Network pharmacology prediction and experimental validation to explore the potential mechanism of Danggui Shaoyao San against Acute Kidney Injury.

Ethnopharmacological Relevance: Acute kidney injury (AKI) is a growing worldwide health concern. Danggui Shaoyao San (DGSYS) was an frequently-used representative prescription to "promote blood and water and harmonize the body" in traditional Chinese medicine, and its underlying mechanism against AKI remains to be elucidated.

Aim Of The Study: To investigate the protective effect and potential molecular mechanism of DGSYS in alleviating AKI by network pharmacology and experiment validation.

The Correlation and Clinicopathological Significance of TNFAIP8L3 and RAC1 Expression in Lung Adenocarcinoma.

Lung adenocarcinoma (LUAD) remains one of the leading causes of cancer-related mortality worldwide. However, the expression and role of TIPE3 and RAC1 in LUAD are not well characterized. This study aimed to investigate the expression and clinicopathological significance of TNFAIP8L3 (TIPE3) and RAC1 in LUAD, as well as the relationship between these two proteins.

Efficacy and Safety of Entecavir, Tenofovir Disoproxil Fumarate, and Tenofovir Alafenamide Fumarate in Treating Acute-on-Chronic Liver Failure with Hepatitis B Virus: A Network Meta-analysis.

Introduction: Oral nucleos(t)ide analogues (NAs) are widely used in managing hepatitis B virus-associated acute-on-chronic liver failure (HBV-ACLF). Among first-line therapies, entecavir (ETV), tenofovir disoproxil fumarate (TDF), and tenofovir alafenamide (TAF) are commonly prescribed. However, their comparative efficacy and safety remain unclear in HBV-ACLF.

Drug-tolerant persister cell in cancer: reversibility, microenvironmental interplay, and therapeutic strategies.

Drug-tolerant persister (DTP) cells are a subpopulation of cancer cells capable of surviving therapeutic stress through reversible, non-genetic adaptations. These cells contribute to minimal residual disease and eventual tumor relapse. Understanding the mechanisms that govern the entry into and exit from the DTP state-such as epigenetic remodeling, metabolic rewiring, and transcriptional plasticity-reveals actionable vulnerabilities.

The Mediation of Circulating Inflammatory Proteins in the Causal Pathway From Immune Cells to Delirium.

Objective: Observational research indicates that immune cells, in conjunction with circulating inflammatory proteins, could serve two functions in the development of delirium, despite the fact that the exact mechanisms remain ambiguous. The objective of this research is to identify the specific pathways through which immune cells trigger delirium and to evaluate the possible function of circulating inflammatory proteins as intermediaries.

Methods: A two-sample Mendelian randomization (MR) analysis was conducted using summary-level data from genome-wide association studies, which included 731 immune cells, 91 inflammatory proteins, and their association with delirium.

Danhong Injection targets CaMKII through Dihydrotanshinone I to alleviate cardiomyocyte death and inflammation in viral myocarditis.

Viral myocarditis, an inflammatory heart disorder caused by viral infections, often leads to poor outcomes due to the lack of effective prevention and treatment strategies. Despite the characteristic feature of cardiomyocyte death in this condition, the underlying mechanisms and potential therapeutic approaches remain poorly understood. Traditional Chinese medicine (TCM), despite its extensive history in treating various diseases, has not yet been approved for the treatment of viral myocarditis.

Overlapping Miller Fisher Syndrome, Bickerstaff Brainstem Encephalitis, and Guillain-Barré Syndrome without glycolipid antibodies-a case report and literature review.

We present a rare case of a 56-year-old female presenting with overlapping features of Miller Fisher syndrome (MFS), Bickerstaff's brainstem encephalitis (BBE) and Guillain-Barré syndrome (GBS), despite seronegativity for anti-GQ1b, GM1, GD1b, GT1a, and GT1b IgG antibodies. The patient developed sequential neurological deficits (ophthalmoplegia, ataxia, tetraparesis, and altered consciousness) following an upper respiratory infection. Electrophysiological studies confirmed acute motor-sensory axonal neuropathy, while cerebrospinal fluid analysis showed albuminocytological dissociation.

MS4A6A/Ms4a6d deficiency disrupts neuroprotective microglia functions and promotes inflammation in Alzheimer's disease model.

