The Roles of GABA and NMDA Receptors in Viral Infections: Based on current literature.

Gamma-aminobutyric acid receptors (GABARs) primarily function by suppressing inflammatory responses, modulating neuronal excitability, and maintaining intracellular homeostasis, whereas N-methyl-D-aspartate receptors (NMDARs) play a key role in mediating pathological processes through the regulation of excitatory neurotransmission and immune responses. Viral infections have the capacity to modify the expression and functionality of these receptors, either directly or indirectly, thereby contributing to dysregulation within the neurological and immune systems and triggering a range of disease states. This review offers a comprehensive analysis of the mechanisms through which various viral infections interact with GABARs and NMDARs, emphasizing the possible intricate roles these receptors play in viral pathogenesis.

Enhanced nitrate removal by ternary electron donors of solid carbon source, pyrite, and iron-scrap in stormwater bioretention systems: Impact of natural and synthetic carbon sources.

Insufficient availability of electron donors is challenging for nitrate removal in bioretention systems when treating carbon-limited stormwater runoff. This study constructed two ternary electron-donor systems incorporating solid carbon source, pyrite, and iron-scrap to enhance mixotrophic denitrification, and investigated the impacts of natural carbon source (woodchip) and synthetic carbon source (polycaprolactone) on denitrification performance and by-product generation. Results showed that both systems exhibited stable and efficient nitrate removal (85.

A weakly-supervised oriented object detector : Knowledge-based dropblock and unified regression network.

Object detection in remote sensing images (RSIs) is facilitated by oriented bounding boxes, yet rotated boxes (RBoxes) are typically more labor-intensive than horizontal boxes (HBoxes). Consequently, there is a tendency in most research to explore HBox-based weakly-supervised detectors with self-supervised constraints on spatial transformations. However, such weakly-supervised networks for HBoxes tend to focus on the most discriminative parts of objects, which can adversely affect the network's location accuracy.

Integrated study on the genome, transcriptome, and metabolome of Gelsemium elegans and mining of related enzyme genes involved in koumine biosynthesis.

Gelsemium elegans a plant of the Loganiaceae family, is highly toxic and contains many alkaloids of potential pharmacological value. Koumine, is the most abundant component of G. elegans, and belongs to monoterpene indole alkaloids and is valued in medical research as it has important anti-inflammatory and anxiolytic properties.

Development of innovative light-curing resin composites featuring dual hydrolysis resistance.

Objective: The present study aims to address the negative impact of oral wet environments on the performance of light-curing composite resins by developing novel light-curing resin composites featuring dual hydrolytic resistance barriers. We evaluate their mechanical properties and hydrolysis resistance compared to Z250XT.

Methods: Urushiol-modified monomer (UMM) and Glass flake-KH570 were synthesized and incorporated into resin composites.

Rethinking cell-based neural architecture search: A theoretical perspective.

In this paper, we explore several fundamental theoretical issues in cell-based neural architecture search, including whether different architectures in search space are equally important in terms of the minimal training loss they can achieve, and whether an optimal cell found by architecture search is still optimal when using it in a cell stack. We model the directed acyclic graph (DAG) of a cell as a stem path plus multiple bypass edges. For single cells, we prove that the training loss is always decreased or at least maintained if skip connections are added into bypass edges in a greedy order and learnable operations are added into bypass edges in any order.

Hypoxia tolerance determine differential gelsenicine-induced neurotoxicity between pig and mouse.

Background: Gelsemium elegans (G. elegans) is widely recognized as one of the most toxic plants globally, particularly harmful to humans. Some reports indicate that it is non-toxic to pigs and even has a growth-promoting effect; however, the underlying reasons for this paradox remain unclear.

Experimental and Computational Investigation of the Target and Mechanisms of Alkaloids in the Central Nervous System.

has a long history of medicinal use but is also a poisonous plant. Some low-toxicity alkaloids in exhibit anxiolytic, anti-inflammatory, analgesic, and other pharmacological effects; however, certain alkaloids in are highly toxic. Nevertheless, the molecular targets underlying the biological effects of alkaloids remain poorly understood.

Pharmacological effects of koumine on acute lung injury in septic mice: From in vivo experiments and network pharmacology studies.

