Effectiveness of circulating tumor cells and circulating tumor DNA in peritoneal lavage fluid for predicting metachronous peritoneal metastasis of gastric cancer.

Background: Peritoneal metastasis is a leading cause of death in gastric cancer (GC) patients, highlighting the need for early diagnosis and high-risk population identification. While circulating tumor DNA(ctDNA) and circulating tumor cells (CTC) have been widely studied in blood, their role in peritoneal lavage fluid (PLF) remains unexplored.

Methods: Patients with stage III GC were enrolled, and preoperative/postoperative PLF was collected in this study.

Feasibility of a Novel Robotic Surgical System for Partial Gastrectomy in a Porcine Model.

Background: This preclinical study evaluated the safety and technical feasibility of robot-assisted partial gastrectomy (RPG) using the Carina Platform in a porcine model to accumulate evidence for clinical translation.

Methods: Six pigs underwent RPG (4 with Plan A, 2 with Plan B setups). The acute group (n = 3) were euthanised 1-h postoperation, while the chronic group (n = 3) survived 28 days for recovery assessment.

Predicting Lu-DOTATATE therapy response through immune microenvironment parameters in gastroenteropancreatic neuroendocrine tumors (GEP-NETs).

Background: The prognosis of gastroenteropancreatic neuroendocrine tumors (GEP-NETs) following metastasis is often poor. The efficacy of Lu-DOTATATE therapy and the subgroups that benefit from it remain unclear. Our objective is to characterize the composition of the tumor immune microenvironment in GEP-NETs and to identify predictive biomarkers associated with response and PFS following Lu-DOTATATE.

HiEndo: harnessing large-scale data for generating high-resolution laparoscopy videos under a two-stage framework.

Recent success in generative AI has demonstrated great potential in various medical scenarios. However, how to generate realistic and high-fidelity gastrointestinal laparoscopy videos still lacks exploration. A recent work, Endora, proposes a basic generation model for a gastrointestinal laparoscopy scenario, producing low-resolution laparoscopy videos, which can not meet the real needs in robotic surgery.

Tumor suppressor SLC9A2 inhibits colorectal cancer metastasis and reverses immunotherapy resistance by suppressing angiogenesis.

Background: Colorectal cancer (CRC) is a common and aggressive malignancy that frequently metastasizes to the liver, presenting significant therapeutic challenges. Despite its clinical importance, the mechanisms underlying CRC liver metastasis and resistance to immune therapy remain poorly understood. In this study, we aimed to investigate the molecular mechanisms driving CRC metastasis using a novel approach, which includes the establishment of highly metastatic CRC cell lines.

Clinical study of adebrelimab in combination with apatinib and irinotecan for PD-1 inhibitor-ineffective advanced-stage gastric cancer: study protocol for a single-arm, single-centre, exploratory trial.

Introduction: Immunotherapy has revolutionised cancer treatment. Immune checkpoint inhibitors have demonstrated significant efficacy across multiple tumour types, including gastric cancer, where several approved programmed cell death 1/programmed cell death-ligand 1 (PD-1/PD-L1) inhibitors show promising antitumour activity. While PD-L1 expression serves as a predictive biomarker for PD-1 inhibitor response, and PD-L1-positive patients generally show better outcomes, therapeutic resistance remains a challenge.

DEPP1: A prognostic biomarker linked to stroma‑rich and immunosuppressive microenvironment, promoting oxaliplatin resistance in gastric cancer.

Decidual protein induced by progesterone (DEPP1) was identified to exert heterogeneous functions in several cancers, whereas its role in gastric cancer (GC) remains elusive. In the present study, differential expression analysis was conducted using three Gene Expression Omnibus datasets (GSE54129, GSE26942 and GSE3438). Validation of DEPP1 expression was performed using reverse transcription‑quantitative PCR, western blotting and immunofluorescence.

High extracellular matrix stiffness upregulates TNNT1 to awaken dormant tumor cells in liver metastatic niches of gastric cancer.

Background: Liver is one of the target organs bearing the most frequent distant metastasis of gastric cancer (GC), and patients with GC liver metastasis suffering from poor prognosis. According to "seed and soil" theory, important and complex interactions between disseminated tumor cells (DTCs) and metastasis tumor microenvironment (MTM) have played a vital role in waking the dormant DTCs and promoting their proliferation. We have discovered that the aberrantly activated cancer associated fibroblasts (CAFs) could significantly increase the enrichment and stiffness of extracellular matrix (ECM).

