Terbium (III) and adenosine monophosphate coordinated hydrogel for fluoroquinolones sensing by combining machine learning.

Nondestructive identification of fluoroquinolones distribution on food is an approach to simplify detection steps and improve detection efficiency. In this study, a fluorescent sensor based on terbium (III) and adenosine monophosphate coordinated hydrogel (TbCHy) has been synthesized, which is assisted by machine learning for intelligent and nondestructive detection of fluoroquinolones (FQs) in meat. Norfloxacin (NOR) was used as the model target, the TbCHy-based sensor performed a good linearity when NOR concentration ranged from 0.

Comparative disinfection efficacy in aquaculture: novel methylene bis(thiocyanate) vs. conventional trichloroisocyanuric acid against .

is a major pathogen responsible for significant economic losses in global aquaculture. Inhibitory and bactericidal activities of seven disinfectants were tested against 10 aquatic pathogens, including , , , , , , , and . Minimal effective concentrations were determined via quantitative suspension tests, while a scale-trauma zebrafish model assessed protection.

Ultrasoft, anti-dehydrated, and highly stretchable carboxymethylcellulose-based organohydrogel strain sensors for non-invasive real-time plant growth monitoring.

Plant strain sensors, which enable real-time monitoring of plant growth, play a crucial role in improving both the quality and yield of various plants. However, current plant strain sensors often exhibit significant mechanical mismatches and poor interfacial compatibility with plant tissues, leading to inaccurate and unstable in situ monitoring. Herein, we present a novel ultrasoft, anti-dehydrated, and highly stretchable organohydrogel (UASOH) strain sensor composed of polyacrylamide (PAM) and carboxymethylcellulose (CMC) via a glycerol-induced hydrogen bonding enhancement strategy.

Dual cyp17a1/a2 knockout unveils paralog-specific steroid pathways in fish reproduction.

Cyp17a is a key enzyme in steroidogenesis and reproduction in vertebrates. In teleosts, two paralogous genes, cyp17a1 and cyp17a2, have been identified, and while the potential impact of mutations in these genes have been studied, a comprehensive comparative analysis of their regulatory mechanisms in teleost reproduction remains lacking. In this study, we established a double mutant (dko) of cyp17a1 and cyp17a2 and conducted a transcriptome analysis of their gonads to systematically investigate the differences in steroidogenesis and gene expression between the two genes.

Coupled nitrogen and phosphorus cycles mediated by coordinated variations of functional microbes in industrial recirculating aquaculture system.

Industrial Recirculating Aquaculture Systems (IRAS) represent a sustainable and efficient approach to aquaculture, offering significant benefits in water conservation and environmental management. A comprehensive understanding of nitrogen (N) and phosphorus (P) cycling is essential for optimizing system design and operational strategies, enabling the maintenance of a balanced ecosystem within IRAS. Here, water microbial communities in the shrimp aquaculture pond (AP) and nitrification tank (NT) of the IRAS were investigated using a metagenomics-based approach to explore the mechanisms of N and P coupling cycles.

Thermal-Sensitive Artificial Ionic Skin with Environmental Stability and Self-Healing Property.

Wearable temperature-sensitive electronic skin enables robots to rapidly detect environmental changes and respond intelligently, thereby reducing temperature-related mechanical failures. Additionally, this temperature-sensitive skin can measure and record the temperature of external objects, broadening its potential applications in the medical field. In this study, we designed a thermally sensitive artificial ionic skin using ionic liquids (ILs) as solvents and carbon nanotubes (CNTs) as thermally conductive fillers.

Transcriptome-derived evidence reveals the regulatory network in the skeletal muscle of the fast-growth mstnb male tilapia.

Myostatin (Mstn) negatively regulates muscle growth and Mstn deficiency induced "double-skeletal muscle" development in vertebrates, including tilapias. In this study, we performed a transcriptomic analysis of skeletal muscle from both wild-type and mstnb males to investigate the molecular mechanisms underlying skeletal muscle hypertrophy in mstnb mutants. We identified 4697 differentially expressed genes (DEGs), 113 differentially expressed long non-coding RNAs (DE lncRNAs), 211 differentially expressed circular RNAs (DE circRNAs), and 98 differentially expressed microRNAs (DE miRNAs).

Investigating the molecular mechanisms of oocyte maturation and ovulation in Nile tilapia: A focus on the steroidogenic enzyme Cyp17a2.

Previous research has highlighted the significant role of progestins and glucocorticoids in fish oocyte maturation and ovulation. To clarify the molecular mechanisms underlying these processes, comprehensive investigations were conducted using a mutant Nile tilapia ( ) model. Analysis revealed pronounced Cyp17a2 expression in ovarian somatic cells of the tilapia.

Hypernetwork modeling and topology of high-order interactions for complex systems.

Interactions among the underlying agents of a complex system are not only limited to dyads but can also occur in larger groups. Currently, no generic model has been developed to capture high-order interactions (HOI), which, along with pairwise interactions, portray a detailed landscape of complex systems. Here, we integrate evolutionary game theory and behavioral ecology into a unified statistical mechanics framework, allowing all agents (modeled as nodes) and their bidirectional, signed, and weighted interactions at various orders (modeled as links or hyperlinks) to be coded into hypernetworks.

Hypogonadotropic hypogonadism in male tilapia lacking a functional rln3b gene.

