Network Pharmacological Analysis of TCM Formulae Based on the Syndrome Ontology and Multidimensional Quantitative Association Computing Platform.

Current network pharmacology methods for TCM formulae predict potential active ingredients and mechanisms of action for specific diseases by constructing a formula-ingredient-target-pathway-disease network. Syndrome differentiation and treatment, a core principle of TCM, embodies its holistic and dynamic approach, emphasizing individual variability and disease progression. By integrating syndrome characteristics into this network, researchers can develop multi-target drugs tailored to each disease stage, enabling precise and personalized treatment.

USP36 suppresses breast cancer cell ferroptosis by regulating the ubiquitination and stability of KIF2C.

Breast cancer is the most common malignancy in women, with KIF2C acting as an oncogene. However, its role in ferroptosis and posttranslational regulation in BC cells is unclear. Our study analyzed KIF2C expression in BC tissues using bioinformatics and found it overexpressed, correlating with poorer survival.

The effect of the displacement pressure on migration of fluid during the imbibition for tight oil reservoir.

In recent years, tight oil has emerged as a significant and complex topic in the fields of oil exploration and development. Following hydraulic fracturing, wells are typically shut-in for a designated period to facilitate water uptake into shale formations, which has become the predominant method for developing tight oil resources. During this process, initial pressure-specifically the shut-in pressure post-fracturing-plays a crucial role in ensuring imbibition recovery and determining shut-in duration.

Ground-Active Arthropod Diversity Under Energycane and Biomass Sorghum Production.

Energycane and biomass sorghum are two of the most promising cellulosic energy crops in the southeastern US. Research on these two energy crops has focused mainly on biomass production, and there is a lack of knowledge on their ability to promote biodiversity and ecosystem services. This paper presents results from a comprehensive study on ground-active arthropod diversity in seven sites across five states in the southeastern US (Florida, Georgia, Louisiana, Mississippi, and Texas).

Small regulatory RNAs as key modulators of antibiotic resistance in pathogenic bacteria.

The escalating antibiotic resistance crisis poses a significant challenge to global public health, threatening the efficacy of current treatments and driving the emergence of multidrug-resistant pathogens. Among the various factors associated with bacterial antibiotic resistance, small regulatory RNAs (sRNAs) have emerged as pivotal post-transcriptional regulators which orchestrate bacterial adaptation to antibiotic pressure via diverse mechanisms. This review consolidates the current knowledge on sRNA-mediated mechanisms, focusing on drug uptake, drug efflux systems, lipopolysaccharides, cell wall modification, biofilm formation, and mutagenesis.

Spontaneous carotid-cavernous sinus fistula with fibromuscular dysplasia: illustrative case.

Background: A carotid artery cavernous sinus fistula (CCF) is an abnormal shunt of blood vessels, allowing blood to flow directly or indirectly from the carotid artery into the cavernous sinus. The most common cause of spontaneous CCF (s-CCF) is ruptured internal carotid-cavernous sinus aneurysms, while fibromuscular dysplasia (FMD) is rare. FMD is a rare, idiopathic, segmental, noninflammatory, and nonatherosclerotic disease of medium-sized arteries, characterized by abnormal cell proliferation and distortion of arterial wall architecture.

Drug functional remapping: a new promise for tumor immunotherapy.

The research and development of new anti-cancer drugs face challenges such as high costs, lengthy development cycles, and limited data on side effects. In contrast, the clinical safety and side effects of traditional drugs have been well established through long-term use. The development or repurposing of traditional drugs with potential applications in cancer treatment offers an economical, feasible, and promising strategy for new drug development.

Activation of ferritin light chain (FTL) by transcription factor salmonella pathogenicity island 1 modulates glycolysis to drive metastasis of ovarian cancer cells.

Background: Ovarian cancer (OC) is the most lethal gynecological cancer often diagnosed at an advanced stage due to a lack of effective biomarkers. Ferritin light chain (FTL) is implicated in the development of various cancers, but its impact on OC remains unknown.

Research Design And Methods: Bioinformatics methods were utilized to analyze FTL.

Modulating the tumoral SPARC content to enhance albumin-based drug delivery for cancer therapy.

Insufficient delivery of therapeutic agents into solid tumors by systemic administration remains a major challenge in cancer treatment. Secreted protein acidic and rich in cysteine (SPARC) has high binding affinity to albumin and has been shown to enhance the penetration and uptake of albumin-based drug carriers in tumors. Here, we developed a strategy to alter the tumor microenvironment (TME) by upregulating SPARC to enhance the delivery efficiency of albumin-based drug carriers into tumors.

Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer.

Nuclear factor kappa-B (NF-κB), a pivotal transcriptional regulator, plays a crucial role in modulating downstream genes implicated in tumor drug resistance. We establish a programmable system within bladder cancer cells to tailor drug responses by employing a synthetic clustered regularly interspaced short palindromic repeats (CRISPR)-based expression strategy that emulates natural transcriptional regulators. Our investigation uncovers the functional significance of Opa-interacting protein 5 (OIP5), upregulated upon NF-κB activation, as a key regulator governing drug-resistance to vincristine (VCR) treatment in bladder cancer.

Prognostic impact of lymphovascular and perineural invasion in squamous cell carcinoma of the tongue.

This study aimed to investigate the prognostic impact of lymphovascular and perineural invasions in patients with squamous cell carcinoma of the tongue who received surgery-based treatment at our institution between January 2013 and December 2020. Patients were divided into four groups based on the presence of perineural (P-/P +) and lymphovascular invasions (V-/V +): P-V-, P-V + , P + V-, and P + V + . Log-rank and Cox proportional hazard models were used to evaluate the association between perineural /lymphovascular invasion and overall survival (OS).

