RNA-binding proteins mediate the maturation of chromatin topology during differentiation.

Topologically associating domains (TADs) and chromatin architectural loops impact promoter-enhancer interactions, with CCCTC-binding factor (CTCF) defining TAD borders and loop anchors. TAD boundaries and loops progressively strengthen upon embryonic stem (ES) cell differentiation, underscoring the importance of chromatin topology in ontogeny. However, the mechanisms driving this process remain unclear.

Climate change, health, and wearable biosensors: Harnessing emerging technologies to bridge environmental exposures and physiological responses.

Climate change poses a growing threat to human health, increasing exposure to extreme environmental conditions. Wearable biosensors provide real-time monitoring of physiological responses to heat stress, including cardiovascular strain, thermoregulatory disruptions, sleep disturbances, and biomarkers of heat-related illnesses. These devices also assess behavioural adaptations, such as reduced physical activity, offering insights into physiological resilience and susceptibility.

Temporary silicone oil tamponade for persistent macular holes: a multicentre study.

Background: Persistent full-thickness macular holes (FTMHs) following primary surgery represent a therapeutic challenge. Various surgical treatment approaches have been proposed. This study evaluates anatomical and functional outcomes of a temporary silicone oil tamponade in persistent FTMH in a larger cohort.

Refractive Outcomes Using Simulated Keratometry Versus Keratometry From an Optical Biometer.

Purpose: To compare refractive prediction accuracy using simulated keratometry (SimK) measurements obtained from a Scheimpflug tomographer (Pentacam AXL, Oculus) versus keratometry (K) measurements obtained from an optical biometer utilizing telecentric keratometry (IOLMaster 700 (IOLM700), Carl Zeiss Meditec AG) applied to modern IOL power calculation formulas.

Design: Retrospective accuracy and validity analysis METHODS: Setting: Private practice center STUDY POPULATION: Five hundred eighty-nine eyes with preoperative SimK and K measurements undergoing phacoemulsification and implantation of monofocal IOL (Clareon SY60WF IOL, Alcon Laboratories, Inc.).

Hydroxychloroquine Toxicity with Short Duration of Hydroxychloroquine Use and Unilateral Bull's Eye Maculopathy.

Purpose: To report the examination and multimodal imaging findings of a patient with unilateral bull's eye maculopathy.

Methods: A retrospective chart review of a 77-year-old patient with unilateral bull's eye maculopathy who presented to a tertiary retinal practice was performed. The patient's history, visual acuity, examination and multimodal imaging findings over five years of follow-up were described.

Reimagining Acute Diabetes Care: Insights From the Victorian Virtual Emergency Department.

Objective: New approaches to diabetes care are needed to better identify and manage diabetes emergencies outside of hospitals.

Research Design And Methods: A descriptive analysis of routinely collected data was conducted using data from the Victorian Virtual Emergency Department (VVED) Diabetes, a telehealth service that provides emergency care, including ketone testing by ambulance personnel, for patients across Victoria, Australia, experiencing non-life-threatening diabetes-related emergencies.

Results: Between July and December 2024, VVED Diabetes managed 868 diabetes-related emergencies, with 82.

Outcomes and complications among nonagenarians undergoing cardiac surgery: A scoping review.

Introduction: This review was aimed at understanding the scope of evidence regarding outcomes and complications in nonagenarians (90-99 years of age) undergoing open cardiac surgery.

Methods: The review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) Extension for Scoping Review Protocol guidelines. A search of three databases, MEDLINE, EMBASE, and the Cochrane Central Register of Controlled Trials, identified articles pertaining to nonagenarians undergoing various open cardiac surgical procedures.

The barriers for uptake of artificial intelligence in hepatology and how to overcome them.

Artificial intelligence (AI) methods in hepatology have proliferated since the mid-2010s, with numerous publications and some regulatory approvals. Yet, adoption of AI methods in real-world clinical practice and clinical research remains limited. Despite clear benefits of using AI to analyze complex data types in hepatology, such as histopathology, radiology images, multi-omics and more recently, natural language patient data, there are still substantial barriers and challenges to its integration into routine clinical workflows.

Cost-Effectiveness of In-Bed Cycling and Routine Physiotherapy for Patients Receiving Mechanical Ventilation.

Importance: The cost-effectiveness of adding early in-bed cycling to usual physiotherapy among adults receiving mechanical ventilation in the intensive care unit (ICU) compared with usual physiotherapy alone is unknown.

Objective: To evaluate the cost-effectiveness of in-bed cycling plus usual physiotherapy compared with usual therapy alone in the Critical Care Cycling to Improve Lower Extremity Strength (CYCLE) randomized clinical trial.

Design, Setting, And Participants: This trial-based economic evaluation with a 90-day time horizon compared early cycling plus usual physiotherapy vs usual physiotherapy alone from a societal perspective.

GEMsembler: consensus model assembly and structural comparison of genome-scale metabolic models across tools improve functional performance.

Genome-scale metabolic models (GEMs) are widely used in systems biology to investigate metabolism and predict perturbation responses. Automatic GEM reconstruction tools generate GEMs with different properties and predictive capacities for the same organism. Since different models can excel at different tasks, combining them can increase metabolic network certainty and enhance model performance.

