Characterization of vB_SaRS_FS19-1, a newly isolated broad-host-range and virulent bacteriophage infecting Staphylococcus aureus and its application in foods.

The bacteriophage vB_SaRS_FS19-1, isolated from urban sewage, produced numerous clear plaques, about 1 to 2 mm in diameter, on the S. aureus JP19 bacterial lawn. A broad host range of the bacteriophage vB_SaRS_FS19-1 was found, and the lysis rate of S.

The effects of dietary energy level on the growth performance of yaks () were studied based on omics technique.

Introduction: The yak (Bos grunniens) is uniquely adapted to the Qinghai-Tibet Plateau, yet nutritional guidelines for yaks remain limited and often follow cattle standards. This study aimed to clarify dietary energy requirements to improve yak feeding strategies.

Methods: Fattening yaks were assigned to three diets with different net energy for gain (NEg): low (LE, 4.

A proactive cholinergic-like acidic fibroblast growth factor delivery system for efficient protein enrichment and native drug release in the central nervous system to promote repair of injured spinal cord.

Spinal cord injury (SCI) is a severe neurological trauma resulting in sensory deficits, impaired motor function, and compromised autonomic regulation. Acidic fibroblast growth factor (aFGF) with neuroprotective properties holds significant promise for promoting SCI repair. However, challenges of aFGF related to short blood circulation time, susceptibility to degradation, and severely restricted ability to cross blood-spinal cord barrier (BSCB) have hindered further exploration and utilization in SCI.

An antioxidative injectable chitosan hydrogel based on tannic acid modified dialdehyde polyurethane nano-crosslinker targeting RIPK1 to regulate neuroinflammation for amelioration of Parkinson's disease.

Parkinson's disease (PD) is one of the most common age-related neurodegenerative diseases. Currently, the treatment of PD is mainly based on surgery and medication, and there is a pressing necessity to develop a new generation of strategies incorporating biomaterials for treating PD. Herein, an antioxidative injectable chitosan hydrogel crosslinked with tannic acid-modified dialdehyde polyurethane nanoparticles (TA@DAP) to target RIPK1 is produced for ameliorating PD.

A cell-free SHED lysate-hydrogel system for oral ulcer healing with anti-inflammatory and pro-angiogenic effects.

Oral ulcer (OU) is one of the most common mucosal diseases, yet current drug treatments yield unsatisfactory outcomes. Persistent inflammatory responses and insufficient angiogenesis are the two major obstacles to OU healing. Recently, stem cell-based therapies, particularly mesenchymal stem cells (MSCs), have shown great regenerative potential through their anti-inflammatory and proangiogenic properties in OU treatment.

Genomic diversity and functional insights of carbapenem-resistant revealed by centroid coding sequences analysis.

is a primary bacterial pathogen responsible for various human infections with the emergence of carbapenem-resistant (CRKP) as a significant clinical challenge. Although previous studies have classified based on homology of conserved genes, the biological implications of these classifications remain unclear. In this study, we extracted 47,222 centroid coding sequences (CDSs), representative sequences selected from clusters of highly similar CDSs, from 3,592 complete genomes and analysed the genomic characteristics of 4,458 CRKP strains using these centroid CDSs.

Protective effects of curcumin on epileptic rodent models by alleviating oxidative stress and inflammation: a meta-analysis and mechanism exploration.

Objective: The purpose of this study is to systematically evaluate the therapeutic effect of curcumin on rodent epilepsy models through a meta-analysis of multiple animal experiments. It will also explore its potential mechanism of anti-oxidative stress and anti-inflammation to provide a theoretical basis for the application of curcumin in the clinical treatment of epilepsy.

Methods: A total of 23 eligible animal studies were identified by searching eight databases (up to March 2025), including PubMed, Embase, Web of Science, Cochrane Library, and CNKI, Wan Fang, VIP, CBM.

Protein β-O-glucosylation by Legionella LtpM through short consensus sequons G-T/S and S-G.

Unlike N-glycosylation, protein O-glycosylation often lacks a strict consensus sequon, making synthesis of homogeneous O-glycoproteins and site-specific engineering of O-glucosylation challenging. Here we identify Legionella effector LtpM as a versatile protein β-O-glucosyltransferase recognizing extremely short two-residue sequons G-T/S and S-G. X-ray crystallography, molecular simulation and biochemical studies together reveal a unique catalytic mechanism: four residues of LtpM (F166, Q167, W228 and K225) serve as 'gatekeepers' above the binding pocket of the uridine diphosphate (UDP)-glucose sugar donor to form a narrow clamp for the substrate proteins, limiting the residue adjacent to serine or threonine to be exclusively glycine.

