Sensitization of cancer cells to DNA-damaging agents by expression of the REV1 C-terminal domain: Implications for chemotherapy.

The mutagenic translesion synthesis (TLS) pathway, which is critically dependent on REV1's ability to recruit inserter TLS polymerases and the POLζ extender polymerase, enables cancer cells to bypass DNA lesions while introducing mutations that likely contribute to the development of chemotherapy resistance and secondary malignancies. Targeting this pathway represents a promising therapeutic strategy. Here, we demonstrate that the expression of the C-terminal domain (CTD) of human REV1, a ca.

Pseudoduganella rhizocola sp. nov., Isolated from Rhizospheric Soil.

A novel bacterial strain, SM-13 was isolated from the rhizospheric soil of Epipremnum aureum (Jade Pothos) sampled in Suwon, Republic of Korea. The isolate was Gram-stain-negative, aerobic, motile, rod-shaped, cream-coloured, oxidase- and catalase-positive. Strain SM-13 grew at the range of 15-37 °C (optimum, 25 °C), at pH 6.

Primary production by the purple nonsulfur bacterium in an acidic, moderately sulfidic warm spring.

Populations of the acidophilic purple nonsulfur bacterium were identified in two geographically distinct thermal areas in Yellowstone National Park (Wyoming, USA), as confirmed by 16S rRNA gene sequencing and detection of characteristic methoxylated ketocarotenoids. Microcosm-based carbon uptake assays where oxygenic photosynthesis was excluded via addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea yielded a light-driven dissolved inorganic carbon (DIC) assimilation rate (7 ± 2 mg C g C h) comparable to those of highly productive algal mats in acidic hot springs, suggesting that may be performing photoautotrophy at the time of the assay. Rates of acetate assimilation were more than two orders of magnitude lower than DIC assimilation and did not differ between light and dark treatments, indicating photoheterotrophic use of acetate was not occurring, though photoheterotrophic assimilation of other organic compounds cannot be excluded.

Environmental Stresses Constrain Soil Microbial Community Functions by Regulating Deterministic Assembly and Niche Width.

Increasing evidence indicates that the loss of soil microbial α-diversity triggered by environmental stress negatively impacts microbial functions; however, the effects of microbial α-diversity on community functions under environmental stress are poorly understood. Here, we investigated the changes in bacterial and fungal α- diversity along gradients of five natural stressors (temperature, precipitation, plant diversity, soil organic C and pH) across 45 grasslands in China and evaluated their connection with microbial functional traits. By quantifying the five environmental stresses into an integrated stress index, we found that the bacterial and fungal α-diversity declined under high environmental stress across three soil layers (0-20 cm, 20-40 cm and 40-60 cm).

Anemia and iron deficiency in post-kidney transplantation: an unsolved challenge.

Anemia and iron deficiency (ID) are common and significant complications in kidney transplant recipients (KTRs) that can affect their health-related quality of life (HRQoL) and outcomes. Current anemia guidelines equate the post-transplant situation with the anemia associated with chronic kidney disease (CKD) in non-transplanted persons, not acknowledging relevant differences ranging from pathophysiology to clinical manifestation. Nephrologists caring for these patients tend to pay less attention to post-transplant anemia (PTA) and ID than in non-transplanted persons with CKD.

Dual-synergistic molybdenum disulfide and molybdenum carbide cocatalyst integrated with graphitic carbon nitride for efficient photocatalytic hydrogen production.

Heterojunctions have garnered significant attention in the field of photocatalysis due to their exceptional ability to facilitate the separation of photogenerated charge carriers and their high efficiency in hydrogen reaction. However, their overall photocatalytic performance is often constrained by electron transport rates and suboptimal hydrogen adsorption/desorption kinetics. To address these challenges, this study develops a g-CN/MoS@MoC dual-effect synergistic solid-state Z-type heterojunction, synthesized through the in-situ sulfurization of MoC combined with ultrasonic self-assembly technique.

Synergistic interface and oxygen/nitrogen vacancy engineering in g-CN/CuO under high pressure for efficient CO photoreduction.

This study explores highly active nitride-based g-CN/CuO photocatalysts for CO photoconversion by synthesizing them through high-pressure torsion (HPT) straining. Data indicate that increasing the applied strain under high pressure promotes vacancy formation and improves the electronic interaction at the g-CN/CuO interphases, enabling superior charge separation and extended light absorption. The generation of dual vacancies of oxygen and nitrogen is verified by electron paramagnetic resonance and Fourier transform infrared spectroscopic methods, and the generation of a type-II heterojunction is confirmed by band structure analysis.

