Complete mitochondrial DNA sequence analysis in patients with major depressive disorder.

Objective: This study aimed to identify potential mutations associated with major depressive disorder (MDD) and evaluate disease-associated risk factors.

Methods: Total genomic DNAwas extracted from the participants' blood samples, and the complete mitochondrial genome wasamplified by PCR, purified, and sequenced. Mutation burden analysis and functional mutation analysis was performed, including total mutation counts, highly conserved mutations (Conservation Index >75 %), and structurally disruptive mutations.

A Generalized and Interpretable Multi-Label Multi-Disease Screening System for Ocular Anterior Segment Disease Detection.

Objective: To develop and validate a multi-label, multi-disease, well-generalized, and interpretable screening system applied to the detection of common ocular anterior segment diseases based on ocular surface slit-lamp images.

Design: A multicenter artificial intelligence diagnostic study.

Participants: A total of 1990 patients were randomly selected from 2 medical centers: the Second Affiliated Hospital of Zhejiang University and the Affiliated People's Hospital of Ningbo University, between November 2016 and March 2022.

Diagnostic and management strategies for retroperitoneal leiomyosarcoma invading the inferior vena cava: a case report.

Background: Retroperitoneal leiomyosarcoma represents a poor-prognosis malignancy for which current clinical treatment options remain limited. This study may further help bridge the gap in available therapeutic modalities.

Case Presentation: This case report describes a 75-year-old female patient who was admitted to the hospital with right lower abdominal pain and imaging studies suggesting a retroperitoneal mass and consideration of leiomyosarcoma.

Comprehensive risk stratification for adult T-cell lymphoblastic lymphoma undergoing allogeneic haematopoietic stem cell transplantation.

Relapse remains the primary cause of transplant failure in T-cell lymphoblastic lymphoma (T-LBL) after allogeneic haematopoietic stem cell transplantation (allo-HSCT), yet prognostic data are scarce. We conducted a multicentre retrospective study of 104 adult T-LBL patients in complete metabolic remission (CMR) undergoing allo-HSCT. Pretransplant Deauville score (DS) was evaluated, and a risk model integrating DS, immunophenotype and donor type was developed.

Prognostic value of Day 14 measurable residual disease in acute myeloid leukemia treated with venetoclax and azacitidine.

Background: Venetoclax (VEN) in combination with azacitidine (AZA) (VEN-AZA) is used to treat acute myeloid leukemia (AML) in patients who are not candidates for intensive chemotherapy but research on prognostic factors remains limited.

Methods: Measurable residual disease (MRD) by multiparametric flow cytometry in AML is important but there is limited evidence of the clinical utility of monitoring MRD in patients treated with VEN-AZA. Herein, a total of 75 patients newly diagnosed with AML treated with VEN-AZA were retrospectively analyzed to examine the role and timing of MRD to predict survival.

Fucoxanthin protects retinal ganglion cells and regulates Parkin-mediated mitophagy in experimental glaucoma.

Objective: Glaucoma is a common neurodegenerative disease resulting in irreversible blindness. This study investigates whether fucoxanthin can safeguard retinal ganglion cells (RGCs) by modulating Parkin-mediated mitophagy in experimental glaucoma.

Methods: An experimental glaucomatous model was induced in Sprague-Dawley rats via translimbal laser photocoagulation.

regulates cell proliferation, migration, apoptosis and ferroptosis in gastric cancer.

Background: Gastric cancer (GC) has a high prevalence and mortality overall. is associated with abnormal centrosome amplification, DNA damage and increased apoptosis. To date, little is known about the function and mechanism of in GC.

Venetoclax combined with azacitidine in elderly acute myeloid leukemia: A retrospective comparison of 14-day vs 28-day dosing regimens.

However, this study has several limitations that must be acknowledged. First, the non-randomized allocation of treatment duration introduces potential selection bias, particularly as frailer patients were more likely to receive shorter therapeutic cycles, which may have confounded outcome assessments. Background: Although the standard 28-day venetoclax (VEN) regimen combined with azacitidine (AZA) improves outcomes in elderly patients with acute myeloid leukemia, emerging evidence suggests that shorter VEN cycles may maintain efficacy with enhanced safety.

Comprehensive omics analysis of type 2 diabetes mellitus and cardioembolic stroke provides new biological insights and therapeutic targets.

The objective of this study is to investigate the shared genes that are differentially expressed (DEGs) between CES and T2DM, as well as uncover the hidden molecular mechanisms involved. We retrieved the gene expression profiles for CES (GSE58294) and T2DM (GSE25724) from Gene Expression Omnibus (GEO) database. We then per formed 5 analyses: Identify the overlapping DEGs between CES and T2DM, correlation analysis of hub genes; transcriptional regulation analysis of hub genes; single-cell sequencing analysis and potential therapeutic drug prediction.

Urethral foreign body insertion for symptom relief: a case report.

Background: Urethral foreign body insertion is a rare and challenging clinical condition often associated with significant morbidity. Previous reports have highlighted motivations such as sexual gratification or psychiatric disorders. However, this case report uniquely focuses on a less-discussed motivation: the alleviation of physical discomfort from prior trauma.

