Beyond disenfranchized grief: Survey and interview accounts of animal loss through and beyond COVID-19 in the United Kingdom.

Although there was a rapidly growing body of literature on human-animal relationships during the COVID-19 pandemic, little attention was given to accounts of animal death through and beyond COVID-19. This paper reports on two connected studies undertaken after the end of COVID-19 restrictions in the United Kingdom, here focusing specifically on animal companion loss. From an online survey of 667 participants, 354 responded to an open-ended question about the loss of an animal.

Activation of the Lattice Oxygen Mechanism in NiOOH as an Electrocatalyst for the Oxygen Evolution Reaction: The Role of Anion Intercalation.

The lattice oxygen mechanism (LOM) of the oxygen evolution reaction (OER) offers significant kinetic advantages over the adsorbed oxygen mechanism. Anion intercalation induces the LOM in NiOOH by enhancing the covalency of lattice oxygen through the modulation of the metal-oxygen electronic state. The relationships between doping mechanisms, such as the size and valence state of anions and the kinetics of the OER, have been clarified.

Comparison of 10-2 Visual Field Using Melbourne Rapid Fields Online Perimetry and Humphrey Field Analyzer.

Purpose: Melbourne rapid fields (MRF) online perimetry is web-based software that allows white-on-white threshold perimetry using any computer. This study assesses the perimetric outcomes of MRF10-2 protocol via laptop computer in comparison to Humphrey field analyzer (HFA).

Methods: This prospective and cross-sectional study included 91 eyes from 91 Japanese glaucoma patients.

Altered Corneal T-Cell Motility and Sensory Nerve Features in Older Adults With Human Immunodeficiency Virus Infection.

Purpose: To characterize corneal immune cell morphodynamics and nerve features, and define the in vivo immune landscape in older adults with human immunodeficiency virus (HIV) receiving antiretroviral therapy (ART), relative to healthy age-matched adults.

Methods: In this cross-sectional study, 16 HIV-positive individuals receiving ART and 15 age-matched controls underwent ocular surface examinations and functional in vivo confocal microscopy (Fun-IVCM). Time-lapsed videos were created to analyze corneal immune cells (T cells, dendritic cells [DCs], macrophages).

Evolution and structural diversity of the MotAB stator: insights into the origins of bacterial flagellar motility.

The rotation of the bacterial flagellum is powered by the MotAB stator complex, which converts ion flux into torque. Despite its central role in flagellar function, the evolutionary origin and structural diversity of this system remain poorly understood. Here, we present the first comprehensive phylogenetic and structural characterization of MotAB and its closest non-flagellar homologs.

Pregnancy Outcomes After HIV Diagnosis in Women Living With HIV in Japan, South Korea and Hong Kong Special Administrative Region: A Brief Report.

The study analyzed pregnancy outcomes among 498 women living with HIV in East Asia. We found 15% had pregnancies postdiagnosis, with 57% resulting in live births. Older age at antiretroviral therapy initiation and higher pre-antiretroviral therapy viral loads were negatively associated with pregnancy.

Electrocatalytic Upcycling of PET Waste to Chemical Products and Fuels Coupled With Cathodic Reactions.

Plastic waste continues to be a major environmental challenge, worsened by energy-intensive conventional recycling methods that require highly pure feedstocks. In this review, emerging electrochemical upcycling technologies are critically examined, focusing on the electro-oxidation transformation of polyethylene terephthalate (PET) into valuable chemical products. Key reaction pathways and target products are outlined to clarify the selective electrochemical reforming of PET.

Farm Injury Deaths and Workers' Compensation Claims in Australia and Their Economic Costs.

Objective: To describe the pattern and estimated direct economic burdens associated with unintentional deaths and injuries on Australian farms over the past 11 years (2013-2023).

Design: Descriptive retrospective epidemiological study of National Coronial Information System (NCIS) data for persons fatally injured on a farm and workers' compensation injuries data from the National Data Set.

Setting: Australia.

