Association of METS-IR with Pregnancy Outcomes in Euthyroid Women: A Retrospective Cohort Study.

The non-insulin-based metabolic score for insulin resistance (METS-IR) is a recently developed index aimed at being a practical and efficient alternative biomarker of insulin resistance (IR). This study aimed to investigate the association between METS-IR in euthyroid women in the first trimester of pregnancy and pregnancy outcomes. A total of 1810 participants who gave birth at Fujian Maternity and Child Health Hospital from November 2018 to November 2019 were included in this study.

Efficacy and safety of haploidentical hematopoietic stem cell transplantation combined with umbilical cord blood infusion in hematologic malignancies: a prospective phase II clinical trial.

Background: Haploidentical hematopoietic stem cell transplantation (haplo-HSCT) offers curative potential for hematologic malignancies but is often limited by high incidences of graft-versus-host disease (GVHD), delayed engraftment, and transplant-related mortality-especially when donors are aged ≥40 years. Umbilical cord blood (UCB) infusion may mitigate these risks by promoting immune tolerance and hematopoietic recovery. However, the efficacy of this strategy in the context of older donors remains insufficiently studied.

Electric Field-Induced Droplet Detachment and Transport on Biomimetic Superhydrophobic Surfaces with Beetle Elytra-Seta Composite Structures.

The regulation of droplet dynamics based on external electric fields and bioinspired functional surfaces has widespread applications in various fields. However, research on the coupling of these two factors to enhance oil-water separation efficiency is urgently needed. In this study, laser-induced and solvent treatment techniques were coupled to assemble a micronano setal and bioinspired beetle elytra textured substrate with the lotus effect, A "top conductive, bottom insulating" Desert beetle elytra micronano tuft composite texture (DBE) biomimetic superhydrophobic surface was fabricated.

Facilitators and barriers to the implementation of school mental health collaboration in Sichuan province, China: a descriptive qualitative study.

Purpose: This study aimed to interview stakeholders who participated in school mental health inter-professional collaboration (IPC) and determine the facilitators and obstacles for the development of school mental health inter-professional collaboration in Sichuan province, China.

Method: Semi-structured interviews were conducted with 22 stakeholders, including psychiatrists, nurses, school mental health teachers, community workers, and hospital administrators. Data were analyzed using content analysis guided by the Exploration-Preparation-Implementation-Sustainment (EPIS) framework.

Isolation and identification of a strain of Pseudomonas putida and its effect on histopathology, apoptosis, and gene expression in turbot (Scophthalmus maximus).

Turbot (Scophthalmus maximus) is one of the most widely species that boasts high economic value in the aquaculture industry. In spite of the exponential growth of aquaculture, infectious bacterial diseases have caused great economic losses in turbot industry. This study isolated and identified a strain of Pseudomonas putida, denoted as DY16, and it is a conditionally pathogenic bacterium, therefore we conducted the following research on it.

Negative regulation of the IL-4/13-mediated Th2 immune response by microRNA-126b via targeting IL-4Rα1 in large yellow croaker (Larimichthys crocea).

The closely related cytokines Interleukin-4 (IL-4) and IL-13 regulate the Th2 immune response by interacting with their specific receptor complexes. MicroRNAs (miRNAs) modulate various biological pathways through mechanisms that either repress mRNA translation or promote messenger RNA degradation. The miRNA miR-126b is implicated in fish embryonic development.

Functional characterization of two Caspase-1 transcripts that associated with ASC and NLRC3 in apoptosis and inflammatory response in large yellow croaker Larimichthys crocea.

As a member of the inflammatory caspases, Caspase-1 can increase the host inflammatory response against pathogen invasion and also function dominantly in apoptosis. In this study, we cloned and obtained two transcripts of Caspase-1 in large yellow croaker (Larimichthys crocea), namely Lc-Caspase-1_tv1 and Lc-Caspase-1_tv2. The ORF of Lc-Caspase-1_tv1 is 1,239 bp, whereas Lc-Caspase-1_tv2 is 1,167 bp in length, encoding a protein of 412 and 388 aa, and both of which contains a CARD and a CASc domain.

