Novel pleuromutilin derivatives with substituted 6-methylpyrimidine: Design, synthesis and antibacterial evaluation.

A series of novel pleuromutilin derivatives with substituted 6-methylpyrimidine moieties was designed, synthesized, and evaluated for their antibacterial activities. Most of the tested compounds exhibited potent antibacterial activities against Staphylococcus aureus ATCC 25923 (S. aureus-25923), methicillin-resistant Staphylococcus epidermidis ATCC 51625 (MRSE-51625), methicillin-resistant Staphylococcus aureus BNCC 337371 (MRSA-337371), Streptococcus dysgalactiae (S.

A Single-Transaminase-Catalyzed Biocatalytic Cascade for Efficient Asymmetric Synthesis of l-Phosphinothricin.

A single-transaminase-catalyzed biocatalytic cascade was developed by employing the desired biocatalyst, ATA-117-Rd11, that showed high activity toward 2-oxo-4-[(hydroxy)(methyl)phosphinoyl] butyric acid (PPO) and α-ketoglutarate, and low activity against pyruvate. The cascade successfully promotes a highly asymmetric amination reaction for the synthesis of l-phosphinothricin (l-PPT) with high conversion (>95 %) and>99 % ee. In a scale-up experiment, using 10 kg pre-frozen E.

Genotype‒Phenotype Correlation of TRPV3-Related Olmsted Syndrome.

We have previously shown that gain-of-function variations in transient receptor potential vanilloid-3 (TRPV3) underlay Olmsted syndrome, a rare hyperkeratotic skin channelopathy. In this study, we attempt to establish a genotype‒phenotype correlation in Olmsted syndrome, which has been unclear owing to the rarity and heterogeneity of the condition. We identified five previously unreported TRPV3 variations (R416Q, R416W, L655P, W692S, and L694P) and three recurrent variations (G568D, G568V, and L673F) in nine unrelated patients.

All-Optical Manipulation of Magnetization in Ferromagnetic Thin Films Enhanced by Plasmonic Resonances.

In this paper, we report all-optical manipulation of magnetization in ferromagnetic Co/Pt thin films enhanced by plasmonic resonances. By annealing a thin Au layer, we fabricate large-area Au nanoislands on top of the Co/Pt magnetic thin films, which show plasmonic resonances around the wavelength of 606 nm. Using a customized magneto-optical Kerr effect setup, we experimentally observe an 18.

Dual Energy Transfer-Based DNA/Graphene Oxide Nanocomplex Probe for Highly Robust and Accurate Monitoring of Apoptosis-Related microRNAs.

Fluorescent labeled single-stranded DNA (ssDNA) molecules physisorbed on graphene oxide (GO) have been extensively explored as a useful sensing platform. However, this approach faces challenges when applied to complex biological samples due to heavy nonspecific desorption of nontarget molecules from GO. To overcome this problem, we introduced a capture DNA (cDNA) fragment with a poly adenine (poly-A) extension into the physisorption system that greatly reduces nonspecific desorption and false positive signal due to strong binding between poly-A and GO.

Improved Brassica oleracea JZS assembly reveals significant changing of LTR-RT dynamics in different morphotypes.

Brassica oleracea is an important vegetable crop that has provided ancestor genomes of the two most important Brassica oil crops, Brassica napus and Brassica carinata. The current B. oleracea reference genome (JZS, also named 02-12) displays problems of large mis-assemblies, low sequence continuity, and low assembly integrity, thus limiting genomic analysis.

Characterization of the Noise Induced by Stimulated Brillouin Scattering in Distributed Sensing.

The excess noise due to stimulated Brillouin scattering (SBS) in gain and loss-based Brillouin optical time-domain analyzers (BOTDA) has been investigated theoretically and experimentally for the first time to the best of our knowledge. This investigation provides a full insight to the SBS-induced noise distribution, which mainly comes from phase-to-intensity conversion noise and the beating noise between the probe wave and spontaneous Brillouin scattering. A complete theoretical model, which is in good agreement with the experimental results, is presented to describe the noise.

Early wound complications after orthopaedic surgery for haemophilia: What can we do more.

Background: The characteristics of the haemophilia may endanger the patient with haemophilia (PWH) to higher ratio of wound complication, even lead to the refractory wound problem. The early wound complication in PWH has not been well studied in literature.

Methods: Between the period from 2002 to 2018, 250 patients underwent 323 elective orthopaedic surgical procedures for the treatment of haemophilic musculoskeletal disorders.

Cardiac manifestations of COVID-19 in Shenzhen, China.