A Case Series of Young People Receiving Adjunctive Immunotherapy for Neuroimmune-Mediated Major Mood or Psychotic Syndromes.

Background: Neuroimmune processes are often implicated in young people with atypical neuropsychiatric disorders, yet treatment implications remain controversial. This case series details young people with primary psychiatric disorders who received adjunctive immunotherapy after thorough investigation and extensive conventional treatments.

Methods: We evaluated 45 individuals (93% female, ages 12-30 years) with atypical psychiatric presentations suggesting potential neuroimmune involvement.

Adhesion on Hydrophobin Coatings: Adhesion Forces and the Influence of Surface Charge.

() is one of the bacterial species capable of forming multilayered biofilms on implants. Such biofilms formed on implanted medical devices often require the removal of the implant in order to avoid sepsis or, in the worst case, even the death of the patient. To address the problem of unwanted biofilm formation, its first step, i.

Corticotropin-Releasing Hormone in Melanoma and Nonmelanoma Skin Cancer.

Corticotropin-releasing hormone (CRH) regulates immunological and cellular processes. Recently, CRH is expressed in skin cancers, where its expression appears to correlate with the degree of malignancy. This study correlates CRH expression in melanoma metastases with patient survival and compares the intensity of CRH expression in melanoma to that in less aggressive skin cancer entities.

Bariatric Surgery in Patients With Prader-Willi Syndrome.

Prader-Willi Syndrome (PWS) is a genetic disorder characterized by insatiable hyperphagia, resulting in severe, early-onset obesity that is often refractory to conventional management. The associated comorbidities and reduced life expectancy in PWS present a significant therapeutic challenge. This review synthesizes the existing literature on the controversial role, outcomes, and complexities of bariatric surgery in patients with PWS.

Isocitrate dehydrogenase mutation and microenvironment in gliomas: do immunotherapy approaches matter?

Purpose Of Review: Gliomas with mutations in the gene for isocitrate dehydrogenase (IDH) display a unique immune microenvironment that is distinct from IDH-wildtype gliomas. This unique immune microenvironment is shaped by 2-hydroxyglutarate (2-HG), an oncometabolite produced by mutant IDH. These features provide an opportunity to develop and test targeted immunotherapies for IDH-mutant gliomas.

Food, Built and Socio-Economic Environments and Maternal-Infant Outcomes: A Geospatial and Structural Equation Modelling of 163 000 Births.

Objectives: To examine the combined influence of food environment, built environment, socio-economic status and individual factors (maternal age, parity, smoking status and need for an interpreter) on maternal overweight, gestational diabetes mellitus (GDM) and large-for-gestational age (LGA) births in Australia.

Design: Retrospective cohort study.

Setting: Melbourne, Australia.

Oleandrin-mediated suppression of MELK induces apoptosis, autophagy, and ferroptosis in human non-small cell lung cancer cells.

Background: Non-small-cell lung cancer NSCLC is the major diagnosed type of lung cancers in the USA and Europe. It is generally related to poor prognosis and low rates of survival. Oleandrin is a cardiac glycoside occurring naturally in Nerium oleander (Apocynaceae).

Chronic kidney disease and incident cancer risk: an individual participant data meta-analysis.

Background: Studies examining the association of chronic kidney disease (CKD) with cancer risk have demonstrated conflicting results.

Methods: This was an individual participant data meta-analysis including 54 international cohorts contributing to the CKD Prognosis Consortium. Included cohorts had data on albuminuria [urine albumin-to-creatinine ratio (ACR)], estimated glomerular filtration rate (eGFR), overall and site-specific cancer incidence, and established risk factors for cancer.

Single-step, chloroform-free Extraction of Lysophosphatidylcholine from Plasma for Cancer Biomarker Analysis.

Lysophosphatidylcholine (LPC) has been the subject of research for many years, but its role in lipid turnover is still not fully understood, neither its role in cancer development and progression. A crucial aspect in LPC research is its efficient and fast extraction from plasma and tissues to use LPC as a biomarker in clinical settings. The extraction methods commonly in use like Bligh & Dyer require the use of toxic halogenated solvents and are time consuming due to multiple extraction steps and subsequent solvent evaporation.

Predicting Treatment Response in Retinal Vein Occlusions Using Baseline Optical Coherence Tomography Biomarkers: A Systematic Review.

This systematic review examines the prognostic value of baseline optical coherence tomography (OCT) biomarkers in predicting visual acuity (VA) outcomes for eyes with macular edema secondary to retinal vein occlusions (RVO) treated with anti-VEGF therapies, steroids, laser photocoagulation, or combination treatments. VA predictions at 6, 12, and 24 months post-treatment were assessed using a narrative synthesis approach and vote counting based on effect direction relative to a minimal clinically important difference. Certainty of evidence was evaluated using GRADE guidelines.

HT SpaceM: A high-throughput and reproducible method for small-molecule single-cell metabolomics.

Single-cell metabolomics (SCM) promises to reveal metabolism in its complexity and heterogeneity, yet current methods struggle with detecting small-molecule metabolites, throughput, and reproducibility. Addressing these gaps, we developed HT SpaceM, a high-throughput SCM method combining cell preparation on custom glass slides, small-molecule matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (MS), and batch processing. We propose a unified framework covering quality control, characterization, structural validation, and differential and functional analyses.