Crayfish IMD responds rapidly to WSSV infection and the activated IMD-Relish-AMPs pathway inhibits viral replication.

One of the key innate immune pathways in invertebrates is the immune deficiency (IMD) signaling pathway, which effectively combats Gram-negative bacterial infections. In insects, the IMD pathway is involved in the defense against certain viral infections. However, the functional role of the IMD pathway in antiviral immunity remains incompletely characterized in crustaceans.

CgCREM Regulates Haemocyte Proliferation and Inflammatory Factor Expression in the Pacific Oyster Crassostrea gigas.

The cAMP response element modulator (CREM) is a regulatory transcription factor downstream of cAMP signaling, functioning either as a transcriptional activator or repressor in regulating the proliferation and differentiation of immune cells. In the present study, CgCREM with a conserved pKID domain and a BRLZ domain was identified from Pacific oyster Crassostrea gigas. The mRNA transcripts of CgCREM were found to be highly expressed in embryonic stages, especially in the blastula and trochophore.

Blocking T-type calcium channels disrupts spermatogenesis in vivo and adversely affects spermatocytes in vitro by impairing mitochondrial function and autophagic flux.

T-type calcium channels are pivotal in spermatogenesis. To evaluate the molecular mechanisms by which T-type calcium channels regulate spermatogenesis, we constructed animal and cellular models using T-type calcium channel inhibitor flunarizine (FNZ). Intraperitoneal administration of FNZ (30mg/kg) significantly impaired sperm motility, inhibited testicular germ cell proliferation, and disrupted sperm mitochondrial function in male mice.

Protective Effect of Ginsenoside Rg3 on DBP-Induced Spermatogenesis Injury via the Src/PI3K Signaling Pathway.

This study aimed to investigate the protective mechanism of Ginsenoside Rg3 (Rg3) against Di-n-butyl phthalate (DBP) induced spermatogenic damage, focusing on the Src/PI3K/Akt pathway. In vivo experiments demonstrated that Rg3 restored DBP-induced dysregulation of gap junction (GJ) protein connexin 43 (Cx43), improved testicular structure, enhanced sperm parameters (count and motility), and upregulated phosphorylation of Src, PI3K, and Akt (p-Src, p-PI3K, p-Akt) in mice. In vitro studies, using the metabolite of DBP, monobutyl phthalate (MBP), and pathway inhibitors (PP2 for Src and LY294002 for PI3K), further confirmed these effects.

Esculetin Mitigates MRSA Pathogenicity via Innovative Multitarget Inhibition of Staphylococcus aureus Sortase A, Coagulase, and Von Willebrand Factor-binding Protein.

The global proliferation of antibiotic-resistant Staphylococcus aureus, particularly methicillin-resistant Staphylococcus aureus (MRSA), highlights the urgent need for innovative antivirulence strategies. The redundancy and multiplicity of virulence factors produced by S. aureus necessitate interventions capable of concurrently targeting multiple virulence mechanisms.

Shenling Jianpiwei formula ameliorates metabolic associated fatty liver disease through modulation of the gut microbiota and attenuation of LPS-mediated inflammation.

The gut microbiota and its products are recognized as pivotal contributors to the pathogenesis of metabolic-associated fatty liver disease (MAFLD). Shenling Jianpiwei formula (SLJPW), a prescription renowned for its protective effects in intestinal disorders, demonstrates efficacy against MAFLD. However, its underlying mechanisms and chemical composition remain unclear.

Effectiveness and safety of adjunctive transcranial direct current stimulation for adolescents with first-episode major depressive disorder: A randomized, double-blind, sham-controlled trial.

Background: Transcranial direct current stimulation (tDCS), a non-invasive brain stimulation method, can improve depressive symptoms by applying weak electric direct currents to the scalp. This study aimed to evaluate the efficacy and safety of adjunctive tDCS for adolescents with first-episode major depressive disorder (FE-MDD).

Methods: This double-blind, randomized, sham-controlled trial (RCT) was conducted between January 3, 2024, and August 24, 2024.