Purpose: The coronavirus disease 2019 (COVID-19) outbreak has become a global public health concern; however, relatively few detailed reports of related cardiac injury are available. The aims of this study were to compare the clinical and echocardiographic characteristics of inpatients in the intensive-care unit (ICU) and non-ICU patients.

Methods: We recruited 416 patients diagnosed with COVID-19 and divided them into two groups: ICU (n = 35) and non-ICU (n = 381).

Removal of contaminants by activating peroxymonosulfate (PMS) using zero valent iron (ZVI)-based bimetallic particles (ZVI/Cu, ZVI/Co, ZVI/Ni, and ZVI/Ag).

In this study, four different ZVI/M-PMS systems (, ZVI/Cu, ZVI/Co, ZVI/Ni and ZVI/Ag) were fabricated to investigate the removal of contaminants (Rhodamine B (RB), 2,4-dichlorophenol (2,4-DCP), bisphenol A (BPA), bisphenol F (BPF), levofloxacin (LFX), and chloramphenicol (CAP)). The results indicated that ZVI/Cu and ZVI/Ag exhibited a superior performance to activate PMS compared with the ZVI. The mechanism of the investigation showed that a relatively positive correlation between the release of iron ions (Fe) and contaminant removal efficiency was found in different ZVI/M-PMS systems.

Characterization of resin extracted from guayule (): A dataset including GC-MS and FT-ICR MS.

Guayule (), a shrub native to the arid region of the U.S. southwest and Mexico belonging to the Asteraceae family, is a source of high quality, hypoallergenic natural rubber with applications in pharmaceutical, tire, and food industries.

Enhancement of bisphenol A degradation by accelerating the Fe(III)/Fe(II) cycle in graphene oxide modified Fe(III)/peroxymonosulfate system under visible light irradiation.

The photocatalytic behavior of the graphene oxide (GO) modified Fe(III)/peroxymonosulfate (Fe/PMS) system for bisphenol A (BPA) degradation was investigated. With the addition of GO, a dramatic enhancement of BPA degradation was obtained at pH 3.0 under visible light irradiation.

Overexpression of microRNA-203 Suppresses Proliferation, Invasion, and Migration while Accelerating Apoptosis of CSCC Cell Line SCL-1.

Cutaneous squamous cell carcinoma (CSCC) is a malignant proliferation of cutaneous epithelium that has been observed to have an alarming rise in incidence. Numerous studies have demonstrated microRNAs (miRNAs or miRs) as important biomarkers in the diagnosis, prognosis, and treatment of CSCC. This study aims to investigate the effects of miR-203 on the behaviors of CSCC cells and possible mechanisms associated with protein regulator of cytokinesis-1 (PRC1) and Wnt/β-catenin signaling pathway.

Diabetes induces hepatocyte pyroptosis by promoting oxidative stress-mediated NLRP3 inflammasome activation during liver ischaemia and reperfusion injury.

Background: Although diabetes mellitus has been reported to aggravate liver ischaemia and reperfusion (IR) injury, the basic mechanism remains largely unknown. The object of the present study was to determine the role of oxidative stress and hepatocellular pyroptosis in liver IR injury in diabetic mice.

Methods: Db/db and C57BL/6 mice at 8 weeks of age were subjected to liver IR injury.

Probing of coupling effect induced plasmonic charge accumulation for water oxidation.

A key issue for redox reactions in plasmon-induced photocatalysis, particularly for water oxidation, is the concentration of surface-accumulating charges (electrons or holes) at a reaction site for artificial photosynthesis. However, where plasmonic charge accumulated at a catalyst's surface, and how to improve local charge density at active sites, remains unknown because it is difficult to identify the exact spatial location and local density of the plasmon-induced charge, particularly with regard to holes. Herein, we show that at the single particle level, plasmon-coupling-induced holes can be greatly accumulated at the plasmonic Au nanoparticle dimer/TiO interface in the nanogap region, as directly evidenced by the locally enhanced surface photovoltage.

Analysis of lncRNAs-miRNAs-mRNAs networks in periodontal ligament stem cells under mechanical force.

Objectives: Our study aims to analyze the expression profiles of long non-coding RNAs (lncRNAs) and investigate the potential regulatory networks among lncRNAs, microRNAs (miRNAs), and mRNAs in periodontal ligament stem cells (PDLSCs) under mechanical force (MF).

Materials And Methods: PDLSCs were isolated from human periodontal ligament tissues and identified by flow cytometry analysis. Multidirectional differentiation potential of PDLSCs was obtained by osteogenic and adipogenic induction.

Comparison of responses of melanocyte lineages from p75(+) and p75(-) human scalp-derived neural crest stem cells under phototherapy.