Characterizing Kupffer Cell Production of CD5 Antigen-Like and Its Function on Regulating Migration of Natural Killer T Cells.

CD5 antigen-like (CD5L) is a multifunctional glycoprotein characterized for its role in the lipid metabolism, particularly within macrophages. In the liver, CD5L strongly correlates with liver injury. This study explored the role of CD5L on liver lipid accumulation and inflammatory response.

Bismuth-Doped AgTe Colloidal Quantum Dots for High-Performance Shortwave-Infrared Photodetectors.

Recently, silver telluride (AgTe) colloidal quantum dots (QDs) have garnered significant attention in short-wave-infrared (SWIR) photodetection owing to their strong SWIR responsivity and environmental benignity. However, the SWIR AgTe QDs often suffer from their high defect density, leading to an unsatisfactory utilization efficiency of the photogenerated charge carriers. To address this challenge, here, we have developed bismuth-doped AgTe (Bi:AgTe) QDs via a hot-injection doping method.

Parametric analysis of passive ultra-low energy building envelope performance in existing residential buildings.

With the proposed goals of "energy conservation, carbon reduction, and green development," green and low-carbon lifestyles have gained significant attention. As a major sector of energy consumption, residential buildings face common issues such as high energy consumption, poor comfort, and aging facilities. Taking a residential building in the West Coast New Area of Qingdao, Shandong Province as a case study, this research employs field surveys and DesignBuilder software simulations to analyze the performance of building envelopes and interior walls in non-heated spaces.

[Results of screening and prenatal diagnosis for 71 fetuses with high risk for trisomy/monosomy 13 syndrome by non-invasive prenatal screening].

Objective: To analyze the clinical data and results of prenatal diagnosis for fetuses with high-risk for trisomy/monosomy 13 by non-invasive prenatal testing (NIPT).

Methods: Clinical data of pregnant women with fetus at a high risk for trisomy/monosomy 13 by NIPT at the First Affiliated Hospital of Zhengzhou University from May 2016 to May 2024 were reviewed, and relevant data such as Z-score, positive predictive value (PPV) and fetal fraction (FF) were analyzed to assess the correlation between them. This study was approved by the Ethics Committee of the Hospital (No.

A crystalline-amorphous CoS/CoOOH heterostructure enables efficient and stable SOR-coupled hydrogen evolution.

The development of efficient and durable electrocatalysts for SOR-coupled HER systems is critical for advancing sustainable hydrogen production. Herein, we present a crystalline-amorphous CoS/CoOOH heterostructure anchored on nickel foam (NF) synthesized a hydrogen peroxide-assisted hydrothermal method, where lattice-ordered CoS is partially oxidized and reconstructed into amorphous CoOOH. This crystalline-amorphous heterostructure catalytic system enhances charge transfer efficiency, reducing the SOR onset potential from 1.

Metabolic reprogramming in hepatocellular carcinoma: an integrated omics study of lipid pathways and their diagnostic potential.

Metabolic reprogramming is an important cancer hallmark. Recent studies have indicated that lipid metabolic reprogramming play a potential role in the development of hepatocellular carcinoma (HCC). However, the underlying mechanisms remain incompletely understood.

Targeting c-MYC has a key role in hepatocellular carcinoma therapy.

Hepatocellular carcinoma (HCC) is a top cause of cancer-associated mortality worldwide, with limited effective treatment options. The oncogenic transcription factor c-MYC plays a pivotal role in HCC pathogenesis by regulating key cellular processes, including proliferation, metabolism, and apoptosis. Impaired c-MYC regulation strongly correlates with aberrant activation of multiple signaling pathways, such as PI3K/Akt/mTOR, Wnt/β-catenin, and MAPK/ERK, which collectively drive tumor progression.

Single-Cell and Spatial Transcriptomic Profiling of Penile Squamous Cell Carcinoma Reveals Dynamics of Tumor Differentiation and Immune Microenvironment.

Penile squamous cell carcinoma (PSCC) is a highly aggressive malignancy without effective treatment due to limited knowledge of its development and tumor microenvironment (TME). In this study, single-nucleus RNA sequencing (snRNA-seq) and high-resolution spatial transcriptomics are employed to comprehensively investigate the development trajectories and the TME. The results revealed that PSCC cells mimicked the differentiation and tissue organization of normal penile epithelium, independent of the human papillomavirus (HPV) infection status.