Background: Alzheimer's disease (AD) is the most common type of dementia. Genetic polymorphisms are associated with altered risks of AD onset, pointing to biological processes and potential targets for interventions. Consistent with the important roles of microglia in AD development, genetic mutations of several genes expressed on microglia have been identified as risks for AD.

Targeted Submetabolome Profiling of Bufadienolides in Venenum Bufonis Using Scheduled Diagnostic Product Ion-Pair Filtering and Substructure Recognition-Based Strategy.

Introduction: Venenum bufonis (VB), a traditional animal-derived medicine, exhibits significant antitumor, immunomodulatory, and analgesic activities that are primarily attributed to bufadienolides.

Objective: This study presented a novel targeted submetabolomics strategy for comprehensive profiling of bufadienolides in VB.

Material And Methods: This targeted submetabolome mainly integrated scheduled diagnostic product ion (DPI) pair-based filtering and substructure recognition-based structural assembly based on the LC-MS platform.

Association Between Phenoage With Cognitive Function and Cerebrospinal Fluid Biomarkers of Alzheimer's Disease Pathology: The CABLE Study.

Biological age, reflecting an individual's physiological state and function, may vary more or less than chronological age. This study aimed to probe the association between biological age (phenoage) with cognitive function and pathological biomarkers of Alzheimer's disease (AD) in cerebrospinal fluid (CSF). All population were recruited from the Chinese Alzheimer's Biomarker and Lifestyle (CABLE) study.

Early radiological adjacent segment changes following L4/5 fusion: a retrospective comparative study of oblique lateral interbody fusion and minimally invasive transforaminal lumbar interbody fusion.

Background: Adjacent segment disease (ASD) has become one of the most common complications after lumbar interbody fusion. To date, there have been few reports on the radiological effects between oblique lateral interbody fusion (OLIF) and minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF) in lumbar degenerative diseases adjacent to the superior and inferior segments.

Method: The data of patients treated with OLIF or MIS-TLIF due to L4/5 degenerative lumbar diseases from October 2018 to March 2022 were retrospectively analyzed.

Fungal laccase-guided copper nanoflower assembly enables complete conversion of HMF to FDCA.

The growing demand for sustainable biomanufacturing has driven significant interest in 2,5-furandicarboxylic acid (FDCA), a bio-based platform chemical for producing renewable polymers. The eco-friendly oxidoreductase laccase exhibits promising FDCA biosynthesis capacity yet is hampered by pH-dependent activity decay and susceptibility to proteolytic degradation. Herein, we developed a bio-enabled synthesis approach to fabricate three-dimensional laccase-integrated copper hybrid nanoflowers (Lac-NFs) through enzyme-metal coordination-driven self-assembly.

Comparative study of glycyrrhizin interaction with plant-derived proteins for encapsulation and delivery of naringenin: Role of prolamins.

This study evaluated dipotassium glycyrrhizinate (DG) modification on four distinct plant-based alternative proteins: legume-derived faba bean protein (mainly globulins), oilseed pumpkin protein (mainly globulins), pseudocereal buckwheat protein (mainly globulins and few prolamins), and cereal zein (exclusive prolamins), to enhance delivery efficiency of naringenin. Through pH-shifting preparation, DG significantly enhanced protein solubility and naringenin encapsulation efficiency (∼78.0-98.

Efficacy and safety of toripalimab in the treatment of nasopharyngeal carcinoma: a meta-analysis of single-arm trials.

Background: Nasopharyngeal carcinoma (NPC) is common in East Asia, and treatment options are limited. Toripalimab, a PD-1 inhibitor, has potential in NPC therapy.

Objective: This meta-analysis aimed to evaluate the efficacy and safety of toripalimab in the treatment of NPC.

Phthiriasis palpebrarum: A systematic review and illustrative case presentation.

To investigate the clinical features, diagnostic methods, and treatment modalities of Phthiriasis palpebrarum (PP). We conducted a systematic review of case reports and case series of PP by searching MEDLINE, Embase, and ISI Web of Knowledge databases. Demographic and clinical features were extracted and reviewed.

Effect of Hypertension on Long-term Adverse Clinical Outcomes and Liver Fibrosis Progression in MASLD.

