Applying next-generation sequencing to detect microsatellite instability in pan-cancer patients: a retrospective study of 35,563 Chinese cases.

Microsatellite instability (MSI) serves as an important therapeutic and prognostic marker. Immunohistochemistry (IHC) and polymerase chain reaction (PCR) have long been regarded as the "gold standard" for MSI detection. In recent years, next-generation sequencing (NGS)-based methods, offering expanded target coverage of microsatellite (MS) loci and improved analytical performance, have gained widespread acceptance.

Enhancing cardiac MRI reliability at 3 T using motion-adaptive B shimming.

Purpose: Magnetic susceptibility differences at the heart-lung interface introduce B-field inhomogeneities that challenge cardiac MRI at high field strengths (≥ 3 T). Although hardware-based shimming has advanced, conventional approaches often neglect dynamic variations in thoracic anatomy caused by cardiac and respiratory motion, leading to residual off-resonance artifacts. This study aims to characterize motion-induced B-field fluctuations in the heart and evaluate a deep learning-enabled motion-adaptive B shimming pipeline to mitigate them.

mSEPT9 performs better than CEA in NAT response and MRD assessment and recurrence prediction of stage III CRC.

Aims: This study aimed to investigate the capability of methylated SEPT9 (mSEPT9) assay in assessing the neoadjuvant therapy (NAT) response,the minimal residual disease (MRD) and predicting the recurrence of stage III colorectal cancer (CRC).

Materials & Methods: We prospectively recruited 124 stage III CRC patients. All patients received pre-surgical NAT and subsequent curative surgery, and were followed up to 627 days.

Visualization analysis of CBL application in Chinese and international medical education based on big data mining.

Objective: To employ big data mining to provide a visualization analysis of Case-Based Learning (CBL) application in Chinese and international medical education, with the aim of observing the potential applications of CBL.

Methods: All included literature was obtained from the Web of Science (WoS) core collection database, Chinese core periodicals database, Chinese Social Sciences Citation Index (CSSCI), Chinese Science Citation Database of China National Knowledge Infrastructure (CNKI), Wangfang Database, and CQVIP Database. CiteSpace software (6.

Improving fat saturation robustness in outer extremity MRI with a local shim coil insert.

Purpose: High-quality fat suppression is essential for various MRI applications. In musculoskeletal imaging, poor fat suppression caused by severe B inhomogeneity can obscure important lesions, potentially leading to inaccurate diagnoses. This problem is particularly exacerbated in off-isocenter imaging, where conventional shimming using second-order spherical harmonic shim coils often proves inadequate due to elevated B inhomogeneity.

B Magnetic Field Conditions in the Human Heart at 3 T Across One Thousand Subjects: A Numerical Simulation Study.

Functional scans in cardiovascular magnetic resonance (CMR) adopting bSSFP sequences suffer from dark band artifacts due to B inhomogeneity. The best remedy to mitigate this issue is through cardiac B shimming. The development of an optimal B shim strategy for the human heart is hindered by a limited understanding of B conditions in clinical diagnostic orientations of CMR.

Comparative effectiveness of various teaching modes, including PBL, CBL, and CTTM in paediatric medical education with combined online and offline approaches.

Objective: To explore the differences in the effectiveness of various teaching methods combining online and offline modes in paediatric medical education.

Methods: Fourth-year medical paediatric students at our university in 2020 were randomly divided into three groups: a control group, experimental group 1, and experimental group 2, with 30 students in each group. The control group received traditional teaching, experimental group 1 received a combination of online and offline teaching using two methods simultaneously, and experimental group 2, in addition to the methods used in experimental group 1, engaged in real-case teaching in the ward.

Nerve Growth Factor from Pancreatic Cancer Cells Promotes the Cancer Progression by Inducing Nerve Cell-Secreted Interleukin-6.

Pancreatic cancer (PC) is a cancer with a poor prognosis, and nerve growth factor (NGF) is involved in the pathogenesis of PC within the unknown exact role. Herein, SW1990 cells and PC12 cells were co-cultured using transwell co-culture system and subsequently revealed that NGF was overexpressed in SW1990 cells and promoted PC12 cell proliferation. Knockdown of NGF expression in SW1990 cells using lentiviral shRNA effectively inhibited NGF expression in SW1990 cells and reduced its stimulatory effect on PC12 cell proliferation.

Advancements in MR hardware systems and magnetic field control: B shimming, RF coils, and gradient techniques for enhancing magnetic resonance imaging and spectroscopy.

High magnetic field homogeneity is critical for magnetic resonance imaging (MRI), functional MRI, and magnetic resonance spectroscopy (MRS) applications. B inhomogeneity during MR scans is a long-standing problem resulting from magnet imperfections and site conditions, with the main issue being the inhomogeneity across the human body caused by differences in magnetic susceptibilities between tissues, resulting in signal loss, image distortion, and poor spectral resolution. Through a combination of passive and active shim techniques, as well as technological advances employing multi-coil techniques, optimal coil design, motion tracking, and real-time modifications, improved field homogeneity and image quality have been achieved in MRI/MRS.

Preliminary study on the active substances and cellular pathways of lactic acid bacteria for colorectal cancer treatment.

Colorectal cancer (CRC) is a common malignant tumor and is one of the three most common cancers worldwide. Traditional surgical treatment, supplemented by chemotherapy and radiotherapy, has obvious side effects on patients. Immunotherapy may lead to some unpredictable complications.

Fabrication of a 25.2 T NMR magnet for an extreme condition user facility in China.

This article highlights a research study on the fabrication of a 25.2 T ultra-high field NMR magnet for an extreme condition user facility in China.

Comprehensive genomic profiling reveals prognostic signatures and insights into the molecular landscape of colorectal cancer.