Discovery of Coumarin-7-/-Acethydrazide Derivatives as Novel Antifungal and Herbicidal Agents.

Natural products (NPs) have attracted increasing attention due to their structural diversity, abundance, and low environmental impact, making them valuable for novel fungicide development. In this study, a series of coumarin-7-/ acethydrazide derivatives were synthesized by incorporating acetic acid moieties into the coumarin scaffold. The in vitro antifungal activities of these compounds were evaluated against six common plant pathogenic fungi.

Discovery of novel 3-phenylhydrazone coumarin derivatives as potential antifungal agents against phytopathogenic fungi.

Plant fungal diseases, such as rice sheath blight caused by Rhizoctonia solani, significantly impact crop yield and quality. To discover novel fungicides, 39 novel 3-phenylhydrazone coumarin compounds were synthesized using natural product modeling and active substructure merging strategies. Their bioactivity was tested against six plant pathogens, including Botrytis cinerea, Alternaria solani, Fusarium graminearum, Rhizoctonia solani, Colletotrichum orbiculare, and Alternaria alternata.

Polydatin inhibits the stemness and angiogenesis of gastric cancer cells by targeting down-regulation of HDAC7.

Gastric cancer (GC) is a prevalent malignancy of gastrointestinal tract with a high incidence worldwide. Polydatin, a bioactive compound in Polygonum cuspidatum, possesses antitumor effects. We aimed to study the role of polydatin in GC and its possible mechanism.

Hsa-circVIM regulates breast cancer tumor progression and tamoxifen sensitivity by sponging miR-1294 in hormone receptor-positive breast cancer cells.

Background: The progression and metastasis of breast cancer patients are regulated by genetics and epigenetics. Circular RNA (circRNA) plays a pivotal role in modulating the advancement of tumors. The study aimed to explore the clinical performance and regulatory role of hsa-circVIM in breast cancer and its modulatory effect on tamoxifen resistance in hormone receptor (HR) positive breast cancer cells.

Design, Synthesis, Antifungal Evaluation, and Action Mechanism of Novel l-Carvone-Based Derivatives as Potential Succinate Dehydrogenase Inhibitors.

Natural products serve as a crucial source of compounds for developing novel agricultural antifungals. In this work, we utilized the natural product l-carvone as a molecular scaffold to design and synthesize three series of novel l-carvone-based derivatives. The bioassay results indicated that most target compounds exhibited remarkable antifungal activity.

Glucose intercalation-induced 1T-G-MoS hybrids for high-performance rechargeable aqueous zinc-ion batteries.

Owing to 1T phase MoS (1T-MoS) possessing higher electron conductivity than 2H phase MoS (2H-MoS), 1T-MoS is considered as a more promising electrode material for aqueous zinc-ion batteries (AZIBs). Herein, a glucose intercalation-assisted induction strategy is employed to prepare stable 1T-rich MoS hybrids (1T-G-MoS) for boosting zinc storage performance. The glucose is intercalated into MoS through a one-step facile hydrothermal method, which expands the interlayer spacing and improves the hydrophilicity, facilitating reversible insertion/extraction kinetics of Zn ions.

Dipole-Dipole Interaction-Induced Direct Self-Assembly of AgS Quantum Dots into Supercrystals in Solution.

Long-range ordered supercrystals (SCs) built up by colloidal nanocrystals (NCs) represent a class of novel metamaterials with unique collective properties. While great attention has been paid to the ligand-controlled assembly of NCs, the contribution of the inorganic core is considered limited because of the weak core-core interactions. Here, we report the spontaneous assembly of AgS quantum dots (QDs) into three-dimensional SCs in solution, driven by pronounced dipole-dipole interactions.

Sulfur defect engineering controls LiS crystal orientation towards dendrite-free lithium metal batteries.

Controlling nucleation and growth of Li is crucial to avoid dendrite formation for practical applications of lithium metal batteries. LiS has been exemplified to promote Li transport, but its crystal orientation significantly influences the Li deposition behaviors. Here, we investigate the interactions between Li and various surface structures of LiS, and reveal that the LiS(111) plane exhibits the highest Li affinity and the lowest diffusion barrier, leading to dense Li deposition.