Protective Role of Bre1 in Mitochondrial Function and Energy Metabolism in Drosophila Models of Parkinson's disease.

Background: The second most common cause of autosomal recessive early-onset Parkinson's disease (PD) can be attributed to mutations in the PINK1 gene, malfunction of the mitochondria is the key pathological mechanism. Bre1 encodes an E3 ubiquitin ligase, with the discovery of Bre1's role in repairing mitochondrial damage, further investigation into its implications for PD is warranted.

Methods: We used the PINK1B9 drosophila melanogaster as the PD model.

PDZK1 Protects Against RPE Senescence by Targeting the 14-3-3ε-mTOR Axis to Attenuate Early Diabetic Retinopathy.

Diabetic retinopathy (DR) is the leading cause of blindness among working-age adults, yet its pathogenesis remains incompletely understood. The retinal pigment epithelium (RPE) plays a vital role in maintaining retinal homeostasis. In this study, the expression of senescence marker protein p16 is observed to be upregulated in the RPE of early DR mouse models.

Antiferromagnetic Dimanganese Catalase Mimics: Targeting Oxidative Stress to Mitigate Corneal Neovascularization Post-alkali Injury.

Corneal alkali-burn injury is a common ophthalmic emergency that elevates oxidative stress in the ocular microenvironment, leading to cellular apoptosis and corneal neovascularization. To address this challenge, we report a binuclear manganese complex () featuring a catalase-mimetic antiferromagnetic Mn-O-Mn core, which efficiently scavenges hydrogen peroxide, a key contributor to oxidative damage. This structure enables effective hydrogen peroxide scavenging while concurrently suppressing the Fenton reaction, a major driver of oxidative injury.

Analysis of the current situation and influencing factors of home treatment for children with hemophilia in China.

Objective: To investigate the current status of home treatment for children with hemophilia in China and identify key influencing factors.

Methods: Based on a comprehensive literature review and expert consultation, the Questionnaire on Home Treatment for Children with Hemophilia was developed. The study targeted fathers or mothers of children with hemophilia who were registered on the official website of "Home for Hemophilia Patients.

Dual U-Net with multi-task attention for automated eyelid curvature quantification.

Objective: Eyelid curvature analysis serves as a key morphological indicator in the diagnosis of ophthalmic diseases and postoperative evaluation. This study aims to develop an automated and reproducible image processing method to accurately extract eyelid margin curves from anterior segment images and perform quantitative curvature analysis.

Methods: A dual-branch U-Net architecture is proposed, utilizing a shared encoder and task-specific decoders to simultaneously segment the palpebral fissure and corneal regions.

Research Progress in Artificial Intelligence for Central Serous Chorioretinopathy: A Systematic Review.

Introduction: This review synthesizes advancements in artificial intelligence (AI) applications for central serous chorioretinopathy (CSCR), analyzing challenges and outlining future research directions to guide personalized diagnostic and therapeutic strategies.

Methods: A systematic literature search was conducted in the Web of Science database using a comprehensive AI-related keyword set (e.g.

Artificial intelligence in pathologic myopia: a review of clinical research studies.

Myopia is a significant global health challenge, with the incidence of pathologic myopia (PM) on the rise. PM-related fundus diseases have become a leading cause of irreversible blindness. Early detection and treatment are crucial for the prevention and control of myopia.

Comparative genomic analysis of 255 isolates from China: unveiling strain diversity and genotype-phenotype associations of acid resistance.

the only species of lactic acid bacteria capable of fully completing malolactic fermentation under challenging wine conditions, continues to intrigue researchers owing to its remarkable adaptability, particularly in combating acid stress. However, the mechanism underlying its superior adaptation to wine stresses still remains elusive due to the lack of viable genetic manipulation tools for this species. In this study, we conducted genomic sequencing and acid resistance phenotype analysis of 255 isolates derived from diverse wine regions across China, aiming to elucidate their strain diversity and genotype-phenotype associations of acid resistance through comparative genomics.

Accurate cross-species 5mC detection for Oxford Nanopore sequencing in plants with DeepPlant.

Nanopore sequencing enables comprehensive detection of 5-methylcytosine (5mC), particularly in repeat regions. However, CHH methylation detection in plants is limited by the scarcity of high-methylation positive samples, reducing generalization across species. Dorado, the only tool for plant 5mC detection on the R10.

Global, Regional, and National Burden of Trachoma: Cross-Country Inequalities, 1990-2021, with Projections to 2040.

Introduction: In this work, we aim to evaluate the trends and cross-country inequalities of the global trachoma burden from 1990 to 2021 and to project its burden to 2040.

Methods: This study was a systematic analysis of the Global Burden of Diseases Study (GBD) 2021. Estimates for the prevalence and disability-adjusted life-years (DALYs) of trachoma were extracted from the GBD 2021 database.

Diagnostic Challenges in Fuchs' Uveitis Syndrome in China: A Multi-Center Comparative Study of Three Criteria.