Background: Colorectal cancer (CRC) is a prevalent malignancy with diverse molecular characteristics. The NGS-based approach enhances our comprehension of genomic landscape of CRC and may guide future advancements in precision oncology for CRC patients.

Method: In this research, we conducted an analysis using Next-Generation Sequencing (NGS) on samples collected from 111 individuals who had been diagnosed with CRC.

Molecular pathology and clinical treatment of independent HPV primary serous carcinoma of the uterine cervix (USCC): A case report.

On October 23, 2020, a 69-year-old Chinese female patient was admitted to Yuncheng Hospital due to a history of postmenopausal bleeding and lower abdominal pain for 5 months. The HPV test and pathology results indicated the presence of independent HPV in primary serous carcinoma of the uterine cervix. The genetic testing identified variants of uncertain significance (PAX8 p.

High resolution simulation and measurement demonstrate oscillatory spatiotemporal B fluctuations across the human cardiac cycle.

Purpose: Functional cardiac MRI scans employing balanced steady-state free precession sequences suffer from dark band artifacts in the myocardium due to B inhomogeneity. We recently introduced a novel method for the theoretical derivation of B distributions in the human heart. This study aims to simulate the B distributions in the heart across the cardiac cycle using structural MR images and validate the simulations via in vivo measured cardiac phase-specific B maps on the same subjects at 3T.

Risk Factors and Prediction Methods for Endometriosis Combined with Ureteral Stricture Based on Logistic-Regression Analysis.

Objective: This study aimed to explore the risk factors of patients with endometriosis (EMS) and ureteral stricture and to establish a prediction model based on logistic-regression analysis.

Methods: The clinical data of 228 EMS patients in Jiaozhou Central Hospital of Qingdao from May 2019 to May 2022 were selected for a retrospective study. According to the results of ureteroscopic biopsy, they were divided into concurrent (n = 32) and nonconcurrent (n = 196) groups.

PDLIM2 can inactivate the TGF-β/Smad pathway to inhibit the malignant behavior of ovarian cancer cells.

PDZ-LIM domain-containing Protein 2 (PDLIM2) has been reported to be downregulated in ovarian cancer. However, its exact function and mechanism in regulating ovarian cancer progression have not been elucidated. This work researched the exert effect and mechanism of PDLIM2 on ovarian cancer progression.

Design and realization of a multi-coil array for B field control in a compact 1.5T head-only MRI scanner.

Purpose: To design and implement a multi-coil (MC) array for B field generation for image encoding and simultaneous advanced shimming in a novel 1.5T head-only MRI scanner.

Methods: A 31-channel MC array was designed following the unique constraints of this scanner design: The vertically oriented magnet is very short, stopping shortly above the shoulders of a sitting subject, and includes a window for the subject to see through.

The value of ultrasonography combined with carbohydrate antigen 125 and 19-9 detection in the diagnosis of borderline ovarian tumors and prediction of recurrence.

Objective: This study aimed to investigate the clinical value of ultrasonography combined with tumor markers in the diagnosis and prediction of recurrence of borderline ovarian tumors (BOTs) and analyze the value of the combination of two different auxiliary examinations in the diagnosis and prediction of recurrence of BOTs.

Methods: Here, 221 patients with BOTs confirmed by postoperative pathology were enrolled. Their clinical data, including the ultrasonography features, tumor markers, and clinicopathological data, were retrospectively analyzed.

Reciprocal inhibition of expression between RAV1 and BES1 modulates plant growth and development in Arabidopsis.

RAV1 (Related to ABI3/VP1) is a plant-specific B3 and AP2 domain-containing transcription factor that acts as a negative regulator of growth in many plant species. The expression of RAV1 is downregulated by brassinosteroids (BRs); large-scale transcriptome analyses have shown that the expression of RAV1 was previously targeted by BRI1-EMS-SUPPRESOR1 (BES1) and BRASSINAZOLE-RESISTANT1 (BZR1), which are critical transcription factors for the BR-signaling process. Using RAV1-overexpressing transgenic plants, we showed that RAV1 overexpression reduced the BR signaling capacity, resulting in the downregulation of BR biosynthetic genes and BES1 expression.

Analysis of serum homocysteine concentration in patients less than 35 years of age with polycystic ovary syndrome and hyperandrogenism.

Objectives: An increase in homocysteine (Hcy) concentration is closely related to polycystic ovary syndrome (PCOS). This study aimed to further explore serum homocysteine concentration and its influencing factors in clinically young (≤ 35 years) patients with PCOS and hyperandrogenism.

Material And Methods: An electrochemical immunoassay was used to investigate the changes in serum homocysteine and related indexes in clinically young patients with PCOS and hyperandrogenism, and the results were statistically analyzed.

The Promising Nanovectors for Gene Delivery in Plant Genome Engineering.

Highly efficient gene delivery systems are essential for genetic engineering in plants. Traditional delivery methods have been widely used, such as Agrobacterium-mediated transformation, polyethylene glycol (PEG)-mediated delivery, biolistic particle bombardment, and viral transfection. However, genotype dependence and other drawbacks of these techniques limit the application of genetic engineering, particularly genome editing in many crop plants.

Clinical Analysis of the Discovery of Malignant Gynecological Tumors in the Diagnosis and Treatment of Pelvic Organ Prolapse.

Background: Clinically, malignant gynecological tumors found by chance during the diagnosis and treatment of pelvic organ prolapse (POP) are rare, and they are usually missed, leading to delayed diagnosis and treatment. The initial treatment of these tumors cannot be standardized, and, as a single surgical intervention may not be able to treat both the tumor and prolapse, secondary surgery is usually needed, affecting the quality of life of patients.

Case Presentation: The present study retrospectively analyzed the data of three patients who were diagnosed with malignant gynecological tumors during the diagnosis and treatment of POP.