Phototherapy is an effective therapeutic option in the treatment of vitiligo; however, responses varied among the different types. The underlying mechanism has scarcely been investigated. To investigate and compare the effects of phototherapy on the mutation of melanocyte lineage differentiated from human scalp-derived neural crest stem cells (HS-NCSCs) with p75 neurotrophin receptor expression positive and p75 neurotrophin receptor expression negative group in vitro, the HS-NCSCs were isolated from fetal scalp tissue, which is identified by immunofluorescent staining.

[Therapeutic effect analysis of skeletal class Ⅲ malocclusion treatment by transmission straight wire technique].

Objective: This study aimed to investigate the therapeutic effects and advantages of skeletal class Ⅲ malocclusion treatment by transmission straight wire technique.

Methods: Ninety-seven patients who received treatment for skeletal class Ⅲ malocclusion at the Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology from January 2012 to January 2017 were selected for this retrospective study. All these patients refused surgery.

Machine Learning-Based Signal Quality Evaluation of Single-Period Radial Artery Pulse Waves: Model Development and Validation.

Background: The radial artery pulse wave is a widely used physiological signal for disease diagnosis and personal health monitoring because it provides insight into the overall health of the heart and blood vessels. Periodic radial artery pulse signals are subsequently decomposed into single pulse wave periods (segments) for physiological parameter evaluations. However, abnormal periods frequently arise due to external interference, the inherent imperfections of current segmentation methods, and the quality of the pulse wave signals.

A two-stage bilateral ischemia-reperfusion injury-induced AKI to CKD transition model in mice.

Acute kidney injury (AKI) significantly increases the risk of development of chronic kidney disease (CKD). Recently, our laboratory generated a mouse model with the typical phenotypes of AKI to CKD transition in the unilateral kidney. However, AKI, CKD, and even the transition from AKI to CKD usually occur bilaterally rather than unilaterally in patients.

Correction to: DNA typing from skeletal remains: a comparison between capillary electrophoresis and massively parallel sequencing platforms.

The name of an author was misspelled. The correct spelling is "Linlin Gao" instead of Linin Gao.

[Monitoring and evaluation of eco-environmental quality of lake basin regions in Central Yunnan Province, China].

The basin region of nine highland lakes plays an important role in the economic development of Yunnan Province, in which, the basin region of the five lakes (Dianchi, Fuxianhu, Xingyunhu, Qiluhu and Yangzonghai Lake, hereinafter was called Five-lake Basin) locating in the central region of Yunnan Province is the most active economic and social development area of industry, agriculture and tourism, closely related to the eco-environmental quality of the whole basin. In this study, the eco-environmental quality of the Five-lake Basin from 1988 to 2018 was monitored and evaluated based on remote sensing ecological index (RSEI). The results showed that although the eco-environmental quality in this area had a trend of turning better from 1988 to 2018, with the mean value of RSEI increasing from 0.

Strategies and prospects of effective neural circuits reconstruction after spinal cord injury.

Due to the disconnection of surviving neural elements after spinal cord injury (SCI), such patients had to suffer irreversible loss of motor or sensory function, and thereafter enormous economic and emotional burdens were brought to society and family. Despite many strategies being dealing with SCI, there is still no effective regenerative therapy. To date, significant progress has been made in studies of SCI repair strategies, including gene regulation of neural regeneration, cell or cell-derived exosomes and growth factors transplantation, repair of biomaterials, and neural signal stimulation.

A Chinese case of Nakajo-Nishimura syndrome with novel compound heterozygous mutations of the PSMB8 gene.

Background: Nakajo-Nishimura syndrome (NNS) is an autosomal recessive heredity disorder, one of a spectrum of autoinflammatory diseases named proteasome-associated autoinflammatory syndrome (PRAAS) caused by mutations of PSMB8 gene. NNS is characterized by pernio-like skin rashes, intermittent fever, and long clubbed fingers and toes with joint contractures, partially with progressive lipomuscular atrophy, emaciation, hepatosplenomegaly and basal ganglion calcification.

Case Presentation: We presented a sporadic case of NNS with compound heterozygous mutations in the PSMB8 gene.

DNA typing from skeletal remains: a comparison between capillary electrophoresis and massively parallel sequencing platforms.

Skeletal remains encountered frequently in forensic applications are a challenging specimen, since their DNA is usually degraded due to harsh conditions, limiting the utilization of skeletal DNA. Forensic scientists have tried various methods to extract DNA from skeletal remains of low quantity and poor quality or improve detecting technology for more information from compromised DNA. Compared with traditional capillary electrophoresis (CE), massively parallel sequencing (MPS) is more sensitive to shorter fragments, able to detect allele sequences for variations from core motif or flanking regions, and able to detect more markers with a higher discrimination power.