Soil organic carbon distribution and multi-scale drivers in semi-arid alpine Regions: Implications for carbon storage function stability.

The spatial distribution pattern of soil organic carbon (SOC) directly influences carbon sequestration and climate regulation potential. However, in semi-arid alpine mountain regions, the spatial heterogeneity of SOC and its underlying driving mechanisms remain insufficiently investigated. Furthermore, empirical evidence for the quantitative assessment of soil carbon storage capacity across different vegetation units under natural conditions is still lacking.

Screening and Predicting Multi-Omics T-ALL Core Genes Based on PU Learning.

T-cell acute lymphoblastic leukemia (T-ALL) is a malignant neoplastic disease. Accurate identification of core genes helps to explore the pathogenesis of T-ALL and develop relevant targeted drugs. In this paper, we first screened the RNA-seq, CTCF ChIP-seq and DNA methylation datasets of T-ALL for intersecting differentially expressed genes (DEGs) using bioinformatics software.

Technetium-99m-Labeled Fibroblast Activation Protein-Targeted Nanobody Radiotracer: from Preclinical Development to Clinical Translation in Cancer Imaging.

Fibroblast activation protein (FAP) is a highly expressed marker in cancer-associated fibroblasts (CAFs) across various epithelial cancers, making it an attractive target for diagnostic imaging. To address the limitations of existing FAP-targeted radiopharmaceuticals, such as poor tumor retention and off-target uptake, this study aimed to establish a comprehensive FAP nanobody library. Through systematic screening, we sought to identify a nanobody with cross-reactivity to both human and murine FAP, optimized for technetium-99m (Tc) labeling, and suitable for single-photon emission computed tomography (SPECT) imaging.

A Novel PSMA/FAP-Specific Radiotracer Tc-HFaPSMA for Diagnosis of PSMA-Negative Prostate Cancer: From Preclinical Validation to Clinical Translation.

In this study, we reported a bispecific radiotracer [Tc]Tc-HFaPSMA to improve the diagnostic sensitivity of prostate cancer (PCa). [Tc]Tc-HFaPSMA was prepared by Tc radiolabeling of a HYNIC-conjugated bispecific biomolecule HFaPSMA, which combines a PSMA-targeting motif (PhPSMA), a FAP-targeting ligand (GFAPI), a symmetric PEG-N-bis(PEG-acid) linker, and a chelator (HYNIC). Preclinical studies showed faster clearance and higher tumor uptake than that of its corresponding single-target radiotracers ([Tc]Tc-HPSMA and [Tc]Tc-HGFAPI).

Platinum single atom anchoring on nano-MOF/tubular carbon nitride enabled nitrogen activation and multiple electron transfer for photocatalytic nitrogen fixation.

Photocatalytic nitrogen fixation offers a sustainable pathway for green ammonia synthesis under mild conditions, yet its practical application is hindered by the inefficient charge separation and insufficient active sites of conventional photocatalysts. Herein, we present a rationally engineered heterostructure comprising Pt single atoms (PtSAs) on nano metal-organic frameworks (MOF-74)/hollow double-shelled tubular CN (TCN), which addresses these limitations through synergistic effects. The Z-scheme heterojunction formed between MOF-74 and TCN establishes a built-in electric field, facilitating directional electron transfer from MOF-74 to PtSAs-modified TCN.

Impact of kyphosis exceeding 30° on lumbar spine in patients with post-traumatic kyphosis.

This study investigated the impact of post-traumatic kyphosis (PTK) on the lumbar spine using a local Cobb angle of 30° as the cutoff point. The measurements included the local Cobb angle, lumbar lordosis angle (L1-L5), intervertebral space angle (IVSA), lumbar disc degeneration, and posterior wall height loss (PWHL). Patient characteristics such as sex, age, disease duration, American Spinal Injury Association (ASIA) grade, fracture site, bone mineral density (BMD), Oswestry Disability Index (ODI), and visual analogue scale (VAS) score were also recorded.

Preclinical Evaluation of a Nanobody-based SPECT Radiotracer Tc-AFN with Clinical Potential for Noninvasive Monitoring of Fibroblast Activation after Myocardial Infarction.