Objective: To evaluate and compare the diagnostic accuradcy of three Fuchs' Uveitis Syndrome (FUS) diagnostic criteria-La Hey Diagnostic Criteria (LHDC), the Standardization of Uveitis Nomenclature (SUN) Criteria, and Yang's Revised Diagnostic Criteria (RDC)-in the absence of a universally accepted gold standard.

Methods: A multicenter, case-control study was conducted with 673 patients (331 FUS and 342 non-FUS) from three tertiary eye centers in China. Sensitivity, specificity, and area under the curve (AUC) values were calculated and compared across the three criteria.

Reconstruction of diploid higher-order human 3D genome interactions from noisy Pore-C data using Dip3D.

Differential high-order chromatin interactions between homologous chromosomes affect many biological processes. Traditional chromatin conformation capture genome analysis methods mainly identify two-way interactions and cannot provide comprehensive haplotype information, especially for low-heterozygosity organisms such as human. Here, we present a pipeline of methods to delineate diploid high-order chromatin interactions from noisy Pore-C outputs.

The thylakoid protein BCM1 sequesters antennae protein CP24 and CP29 within the grana cores thereby reducing their exposure to degradation under heat stress.

Photosystem II (PSII) is one of the most thermosensitive components of photosynthetic apparatus in higher plants. Heat-inactivation of PSII may be followed by dissociation of antenna proteins, however, the fate and regulation mechanism of detached antenna proteins during this process remains unclear. Here, we investigate the regulation mechanism of two minor antenna proteins CP24 and CP29 during heat acclimation via the study on a thylakoid protein BCM1.

High performers in the shadow: The adverse effect of star employees on their peers.

Star employees are pivotal to organizational success and significantly influence their peers. Previous studies on this topic often explore the attributes of stars and nonstars in isolation. Using social comparison theory, our study posits that as employees' performance approaches that of star employees, nonstar employees become more likely to compare themselves with stars, thereby increasing their sense of psychological entitlement.

LcASR enhances tolerance to abiotic stress in Leymus chinensis and Arabidopsis thaliana by improving photosynthetic performance.

As a crucial forage grass, Leymus chinensis plays significant roles in soil and water conservation owing to its robust stress resistance. However, the underlying molecular mechanisms of its stress tolerance remain unclear. In this study, a novel gene, designated as LcASR (Abiotic Stress Resistance in Leymus chinensis), imparting resilience to both high light and drought, was identified.

Mechanisms of mitochondrial resilience in teleostean radial glia under hypoxic stress.

Radial glial cells (RGCs) are remarkable cells, essential for normal development of the vertebrate central nervous system. In teleost fishes, RGCs play a pivotal role in neurogenesis and regeneration of injured neurons and glia. RGCs also exhibit resilience to environmental stressors like hypoxia via metabolic adaptations.

A survey of knowledge, attitudes, and practices among paediatric intensive care unit nurses for preventing pressure injuries: An analysis of influencing factors.

To explore the knowledge, attitudes and practice (KAP) status of preventing pressure injury among clinical nurses working in paediatric ICU, and to examine factors affecting nurses' KAP. A questionnaire survey was conducted among 1906 paediatric ICU nurses in 18 children's hospitals by convenience sampling method. The survey tools were self-designed general data questionnaire, KAP questionnaire for the prevention of pressure injury and the influencing factors were analysed.

A novel function and mechanism of ischemia-induced retinal astrocyte-derived exosomes for RGC apoptosis of ischemic retinopathy.

Retinal ischemia is a common clinical event leading to retinal ganglion cell (RGC) death, resulting in irreversible vision loss. In the retina, glia-neuron communication is crucial for multiple functions and homeostasis. Extracellular vesicles, notably exosomes, play a critical role.

Caspase-8 promotes NLRP3 inflammasome activation mediates eye development defects in zebrafish larvae exposed to perfulorooctane sulfonate (PFOS).

Epidemiological evidence showed that serum high perfluorooctane sulfonate (PFOS) levels are associated with multiple eye related diseases, but the potential underlying molecular mechanisms remain poorly understood. Zebrafish and photoreceptor cell (661w) models were used to investigate the molecular mechanism of PFOS induced eye development defects. Our results showed a novel molecular mechanism of PFOS-induced inflammation response-mediated photoreceptor cell death associated with eye development defects.

Sprint-inspired One-on-One Post-Go-Live Training Session (Mini-Sprint) Improves Provider Electronic Health Record Efficiency and Satisfaction.

Background:  Inefficient electronic health record (EHR) usage increases the documentation burden on physicians and other providers, which increases cognitive load and contributes to provider burnout. Studies show that EHR efficiency sessions, optimization sprints, reduce burnout using a resource-intense five-person team. We implemented sprint-inspired one-on-one post-go-live efficiency training sessions (mini-sprints) as a more economical training option directed at providers.

Targeting inflammasomes and pyroptosis in retinal diseases-molecular mechanisms and future perspectives.

Retinal diseases encompass various conditions associated with sight-threatening immune responses and are leading causes of blindness worldwide. These diseases include age-related macular degeneration, diabetic retinopathy, glaucoma and uveitis. Emerging evidence underscores the vital role of the innate immune response in retinal diseases, beyond the previously emphasized T-cell-driven processes of the adaptive immune system.