Clinical features and genetic analysis of A20 haploinsufficiency.

Objective: To described clinical and genetic characteristics of 4 patients presenting A20 haploinsufficiency (HA20) treated at Children's hospital affiliated to Zhengzhou university from 2015 to 2024.

Methods: A retrospective analysis was conducted on the clinical data, genetic testing results, and treatment outcomes of four children with HA20 treated at the Children's hospital affiliated to Zhengzhou university from 2015 to 2024.

Results: All four patients developed symptoms before the age of 1 year, presenting with recurrent fever and abdominal pain with diarrhea.

Lactate regulates cell differentiation of erythroid progenitor cells via histone lactylation modification.

Lactate plays important regulatory roles in a variety of biological events by regulating metabolic homeostasis and histone lactylation. However, the role of lactate and histone lactylation in human erythropoiesis remains unclear. Here, we explored the role of lactate in erythropoiesis by adding the glycolysis inhibitor 2-deoxy--glucose (2-DG) or exogenous lactate Na-La to decrease or increase intracellular lactate levels.

A Novel Supramolecular Salt of Hypoxanthine with Maleic Acid as a Potential Weight-Loss Drug.

Improving the stability of drugs in the solid state, as well as improving their solubility and poor bioavailability, is highly physiologically relevant. In this study, we focused on enhancing the solubility of hypoxanthine (HYP) through salt formation resulting from the preparation of hypoxanthine-maleic acid salt (HYP-MAL). Single crystals were obtained through solvent evaporation methods, and DSC, TGA, PXRD, FT-IR, and H NMR spectra were used to characterize the samples.

IDH1 regulates human erythropoiesis by eliciting chromatin state reprogramming.

Isocitrate dehydrogenase 1 (IDH1) is the key enzyme that can modulate cellular metabolism, epigenetic modification, and redox homeostasis. Gain-of-function mutations and decreased expression of IDH1 have been demonstrated to be associated with pathogenesis of various myeloid malignancies characterized by ineffective erythropoiesis, such as acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). However, the function and mechanism of IDH1 in human erythropoiesis still remains unclear.

Solvates and Polymorphs of Axitinib: Characterization and Phase Transformation.

Axitinib (AXTN) is an oral tyrosine kinase inhibitor for the treatment of early to advanced renal cell carcinoma. In this work, solvates of AXTN were prepared in five solvents and subjected to desolvation treatment. The crystal form A of AXTN can form solvates in acetonitrile, DMF, acetic acid, acetic acid + water, and methanol.

Effect of Additives on the Morphology of γ-Aminobutyric Acid Crystals.

The effect of surfactant, polymer, and tailor-made additives on the crystallization of γ-aminobutyric acid (GABA) was studied in this work. Cooling crystallization of GABA in water yielded plate-like crystals. In the presence of sodium stearate, polyhedral block-like crystals of GABA were obtained.

[Risk factors for delayed bleeding after intestinal polypectomy in children].

Objectives: To investigate the clinical characteristics and risk factors of delayed bleeding after intestinal polypectomy in children, and to provide a theoretical basis for clinical surgical intervention of intestinal polyps.

Methods: A retrospective analysis was conducted on the clinical data of 2 456 children with intestinal polyps who underwent endoscopic high-frequency electrocoagulation loop resection in the Endoscopy Center of Children's Hospital Affiliated to Zhengzhou University from January 2014 to December 2021. According to the presence or absence of delayed bleeding after surgery, they were divided into bleeding group with 79 children and non-bleeding group with 2 377 children.

Determination and Correlation of Solubility of -Acetylglucosamine in Four Aqueous Binary Solvents from 283.15 to 323.15 K.

-Acetylglucosamine (NAG) is a significant novel functional monosaccharide with a wide range of potential applications, such as in the medical and cosmetic fields. A gravimetric technique was used to assess the solubility of NAG in water, methanol, ,-dimethylformamide, water + methanol, water + -propanol, water + ,-dimethylformamide, and water + acetonitrile at temperatures ranging from 283.15 to 323.

The impact of erythroblast enucleation efficiency on the severity of anemia in patients with myelodysplastic syndrome.

Anemia is the most common manifestation in myelodysplastic syndrome (MDS) patients, but the cause of ineffective hematopoiesis is not fully understood. Enucleation is an important event in the maturation process of erythroblasts. According to a series of morphological phenotypes of the pathological development of MDS erythroblasts, we speculate that there may be enucleation disorders.

Refractive Index of 48 Neat Deep Eutectic Solvents and of Selected Mixtures: Effect of Temperature, Hydrogen-Bonding Donors, Hydrogen-Bonding Acceptors, Mole Ratio, and Water.

The refractive index (RI) is an important physiochemical property of deep eutectic solvents (DESs) for application in the optical identification of specific substances or measuring the concentration of solutes in solutions. However, the available data on the RI of DESs is limited. Here, a systematic investigation on the RI of 48 typical DESs and 30 mixtures with water was conducted under atmospheric pressure.

Polymer-mediated and ultrasound-assisted crystallization of ropivacaine: Crystal growth and morphology modulation.

The objective of this research was to modify the crystal shape and size of poorly water-soluble drug ropivacaine, and to reveal the effects of polymeric additive and ultrasound on crystal nucleation and growth. Ropivacaine often grow as needle-like crystals extended along the a-axis and the shape was hardly controllable by altering solvent types and operating conditions for the crystallization process. We found that ropivacaine crystallized as block-like crystals when polyvinylpyrrolidone (PVP) was used.