Purpose To explore the feasibility of a new technetium 99m-labeled nanobody (Tc-antifibroblast activation protein nanobody [AFN]) with high affinity to fibroblast activation protein for noninvasive monitoring of fibroblast activation after myocardial infarction. Materials and Methods Tc-AFN was prepared by site-specific labeling. Dynamic SPECT/CT imaging from 0.

Resistance to iron deficiency is mediated through rhizosphere acidification and ferric chelate reductase activity in Pyrus betulaefolia.

Iron (Fe) deficiency stress seriously impacts the yield and quality of pear fruit. Nevertheless, the mechanism of Fe absorption in pears (Pyrus betulaefolia) remains unclear. ARFs are a class of transcription factors that are widely involved in plant stress response.

High precision control moment gyroscope fault diagnosis via joint attention mechanism.

The fault of one of the key systems in artificial satellites, the Control Moment Gyroscope (CMG), can lead to significant economic losses and irreparable consequences. Therefore, it is crucial to diagnose its faults promptly. Traditional fault diagnosis methods, however, face challenges such as local feature traps and difficulty in feature extraction when dealing with CMG vibration signals, making it hard to meet the requirements for accuracy and robustness.

Integrative physiological and transcriptomic analysis provides insights on the molecular basis of ABA-enhanced drought tolerance in pear (Pyrus betulaefolia).

Background: Drought stress could suppress the carbon assimilation and limit nutrient uptake of pear plants, thus affecting their growth and severely impacting the quality and yield of pear fruit. ABA is a stress hormone and is reported to alleviate drought stress in numerous plants. However, whether and how ABA functions in the drought responses of pear plants is yet explored.

Comprehensive Genomic Analysis of Trihelix Transcription Factor Genes and Their Expression Underlying Abiotic Stress in Euphrates Poplar ().

Trihelix transcription factors (TTFs) are light-sensitive proteins characterized by a triple-helix structure that play a crucial role in regulating plant growth and development, especially in response to abiotic stressors, such as drought and salinity. This intriguing family of proteins has been the focus of extensive functional studies across various plant species. Despite their recognized significance, the trihelix family in has not been thoroughly explored, warranting more attention.

Efficacy of radiolabelled PD-L1-targeted nanobody in predicting and evaluating the combined immunotherapy and chemotherapy for resectable non-small cell lung cancer.

Background: This study aimed to assess the predictive and evaluative value of PD-L1 targeted Ga-THP-APN09 PET/CT in the neoadjuvant immunotherapy combined with chemotherapy for resectable non-small cell lung cancer (NSCLC), and to explore its potential in indicating immunotherapy-related adverse effects (irAEs).

Methods: Fifty patients with resectable NSCLC enrolled in this prospective study underwent baseline Ga-THP-APN09 PET/CT and F-FDG PET/CT, with follow-up F-FDG PET/CT conducted, additionally, 36 patients received follow-up Ga-THP-APN09 PET/CT. Surgery was performed following 2-4 cycles of toripalimab combined with chemotherapy if R0 resection was feasible.

Development and First-in-Human evaluation of a Site-Specific [F]-Labeled PD-L1 nanobody PET radiotracer for noninvasive imaging in NSCLC.

Immunohistochemistry (IHC) for PD-L1 detection is limited by its invasiveness and heterogeneity of tumors. To address these challenges, a new PD-L1-targeted nanobody-based immune-PET radiotracer [F]AlF-APN09 was developed using the site-specific radiolabeling method with the complexing agent (Mal-RESCA) under mild conditions. [F]AlF-APN09 was prepared at room temperature (pH 4.

CD38-specific immunoPET imaging for multiple myeloma diagnosis and therapeutic monitoring: preclinical and first-in-human studies.

Purpose: CD38 is a glycoprotein highly specific to multiple myeloma (MM). Therapeutics using antibodies targeting CD38 have shown promising efficacy. However, the efficient stratification of patients who may benefit from daratumumab (Dara) therapy and timely monitoring of therapeutic responses remain significant clinical challenges.

Gene Family Identification in Chinese White Pear () and the Functional Analysis of in Fe Deficiency Responses in Tomato.

Iron (Fe) deficiency poses a major threat to pear ( spp.) fruit yield and quality. The () plays a vital part in plant stress responses.

Functional Analysis of CPSF30 in Using RNA Interference Reveals Its Essential Role in Development and Survival.

