SERS spectrum of saliva combined with machine learning algorithm for early diagnosis and monitoring of periodontal disease.

To achieve non-invasive early diagnosis and severity monitoring of periodontal disease, this study employed silver nanoparticles as a surface-enhanced Raman Scattering (SERS) substrate for the detection and analysis of salivary SERS spectra from a control group (periodontal health group) and groups with periodontal disease of varying severities (including gingivitis, as well as mild, moderate, and severe periodontitis). The results demonstrated significant differences in salivary SERS spectra between the control group and the gingivitis group, between the control group and the mild periodontitis group, and between the control group and groups with periodontal disease of different severities. Subsequently, based on the dual screening criteria of Variable Importance in Projection (VIP) ≥ 1 and P < 0.

Sirt4 Deficiency Promotes Cardiomyocyte Proliferation and Cardiac Repair.

The mammalian heart exhibits transient but remarkable regenerative capacity during the early postnatal period, after which most cardiomyocytes exit the cell cycle. While the sirtuin family is well-established as regulators of cell cycle progression, its specific role in cardiomyocyte proliferation and cardiac regeneration remains unclear. In this study, we found that Sirt4 expression increased during postnatal heart development.

Construction of a Prediction Model for Sleep Quality in Embryo Repeated Implantation Failure Patients Undergoing Assisted Reproductive Technology Based on Machine Learning: A Single-Center Retrospective Study.

Objective: Constructing a predictive model for sleep quality in embryo Repeated Implantation Failure(RIF) patients using multiple machine learning algorithms, verifying its performance, and selecting the optimal model.

Methods: Retrospective collection of clinical data from RIF patients who underwent assisted reproductive technology at the Reproductive Medicine Center of Tongji University Affiliated Obstetrics and Gynecology Hospital from January 2022 to June 2022, divided into a training set and a validation set in an 8:2 ratio. Use Lasso regression to screen variables and construct a risk prediction model using six machine learning algorithms.

Matrix metalloproteinase 9 deficiency promotes endogenous cardiomyocyte proliferation.

Matrix metalloproteinase 9 (MMP9) is known to modulate cardiac remodeling after myocardial infarction, but its role in cardiomyocyte proliferation remains unclear. Here, we showed that MMP9 deficiency enhanced neonatal cardiomyocyte proliferation and mononucleation following apical resection. Integrated transcriptomic and proteomic analyses revealed that MMP9 knockout induces a metabolic shift from oxidative phosphorylation to glycolysis in injured neonatal hearts, coinciding with upregulation of acyl-CoA thioesterase 1 (ACOT1).

Mydgf Enhances Cardiac Angiogenesis by Upregulating FGF1.

Following myocardial infarction (MI), endothelial cell proliferation drives neovascularization to support cardiac regeneration. While myeloid-derived growth factor (Mydgf) is known to promote cardiac repair post-injury, yet its mechanism in angiogenesis remained unclear. In this study, we found that myeloid-derived growth factor knockout (Mydgf-KO) reduced endothelial cell proliferation during early postnatal cardiac angiogenesis and impaired neovascularization post-MI, leading to exacerbated cardiac dysfunction.

Theoretical Prediction of a Novel Phase TiB Monolayer as an Outstanding Electrode Material for Lithium/Sodium-Ion Batteries.

Transition metal borides (MBenes) have provoked widespread interest in the field of power storage due to their excellent electrochemical performance. Using structural prediction software and first-principles calculations, a two-dimensional TiB phase has been predicted; it possesses favorable structural stability and metal-like electronic characteristics. The TiB monolayer is suitable as an electrode material for lithium/sodium storage due to its excellent ion migration ability, moderate open circuit voltage, and high storage capacity.

Exquisite hybridized electrocatalyst with moderate selenium element integration for reliable lithium-sulfur batteries.

Effective immobilization and catalytic conversion of lithium polysulfides (LiPSs) are crucial for enhancing lithium-sulfur (Li-S) battery performance. To this end, a hybrid CoSe-CoO-Se@CNTs electrocatalyst was rationally designed, wherein CoO and CoSe synergistically promote LiPS adsorption and catalytic transformation, while CNTs provide a conductive framework to enhance sulfur utilization. Notably, the often-overlooked role of selenium was elucidated via in situ X-ray diffraction pattern revealing its active participation in charge-discharge reactions, which contributes to the stable cycling performance of Li-S batteries.