HERV-K TM Subunit Elicits CD8 T Cell Anergy and Tumor Immune Evasion via Targeting CD3 Coreceptor ε in AML and PDAC.

CD8 T cell anergy is a critical driver of cancer immune evasion, but the underlying causes and mechanisms remain elusive. Here, the functional human endogenous retroviruses-K envelope (HERV-K Env) subunit transmembrane (K-TM) is identified as a potent viral immune checkpoint that induces CD8 T cell anergy and elicits immune evasion in acute myeloid leukemia (AML) and pancreatic duct adenocarcinoma (PDAC). K-TM subunits are highly expressed in CD8 T cells and enriched in sera of cancer patients.

Association between real-time shear wave elastography findings and HER-2 expression in breast cancer.

Background: Human epidermal growth factor receptor-2 (HER-2) overexpressing breast cancer is associated with more aggressive tumor characteristics and poorer clinical outcomes. It is important to determine HER-2 status of breast cancer for better treatment planning. This study aims to explore the correlation between real-time shear wave elastography (SWE) and HER-2 expression in breast cancer.

Immunogenic cell death-related genes as prognostic biomarkers and therapeutic insights in uterine corpus endometrial carcinoma: an integrative bioinformatics analysis.

Introduction: Immunogenic cell death (ICD) is the phenomenon in which tumor cells undergo the transition from a non-immunogenic state to an immunogenic state upon their demise as a result of external stimuli. While ICD systems have been widely adopted in oncological research, their specific utilization for Uterine Corpus Endometrial Carcinoma (UCEC) investigations has received comparatively little attention.

Methods: The ICD score was assessed using single-sample gene set enrichment analysis (ssGSEA).

Macular vascular density changes in different stages of chronic primary angle-closure glaucoma.

Objective: This study aims to investigate differences in macular vascular density (MVD) between individuals with chronic primary angle-closure glaucoma (CPACG) and healthy controls, as well as to evaluate cross-sectional changes in MVD at various stages of CPACG.

Method: This is a retrospective study based on the epidemiological survey of eye diseases in the local community, including 47 eyes of CPACG subjects (20 eyes at the early stage and 27 eyes at the middle-to-severe stages). All subjects underwent optical coherence tomography angiography (OCTA) imaging to detect MVD, as well as macular retinal nerve fiber layer (RNFL) and ganglion cell layer (GCL) thickness.

PCA-assisted label-free surface-enhanced Raman scattering detection of exosomes in prostate cancer at different stages based on dual-layer membrane g-CN@MXene SERS platform prepared by electrodeposition.

Accurate differentiation of prostate cancer at various stages provides a reliable basis for clinical decision-making in cancer treatment. However, there is still no effective protocol to enable the rapid and accurate identification of cancer progression. With the aim to improve the diagnostic efficiency of prostate cancer at different stages, we systematically analyzed exosomes derived from prostate cancer at different stages of disease progression by integrating surface-enhanced Raman spectroscopy (SERS) with principal component analysis (PCA) in this work.

Diagnostic efficacy of Mycoplasma pneumoniae antibody and procalcitonin in children with Mycoplasma pneumonia.

Background: The prognosis of children with Mycoplasma pneumoniae pneumonia (MPP) is significantly impacted by the possibility of a missed or delayed diagnosis during the early stages of the illness. In this study, we assessed the potential of utilizing MP antibody and procalcitonin levels as diagnostic markers in pediatric patients with MPP, and their correlation with drug-resistance gene mutations.

Methods: A retrospective analysis was conducted on 80 hospitalized children with MPP confirmed by 23 S PCR and 80 healthy controls, focusing on serum MP antibody and procalcitonin (PCT) levels.

ESO-Shanghai 20: phase III clinical trial of anti-PD-1 therapy with local intervention for oligometastatic ESCC.

NCT06190782 (ClinicalTrials.gov).

Relationship between cognitive functions and serum neurotrophic factor levels in long-term hospitalized male patients with schizophrenia.

Objective: This study explored the changes in serum neurotrophic factor levels, the status of cognitive function, event-related potential P300, and the relationships between serum neurotrophic factor levels and cognitive functions, as well as event-related potential P300 in male schizophrenia patients with long-term hospitalization.

Methods: A total of 82 male schizophrenia patients with long-term hospitalization and 52 healthy controls were recruited. Cognitive functions were evaluated in all participants, including verbal fluency function, attention function, executive function, and spatial function.

Orthokeratology, 0.04% Atropine, and 0.01% Atropine for Myopia Control: A Randomized Clinical Trial.

Importance: Investigating the antimyopia efficacy and safety of orthokeratology and varying atropine doses in specific age groups could optimize personalized treatment choices.

Objective: To evaluate the efficacy and safety of orthokeratology, 0.04% atropine, and 0.

Eosinophilic gastroenteritis with negative endoscopic biopsy and no peripheral eosinophilia: A case report.

Rationale: Eosinophilic gastroenteritis (EoGE) is a rare inflammatory disease that can affect the entire gastrointestinal tract. Klein et al classified EoGE into 3 distinct subtypes according to the depth of eosinophilic infiltration: mucosal, muscular, and serosal.

Patient Concerns: We herein report a case of a 29-year-old woman who presented with abdominal pain, nausea, and vomiting, with no history of adverse reactions to any allergens.