[Source Analysis and Ecological Risk Assessment of Heavy Metals in Soil of the Yifeng County， Jiangxi Province Based on APCS-MLR Receptor Model].

The enrichment of heavy metals in soil is a consequence of both natural processes and anthropogenic activities. Conducting source analysis and ecological risk assessment of heavy metals in soil is a necessary approach for serving the prevention and control of soil heavy metal pollution. To identify the sources and ecological risk characteristics of heavy metals in the soil of Yifeng County， Jiangxi Province， surface soil samples were collected， and the heavy metal content and pH value were analyzed.

Bimodal onset and pan-cancer uniformity of immune-mediated liver injury: a retrospective cohort study.

Background: Immune-mediated liver injury (IMLI) is a critical adverse event in patients treated with PD-1/PD-L1 inhibitors. The study aims to characterize the clinical heterogeneity, temporal dynamics, and immunological drivers of PD-1/PD-L1 inhibitor-associated IMLI and optimize surveillance and management strategies.

Methods: We retrospectively recruited 373 IMLI patients.

Spatial heterogeneity of soil Cd and Pb driven by combined effects of pedogenesis and anthropogenic activities: A case study in the mountainous and hilly region of Fujian Province, China.

Soil is a vital and fragile natural resource in the Earth's surface system, with the accumulation of potentially toxic elements (PTEs), such as Cd and Pb, drawing global environmental concern. Soil convergent evolution may lead to the homogenization of major elemental compositions; however, the main mechanisms controlling the distributions of PTEs in soils remain insufficient. Furthermore, the combined effects of pedogenesis and anthropogenic activities on the geochemical behaviors of PTEs are still poorly understood.

Landscapes of immunoglobulin heavy-chain gene repertoire and its cytogenetic abnormalities in Chinese patients with multiple myeloma.

Multiple myeloma (MM) is a heterogeneous disease, the full understanding of whose pathogenesis remains elusive. While B-cell receptors are known to play a pivotal role in myeloma pathogenesis, the characterization of immunoglobulin heavy-chain (IGH) gene repertoire and their clinical significance in Chinese patients has not been fully explored. In this study, we analyzed the profiling of clonal IGH gene rearrangements via NGS assay, and its cytogenetic abnormalities by FISH in a cohort of 301 Chinese patients with newly diagnosed MM.

Peanut 9--epoxycarotenoid Dioxygenase Enhances Salt and Drought Stress Tolerance by Regulating ROS Homeostasis.

Peanut ( L.), a vital oilseed and cash crop, faces yield limitations due to abiotic stresses. The 9--epoxycarotenoid dioxygenase (NCED) enzyme, a key enzyme in abscisic acid (ABA) biosynthesis regulating plant development and stress responses, remains mechanistically uncharacterized in peanut abiotic stress tolerance.

Auxiliary Electrode Tunes Wet-Electrospun Bundle Stiffness to Modulate Cell Phenotype.

The stiffness of tissue-engineered scaffolds significantly influences cell behaviour and phenotype. However, current approaches to tuning stiffness often introduce unintended variations and compromise topographical consistency. In this study, an innovative wet-electrospinning set-up, incorporating a positively charged auxiliary electrode was developed to fabricate bundles with adjustable stiffness.

Identification, Pathogenicity, and Reverse Genetics System Construction of a Pseudorabies Virus Isolate from Pigs in China.

Pseudorabies virus (PRV) is a highly contagious pathogen in swine that can cross species barriers and infect other mammals, including humans. Given the potential for interspecies transmission and its threat to public health, understanding the molecular biology of PRV strains is essential for developing effective control measures and preparing for future pandemics. In this study, a novel PRV strain, PRV-HL-2021, was isolated from an outbreak in Heilongjiang Province, China.

Solar-Driven Dual-functional Adsorvaporator Enabling Efficient Lithium Concentration and Freshwater Generation with Life Cycle Assessment Evaluation.