The brown planthopper () is a major pest threatening global rice production, significantly reducing yields annually. As increasingly develops resistance to conventional control methods, such as chemical pesticides, there is an urgent need for innovative and sustainable pest management strategies. Cleavage and Polyadenylation Specificity Factor 30 (CPSF30) is a key protein involved in mRNA 3' end processing, yet its function in remains poorly understood.

Nuclear imaging of PD-L1 expression promotes the synergistic antitumor efficacy of targeted radionuclide therapy and immune checkpoint blockade.

Purpose: In order to maximize synergistic effect of targeted radionuclide therapy (TRT) and immune checkpoint blockade (ICB) as well as reduce the toxicity, we pioneered a strategy guided by PD-L1-targeted nuclear medicine imaging for the combination of TRT and ICB towards precision cancer therapy.

Methods: As a novel targeted radiotherapeutic agent, Lu-AB-3PRGD targeting integrin αβ was developed to achieve sustained antitumor effect by introducing an albumin binder (AB) into the structure of 3PRGD. The Lu-AB-3PRGD TRT as well as different types of combination therapies of Lu-AB-3PRGD TRT and anti-PD-L1 ICB were performed in animal models.

Nondestructive Detection of Corky Disease in Symptomless 'Akizuki' Pears via Raman Spectroscopy.

'Akizuki' pear ( Nakai) corky disease is a physiological disease that strongly affects the fruit quality of 'Akizuki' pear and its economic value. In this study, Raman spectroscopy was employed to develop an early diagnosis model by integrating support vector machine (SVM), random forest (RF), gradient boosting decision tree (GBDT), extreme gradient boosting (XGBoost), and convolutional neural network (CNN) modeling techniques. The effects of various pretreatment methods and combinations of methods on modeling results were studied.

Genome-Wide Analysis of Aquaporins Gene Family in and Its Expression Patterns in Response to Drought, Salt Stress, and Phytohormones.

Aquaporins (AQPs) play an essential role in membrane water transport during plant responses to water stresses centered on conventional upstream signals. Phytohormones (PHs) regulate plant growth and yield, working with transcription factors to help plants withstand environmental challenges and regulate physiological and chemical processes. The AQP gene family is important, so researchers have studied its function and regulatory system in numerous species.

Transcriptome Analysis and Identification of Genes Associated with Cotton Seed Size.

Cotton seeds, as the main by-product of cotton, are not only an important raw material for edible oil and feed but also a source of biofuel. The quality of cotton seeds directly affects cotton planting and is closely related to the yield and fiber quality. However, the molecular mechanism governing cotton seed size remains largely unexplored.

Assessment of the Restoration Potential of Forest Vegetation Coverage in the Alxa Desert Region of China.

To scientifically evaluate the sustainability of tree planting and afforestation in the Alxa Desert region, this study, grounded in the principles of water balance within the natural water cycle, employed multi-source remote sensing products and ground-based measurements to construct a quantitative response relationship model. This model links evapotranspiration (ET) with meteorological variables and the Enhanced Vegetation Index (EVI). Furthermore, the study estimated the recovery thresholds and potential of forest and grassland vegetation coverage in the Alxa Desert region under various precipitation scenarios.

Claudin18.2-Targeted SPECT/CT Imaging for Gastric Cancer: Preclinical Evaluation and Clinical Translation of the Tc-Labeled Nanobody (PHG102) Radiotracer.

Claudin18.2 (CLDN18.2) has emerged as a significant target in the treatment of advanced gastric cancer.

Deciphering the dynamic expression network of fiber elongation and the functional role of the GhTUB5 gene for fiber length in cotton based on an introgression population of upland cotton.

Introduction: Interspecific introgression between Gossypium hirsutum and G. barbadense allows breeding cotton with outstanding fiber length (FL). However, the dynamic gene regulatory network of FL-related genes has not been characterized, and the functional mechanism through which the hub gene GhTUB5 mediates fiber elongation has yet to be determined.

Study on full-scale pores characterization and heterogeneity of coal based on low-temperature nitrogen adsorption and low-field nuclear magnetic resonance experiments.

The characteristics and heterogeneity of coal pores are crucial for understanding the production mechanism of coalbed methane (CBM). In this study, coal samples with varying degrees of metamorphism (0.58%  ≤ R, ≤ 3.