Background & Aims: Hypertension is common in metabolic dysfunction-associated steatotic liver disease (MASLD), but its impact on long-term clinical outcomes and disease progression remains unclear. This study investigated the association of hypertension and risk of adverse clinical outcomes and progression of liver stiffness/fibrosis in MASLD.

Methods: Three multicenter prospective cohorts were analyzed: the UK BioBank (UKBB) cohort to assess the risk of adverse clinical outcomes, the VCTE-Prognosis cohort to assess liver stiffness/fibrosis progression, and the Paired Liver Biopsy cohort to assess histologic liver fibrosis progression.

Spinal cord ischemia reperfusion injury induces cuproptosis in neurons.

Background: Spinal cord ischemia reperfusion injury (SCIRI) is a serious disease that can result in irreversible neuronal damage, leading to the loss of sensory and motor function. Cuproptosis, a novel form of regulated cell death, has been studied in various diseases. However, the role and mechanism of cuproptosis in SCIRI remain to be elucidated.

Recent advances in tumor immunotherapy based on NK cells.

Immunotherapy has emerged as the established fourth pillar of cancer treatment following surgery, radiotherapy, and chemotherapy, representing a cutting-edge research domain in translational medicine and clinical oncology. Natural killer (NK) cells, a type of innate cytotoxic lymphocyte, possess unique antitumor properties that are independent of major histocompatibility complex (MHC) restrictions, making them promising candidates for "off-the-shelf" therapeutic products. NK cells can eliminate tumor cells through various mechanisms.

Minimally invasive approaches to obesity: Evaluating the efficacy and safety of endoscopic gastroplasty.

Obesity is a major global health issue, contributing to numerous comorbidities such as type 2 diabetes mellitus, hypertension, and cardiovascular diseases. While bariatric surgery remains the gold standard for treating severe obesity, its invasive nature has led to the development of minimally invasive alternatives. Endoscopic sleeve gastroplasty (ESG) is a promising, minimally invasive procedure for weight loss, offering significant weight reduction without the need for surgical incisions.

Correlation of β2-microglobulin with postoperative delirium and 3-year mortality undergoing knee or hip replacement surgery: a prospective cohort study.

Introduction: Postoperative delirium (POD) is a commonly occurring condition in the postoperative period. Therefore, the study intends to investigate the relationship between BM and POD and the effect of BM levels on three-year postoperative mortality in patients with POD.

Methods: Postoperatively, the Confusion Assessment Method (CAM) and the Monumental Delirium Assessment Scale (MDAS) were used to assess the incidence and severity of POD.

The IRE1α/XBP1-mediated upregulation of LMTK2 attenuates endoplasmic reticulum stress by enhancing autophagic activity.

Lemur tyrosine kinase 2 (LMTK2), a transmembrane protein, is not well characterized regarding its biological functions and regulatory mechanisms. Herein, we demonstrate that LMTK2 functions as an ER stress-induced protein that plays a crucial role in regulating ER stress and safeguarding cells through autophagy pathway. Our study reveals that ER stressors thapsigargin (Tg) and tunicamycin (Tm) upregulate LMTK2 expression via IRE1α-XBP1s signaling in colon cancer cells.

Modified Continuous Figure-of-Eight Suturing Method Between Subcutaneous and Subconjunctival Tissue for Buried-Suture Double-Eyelid Blepharoplasty.

The double-eyelid crease formation is primarily influenced by the attachment of the levator aponeurosis to the skin. The buried-suture method of double-eyelid blepharoplasty has gained popularity due to advantages like minimal scarring, faster recovery, and lack of suture removal. However, traditional techniques often rely on tactile feedback, leading to imprecise fixation and postoperative complications such as asymmetry and suture irritation.

Unveiling Gastrointestinal Involvement in Hypopituitarism: Clinical Features and Treatment Approaches.

Background: Hypopituitarism, characterized by the deficiency of one or more pituitary hormones, can lead to diverse gastrointestinal (GI) manifestations that are often under-recognized, particularly in elderly patients. These symptoms, ranging from nausea and constipation to severe complications like malabsorption and hepatic dysfunction, can significantly impact patient outcomes if not addressed promptly.

Objective: This review aims to elucidate the GI implications of hypopituitarism, focusing on the clinical features, underlying mechanisms and therapeutic approaches, with an emphasis on hormone replacement therapy (HRT) and its potential benefits and risks.