positive and negative samples with a calibration and validation set ratio of 8:2.

Evansella halocellulosilytica sp. nov., an alkali-halotolerant and cellulose-dissolving bacterium isolated from bauxite residue.

An alkali and salt-tolerating strain FJAT-44876 was isolated from the bauxite residue sample. The 16S rRNA gene sequence and phylogenetic analysis suggest that strain FJAT-44876 was a member of the genus Evansella. It grew at 15-45 ℃ (optimum 20-25 ℃) and pH 6.

Characteristics of alveolar macrophages in bronchioalveolar lavage fluids from active tuberculosis patients identified by single-cell RNA sequencing.

Tuberculosis (TB), is an infectious disease caused by ( ), and presents with high morbidity and mortality. Alveolar macrophages play an important role in TB pathogenesis although there is heterogeneity and functional plasticity. This study aimed to show the characteristics of alveolar macrophages from bronchioalveolar lavage fluid (BALF) in active TB patients.

Hydrogen sulfide-induced oxidative stress mediated apoptosis via mitochondria pathway in embryo-larval stages of zebrafish.

Hydrogen sulfide (HS), a highly toxic gas, has become a polluting gas that cannot be ignored, while HS exposure results in acute or chronic poisoning or even death in humans or animals and plants, but the relevant mechanisms remain poorly understood. In this study, 9-day-old zebrafish larvae were exposed continuously to culture medium containing 30 μM survival rate was counted on HS, and our results indicated that HS exposure increased intracellular ROS, Ca, NO and MDA contents and decreased SOD activity, meaning that HS caused oxidative stress in embryo-larval stages of zebrafish. Furthermore, we found that transgenic zebrafish (cms Tg/+ AB) displayed a lower fluorescence intensity, and cytochrome c oxidase (COX) activity and JC-1 monomer fluorescence ratio increased under HS treatment conditions.

High glucose-induced ROS-accumulation in embryo-larval stages of zebrafish leads to mitochondria-mediated apoptosis.

In recent decades, diabetes mellitus has become a major chronic disease threatening human health worldwide, and the age of patients tends to be younger; however, the pathogenesis remains unclear, resulting in many difficulties in its treatment. As an ideal model animal, zebrafish can simulate the processes of human diabetes well. In this study, we successfully established a model of diabetic zebrafish larvae in a previous work.

Berberine induces non-small cell lung cancer apoptosis via the activation of the ROS/ASK1/JNK pathway.

Background: Non-small cell lung cancer (NSCLC) accounts for approximately 85% of all lung cancers. Berberine (BBR), an isoquinoline alkaloid, is commonly used in traditional Chinese medicine. Previous studies have shown that BBR has a potential anti-tumor effect.

and Antimalarial Screening and Validation Targeting Plasmepsin V.

Malaria chemotherapy is greatly threatened by the recent emergence and spread of resistance in the parasite against artemisinins and their partner drugs. Therefore, it is an urgent priority to develop new antimalarials. Plasmepsin V (PMV) is regarded as a superior drug target for its essential role in protein export.

Hypoxic mesenchymal stem cell-derived extracellular vesicles ameliorate renal fibrosis after ischemia-reperfusion injure by restoring CPT1A mediated fatty acid oxidation.

Background: Renal fibrosis is a common pathological process of chronic kidney diseases induced by multiple factors. Hypoxic pretreatment of mesenchymal stem cells can enhance the efficacy of secreted extracellular vesicles (MSC-EVs) on various diseases, but it is not clear whether they can better improve renal fibrosis. The latest research showed that recovery of fatty acid oxidation (FAO) can reduce renal fibrosis.

A novel tRNA-derived fragment AS-tDR-007333 promotes the malignancy of NSCLC via the HSPB1/MED29 and ELK4/MED29 axes.

Background: Transfer RNA-derived fragments (tRFs) are a new class of small non-coding RNAs. Recent studies suggest that tRFs participate in some pathological processes. However, the biological functions and mechanisms of tRFs in non-small cell lung cancer (NSCLC) are largely unknown.

Optimization of photo-biomodulation therapy for wound healing of diabetic foot ulcers and .

Unclear optical parameters make photo-biomodulation (PBM) difficult to implement in diabetic foot ulcer (DFU) clinically. Here, 12 wavelengths (400-900 nm) were used to conduct PBM to heal DFU wounds in vitro and in vivo. PBM at 10 mW/cm and 0.