Solar vapor generation (SVG) has emerged as a sustainable and energy-efficient strategy for freshwater production and mineral extraction from brines. Here, the intrinsic water-salt ion transport characteristics of SVG are harnessed to develop a dual-functional "adsorvaporator"-an upcycled hybrid platform that seamlessly integrates simultaneous lithium adsorption/concentration and solar vapor generation. This adsorvaporator is constructed from a molybdenum disulfide (MoS) photothermal components- and HMnO (HMO) ion sieves- embedded acrylamide hydrogel (MHA) with anti-salt and saturated lithium adsorption capabilities, which can enable synergistic lithium recovery and high-performance water evaporation.

Cell cycle dynamics analysis of drug-tolerant persister cells in non-small cell lung cancer.

Molecular-targeted therapy remains effective in cancer treatment; however, drug resistance inevitably arises after prolonged administration for several years. Drug-tolerant persister cells (DTPs), which are subpopulations of cells derived from drug-sensitive cells that develop tolerance after short-term exposure (lasting days or weeks) to drugs, have gained substantial attention. However, the specific characteristics of DTPs remain unclear as they encompass a diverse cell population, although they generally exhibit low proliferative potential.

Effect of climate on traits of dominant and rare tree species in the world's forests.

Species' traits and environmental conditions determine the abundance of tree species across the globe. The extent to which traits of dominant and rare tree species differ remains untested across a broad environmental range, limiting our understanding of how species traits and the environment shape forest functional composition. We use a global dataset of tree composition of >22,000 forest plots and 11 traits of 1663 tree species to ask how locally dominant and rare species differ in their trait values, and how these differences are driven by climatic gradients in temperature and water availability in forest biomes across the globe.

Temporal and spatial distribution of chlorinated hydrocarbons in surface sediments of the Baltic Sea and Skagerrak over the past 30 years.

The Baltic Sea has been heavily impacted by harmful and persistent organic pollutants (POPs), including polychlorinated biphenyls (PCBs), dichlorodiphenyltrichloroethanes (DDTs), and hexachlorobenzene (HCB), with peak emissions occurring between 1960 and 1980. Despite restrictions on their production and use implemented over 40 years ago due to concerns about their threats to marine wildlife and consumers, POPs remain at elevated concentrations. Their persistence in the environment underscores the need for long-term monitoring.

Genetic analysis and fine mapping reveal that AhRt3, which encodes an anthocyanin reductase, is responsible for red testa in cultivated peanuts.

AhRt3, which governs the red testa of peanut, was narrowed down to a 125.30 kb region, and one gene encoding anthocyanin reductase was identified as the putative candidate gene. Testa color is a special characteristic of peanuts (Arachis hypogaea L.

Radio frequency switching devices based on two-dimensional materials for high-speed communication applications.

Two-dimensional (2D) materials, with their atomic-scale thickness, high carrier mobility, tunable wide bandgap, and excellent electrical and mechanical properties, have demonstrated vast application prospects in research on radio frequency (RF) switch devices. This review summarizes the recent advances in 2D materials for RF switch applications, focusing on the performance and mechanisms of 2D material-based RF switch devices at high frequencies, wide bandwidths, and high transmission rates. The analysis includes the design and optimization of devices based on graphene, transition metal dichalcogenides, hexagonal boron nitride, and their heterojunctions.

Label-free quantification of antibody-oligonucleotide conjugates with varying stoichiometries using capillary zone electrophoresis.

We report a label-free capillary zone electrophoresis platform enabling quantitative analysis of antibody-oligonucleotide conjugate (AOC) heterogeneity with single-stranded oligonucleotide (21 nt) resolution. This methodology resolves free antibodies, oligonucleotides, and AOC subpopulations while precisely evaluating conjugation efficiency and oligonucleotide-to-antibody ratio (OAR) heterogeneity across three synthetic strategies.

The Development of a Coaxial Electrospinning Formula Using Fish Gelatin/PBS as the Core for Structurally Intact Liposome Loading and Release.

In electrospun scaffolds, coaxial electrospinning is gaining increased attention due to its potential for biocomponent encapsulation and controlled delivery. However, the encapsulation of biocomponents, such as liposomes, remains challenging because of their low stability in commonly used electrospinning solvents. This study, therefore, aims to develop a novel coaxial electrospinning formulation for crafting a liposome-encapsulated, rapid-release coaxial fiber.

