Genistein in the Prevention and Treatment of Periodontitis: A Review.

Periodontitis is a chronic and progressive inflammatory disease of periodontal tissue whose characteristic pathological changes include the destruction of periodontal soft tissue and the absorption of alveolar bone. Genistein, an isoflavone compound derived from leguminous plants, has pharmacological effects such as anti-inflammatory, anti-oxidation, anti-bacteria, anti-osteoporosis, anti-tumor, and estrogen-like effects. In recent years, genistein has shown broad application potential in the prevention and treatment of periodontitis due to its wide range of biological activities.

Simultaneous knockout of multiple genes confers durable and broad-spectrum resistance to potyviruses in tobacco.

Unlabelled: Recessive resistance mediated by mutations in the eukaryotic translation initiation factor 4E (eIF4E), has proven effective against diverse potyviruses and is extensively utilized in breeding programs. However, the rise of resistance-breaking (RB) strains and emerging potyviral species necessitates the development of more durable and broad-spectrum resistance strategies. In this study, our field survey in Yunnan, China, identified potato virus Y (PVY) RB isolates, as well as the prevalence of tobacco vein banding mosaic virus (TVBMV) and chilli veinal mottle virus (ChiVMV), in tobacco carrying the recessive locus, which lacks the susceptibility gene, due to a chromosomal deletion.

Identification of a gene conferring broad-spectrum orthotospovirus resistance in Solanaceae.

Linkage drag can hinder the integration of resistance genes from wild crop relatives into breeding programs. We used a chromosome-scale genome assembly and a segregating population exceeding 160,000 plants to dissect the complex genetic architecture and overcome the tight linkage between resistance and deleterious loci to produce plants free from linkage drag. We cloned , encoding an immune receptor that confers broad-spectrum resistance to orthotospoviruses through the interaction of its carboxyl-terminal domain with an orthotospovirus-encoded protein.

Ischemia-free liver transplantation improves long-term outcomes in a 5-year follow-up study.

Background & Aims: Ischemia-free liver transplantation (IFLT) is a novel technique designed to avoid ischemia-reperfusion injury (IRI). Here, we report the first detailed 5-year follow-up outcomes.

Methods: We conducted a cohort study comparing long-term outcomes between IFLT and conventional liver transplantation (CLT) recipients of livers donated after brain death (DBD).

Genome-wide identification and characterization of NBS-LRR gene family in tobacco (Nicotiana benthamiana).

Nicotiana benthamiana is an important model plant widely employed in plant virology, which involves the nucleotide binding site leucine-rich repeat (NBS-LRR) genes in disease resistance. In this study, firstly, 156 NBS-LRR homologs were identified by HMMsearch and they were divided into three clades in the phylogenetic tree by Clustal W, containing 5 TNL-type, 25 CNL-type, 23 NL-type, 2 TN-type, 41 CN-type, and 60 N-type proteins. Further, conserved motifs and domain composition were detected by MEME, showing 10 conserved motifs dispersed throughout the protein sequences in both typical- and irregular- type NBS-LRRs.

Poly(3,4-ethylenedioxythiophene)-Coated Vanadium-Doped MnO Nanorods for High-Performance Flexible Aqueous Zinc-Ion Battery Cathode.

Manganese-based aqueous zinc-ion batteries (AZIBs) are considered promising cathode materials for large-scale energy storage applications due to their low cost and high safety. However, the primary constraints on achieving high specific capacity and cycling stability are the inherent low conductivity and suboptimal structural stability of the AZIB cathodes. Herein, we report a high-performance poly(3,4-ethylenedioxythiophene) (PEDOT)-coated vanadium-doped MnO nanorod (NR) electrode for AZIBs.

CD19CD73 B cells infiltration indicates poor prognosis and unfavorable responses to immunotherapy in gastric cancer.

