Distribution of glucosinolates and antioxidants in different leaf-layers of Kale ( var. DC.).

. Kale ( var. DC.

E3 ligase RAD18 targets phosphorylated IRF3 to terminate IFNB1 transcription.

The transcription factor interferon regulatory factor 3 (IRF3) initiates type I interferon transcription, which is required for host defense. Here, we identify RAD18 as a central E3 ubiquitin ligase that selectively targets phosphorylated IRF3 (p-IRF3) for autophagic degradation. RAD18 specifically promotes the dissociation of p-IRF3 from the IFNB promoter and in turn terminates its transcriptional activity.

Consumption of fermented Cd and Pb contaminated wheat flour increases potential health risk.

Fermentation can increase the availability of heavy metals in wheat flour, however, its effect on the health risk of heavy metal contaminated wheat flour has not been fully investigated. A contaminated wheat flour (Pb = 0.26, Cd = 0.

Multifunctional miRNA delivery systems provide synergistic neuroprotection against cerebral ischemia reperfusion injury.

Effectively alleviating cerebral ischemia-reperfusion (I/R) injury is challenging despite the medical advances. Prompt restoration of blood flow after ischemic stroke causes secondary damage to the brain tissues, triggers neuroinflammation and overproduces reactive oxygen species (ROS). MiRNA regulates the genes involved in neuron apoptosis and neuroinflammation, thus exhibiting potential in ameliorating cerebral I/R injury.

A comprehensive review on the phytochemistry, pharmacological activities, geographical distribution and genetic relationships of Salvia yunnanensis.

Salvia yunnanensis C. H. Wright (S.

Evaluation of the analgesic efficacy of ultrasound-guided quadratus lumborum block for laparoscopic renal surgery: a randomized controlled trial.

Purpose: To evaluate the analgesic efficacy of ultrasound-guided quadratus lumborum block (QLB) for laparoscopic renal surgery.

Methods: Sixty-eight patients who underwent unilateral laparoscopic renal surgery were enrolled. All patients were separated into two groups: group QLB (n = 34) and group C (n = 34).

Long-term nonferrous metal smelting affects metal accumulation/transfer in a soil-plant-woodlouse system.

Nonferrous metal smelting is one of the main sources of heavy metal pollution in the environment, yet its effect on heavy metal transfer in soil-plant-animal systems is not well investigated. In this work, soil, plant (Humulus scandens [HS], Digitaria sanguinalis [DS], and Setaria viridis [SV]), and woodlouse (Armadillidium vulgare) samples were collected in a long-term nonferrous metal smelting area from 26 sites over approximately 7 km and analyzed for Cd, Cu, Mn, Ni, Pb, and Zn concentrations. Cadmium and Pb were the most accumulated soil metals, with mean concentrations of 4.

Comparative Metagenomics Reveals Microbial Diversity and Biogeochemical Drivers in Deep-Sea Sediments of the Marcus-Wake and Magellan Seamounts.

Seamounts are distributed globally across the oceans and are generally considered oases of biomass abundance as well as hotspots of species richness. Diverse microbial communities are essential for biogeochemical cycling, yet their functional partitioning among seamounts with geographic features remains poorly investigated. Through metagenomic sequencing and genome-resolved analysis, we revealed that Proteobacteria (33.

Global Hotspots of Whale-Ship Collision Risk: A Multi-Species Framework Integrating Critical Habitat Zonation and Shipping Pressure for Conservation Prioritization.

The expansion of global maritime activities threatens marine ecosystems and biodiversity. Collisions between ships and marine megafauna profoundly impact vulnerable species such as whales, who serve as keystone predators. However, the specific regions most heavily affected by shipping traffic and the multi-species facing collision risk remain poorly understood.

Aerobic Exercise Delays Age-Related Sarcopenia in Mice via Alleviating Imbalance in Mitochondrial Quality Control.

Sarcopenia is a syndrome associated with aging, characterized by a progressive decline in skeletal muscle mass and function. Its onset compromises the health and longevity of older adults by increasing susceptibility to falls, fractures, and various comorbid conditions, thereby diminishing quality of life and capacity for independent living. Accumulating evidence indicates that moderate-intensity aerobic exercise is an effective strategy for promoting overall health in older adults and exerts a beneficial effect that mitigates age-related sarcopenia.

Microglia-neuronal communication mediated by P2X4R-BDNF-TrkB promotes synaptic plasticity and anterior cingulate cortex hyperactivity in muscle pain chronicity.

Background: Microglia-neuronal communication is crucial for the development and maintenance of pain. However, the exact mechanisms underlying this interaction and its role in anterior cingulate cortex (ACC) circuitry in pain regulation are under exploration.

Methods: We explored the role of P2X4R-brain-derived neurotrophic factor (BDNF)-TrkB signalling of ACC in regulating muscle pain (MP).

Cellulosic Functional Bioplastic with Tunable Strength and Toughness Through Heat-Treatment of Dynamic Covalent Networks.

The growing environmental crisis caused by petroleum-based polymers has intensified the development of sustainable alternatives, with many biomass-derived polymers demonstrating potential for degradability, renewability, and low carbon footprints, though these properties can vary depending on structure and processing. However, traditional bio-based systems often lack tunability in mechanical properties, making it challenging to achieve both high strength and ductility. Herein, we report a high-performance, recyclable bio-based film (CAF-L) constructed via Diels-Alder dynamic covalent chemistry between furfuryl-functionalized cellulose acetate and maleimide-modified lignin.

The effect of physical exercise on sleep quality in university students: chain mediation of health literacy and life satisfaction.