Z-scheme Heterojunction on TS-1 Zeolite Boosting Ultrafast Visible-Light-Driven Degradation of Cr(VI) and Tetracycline.

Photocatalysis has emerged as a promising strategy to address water pollution caused by heavy metals and antibiotics. Zeolites exhibit significant potential in petrochemical catalysis; however, the development of zeolite-based photocatalysts remains a critical challenge for researchers. Herein, a novel Z-scheme heterojunction was designed and fabricated on the titanium-silicon zeolite TS-1 by modifying g-CN via a simple calcination process.

Genomic and morphological characterization of a novel iridovirus, bivalve iridovirus 1 (BiIV1), infecting the common cockle ().

High rates of mortality of the common cockle, , have occurred in the Wash Estuary, UK, since 2008. A previous study linked the mortalities to a novel genotype of , with a strong correlation between cockle moribundity and the presence of . Here, we characterize a novel iridovirus, identified by chance during metagenomic sequencing of a gradient purification of cells, with the presence also correlated to cockle moribundity.

sp. nov., a novel halotolerant, flexirubin-type pigment-producing bacterium of the family .

A Gram-staining-negative, non-motile, aerobic, rod-shaped bacterium, designated 14752, was isolated from a saline lake in Xinjiang Uygur Autonomous Region, China. The strain was subjected to a taxonomic study using a polyphasic approach. Strain 14752 was able to grow at 4-40 ℃ (optimum 28 ℃), pH 6.

Statins in Acute Ischemic Stroke: Mechanisms, Resistance, and Precision Strategies for Neurovascular and Cognitive Protection.

Acute ischemic stroke (AIS) remains a leading cause of mortality and long-term disability globally, with survivors at high risk of recurrent stroke, cardiovascular events, and post-stroke dementia. Statins, while widely used for their lipid-lowering effects, also possess pleiotropic properties, including anti-inflammatory, endothelial-stabilizing, and neuroprotective actions, which may offer added benefit in AIS management. This article synthesizes emerging evidence on statins' dual mechanisms of action and evaluates their role in reducing recurrence, improving survival, and mitigating cognitive decline.

g-CN/BiO hetero-nanosheets as a superior electrocatalyst for nitrate reduction to ammonia.

The faradaic efficiency of the electro-synthesis of ammonia using the nitrate reduction reaction (NORR) relies on an electrocatalyst to hydrogenate NO and simultaneously suppress the hydrogen evolution reaction (HER). Due to the formation of a heterostructure, the faradaic efficiency of g-CN/BiO reaches 91.12% at -0.

Niabella insulamsoli sp. nov., Isolated From Soil and Showing Potential Cosmetic Functions with Flexirubin Extract.

A yellow-pigmented, non-motile, rod-shaped, and Gram-stain-negative bacterium was isolated from the soil of Yeongheung Island, Korea. The novel isolate, strain N803, was strictly aerobic, grew optimally at 30-35 °C, at pH 6.5, and in the presence of 0-2% NaCl.

Partial D variant with demonstrated risk of D alloimmunization in the Australian Indigenous population.

Background And Objectives: Two prior publications have identified a novel RHD variant in the Australian population with the pattern of single nucleotide variation (SNV) c.186G>T, c.410C>T, c.

sp. nov., isolated from the upper respiratory tract of duck.

Five bacterial strains, designated as RCAD1438, RCAD1439, RCAD1670, RCAD1671 and RCAD1672, were isolated from the upper respiratory tract of ducks in Anhui, Shaanxi and Sichuan, China. All strains are Gram-stain-negative, rod-shaped, non-motile, non-spore-forming, aerobic and capsulated. They grow optimally at 37 °C and pH 7.

Genomic insights, determination of quorum quenching potential of a beta-lactamase enzyme from Chromohalobacter sp. strain D23 against Aeromonas hydrophila and molecular docking study.

Biofilm formation and other virulence phenotypes under quorum sensing regulation play a vital role in the pathogenicity of Aeromonas hydrophila, triggering the emergence of multi-drug resistance (MDR) which increases fish mortality, environmental issues, and economic loss in aquaculture, necessitating the discovery of novel drugs to bypass standard antibiotics. Here, quorum quenching (QQ) may be a sustainable anti-virulent approach. β-Lactamase enzyme obtained from Chromohalobacter sp.

