Construction and application of a NIR benzothianone-based ratiometric fluorescent probe for visual detection of Hg in biological systems and water samples.

Anal Chim Acta

Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, PR China. Electronic address:

Published: August 2025


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Article Abstract

Background: Mercury ions, a toxic heavy metal, pose a significant threat to environmental health, leading to pollution and accumulation in living organisms.

Results: In this study, we have developed a novel ratiometric near-infrared fluorescent probe for the detection of Hg. The probe TM-ND-Hg demonstrated a remarkable Stokes shift (358 nm), a rapid response time (within 1 min), excellent selectivity, stability, and ratiometric fluorescence changes from green to red. Moreover, the probe enabled the portable detection of Hg through color recognition software, which can discern color alterations.

Significance: The probe has been successfully applied to monitor Hg in living A549 cells, zebrafish, and real water samples, showcasing its potential for environmental and biological monitoring.

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http://dx.doi.org/10.1016/j.aca.2025.344176DOI Listing

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