Performance and mechanism of a novel aerobic phosphorus release/anoxic phosphorus uptake process for simultaneous nitrogen and phosphorus removal.

Bioresour Technol

College of Environmental Engineering, Henan University of Technology, Zhengzhou 450001, China; Zhengzhou International Cooperation Base for Science and Technology on Carbon Neutrality of Organic Solid Waste Conversion, Zhengzhou 450001, China; Henan International Joint Laboratory of Environmental Po

Published: September 2025


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Article Abstract

To investigate the feasibility of a novel Nitritation and Denitrification for Simultaneous Nitrogen and Phosphorus Removal system, a 133-day experiment was conducted. The results showed that combining aerobic phosphorus release capacity with denitrification phosphorus uptake capacity was feasible to achieve N and P removal. The removal efficiencies of TN and TP in ND-SNPR system were 98.16 ± 1.75 % and 81.70 ± 8.03 %. In addition, the phosphorus removal pathway of the system was identified as biological phosphorus removal. It is worth noting that the aerobic phosphorus release phenomenon of the ND-SNPR system occurred simultaneously with the short-range nitrification reaction and PHA storage. Thauera played the main role in the removal of nitrogen and phosphorus in the system. The relative abundance of Ploy-P hydrolases, dominated by ppx and relA, increased significantly with the intensification of the aerobic phosphorus release performance. This study provides a new idea for N and P removal and a reference for shortening the path of N and P removal as well as the phenomenon of aerobic phosphorus release.

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http://dx.doi.org/10.1016/j.biortech.2025.132626DOI Listing

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