Acute lung injury (ALI) caused by sepsis is one of the most common critical diseases, which is difficult to treat and has a high fatality rate. Koumine is one of the main active components of Gelsemium plants and has been confirmed to have potential for drug development; however, its therapeutic effects on ALI have not yet been studied. This study established ALI due to sepsis using cecal ligation and puncture (CLP) and assessed the therapeutic effects of koumine by measuring mouse survival rates, lung tissue pathological damage, inflammatory factors, and oxidative stress levels.

Cascade Amplifying Electrochemical Bioanalysis for Zearalenone Detection in Agricultural Products: Utilizing a Glucose-Fenton-HQ System on Bimetallic-ZIF@CNP Nanocomposites.

The integration of advanced materials and signaling cascade strategies is a promising and highly relevant topic for enhancing the performance of bioanalysis. In this study, a three-stage cascade amplification electrochemical bioanalysis (TCAE-bioanalysis) was developed and evaluated for the detection of zearalenone (ZEN). This method couples immunoreaction with a glucose-Fenton-hydroquinone (HQ) system on bimetallic-ZIF (B-ZIF)@CNP nanocomposites.

Effects of antibiotic and disinfectant exposure on the mouse gut microbiome and immune function.

Unlabelled: This study explores the effects of disinfectant and antibiotic exposure on gut health, focusing on gut microbiota balance and gut immune function. Our analysis indicates that disinfectants increase the proportion of Gram-positive bacteria, particularly increasing levels, while antibiotics increase the proportion of Gram-negative bacteria, especially levels. These changes disrupt microbial harmony and affect the gut microbiome's functional capacity.

Effect of cytochrome P450 3A4 on tissue distribution of humantenmine, koumine, and gelsemine, three alkaloids from the toxic plant Gelsemium.

Humantenmine, koumine, and gelsemine are three indole alkaloids found in the highly toxic plant Gelsemium. Humantenmine was the most toxic, followed by gelsemine and koumine. The aim of this study was to investigate and analyze the effects of these three substances on tissue distribution and toxicity in mice pretreated with the Cytochrome P450 3A4 (CYP3A4) inducer ketoconazole and the inhibitor rifampicin.

An efficient method for the preparative isolation and purification of alkaloids from by using high speed counter-current chromatography and preparative HPLC.

We established an efficient method using high-speed countercurrent chromatography (HSCCC) combined with preparative high-performance liquid chromatography (prep-HPLC) for isolating and purifying Gels () alkaloids. First, the two-phase solvent system composed of 1% triethylamine aqueous solution/-hexane/ethyl acetate/ethanol (volume ratio 4:2:3:2) was employed to separate the crude extract (350 mg) using HSCCC. Subsequently, the mixture that resulted from HSCCC was further separated by Prep-HPLC, resulting in seven pure compounds including: 14-hydroxygelsenicine (, 12.

Comparative Analysis of the Gelsemium Alkaloids Metabolism in Human, Pig, Goat, and Rat Liver Microsomes.

Aim: The aim of this study was to investigate the metabolism of in human, pig, goat and rat liver microsomes and to elucidate the metabolic pathways and cleavage patterns of the Gelsemium alkaloids among different species.

Methods: A human, goat, pig and rat liver microsomes were incubated . After incubating at 37°C for 1 hour and centrifuging, the processed samples were detected by was used to detect alcohol extract of and its metabolites.

Reference Genes Screening and Gene Expression Patterns Analysis Involved in Gelsenicine Biosynthesis under Different Hormone Treatments in .

Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is an accurate method for quantifying gene expression levels. Choosing appropriate reference genes to normalize the data is essential for reducing errors. is a highly poisonous but important medicinal plant used for analgesic and anti-swelling purposes.

China Medicinal Plants of the -A Review of Their Botanical Characteristics, Use, Phytochemistry, Active Pharmacological Components, and Toxicology.

(AG) is mainly distributed in Chinese provinces and areas south of the Yangtze River Basin. It is mostly concentrated or scattered in mountainous bushes or woods with high humidity. Approximately 57 chemical components of AG have been identified, including flavonoids, phenols, steroids and terpenoids, volatile components, and other chemical components.

Antinociceptive effect of gelsenicine, principal toxic alkaloids of gelsemium, on prostaglandin E2-induced hyperalgesia in mice: Comparison with gelsemine and koumine.

