AoChk1 Is Required for Sporulation, Trap Formation, and Metabolic Process in .

Chk1, a highly conserved serine/threonine protein kinase, functions as a critical regulator of fungal cell cycle progression, mitotic fidelity, and DNA damage response. In this study, we characterized an orthologous Chk1 (AoChk1) in a ubiquitous nematode-trapping fungus, through targeted gene knockout coupled with integrated phenotypic, metabolomic, and transcriptomic analyses. This study aims to elucidate the function and potential regulatory networks of AoChk1 in .

Self-healing hydrogels loaded with selenium nanoparticles/chitosan/cellulose nanofibers as carriers of mesenchymal stem cells for diabetic wound healing.

As one of the most serious chronic complications of diabetes mellitus, diabetic foot ulcers (DFUs) have a complex pathogenesis involving multiple factors such as persistent hyperglycemia-induced microangiopathy, chronic inflammatory state and oxidative stress injury. Regenerative medicine strategies of stem cells have brought new hope for DFUs treatment, among which adipose-derived mesenchymal stem cells (ADSCs) have attracted much attention due to their easy accessibility, multidirectional differentiation potential and paracrine function. However, excessive accumulation of reactive oxygen species (ROS) due to the high-glucose microenvironment can trigger mitochondrial dysfunction and premature senescence of ADSCs, which greatly reduce their therapeutic efficacy.

Harnessing Text Insights with Visual Alignment for Medical Image Segmentation.

Pre-trained vision-language models (VLMs) and language models (LMs) have recently garnered significant attention due to their remarkable ability to represent textual concepts, opening up new avenues in vision tasks. In medical image segmentation, efforts are being made to integrate text and image data using VLMs and LMs. However, current text-enhanced approaches face several challenges.

Construction of a multimodal esophageal cancer prognostic model with screening of key genes and investigation of their biological functions.

Purpose: Despite decreased esophageal carcinoma incidence, its prognosis remains challenging. We aimed to develop a multimodal prognostic model to optimize treatment strategies and elucidate biological mechanisms of key regulatory genes.

Methods: Pivotal prognostic genes were screened using integrated bioinformatics and immunohistochemistry.

Lang-chuang-ding restores bone homeostasis in systemic lupus erythematosus associated osteoporosis by targeting NF-κB signaling: a network pharmacology and experimental study.

Systemic lupus erythematosus (SLE) is frequently associated with secondary osteoporosis (OP), substantially compromising patients' quality of life. Although Lang-chuang-ding (LCD), a traditional Chinese medicine formulation, has demonstrated efficacy in suppressing SLE progression, its therapeutic potential for SLE-associated OP remains uninvestigated. This study investigated the therapeutic effects and underlying pharmacological mechanisms of LCD on SLE-associated OP through experimental validation using MRL mouse model in conjunction with network pharmacology analysis.

Digital Interventions for Improving Body Dissatisfaction in Children and Emerging Adults: Systematic Review and Meta-Analysis.

Background: Body dissatisfaction is a condition where individuals are dissatisfied with their physical appearance. It has become a global issue, especially among children and emerging adults. A growing number of digital interventions have been developed to address body dissatisfaction in children and emerging adults; however, controversies remain regarding their efficacy, underscoring the need for a comprehensive synthesis of current evidence.

Morphological and phylogenetic analyses reveal two novel species of (Pleosporales, Pleosporaceae) from southern China.

Members of are of widespread clinical and agricultural importance, but species identification remains challenging due to morphological similarities and environmental variation. In this study, nine strains were isolated from diseased leaves of and in Guangxi and Guizhou provinces, China. Morphological characteristics and phylogenetic analyses based on ITS, , and sequences revealed two novel species of s.

Subchronic Chlorpyrifos Exposure Induces Thyroid Follicular Cell Pyroptosis to Exacerbate Thyroid Toxicity by Modulating Nrf2/Keap1/NF-κB Pathway in Male Mice.

Purpose: Chlorpyrifos (CPF), a widely used organophosphate pesticide in agriculture, particularly in China, has raised significant environmental and health concerns due to its persistence and bioaccumulation. While CPF-induced toxicity in multiple organ systems has been documented, its long-term impact on thyroid homeostasis and the underlying mechanisms remain poorly understood. This study aimed to investigate the subchronic effects of CPF on thyroid function and elucidate the underlying mechanisms of CPF-induced thyroid toxicity.

Sustained Synthesis of Ultra-Long Gold Wires via an Open Microfluidic System.