Long-term outcomes and quality of life of laparoscopic-assisted pylorus-preserving gastrectomy compared to laparoscopic-assisted distal gastrectomy with billroth I anastomosis for early gastric cancer(pT1N0M0): A Randomized Controlled Trial.

Objective: Clarify the long-term outcomes of laparoscopic-assisted pylorus-preserving gastrectomy (LAPPG) compared to conventional laparoscopic-assisted distal gastrectomy with billroth I anastomosis (LADGBI) for early gastric cancer(pT1N0M0).

Methods: Patients with cT1N0M0 cancer located in the middle third of the stomach and not suitable for endoscopic submucosal dissection were randomized to undergo LAPPG or LADGBI.

Results: Between August 2017 and October 2019, a total 88 patients (pT1N0M0) were analyzed.

Cav3.1 T-Type Calcium Channel Acts as a Gateway for GABAergic Excitation in the Medial Prefrontal Cortex That Leads to Chronic Psychological Stress Responses in Mice.

Aim: The molecular mechanisms of chronic stress-induced psychiatric disorders, including depression, remain unknown. The current study aimed to assess the role of Cav3.1 T-type calcium channels as a gateway for the chronic stress-induced activation of parvalbumin (PV)-positive gamma-aminobutyric acidergic (GABAergic) neurons in the medial prefrontal cortex (mPFC) of mice.

The PIWI-interacting RNA CRAPIR alleviates myocardial ischemia‒reperfusion injury by reducing p53-mediated apoptosis via binding to SRSF1.

Ischemia-reperfusion (I/R) injury refers to the secondary damage that occurs when blood flow is restored to heart tissues and organs following a period of prolonged ischemia. This damage is exacerbated primarily through mechanisms such as oxidative stress, inflammatory responses and apoptosis, all of which can severely impact patient prognosis. PIWI-interacting RNAs (piRNAs) represent a novel class of small noncoding RNAs that play pivotal roles in regulating gene expression and cellular functions.

Exendin-4 imaging based on gastrointestinal GLP-1R targets for IBD diagnosis and efficacy assessment.

Purpose: Diagnosis of inflammatory bowel disease (IBD) involves history-taking and invasive procedures that primarily evaluate localized intestinal structures. Notably, glucagon-like peptide 1 (GLP-1) and its receptor (GLP-1R) have been established as potent participants in various inflammatory diseases. This study aimed to investigate the potential of molecular imaging targeting GLP-1R in the study of gastrointestinal physiology and disease.

Total laparoscopy-assisted total gastrectomy with Da Vinci robotic system conducted by robotic enhanced neurocomputing joint intelligence gastrointestinal surgery hub (RENJI-GISH): a preliminary clinical study and case report.

Background: On August 7, 2024, the inaugural total laparoscopy-assisted total gastrectomy with the Da Vinci robotic system was performed in the department of gastrointestinal surgery of Renji Hospital, Shanghai Jiaotong University School of Medicine. The procedure, conducted by RENJI-GISH, employed the use of a Da Vinci robot system in conjunction with the Vision Pro and SonoScape medical electronic endoscopy system. This phenomenon has not been documented in the field of gastric cancer surgery The objective of this study is to investigate the safety, feasibility, and surgical effect of the first total laparoscopy-assisted total gastrectomy with the Da Vinci robotic system, conducted by the Robotic Enhanced Neurocomputing Joint Intelligence Gastrointestinal Surgery Hub (RENJI-GISH).

Divergent sex-specific pannexin-1 mechanisms in microglia and T cells underlie neuropathic pain.

Chronic pain is a leading cause of disability, affecting more women than men. Different immune cells contribute to this sexual divergence, but the mechanisms, especially in females, are not well defined. We show that pannexin-1 (Panx1) channels on microglia and T cells differentially cause mechanical allodynia, a debilitating symptom of neuropathic pain.

IFN-γ reprograms cardiac microvascular endothelial cells to mediate doxorubicin transport and influences the sensitivity of mice to doxorubicin-induced cardiotoxicity.