Coordination-Driven Orthogonal Ligand Pairings through Dual Hydrogen-Bonding/π-π Interaction Complementarity.

Multi-layered and orthogonal recognition is an excellent route to controlled molecular complexity. Here we report a series of heteroleptic complexes where two ligands pair together at a palladium(II) metal centre in complementary fashion and with orthogonality to others pairs. This complementarity is driven in part through hydrogen-bonding acceptor or donor sites proximal to the coordination domain (either DD:AA or AD:DA).

Interpretation of plasma pharmacokinetics and urinary excretion of phenolic metabolites and catabolites derived from (poly)phenols following ingestion of mango by ileostomists and subjects with a full gastrointestinal tract: complications associated with endogenous and ingested phenylalanine and tyrosine.

Consumption of mango has been associated with a number of beneficial effects on health which have been attributed to phenolic catabolites originating from (poly)phenols following ingestion. To investigate the origins of potentially bioactive phenolic catabolites, ileostomists and subjects with a full gastrointestinal tract on a low(poly)phenol diet ingested a mango pulp purée containing 426 μmol of (poly)phenols consisting mainly of gallotannins and cinnamic acids, along with 231 μmol of the aromatic amino acids phenylalanine and tyrosine. Over a 24 h period post-mango intake plasma and urine were collected and analysed by UHPLC-HRMS.

Association between physician specialty and first-attempt intubation success in the emergency department.

Background And Importance: Emergency airway management in the emergency department (ED) is a high-risk procedure associated with patient outcomes. First-attempt success is a widely recognized quality metric, as multiple attempts are associated with an increased risk of peri-intubation complications. In Brazil, where emergency medicine is a recently established specialty, many ED are staffed by physicians without formal emergency medicine training.

Beyond Barriers and Facilitators: A Qualitative Study of the Interests Driving Physician-Led Innovation.

Rationale: Clinical innovation-where physicians develop and use novel interventions that differ significantly from standard practice and that have not been shown to be sufficiently safe or effective for regular use in healthcare systems-has the potential to transform patient care and drive medical advancement. However, it is not without risk. It is important, therefore, that policymakers and healthcare institutions develop strategies to encourage responsible clinical innovation.

Glycerol and glycerol-3-phosphate: multifaceted metabolites in metabolism, cancer and other diseases.

Glycerol and glycerol-3-phosphate are key metabolites at the intersection of carbohydrate, lipid and energy metabolism. Their production and usage are organismal and cell type specific. Glycerol has unique physicochemical properties enabling it to function as an osmolyte, protein structure stabilizer, antimicrobial and antifreeze agent, important to preservation of many biological functions.

Characterization of Fluorescent Reporters for Flow Cytometry-Based Single-Cell Studies in .

Fluorescent proteins (FPs) are commonly used as reporters to examine intracellular genetic, molecular, and biochemical status. Flow cytometry is a powerful technique for accurate quantification of single-cell fluorescent levels. Here, we characterize green, red, and blue FPs for use in yeast .

Surface Topography and Ultrastructure of the Spectacular Cells in the Eyes of Land and Sea Snakes (Squamata, Reptilia): Functional Adaptations of Micro-Ornamentation.

Although the surface micro-ornamentation of the scales within the skin of snakes has been the subject of many previous studies, there has been little work done on the spectacle, a protective (keratinised) goggle separated from the underlying cornea by a sub-spectacular space. The surface ultrastructure of the "Oberhäutchen" of the spectacle is examined in nine species of snakes (five aquatic and four terrestrial) using light and electron microscopy, micro-computed tomography and gel-based profilometry. Significant topographic differences in cell size (increases of between 5.

The effect of daily sunscreen application on vitamin D: findings from the open-label, randomised, controlled Sun-D Trial.

Background: Sunscreen reduces vitamin D production in experimental studies. It is uncertain whether this translates to 'real-world' settings.

Objectives: We aimed to dtermine if routinely applying high-SPF sunscreen for one year reduces serum 25-hydroxyvitamin D [25(OH)D] concentration.