Gelsemium elegans (G.elegans) is a plant of the Loganiaceae family, known for its indole alkaloids, including gelsemine, koumine, and gelsenicine. Gelsemine and koumine are well-studied active alkaloids with low toxicity, valued for their anti-anxiety and analgesic properties.

DNA-bridged double gold nanoparticles-based immunochromatography for dual-mode detection of ochratoxin A.

A novel immunochromatography strip (ICS) based on the DNA-bridged double gold nanoparticles was established and evaluated for the dual-mode detection of ochratoxin A (OTA). For this purpose, the streptavidin was coupled with the big size of gold nanoparticle (40 nm, AuNP), the OTA monoclonal antibody and biotinylated DNA-SH were simultaneously immobilized on the small size of AuNP (20 nm), and then the enhanced ICS was self-assembled and evaluated. The dual-mode detection of semi-quantification and quantification had been achieved and performed by the proposed ICS.

Effect of Dietary Benth. Extract on the Growth, Slaughter Performance, Meat Quality, Intestinal Morphology, and Microflora of Yellow-Feathered Chickens.

The plant species Benth. (GEB) promotes pig and sheep growth; however, little is known about its effects in chickens. In this study, a GEB extract (GEBE) was prepared, and its effects on the growth, slaughter, antioxidant performance, meat quality, serum biochemical indices, intestinal morphology, and microflora of yellow-feathered chickens were evaluated.

Protocol to characterize target components of Gelsemium using an in-house herb database and high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry.

Currently, the identification of herb metabolites is challenging due to a lack of clear standards. Here, using Gelsemium as an example, we present a protocol for characterizing target components of herbs. This approach utilizes high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry guided by an in-house herb metabolite database based on reported studies and mass spectrometry.

Room Temperature Anhydrous Suzuki-Miyaura Polymerization Enabled by C-S Bond Activation.

The Suzuki-Miyaura cross-coupling is one of the most important and powerful methods for constructing C-C bonds. However, the protodeboronation of arylboronic acids hinder the development of Suzuki-Miyaura coupling in the precise synthesis of conjugated polymers (CPs). Here, an anhydrous room temperature Suzuki-Miyaura cross-coupling reaction between (hetero)aryl boronic esters and aryl sulfides was explored, of which universality was exemplified by thirty small molecules and twelve CPs.

A Novel Two-Dimensional Liquid Chromatography Combined with Ultraviolet Detection Method for Quantitative Determination of Pyridoxal 5'-Phosphate, 4-Pyridoxine Acid and Pyridoxal in Animal Plasma.

Vitamin B is an indispensable micronutrient in organisms and is widely distributed in blood, tissues, and organs. Changes in the content and ratio of vitamin B6 can affect the entire physiological condition of the body, so it becomes particularly important to reveal the relationship between changes in its content and disease by monitoring vitamin B6 levels in the organism. In this study, a two-dimensional liquid chromatography-UV detector (2D-LC-UV) was used to establish a method for the simultaneous detection of PLP, PA, and PL for the first time.

Long-term plant diversity increases soil extractable organic carbon and nitrogen contents in a subtropical forest.

Plant diversity is one of the various factors influencing ecosystem functions such as soil carbon (C) and nitrogen (N) stocks. Soil extractable organic carbon (EOC) and nitrogen (EON) contents are active fractions in soil organic matter, but little is known about the impact of variations in long-term plant diversity on soil EOC and EON contents in forest ecosystems. Utilizing the Biodiversity-Ecosystem Functioning Experiment China platform, we selected long-term plant diversity level treatments, distinguished the functional types of evergreen and deciduous plants, and explored their effects on soil EOC and EON contents.

Effect of different bone cement distributions in percutaneous kyphoplasty on clinical outcomes for osteoporotic vertebral compression fractures: A retrospective study.

Osteoporotic fractures and their complications are becoming increasingly harmful to the elderly. This study aimed to evaluate the clinical results of connected or unconnected bilateral cement after bilateral percutaneous kyphoplasty (PKP) in patients with osteoporotic vertebral compression fractures (OVCF). The clinical data of 217 patients with single-segment OVCF were retrospectively collected.