Hot Water Extraction of Antioxidants from Tea Leaves-Optimization of Brewing Conditions for Preparing Antioxidant-Rich Tea Drinks.

There are billions of tea drinkers around the world. However, the optimized tea-brewing temperature and time conditions for achieving a higher concentration of antioxidants in tea drinks have not been thoroughly studied. Finding out the optimized brewing conditions can benefit tea drinkers significantly.

Stage-specific dual function: EZH2 regulates human erythropoiesis by eliciting histone and non-histone methylation.

Enhancer of zeste homolog 2 (EZH2) is the lysine methyltransferase of polycomb repressive complex 2 (PRC2) that catalyzes H3K27 tri-methylation. Aberrant expression and loss-of-function mutations of EZH2 have been demonstrated to be tightly associated with the pathogenesis of various myeloid malignancies characterized by ineffective erythropoiesis, such as myelodysplastic syndrome (MDS). However, the function and mechanism of EZH2 in human erythropoiesis still remains largely unknown.

DNA methylation status of is essential for human erythropoiesis.

To investigate DNA methylation patterns in early and terminal stages of erythropoiesis, and to explore the function of differentially methylated genes in erythropoiesis and erythroid disorders. Differential analysis of DNA methylation and gene expression during erythropoiesis, as well as weighted gene coexpression network analysis of acute myeloid leukemia was performed. We identified four candidate genes that possessed differential methylation in the promoter regions.

Solid-Liquid Equilibrium Behavior and Solvent Effect of Gliclazide in Mono- and Binary Solvents.

The solubility data of gliclazide in 10 mono-solvents (1,2-dichloroethane, 1,4-dioxane, 2-methoxyethanol, -propyl acetate, isopropyl acetate, -butyl acetate, pentyl acetate, dimethyl sulfoxide (DMSO), ,-dimethylacetamide (DMA), and 2-butanone) and one kind of binary solvent (DMA + water) were measured between 278.15 and 323.15 K under atmospheric pressure by the gravimetric method.

An Injectable Hydrogel to Modulate T Cells for Cancer Immunotherapy.

T cell exhaustion caused by mitochondrial dysfunction is the major obstacle of T cells-based cancer immunotherapy. Besides exhausted T cells, the insufficient major histocompatibility complex class I (MHC I) on tumor cells leads to inefficient T cell recognition of tumor cells, compromising therapeutic efficacy. Therapeutic platform to regulate T cell exhaustion and MHC I expression for boosting T cells-based cancer immunotherapy has not been realized up to date.

A carrier-free photodynamic nanodrug to enable regulation of dendritic cells for boosting cancer immunotherapy.

Immune response is initiated by dendritic cells (DCs), where the cross-presentation of antigens by DCs determines the activating of cytotoxic T cells. However, the efficacy of DCs-initiated immune response is governed by multiple (cascade) steps of immunogenic cell death (ICD), recruitment of DCs, and cross-presentation of DCs. It is urgent but challenging to achieve a platform for simultaneously regulating these multiple steps, amplifying the immune response against tumors.

Determination and Correlation of Solubility of Metformin Hydrochloride in Aqueous Binary Solvents from 283.15 to 323.15 K.

Metformin hydrochloride (MET·HCl) is one of the most widely used oral hypoglycemic drugs in the world. In addition to hypoglycemic effects, MET·HCl also has anti-inflammatory, anti-tumor, anti-aging, and other effects, showing good efficacy and safety of single and combined treatment. The solubility of MET·HCl in water, water + ,-dimethylformamide, water + acetonitrile, and water + -propanol was measured by the gravimetric method under atmospheric pressure at temperatures ranging from 283.

Laser ablative TiO and tremella-like CuInS nanocomposites for robust and ultrasensitive photoelectrochemical sensing of let-7a.

A photoelectrochemical (PEC) biosensor based on a multiple signal amplification strategy was established for highly sensitive detection of microRNA (miRNA). TiO was prepared on the surface of titanium sheet by laser etching to improve its stability and photoelectrical properties, and CuInS-sensitized TiO was used to form a superior photoelectrical layer, which realized the initial signal amplification. The electron donor dopamine (DA) was modified to H2 as a signal regulator, which effectively increased the photocurrent signal.

Vesicular formation regulated by ERK/MAPK pathway mediates human erythroblast enucleation.

Enucleation is a key event in mammalian erythropoiesis responsible for the generation of enucleated reticulocytes. Although progress is being made in developing mechanistic understanding of enucleation, our understanding of mechanisms for enucleation is still incomplete. The MAPK pathway plays diverse roles in biological processes, but its role in erythropoiesis has yet to be fully defined.

Subcritical Water Extraction of Natural Products.

Subcritical water refers to high-temperature and high-pressure water. A unique and useful characteristic of subcritical water is that its polarity can be dramatically decreased with increasing temperature. Therefore, subcritical water can behave similar to methanol or ethanol.

Electrochemiluminescence ultrasensitive immunoassay for carbohydrate antigen 125 based on AgInS/ZnS nanocrystals.

We developed a near-infrared (NIR) electrochemiluminescence (ECL) immunosensor for sensitively and selectively determining carbohydrate antigen 125 (CA125) with toxic-element-free and environmental-friendly AgInS/ZnS nanocrystals (NCs) as tags. The core/shell-structured AgInS/ZnS NCs not only can be conveniently prepared via an aqueous synthetic procedure, but also has high photoluminescence quantum yield (PLQY) of up to 61.7%, highly monodispersed, water-soluble, and desired biological compatibility.