Association between adverse childhood experiences, adopting a healthy lifestyle in adulthood, and the risks of metabolic disease and multimorbidity: a population-based cohort study.

Background: The detrimental health effects of adverse childhood experiences (ACEs) are well documented, yet their impact on metabolic multimorbidity remains unclear. This study aimed to examine the associations of ACEs with adult metabolic disease and multimorbidity, and to assess whether adopting a healthy lifestyle in adulthood could mitigate these risks.

Methods: We analyzed 70,438 participants without any metabolic disease at enrolment in the UK Biobank.

Associations between loneliness and psychological health symptoms as well as dietary habits in adolescents: Sex differences.

Background: Adolescence is a stage of high prevalence of loneliness. However, research on the associations between loneliness and psychological health as well as dietary habits in adolescents remains limited. Therefore, this study aimed to explore these associations and potential sex differences.

Spousal depressive symptoms and partner's subsequent risk of cardiovascular diseases: A nationwide population-based prospective study in China.

Background: The relationship between spousal depressive symptoms and cardiovascular risk remains understudied despite established links between individual depression and cardiovascular outcomes.

Methods: Using data from 6651 married Chinese adults (≥45 years) in the China Health and Retirement Longitudinal Study (2011-2018), we examined associations between spousal depressive symptoms and incident cardiovascular disease (CVD) using Cox proportional hazards models. Cox models with penalized splines were performed to explore the dose-response relationship.

Exploring the causal relationships between psychiatric disorders and hypothyroidism combining mendelian randomization with network pharmacology.

Background: While associations between psychiatric disorders and hypothyroidism have been observed, the underlying causal relationships remain unclear. Exploring the causal relationships among them can provide new insights into the underlying mechanisms.

Methods: Two-sample Mendelian randomization (MR) was conducted to assess the causal effects of seven psychiatric disorders on hypothyroidism.

Genome-wide identification and functional characterization of rapid alkalinization factor 6 as a key peptide regulator of abiotic stress tolerance in Tartary buckwheat.

Rapid alkalinization factors (RALFs) are cysteine-rich signaling peptides in plants that play critical roles in development, immune regulation, and responses to abiotic stress. Despite their importance, the functional characterization of RALF family members in Tartary buckwheat (Fagopyrum tataricum), a nutrient-rich crop known for its remarkable resilience to multiple stresses, remains largely unexplored. In this study, we conducted a comprehensive genome-wide analysis to identify and characterize the FtRALF gene family in Tartary buckwheat, examining their phylogenetic relationships, gene structures, and duplication events.

High light upregulates the expression of the duplicate glutathione S-transferase genes SbrGSTF10/11 to induce anthocyanin accumulation in Salix brachista.

Glutathione S-transferase (GST, EC 2.5.1.

Developmental Neuroplasticity Enables Recovery from Anesthetic-Induced Synaptic Perturbations in the Immature Brain.

General anesthetics are essential in pediatric medicine, yet concerns persist regarding their potential neurotoxic effects on the developing brain. Whether transient synaptic disruptions caused by anesthesia lead to long-term deficits or are mitigated by endogenous plasticity remains unresolved. Here, we use longitudinal in vivo two-photon imaging in awake mice to investigate the structural and functional consequences of a single, clinically relevant exposure to sevoflurane at postnatal day 20.

Dynamic Interaction of Oligodendrocyte Precursor Cells with Other Cell Types in the Central Nervous System.

Traditionally, oligodendrocyte precursor cells (OPCs) were primarily regarded for their differentiation potential to mature oligodendrocytes that ensheath central nervous system (CNS) axons through myelin formation. Recent breakthroughs in single-cell sequencing and in vivo imaging technologies have revolutionized our understanding, revealing that OPCs engage in extensive dynamic interactions with diverse CNS cell populations during neurodevelopment, tissue homeostasis maintenance, and pathological microenvironment remodeling. Notably, while OPCs exhibit relatively conserved phenotypic signatures, their functional plasticity within heterogeneous microenvironments demonstrates significant spatial specificity and disease-context dependence.