Doxorubicin (DOX) is a first-line chemotherapy agent known for its cardiac toxicity. DOX-induced cardiotoxicity (DIC) severely limits the use for treating malignant tumors and is associated with a poor prognosis. The sensitivity to DIC varies among patients, but the precise mechanisms remain elusive.

Epidemiological characteristics of community-acquired pneumoniae in hospitalized children around COVID-19 from Jiangsu Province, China: a multicenter retrospective study.

Background: It has been reported that the emergence of coronavirus disease 2019 (COVID-19) has changed the epidemiological characteristics of many pathogens, but the epidemiological characteristics of (MP) infection in hospitalized children with community-acquired pneumonia (CAP) are not clear. The aim of this study was to answer this question.

Methods: Children with CAP in three tertiary hospitals (hospitals A, B and C) from 2018 to 2023 were selected.

A Retrospective Study of Laparoscopic Distal Gastrectomy Guided by Carbon Nanoparticle Suspension Injection Lymphography for Gastric Cancer.

BackgroundThe use of lymph node (LN) tracers can help obtain a complete dissection of the LNs and increase the detection rate of metastatic LNs. Carbon nanoparticle suspension injection (CNSI) has become increasingly used in radical gastrectomy procedures. This study is designed to evaluate the quality of LN dissection in gastric cancer patients with laparoscopic distal gastrectomy under the guidance of CNSI lymphography.

Indole-3-Carboxaldehyde Inhibits Inflammatory Response and Lipid Accumulation in Macrophages Through the miR-1271-5p/HDAC9 Pathway.

Indole-3-carboxaldehyde (ICA), a microbiota-derived tryptophan metabolite, has been reported to protect against atherosclerosis. However, the molecular mechanisms for its atheroprotective effect remain largely unknown. This study aimed to explore the influence of ICA on lipid accumulation and inflammatory response in THP-1 macrophage-derived foam cells.

Three-dimensional kinematic analysis of the cervical spine following posterior atlantoaxial fusion under physiological loading: An in vivo study.

Background: This study aimed to analyze the three-dimensional cervical motion characteristics in patients who underwent posterior atlantoaxial fusion surgeries using cone beam computed tomography and 3D3D registration technology.

Methods: The study selected 20 patients who underwent posterior atlantoaxial fusion surgery and 20 healthy people as the control group. All subjects underwent cone beam computed tomography scans of the occipital and cervical spine in 7 different functional positions, then 3D3D registration of Occipital-C7 was performed at each functional position to calculate the motion characteristics of each segment.

An in vivo 3-dimensional kinematics study of the cervical vertebrae under physiological loads in patients with cervical spondylosis.

Background Context: Studies of in vivo kinematic differences between healthy individuals and those with cervical spondylosis (CS) have been reported, but only movements under nonphysiological loads have been investigated. Differences in the in vivo, cervical kinematics between healthy individuals and those with CS are unknown.

Purpose: To investigate the in vivo, cervical kinematics of patients with CS under physiological loads.

Image-based deep learning model to predict stoma-site incisional hernia in patients with temporary ileostomy: A retrospective study.

The prophylactic implantation of biological mesh can effectively prevent the occurrence of stoma-site incisional hernia (SSIH) in patients undergoing stoma retraction. Therefore, our study prospectively established and validated a mixed model, which combined radiomics, stepwise regression, and deep learning for the prediction of SSIH in patients with temporary ileostomy. The mixed model showed good discrimination of the SSIH patients on all cohorts, which outperformed deep learning, radiomics, and clinical models alone (overall area under the curve [AUC]: 0.

Effectiveness of peritoneal lavage fluid circulating tumour cells and circulating tumour DNA in the prediction of metachronous peritoneal metastasis of gastric cancer (pT4NxM0/pT1-3N+M0) after radical resection: protocol of a prospective single-centre clinical study.

Background: Gastric cancer (GC) is the fourth leading cause of cancer mortality worldwide. Peritoneal metastasis (PM) is a significant cause of death in patients with GC, and presents a major challenge in clinical diagnosis and treatment. Predicting the occurrence of PM in high-risk patients, and diagnosing and treating PM in advance to improve patient survival, remains an unsolved problem in clinical practice.