Foxk1 and Foxk2 promote cardiomyocyte proliferation and heart regeneration.

Promoting endogenous cardiomyocyte proliferation is a promising strategy for cardiac repair. Identifying key factors that regulate cardiomyocyte proliferation can advance the development of novel therapies for heart regeneration. Here, we identify Foxk1 and Foxk2 as key regulators of cardiomyocyte proliferation, whose expression declines during postnatal heart development.

Fluorescence in situ hybridization protocol for cardiomyocytes.

Measuring cardiomyocyte nuclear ploidy is crucial for evaluating broader aspects of cardiac development, function, and disease progression. Fluorescence in situ hybridization (FISH) remains the gold standard for ploidy identification; however, its application in cardiomyocytes is hindered by their unique cellular complexities. Here, we describe a detailed cardiomyocyte-specific FISH (cardioFISH) protocol.

The Correlation Between Symptom Clusters and Quality of Life in Patients with Systemic Lupus Erythematosus: A Cross-Sectional Study.

Background: Systemic lupus erythematosus (SLE) is characterized by a range of symptoms that often cluster together, impacting the quality of life (QoL) of affected individuals.

Objective: To delineate the composition of symptom clusters in patients with SLE and analyze their correlation with QoL, thus providing a basis for symptom management.

Methods: Using convenience sampling, 201 patients were recruited.

The Application Effect of Fine Management Combined with Man-Machine Fixation Mode in Reducing the Attrition Rate of Laparoscopic Instruments-A Non-Randomized, Concurrent Controlled Study.

Purpose: To explore the effect of fine management combined with man-machine fixation mode in reducing the attrition rate of laparoscopic instruments.

Methods: This is a non-randomized, concurrent controlled study, from December 1, 2023, to May 1, 2024, the use of 4 laparoscopic instruments in the operating room which belongs to shanghai first maternity and infant hospital was analyzed. The instruments were divided into two groups, and the control group was managed by conventional methods.

Electrophilic aromatic substitution of electron-rich arenes with -fluorobenzenesulfonimide (NFSI) as an electrophile.

An efficient amidation of electron-rich arenes using NFSI as a nitrogen source has been successfully disclosed. This amidation process can be easily conducted at elevated temperatures, without the need for catalysts or additives. A wide range of arenes substituted with hydroxy, alkoxy, or carbonyl groups were found to be compatible, yielding the desired amination products.

The functional subclasses of AT1 receptor autoantibody in patients with coronary heart disease.

Recently, the identification of autoantibodies (AT1-AA) targeting the second extracellular loop of angiotensin II type 1 receptor (AT1R-ECII) in patients with coronary heart disease (CHD) offers a novel perspective on the interplay between immunity and cardiovascular disease. However, much remains unknown regarding the functional diversity of AT1-AA. In this study, we measured the levels of AT1-AA in the sera of 306 CHD patients and purified AT1-AA from patient's sera (n = 127).

[Loss of Myeloid-Derived Growth Factor Leads to Increased Fibrosis in Mice After Myocardial Infarction].

Objective: To investigate the effect of the loss of myeloid-derived growth factor (Mydgf) on the transformation of cardiac fibroblasts into myofibroblasts after myocardial infarction (MI).

Methods: Two adult mouse groups, including a wild-type (WT) group and another group with knockout (-KO), were examined in the study. The mice in these two groups were tested for their cardiac function by measuring left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) (=10).

IL-32 regulates trophoblast invasion through miR-205-NFκB-MMP2/9 axis contributing to the pregnancy-induced hypertension†.

Interleukin-32 is a species-specific cytokine that plays an important role in inflammation, cancer, and other diseases; however, its role in reproductive and pregnancy-related diseases remains unknown. This study aimed to investigate the role of interleukin-32 in reproductive and pregnancy-related diseases. Placental tissues from patients with pregnancy-induced hypertension, healthy pregnant women, and trophoblast lines were analysed.

Bibliometric Research on Surgical Scheduling Management from the Perspective of Web of Science.