Trigeminal ganglion electrical stimulation relieves refractory trigeminal herpes zoster and prevents postherpetic neuralgia: a case report.

Introduction: Herpes zoster is an acute condition caused by the reactivation of varicella zoster virus, often affecting the skin and mucosa. When varicella zoster virus invades the trigeminal ganglion, it can lead to trigeminal herpes zoster, with postherpetic neuralgia as the most common complication. Postherpetic neuralgia, characterized by persistent pain for over 3 months after skin lesions heal, significantly impacts quality of life, including sleep, mood, and social interactions.

Single-Molecule Mapping Landscape of Multivalent Antibody-DNA Framework Conjugates.

Patterned assembly of multivalent antibody complexes using DNA nanostructure templates holds the potential for advancing studies of cellular signaling and smart theranostic applications. However, evaluating the heterogeneity in protein conjugation efficiency at distinct sites on DNA templates remains challenging. Here, we utilize atomic force microscopy to measure the coupling of antibodies at various positions on two-dimensional rectangular DNA origami frameworks at the single-molecule level, generating spatial maps of antibody binding efficiencies across the structures.

Functionalization of Nucleic Acid Molecular Machines under Physiological Conditions: A Review.

In-situ fabrication of nucleic acid molecular machines in biological environments is desirable for smart theranostic applications. However, given the complex nature of biological environments, the integration of multiple functional modules into a coordinated machine remains challenging. Recent advances in nucleic acid nanotechnology offer solutions to these challenges.

Isolation of endophytes from Taxus cuspidata cv. Nana, preparation of extracellular polysaccharides and study on their functional properties.

In this study, the target strain Methylorubrum sp. Y19 was isolated and purified from Taxus cuspidata cv. Nana, leading to the preparation of extracellular polysaccharides (EPS) EPS.

Predicting the Cell-level PD-L1 Expression Status from H&E-stained Histopathological Slide.

TPS (Tumor Proportion Score) serves as a crucial indicator to determine the eligibility of cancer patients for PD-L1 immunohistochemistry (IHC) treatment. In clinical practice, pathologists assess TPS primarily using IHC histopathological slides, which is time-consuming and incurs high treatment costs. In this paper, we introduce a novel approach for predicting PD-L1 positive cells using cost-effective and widely accessible Hematoxylin and Eosin (H&E) stained slides.

Ensartinib for advanced or metastatic non-small-cell lung cancer with exon 14 skipping mutations (EMBRACE): a multi-center, single-arm, phase 2 trial.

Background: exon14 skipping mutations (ex14) is an established actionable driver oncogene of non-small-cell lung cancer (NSCLC). While ensartinib is a known second-generation tyrosine kinase inhibitor with primary activity against translocation, it is also classified as a type Ia MET inhibitor. We have previously shown anti-tumor activity against ex14 positive NSCLC both and .

Designing a Patient Preference Study on Subcutaneous Medical Devices: Incorporating Health Authority Scientific Advice and Patient Perspectives.

This paper describes the planning of a patient preference study for evaluating device features for the subcutaneous (SC) delivery of high dose/large volume (e.g., > 2 ml) of drugs.

Recent Advances in DNA-Templated Protein Patterning.

In recent decades, the advancement of DNA nanotechnology enables precise nanoscale organization of diverse functional materials with DNA templates. Particularly, a variety of DNA-templated protein patterns are constructed as powerful tools for programming biomimetic protein complexes. In this review, recent progress in DNA-templated protein patterning, including cutting-edge methods for arranging proteins with DNA templates, and protein patterns across varying dimensions are briefly summarized.

The Influence of Mg Doping in α-AlO Crystals Investigated with First-Principles Calculations and Experiment.

The influence of Mg doping in α-AlO crystals is investigated in this article by first-principles calculations and formation energies, density of states, and computed absorption spectra. Three models related to Mg substituting for Al doping structures were constructed, as well as spinel structure models with varying aluminum-magnesium ratios. The formation energy calculations confirmed the rationality of the MgV model, which means that Mg substitutional doping incorporating oxygen vacancies is most likely to form in crystals.