Relaxin 3 is a neuropeptide that plays a crucial role in reproductive functions of mammals. Previous studies have confirmed that rln3a plays an important role in the male reproduction of tilapia. To further understand the significance of its paralogous gene rln3b in male fertility, we generated a homozygous mutant line of rln3b in Nile tilapia.

Star1 gene mutation reveals the essentiality of 11-ketotestosterone and glucocorticoids for male fertility in Nile Tilapia (Oreochromis niloticus).

Steroidogenic acute regulatory protein (Star) plays an essential role in the biosynthesis of corticosteroids and sex steroids by mediating the transport of cholesterol from the outer to the inner membrane of mitochondria. Two duplicated Star genes, namely star1 and star2, have been identified in non-mammalian vertebrates. To investigate the roles of star genes in fish steriodogenesis, we generated two mutation lines of star1 and star1/star2 in Nile tilapia (Oreochromis niloticus).

Genome-wide analysis of two different regions of brain reveals the molecular changes of fertility related genes in rln3a mutants in male Nile tilapia (Oreochromis niloticus).

Relaxin3 (rln3) has been associated with various emotional and cognitive processes, including stress, anxiety, learning, memory, motivational behavior, and circadian rhythm. Notably, previous report revealed that Rln3a played an indispensable role in testicular development and male fertility in Nile tilapia (Oreochromis niloticus). However, the underlying molecular mechanisms remain largely unknown.

Contribution of to Docosahexaenoic Acid (DHA) Synthesis at the Transcriptional Regulation Level in Common Carp, .

Docosahexaenoic acid (DHA) is an essential nutrient for humans and plays a critical role in human development and health. Freshwater fish, such as the common carp (), have a certain degree of DHA biosynthesis ability and could be a supplemental source of human DHA needs. The elongase of very-long-chain fatty acid 5 (Elovl5) is an important enzyme affecting polyunsaturated fatty acid (PUFA) biosynthesis.

A pleiotropic-epistatic entangelement model of drug response.

Because drug response is multifactorial, graph models are uniquely powerful for comprehending its genetic architecture. We deconstruct drug response into many different and interdependent sub-traits, with each sub-trait controlled by multiple genes that act and interact in a complicated manner. The outcome of drug response is the consequence of multileveled intertwined interactions between pleiotropic effects and epistatic effects.

Cyp17a2 is involved in testicular development and fertility in male Nile tilapia, .

Background: Steroid hormones play an essential role in many reproductive processes of vertebrates. Previous studies revealed that teleost-specific Cyp17a2 (cytochrome P450 family 17 subfamily a 2) might be required for the production of cortisol in the head-kidney and 17α,20β-dihydroxy-4-pregnen-3-one (DHP) in ovary during oocyte maturation. However, the role of Cyp17a2 in male reproduction remains to be largely unknown.

Dynamic Elimination of Enrofloxacin Under Varying Temperature and pH in Aquaculture Water: An Orthogonal Study.

Orthogonal experiments were used to simulate the enrofloxacin (ENR) elimination dynamic in deeper water of aquaculture. Two factors at values common in fishery water (temperature of 20°C, 25°C, and 30°C; pH of 5, 7, and 9) were studied. The degradation of ENR in the nine treatment groups ranged from 44.

Effects of fenvalerate concentrations and its chiral isomers on bacterial community structure in the sediment environment of aquaculture ponds.

To investigate the effect of chiral pesticide fenvalerate (FV) on the micro-ecological environment of aquaculture pond sediment, we used an indoor static experiment to observe the effects of FV added at different concentrations with different chiral isomers on the changes in the sediment bacterial community. The 16S rDNA high-throughput sequencing technique was used to conduct sequencing and analysis of the bacterial community structure as well as changes in aquaculture pond sediments after 4 weeks of cultivation. The results showed that the microbial alpha diversity indices (Sobs and Shannon indices) of the treated groups were significantly lower than those of the control group after 4 weeks (P < 0.

Correction to: The effect of teprenone on the intestinal morphology and microbial community of Chinese sea bass (Lateolabrax maculatus) under intermittent hypoxic stress.

In the end of the Discussion section, following information should be included.

The effect of teprenone on the intestinal morphology and microbial community of Chinese sea bass (Lateolabrax maculatus) under intermittent hypoxic stress.

Hypoxia stress may affect the fish intestine and thereby threaten the growth and survival of the fish. Teprenone is a clinically effective agent in protecting gastrointestinal mucosa. This study aims to assess the effect of teprenone in the intestine of Chinese sea bass Lateolabrax maculatus under intermittent hypoxic stress.

Transcriptome alterations in female Daphnia (Daphnia magna) exposed to 17β-estradiol.

The molecular mechanism of evaluating 17β-estradiol (E)-induced toxicity in female Daphnia magna has not been determined. In this study, the transcriptome of D. magna was analyzed after exposure to three different concentrations (0, 10, and 100 ng L) of E at 3, 6, and 12 h.

Transcriptomic analysis of juvenile Chinese sea bass (Lateolabrax maculatus) anesthetized by MS-222 (tricaine methanesulfonate) and eugenol.

MS-222 (tricaine methanesulfonate) and eugenol are the two frequently used fish anesthetics. This study intends to analyze the regulation of these anesthetics in Chinese sea bass, Lateolabrax maculatus, through transcriptomic analysis. L.