Objectives: Cluster of Differentiation 73 (CD73) is expressed on immune cells and plays a significant role in tumor inhibition by suppressing antitumor immunity. The objectives of this study were to explore the expression and functional mechanisms of CD73 on B cells in patients with gastric cancer (GC).

Methods: The prognostic significance of CD19CD73 B cells was evaluated in 390 GC patients through dual immunohistochemistry staining.

Astaxanthin Alleviates Autoimmune Hepatitis by Modulating CD8 T Cells: Insights From Mass Cytometry and Single-Cell RNA Sequencing Analyses.

Astaxanthin (ASX) is an oxygen-containing non-vitamin A carotenoid pigment. However, the role of ASX in autoimmune hepatitis (AIH) remains unclear. In this study, a mouse model of AIH is established induced by concanavalin A (ConA).

Greatly Enhanced Thermoelectric Performance of Flexible CuS Composite Film on Nylon by Se Doping.

In this work, flexible CuS films on nylon membranes are prepared by combining a simple hydrothermal synthesis and vacuum filtration followed by hot pressing. The films consist of CuS and CuS two phases with grain sizes from nano to submicron. Doping Se on the S site not only increases the CuS content in the CuS to increase carrier concentration but also modifies electronic structure, thereby greatly improves the electrical properties of the CuS.

Electroacupuncture attenuates neuropathic pain via suppressing BIP-IRE-1α-mediated endoplasmic reticulum stress in the anterior cingulate cortex.

Studies have suggested that endoplasmic reticulum stress (ERS) is involved in neurological dysfunction and that electroacupuncture (EA) attenuates neuropathic pain (NP) via undefined pathways. However, the role of ERS in the anterior cingulate cortex (ACC) in NP and the effect of EA on ERS in the ACC have not yet been investigated. In this study, an NP model was established by chronic constriction injury (CCI) of the left sciatic nerve in rats, and mechanical and cold tests were used to evaluate behavioral hyperalgesia.

Comparative transcriptome analysis reveals nicotine metabolism is a critical component for enhancing stress response intensity of innate immunity system in tobacco.

The pyridine alkaloid nicotine acts as one of best-studied plant resistant traits in tobacco. Previous research has shown that and , acting as master regulators within the and locus, quantitatively contribute to nicotine accumulation levels in . Genome editing-created () and () double mutant provides an ideal platform for precisely dissecting the defensive role of nicotine and the connection between the nicotine biosynthetic pathway with other putative metabolic networks.

Establishment and application of Agrobacterium-delivered CRISPR/Cas9 system for wild tobacco () genome editing.

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR-Cas9) system has been widely applied in cultivated crops, but limited in their wild relatives. is a typical wild species of genus that is globally distributed as a horticultural plant and well-studied as a self-incompatibility model. It also has valuable genes for disease resistance and ornamental traits.

Exosomes in tumor-stroma crosstalk: Shaping the immune microenvironment in colorectal cancer.

Colorectal cancer (CRC) is a multifaceted disease characterized by a complex interaction between tumor cells and the surrounding microenvironment. Within this intricate landscape, exosomes have emerged as pivotal players in the tumor-stroma crosstalk, influencing the immune microenvironment of CRC. These nano-sized vesicles, secreted by both tumoral and stromal cells, serve as molecular transporters, delivering a heterogeneous mix of biomolecules such as RNAs, proteins, and lipids.

NtERF4 promotes the biosynthesis of chlorogenic acid and flavonoids by targeting PAL genes in Nicotiana tabacum.

Chlorogenic acid (CGA) and flavonoids are important secondary metabolites, which modulate plant growth and development, and contribute to plant resistance to various environmental stresses. ERF4 has been shown to be a repressor of anthocyanin accumulation in grape, but its full roles in regulating the biosynthesis of other phenylpropanoid compounds still needs to be further studied. In the present study, two NtERF4 genes were identified from N.

Characterization of the CMS genetic regulation through comparative complete mitochondrial genome sequencing in Nicotiana tabacum.