TgAP2X-7 is a novel cell cycle-regulated transcription factor that plays an essential role in tachyzoite propagation.

Apicomplexan AP2 (ApiAP2) family proteins are a family of transcription factors that are known to regulate gene expression in apicomplexan pathogens, including . In this study, we focused on TgAP2X-7, a member of the APiAP2 family that is predicted to be essential for fitness. Endogenous tagging of TgAP2X-7 followed by immunofluorescence analysis revealed that it's a cell cycle-regulated nuclear protein with peak expression in the G1 phase.

Slt2 positively regulates Myb-mediated cellulose utilization in .

Unlabelled: Fungal degradation of cellulose facilitates the sustainable harnessing of biosphere energy and carbon cycling. is one of the basidiomycetes with the largest number of hydrolytic enzymes in its genome. The mycelium of degrades cellulose through the production of substantial amounts of cellulase, enabling the absorption of carbon sources and nutrients essential for fruiting body development.

Comparison of the two intestinal anastomosis methods in pediatric patients.

Background: For pediatric patients, there is still controversy regarding the anastomotic technique used for gastrointestinal construction. The study was to evaluate the continuous single-layer (CSL) intestinal anastomosis method compared with the two-layered interrupted anastomosis.

Methods: We retrospectively reviewed the medical records of the eligible patients following CSL anastomosis ( = 252) and interrupted double-layer (IDL) anastomosis ( = 196).

Heterostructured assembly of ternary SnS/MoS/g-CN nanocomposites for high-performance hybrid supercapacitors, photodegradation of organic dyes and Cr(VI) reduction.

Designing heterostructure-based nanocomposites has gained considerable interest in solving energy scarcity and environmental contamination issues. Herein, a heterojunction assembly of ternary SnS/MoS/g-CN nanocomposites with varying Sn and Mo weight ratios was synthesized through a single-step hydrothermal method. At an optimized ratio of tin to molybdenum (1 : 2), denoted as SM-3, promising electrochemical and photocatalytic performances were observed compared to bare SnS/g-CN and MoS/g-CN.

Macrophage Migration Inhibitory Factor: Its Multifaceted Role in Inflammation and Immune Regulation Across Organ Systems.

Macrophage Migration Inhibitory Factor (MIF) is a pleiotropic cytokine that acts as a central regulator of inflammation and immune responses across diverse organ systems. Functioning upstream in immune activation cascades, MIF influences macrophage polarization, T and B cell differentiation, and cytokine expression through CD74, CXCR2/4/7, and downstream signaling via NF-κB, ERK1/2, and PI3K/AKT pathways. This review provides a comprehensive analysis of MIF's mechanistic functions under both physiological and pathological conditions, highlighting its dual role as a protective mediator during acute stress and as a pro-inflammatory amplifier in chronic disease.

One-pot synthesis of a polydopamine-glued g-CN/CoFeWO membrane for synergistic photocatalytic water purification.

A polydopamine-glued g-CN/CoFeWO membrane, prepared one-pot synthesis, achieves complete sulfamethoxazole degradation through synergistic photocatalysis and PMS activation. It exhibits robust stability over 10 hours of continuous operation, maintaining high efficiency (97%) even in real municipal wastewater effluent, offering a novel and promising water purification strategy.

Genomic analysis and preclinical evaluation of two hydrogel-formulated novel virulent phages isolated against a carbapenem-resistant Acinetobacter baumannii.

Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) has recently become a serious cause for global concern because of non-susceptibility to multiple antimicrobial classes, its prevalence in nosocomial infections, and the lack of effective treatments against such a pathogen.

Methods: This study isolated two lytic phages from hospital sewage, purified, propagated, characterized morphologically by transmission electron microscopy, and genomically by Oxford Nanopore Sequencing. The phage lysates were then formulated individually as carboxymethylcellulose (CMC) 5 % w/v hydrogels.

Development of an effective DNA vaccine against ayu atypical cellular gill disease using draft genome information of the causative agent, Plecoglossus altivelis poxvirus.

Atypical cellular gill disease (ACGD) in ayu (Plecoglossus altivelis) caused by P. altivelis poxvirus (PaPV) infection has led to significant economic losses in Japanese aquaculture. The propagation of PaPV has not yet been successfully achieved in cultured cells.