Efficacy of Tenecteplase in Large Vessel Occlusion Stroke Within 24 Hours of Symptom Onset: The ETERNAL-LVO Randomized Controlled Trial.

Background: To assess the efficacy and safety of tenecteplase in patients presenting within 24 hours of symptom onset with a large vessel occlusion and target mismatch on perfusion computed tomography.

Methods: ETERNAL-LVO was a prospective, randomized, open-label, blinded end point, phase 3, superiority trial where adult participants with a large vessel occlusion, presenting within 24 hours of onset with salvageable tissue on computed tomography perfusion, were randomized to tenecteplase 0.25 mg/kg or standard care across 11 primary and comprehensive stroke centers in Australia.

Drug-Eluting Resorbable Scaffold Versus Balloon Angioplasty for Below-the-Knee Peripheral Artery Disease: 2-Year Results From the LIFE-BTK Trial.

Background: Limited treatment options exist for infrapopliteal disease in patients with chronic limb-threatening ischemia (CLTI), a condition associated with a high risk of limb loss. Interventional management of diseased infrapopliteal vessels with percutaneous transluminal angioplasty (PTA) is associated with high rates of restenosis and reintervention. In the LIFE-BTK trial, the drug-eluting resorbable scaffold (DRS) demonstrated superior 12-month efficacy compared with PTA in a selected CLTI population with predominantly noncomplex, mildly to moderately calcified lesions.

Temporal trends in gender-affirming hormone therapy initiation: evidence from whole-of-population Australian administrative data.

Using longitudinal national prescribing data, we estimated the number of transgender and gender-diverse individuals initiating gender-affirming hormone therapy (GAHT) from 2013 to 2024 in Australia. Between 2013 and 2024, 11 883 individuals initiated testosterone-based GAHT and 20 358 initiated oestrogen-based GAHT. Initiation rose from 1118 in 2013 to 5135 in 2024, with a growing share accessing testosterone-based GAHT over time.

The Burden of Motor Neuron Diseases in the United States, 1990-2021: A Systematic Analysis of the Global Burden of Disease Study 2021.

Introduction/aims: There is a lack of up-to-date information on the burden of motor neuron diseases (MNDs) in the United States (US). This study aimed to estimate trends in the prevalence, incidence, mortality, and disability-adjusted life years (DALYs) for MNDs in the US from 1990 to 2021.

Methods: We performed a secondary analysis of MNDs in the US using estimates of prevalence, incidence, and mortality obtained from analyses of the Global Burden of Disease 2021 dataset.

Developing a method to estimate aerosol generation during poultry slaughtering and defeathering in Bangladesh: An experimental study.

Influenza viruses can be aerosolized when slaughtering infected chickens, which increases the risk of zoonotic transmission. We conducted pilot experiments to measure the concentrations of airborne particles <2.5 μm during slaughtering and defeathering of chickens to help identify methods that can minimize workers' exposure to potentially hazardous aerosol particles.

Surgical outcomes from haematoma evacuation for intracerebral haemorrhage in the INTERACT3 study.

Background: There is ongoing controversy as to whether surgical intervention to haematoma evacuation benefits patients with acute intracerebral haemorrhage (ICH). This study aimed to evaluate the association of surgical intervention to evacuate the haematoma and 6-month functional outcome in participants of the third Intensive Care Bundle with Blood Pressure Reduction in Acute Cerebral Haemorrhage Trial (INTERACT3).

Methods: This was a secondary analysis of INTERACT3, which enrolled adults (age ≥18 years) spontaneous ICH patients within 6 h after onset.

Intraoperative Cell Salvage for Revision Hip Arthroplasty: A 24-Year Study of Transfusion Requirements.

Background: Revision hip arthroplasty is associated with significant blood loss and a subsequent need for blood transfusion. Intraoperative cell salvage (ICS) is a method of recovering a patient's blood for autologous transfusion. This potentially reduces the need for allogenic blood transfusion (ABT), thus avoiding associated risk and expense.