Mitochondrial genomes (mitogenomes) of flowering plants vary greatly in structure and size, which can lead to frequent gene mutation, rearrangement, or recombination, then result in the cytoplasmic male sterile (CMS) mutants. In tobacco (Nicotiana tabacum), suaCMS lines are widely used in heterosis breeding; however, the related genetic regulations are not very clear. In this study, the cytological observation indicated that the pollen abortion of tobacco suaCMS(HD) occurred at the very early stage of the stamen primordia differentiation.

High-Performance AgSe Film by a Microwave-Assisted Synthesis Method for Flexible Thermoelectric Generators.

Flexible AgSe thermoelectric (TE) films are promising for wearable applications near room temperature (RT). Herein, a AgSe film on a nylon membrane with high TE performance was fabricated by a facile method. First, AgSe powders were prepared by a microwave-assisted synthesis method using Ag nanowires as a template.

CRISPR/Cas9-mediated seamless gene replacement in protoplasts expands the resistance spectrum to TMV-U1 strain in regenerated Nicotiana tabacum.

CRISPR/Cas-based genome editing is now extensively used in plant breeding and continues to evolve. Most CRISPR/Cas current applications in plants focus on gene knock-outs; however, there is a pressing need for new methods to achieve more efficient delivery of CRISPR components and gene knock-ins to improve agronomic traits of crop cultivars. We report here a genome editing system that combines the advantages of protoplast technologies with recent CRISPR/Cas advances to achieve seamless large fragment insertions in the model Solanaceae plant Nicotiana tabacum.

NLRs derepress MED10b- and MED7-mediated repression of jasmonate-dependent transcription to activate immunity.

Plant intracellular nucleotide-binding domain, leucine-rich repeat-containing receptors (NLRs) activate a robust immune response upon detection of pathogen effectors. How NLRs induce downstream immune defense genes remains poorly understood. The Mediator complex plays a central role in transducing signals from gene-specific transcription factors to the transcription machinery for gene transcription/activation.

A randomized-controlled trial of ischemia-free liver transplantation for end-stage liver disease.

Background & Aims: Ischemia-reperfusion injury (IRI) has thus far been considered as an inevitable component of organ transplantation, compromising outcomes, and limiting organ availability. Ischemia-free organ transplantation is a novel approach designed to avoid IRI, with the potential to improve outcomes.

Methods: In this randomized-controlled clinical trial, recipients of livers from donors after brain death were randomly assigned to receive either an ischemia-free or a 'conventional' transplant.

Interspecies/Intergroup Complementation of Orthotospovirus Replication and Movement through Reverse Genetics Systems.

Orthotospoviruses, the plant-infecting bunyaviruses, cause serious diseases in agronomic crops and pose major threats to global food security. The family of contains more than 30 members that are classified into two geographic groups, American-type and Euro/Asian-type orthotospovirus. However, the genetic interaction between different species and the possibility, during mixed infections, for transcomplementation of gene functions by orthotospoviruses from different geographic groups remains underexplored.

Propofol inhibits cell apoptosis and inflammatory response in ox-LDL-induced human umbilical vein endothelial cells through the modulation of the circ_0003645/miR-149-3p/TRAF7 axis.

Background: Propofol is an anesthetic agent and can impede the progression of human diseases. Circular RNA (circRNA) circ_0003645 has been identified to promote the development of atherosclerosis (AS). This study aimed at the functional mechanism of propofol and circ_0003645 in AS.

PSD-95 in the anterior cingulate cortex contributes to neuropathic pain by interdependent activation with NR2B.

Studies suggest that the scaffolding protein, postsynaptic density protein-95 (PSD-95), is involved in multiple neurological dysfunctions. However, the role of PSD-95 in the anterior cingulate cortex (ACC) in neuropathic pain (NP) has not been investigated. The current study addressed the role of PSD-95 in the ACC in NP and its modulating profile with NMDA receptor subunit 2B (NR2B).