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Article Abstract
is a common opportunistic pathogen. The potential efficacy of phage therapy has attracted the attention of researchers, but efficient gene-editing tools are lacking, limiting the study of their biological properties. Here, we designed a type V CRISPR-Cas12a system for the gene editing of phages. We first evaluated the active cutting function of the CRISPR-Cas12a system and discovered that it had a higher gene-cutting efficiency than the type II CRISPR-Cas9 system in three different phages. We also demonstrated the system's ability to precisely edit genes in phages, phages, and phages. Using the aforementioned strategies, non-essential phage genes can be efficiently deleted, resulting in a reduction of up to 5,215 bp (7.05%). Our study has provided a rapid, efficient, and time-saving tool that accelerates progress in phage engineering.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11269290 | PMC |
| http://dx.doi.org/10.1016/j.isci.2024.110210 | DOI Listing |
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