Ultra-long metal nano/microwires have broad applications in fields such as electronics, sensing, and catalysis. With the advantage of laminar flow, the microfluidic approach enables the efficient synthesis of metal micro- and even nanowires. However, the synthesized metal wires typically broaden over time due to diffusion as their length increases within microchannels, making it challenging to achieve long metal wires without widening.

An Inclined Push-Pull Probe for In Situ Cell Staining and Calcium Channel Activation.

Microfluidics is now extensively used for cellular analysis due to its merits of sensitivity, accuracy, and miniaturization. The push-pull probe is built on microfluidic principles but innovatively uses hydrodynamic forces instead of closed microchannels for liquid confinement. Although various microfluidic probes have been developed for cell manipulation and analysis, few have addressed the integration and optical challenges posed by vertically oriented probes, which are difficult to accommodate in compact experimental setups and often interfere with optical imaging pathways.

Bisphenol AF Induced Neurodevelopmental Toxicity of Human Neural Progenitor Cells via Nrf2/HO-1 Pathway.

Bisphenol AF (BPAF) is widely utilized as an analog of bisphenol A (BPA) in the plastics industry. However, there is limited evidence on its neurodevelopmental toxicity. Existing studies suggest that BPAF has greater accumulation in vivo than other bisphenol analogs, and could pass through the placental barrier and the blood-brain barrier.

Degradation of Organophosphorus Pollutants into Inorganic Phosphates by Co(II)/NaBO System and Its Phosphorus Recycling.

With the increase of demand of phosphorus resources and its natural shortage, circulating utilization of organophosphorus contaminants into phosphatic fertilizer could provide a sustainable way to achieve the phosphorus cycle. Herein, a homogeneous catalytic system of Co(II)/sodium peroxyborate (NaBO·4HO, SPB) was developed for the degradation of 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP) and glyphosate (PMG) into inorganic phosphate (-PO). The Co(II)/SPB system maintained excellent catalytic performance for HEDP degradation under a broad initial pH range (2.

Changes in pulmonary function of CT-guided microwave ablation for patients with pulmonary ground-glass nodules after lobectomy: a retrospective, observation study.

Purpose: This retrospective study aimed to investigate changes in pulmonary function among patients undergoing computed tomography (CT)-guided microwave ablation (MWA) for the treatment of pulmonary ground-glass nodules following lobectomy.

Materials And Methods: Fifty-two patients with pulmonary ground-glass nodules (GGN) who underwent lobectomy and were subsequently diagnosed with non-small cell lung cancer (NSCLC) were included in the study. Pulmonary function tests (PFTs) were conducted at baseline and again 1 month after microwave ablation (MWA).

[Molecular mechanisms of microbial mercury resistance and their prospective applications in remediation of mercury-contaminated soils].

Mercury (Hg)-contaminated soil poses a significant threat to the environment and human health. Hg-resistant microorganisms have the ability to survive under the stress of inorganic and organic Hg and effectively reduce Hg levels and toxicity. Compared to physical and chemical remediation methods, microbial remediation technologies have garnered increasing attention in recent years due to their lower cost, remarkable efficacy, and minimal environmental impact.

Clinical Characterization of Long COVID Among Chinese Healthcare Workers: A Multicenter, Cross-sectional Study.

Objectives: Since the outbreak of COVID-19, the pandemic has profoundly affected global health and socioeconomic systems. Many recovered patients continue to experience "long COVID" symptoms, affecting their daily life and work performance.

Methods: We utilized questionnaires to investigate long COVID symptoms among healthcare workers and their impact on daily life and work.

Adversarial testing of global neuronal workspace and integrated information theories of consciousness.

Different theories explain how subjective experience arises from brain activity. These theories have independently accrued evidence, but have not been directly compared. Here we present an open science adversarial collaboration directly juxtaposing integrated information theory (IIT) and global neuronal workspace theory (GNWT) via a theory-neutral consortium.

A biomimetic manganese-phycocyanin nanodrug-carrying system and its sonodynamic-immunological anti-tumor therapy.

Cutaneous melanoma is characterized by malignant proliferation, high aggressiveness, high metastasis, rapid recurrence, and low survival rate; therefore, research on its treatment is vital. In this study, a novel nano system combining sonodynamics and immunotherapy for cutaneous melanoma treatment was designed and developed. Based on the use of phycocyanin for the biomineralization of manganese ions, smart and multifunctional manganese phycocyanin nanoparticles encapsulating the immune adjuvant levamisole (LMS) with melanoma B16-OVA cell membranes wrapped around its outer layer (Mn-PCNP-LMS@CM) were designed and prepared.