Objective: Reasonable surgical scheduling management is crucial to optimize the utilization rate of operating room. This study aims to understand the context, frontier and hot spots of surgical scheduling management research, in order to provide reference for surgical scheduling optimization.

Methods: Literature on operation scheduling management collected in Web of Science core collection database was searched from the database establishment to June 21, 2023.

Comparative analysis of codon usage patterns in the chloroplast genomes of nine forage legumes.

Unlabelled: is one of the three largest families of angiosperms after and . It is widely distributed and grows in a variety of environments, including plains, mountains, deserts, forests, grasslands, and even waters where almost all legumes can be found. It is one of the most important sources of starch, protein and oil in the food of mankind and also an important source of high-quality forage material for animals, which has important economic significance.

Identification of Key Ubiquitination-Related Genes and Their Association with Immune Infiltration in Osteoarthritis Based on the mRNA-miRNA Network.

Osteoarthritis (OA) is a prevalent degenerative joint disease that is closely associated with functions of ubiquitination and immune cells, yet the mechanism remains ambiguous. This study aimed to find core ubiquitination-related genes and their correlative immune infiltration in OA using weighted gene co-expression network analysis (WGCNA). The ubiquitination-related genes, datasets GSE55235 and GSE143514 were obtained from open databases.

Effect of In Situ NbC-CrC@graphene/Fe Nanocomposite Inoculant Modification and Refinement on the Microstructure and Properties of W18Cr4V High-Speed Steel.

A novel graphene-coated nanocrystalline ceramic particle, iron-based composite inoculant was developed in this study to optimize the as-cast microstructure and mechanical properties of W18Cr4V high-speed steel (HSS). The effects of the composite inoculant on the microstructure, crystal structure, and mechanical properties of HSS were analyzed using transmission electron microscopy, scanning electron microscopy, energy dispersive spectroscopy, and X-ray diffraction. The (002-) and (020) crystal planes of the FeC and CrC phases, respectively, were collinear at two points in the reciprocal space, indicating a coherent relationship between the FeC and CrC phases in the tempered modified HSS.

A tungsten phosphide cocatalyst enhanced bismuth tungstate photoanode with the robust built-in electric field towards highly efficient photoelectrochemical water splitting.

The use of low-cost and effective cocatalyst is a potential strategy to optimize the effectiveness of photoelectrochemical (PEC) water splitting. In this study, tungsten phosphide (WP) is introduced as a remarkably active cocatalyst to enhance the PEC efficiency of a BiWO photoanode. The onset potential of BiWO/WP demonstrates a negative shift, while the photocurrent density demonstrates a significant 5.

A Study on the Psychological Experience and Influential Factors of Pregnant Women Who Decided Elective Caesarean Section After Public Health Emergencies - A Cross-Sectional Survey.

Background: The COVID-19 pandemic used to be a major public health emergency which affected people worldwide, and it affected individuals' body, mood, work and lifestyle to some extent. The pregnant woman affected by the unstable hormone will be more sensitive than normal ones. Long-term depression and anxiety could feedback on their body and lead to a host of pregnancy complications.

Knockdown of angiopoietin-like 4 suppresses sepsis-induced acute lung injury by blocking the NF-κB pathway activation and hindering macrophage M1 polarization and pyroptosis.

Objective: Sepsis-induced acute lung injury (ALI) is a life-threatening disease. Macrophage pyroptosis has been reported to exert function in ALI. We aimed to investigate the mechanisms of ANGPTL4-mediated cell pyroptosis in sepsis-induced ALI, thus providing new insights into the pathogenesis and prevention and treatment measures of sepsis-induced ALI.

Interaction of air pollution and meteorological factors on IVF outcomes: A multicenter study in China.

Background: Previous studies revealed associations between air-pollutant exposure and in vitro fertilization (IVF) outcomes. However, modification effects of air pollution on IVF outcomes by meteorological conditions remain elusive.

Methods: This multicenter retrospective cohort study included 15,217 women from five northern Chinese cities during 2015-2020.

EZH2 controls epicardial cell migration during heart development.

Enhancer of zeste homolog 2 (EZH2) is an important transcriptional regulator in development that catalyzes H3K27me3. The role of EZH2 in epicardial development is still unknown. In this study, we show that EZH2 is expressed in epicardial cells during both human and mouse heart development.