Objective: The present study was designed to explore the relationship between physical exercise and sleep quality. Specifically, it investigates the extent to which this relationship is mediated by a sequential process involving health literacy and life satisfaction.

Methods: Data on physical exercise, sleep quality, health literacy, and life satisfaction were collected from a sample of 12,646 college students (study participants) using a questionnaire.

Mitochondria-Targeting Abasic Site-Reactive Probe (mTAP) Enables the Manipulation of Mitochondrial DNA Levels.

Mitochondrial DNA (mtDNA) encodes essential genes for mitochondrial and cellular functions and acts as a cell signaling molecule in innate immune and inflammatory responses. Defects in mtDNA are implicated in a range of mitochondrial disorders and human diseases. Currently, no chemical strategy exists to prevent mtDNA loss under genotoxic stress.

Methods for Mitochondrial DNA Damage and Depletion in Immortalized Trabecular Meshwork Cells.

Mitochondrial DNA (mtDNA) damage in trabecular meshwork (TM) cells occurs in open-angle glaucoma (OAG). However, current in vitro models for OAG-like changes in TM cells do not explicitly incorporate mtDNA damage. This work validated two methods of mtDNA damage in immortalized TM cells and assessed OAG-associated expression changes.

The Effects of Different Types of Exercise on Pulmonary Inflammation and Fibrosis in Mice with Type 2 Diabetes Mellitus.

Diabetic lung disease, characterized by inflammation and fibrosis, is an emerging chronic complication of type 2 diabetes mellitus (T2DM). However, systematic studies on the effects of exercise interventions remain limited. This study aimed to investigate the impact of different exercise types (swimming, resistance training, and high-intensity interval training [HIIT]) on pulmonary inflammation and fibrosis in T2DM mice, and to explore underlying molecular mechanisms.

Exosome‑mediated crosstalk between the cardiovascular and musculoskeletal systems: Mechanisms and therapeutic potential (Review).

The cardiovascular and musculoskeletal systems are two core systems essential for maintaining human physiological functions and their dynamic interactions play a critical role in overall health. Exosomes, nanosized vesicles released by cells, contain bioactive substances including microRNA, long non‑coding RNA, lipids and proteins and participate in the pathophysiological regulation of multiple organ systems by mediating intercellular communication. Bone‑derived exosomes ameliorate cardiovascular diseases through the regulation of oxidative stress, inflammatory responses and apoptosis.

Marine Biodiversity Conservation Planning in the Indo-Pacific Convergence Zone Based on Ecological Spatial Analysis.

Marine biodiversity is of critical importance to global ecosystems. The Indo-Pacific Convergence Zone (IPCZ), a global marine biodiversity hotspot, faces escalating threats from human activities and climate change. This underscores the pressing need to develop effective conservation strategies for marine biodiversity in the IPCZ.

Carbon Dots-Enhanced Soy Protein Isolate/Polyvinyl Alcohol Composite Film for Active Preservation of Oxidation-Sensitive Foods.

Carbon dots perform a variety of functions when they are incorporated into active films synthesized from soy protein isolate/polyvinyl alcohol. The study examined the impact of varying concentrations of carbon dots on the structural, bioactive, and physicochemical properties of composite films. The addition of carbon dots improved the UV-blocking rate (up to 99.

β-Hydroxybutyrate dehydrogenase 1: A promising therapeutic target for diverse diseases.

β-Hydroxybutyrate dehydrogenase 1 (BDH1), a short-chain dehydrogenase located in the inner mitochondrial membrane, has been extensively studied for its critical role in ketone body metabolism. Recent research has expanded its known functions and revealed its involvement in the regulation of various physiological and pathological processes, including glucose metabolism, oxidative stress, inflammation, and apoptosis. As a result, BDH1's role in diverse pathological conditions, such as metabolic disorders, cancer, and cardiomyopathy, has garnered substantial research attention.

Trajectory of internet gaming disorder among Chinese adolescents: Course, predictors, and long-term mental health outcomes.

Background And Objectives: Internet gaming disorder (IGD) is a growing public health concern, especially among adolescents. However, most IGD studies are cross-sectional, leaving its developmental course poorly understood. This study examined IGD trajectories, their predictors, and their long-term mental health outcomes among Chinese adolescents.

Identification of autophagy-related biomarkers in prostate cancer prognosis.

Prostate cancer is the second leading cause of cancer-related deaths in males that has an unfavorable outcome. Autophagy-related genes (ARGs) contribute to the process of tumorigenesis and metastasis of prostate cancer. This study aimed to identify ARGs that could serve as reliable and non-invasive biomarkers for evaluating prostate cancer prognosis.

Dexamethasone and dexmedetomidine as adjuvants to local anesthetic mixture in erector spinae plane block combined with serratus anterior plane block for thoracoscopic surgery: a prospective, randomized controlled trial.

Background: We aimed to investigate the effect of dexamethasone and dexmedetomidine in erector spinae plane block combined with serratus anterior plane block for thoracoscopic postoperative analgesia.

Methods: Ninety-six patients undergoing thoracoscopic surgery were randomly divided into group R (0.375% ropivacaine + saline diluted to 40 mL), group RS (0.

Impact of tourniquet application on postoperative delirium in elderly patients undergoing total knee arthroplasty: a randomized clinical trial.

Background: Tourniquet is widely used in total knee arthroplasty (TKA), but its impact on postoperative delirium (POD) remains unclear. The purposes of this study were to investigate the impact of tourniquet application on POD in elderly patients TKA and to explore the possible mechanisms associated with POD.

Materials And Methods: In this prospective, single-center randomized clinical trial study, 313 patients scheduled for single TKA under general anesthesia were randomly assigned to groups with or without a limb tourniquet.