Modified β-cyclodextrin microspheres towards the application in intumescent fire resistance and smoke-suppressing of bio-based poly(L-lactic acid).

In this work, the β-cyclodextrin (β-CD) was modified by a phosphazene compound to prepare a novel amorphous derivate (β-CDCP), which was combined with the ammonium polyphosphate (APP) as a synergistic flame retardant (FR) of the bio-based poly(L-lactic acid) (PLA). The effects of the APP/β-CDCP on the thermal stability, combustion behavior, pyrolysis process, fire resistance performance and crystallizability of the PLA were investigated comprehensively and in depth by thermogravimetric (TG) analysis, limited oxygen index (LOI) analysis, UL-94 test, cone calorimetry measurement, TG-infrared (TG-IR), scanning electron microscopy-energy dispersive spectrometer, Raman spectroscopy, pyrolysis-gas chromatography/mass spectrometry and differential scanning calorimetry. The PLA/5%APP/10%β-CDCP showed a highest LOI of 33.

Hybrid Micelles of Carbon Quantum Dot-Doxorubicin Conjugates as Nanotheranostics for Tumor Therapy and Turn-On Fluorescence Imaging: Impact of Conjugated Structures and On-Off-On Mechanism.

Carbon quantum dots (CDs) have attracted more and more attention in the field of biological imaging, while their applications are restricted due to their nonspecific fluorescence and small particle size. Herein, two pH-responsive carbon quantum dot-doxorubicin (DOX) conjugates were designed with maleic acid (MA, -butenedioic acid) and fumaric acid (FA, -butenedioic acid) as linker, respectively, which could self-assemble into unique hybrid micelles as tumor-specific carrier-free nanotheranostics. Owing to the acid-labile covalent modification with conjugated groups and the interaction with the surrounding DOX molecules, the fluorescence of CDs was completely quenched, while it could be recovered in the tumor intracellular microenvironment by acid-triggered cleavage of the fluorophore-drug conjugates, showing excellent turn-on fluorescence for effective cellular imaging.

Triclosan to Improve the Antimicrobial Performance of Universal Adhesives.

To solve the proble ms of composite restoration failure caused by secondary caries, this study reports a light curable antibacterial triclosan derivative (TCS-IH), which was synthesized and added to the existing commercial universal adhesive to achieve a long-term antibacterial effect The effect of mixing different mass percentages of TCS-IH on the bond strength of dentin was also investigated.TCS-IH was synthesized by solution polymerization and characterized by nuclear magnetic resonance hydrogen spectroscopy (1H NMR) and Fourier transform infrared (FTIR) spectroscopy. Two commercial universal adhesives, Single Bond Universal and All Bond Universal, were selected and used as the control group, and universal adhesives with different mass percentages (1 wt%, 3 wt%, 5 wt% and 7 wt%) of TCS-IH were used as the experimental group.

A novel prognostic system combining carbonic anhydrase II and preoperative CA19-9 for intrahepatic cholangiocarcinoma after curative resection.

Background: The role of carbonic anhydrase II (CAII) in intrahepatic cholangiocarcinoma (ICC) was investigated and a novel prognostic system combining CAII and preoperative carbohydrate antigen 19-9 (CA19-9) was established to predict the survival of patients with ICC after curative resection.

Methods: A total of 110 patients who underwent curative-intent resection for ICC between 2012 and 2020 were retrospectively analyzed. CAII in tumor and peritumor regions was examined by immunohistochemistry, and the relationships between clinicopathological factors and the prognostic value of CAII and CA19-9 were analyzed.

[Evaluation of fluid responsiveness in patients with septic shock by critical ultrasound with small dose fluid challenge].

Objective: To explore the value of critical ultrasound in evaluating the fluid responsiveness of small dose volume challenge in patients with septic shock.

Methods: Thirty-six patients with septic shock admitted to the Third People's Hospital of Datong from January 2021 to December 2021 were enrolled, and the patients were randomly divided into control group and observation group, with 18 patients in each group. The control group was treated with traditional fluid challenge (500 mL of crystalloid injected within 30 minutes); the observation group received a small dose fluid challenge (100 mL of crystalloid injected within 1 minute).

Biomimetic Mineralized Hydrophilic Polyurethane Primers for Inducing Dentin Tubule Fillings.

This experiment aimed to synthesize a biomimetic mineralized hydrophilic polyurethane dentin primer containing DDDEEKC peptide (DDDEEKC-PU) to fill dentin tubules and induce mineralization. The degree of conversion (DC) was tested. Dentin samples were acid-etched and treated with DDDEEKC-PU.

Effect of Co-culture of mesenchymal stem cell and glomerulus endothelial cell to promote endothelialization under optimized perfusion flow rate in whole renal ECM scaffold.

In recent era, many researches on implantable bio-artificial organs has been increased owing to large gap between donors and receivers. Comprehensive organ based researches on perfusion culture for cell injury using different flow rate have not been conducted at the cellular level. The present study investigated the co-culture of rat glomerulus endothelial cell (rGEC) and rat bone marrow mesenchymal stem cells (rBMSC) to develop micro vascularization in the kidney scaffolds culturing by bioreactor system.

Clinical Eosinophil-Associated Genes can Serve as a Reliable Predictor of Bladder Urothelial Cancer.

Numerous studies have shown that infiltrating eosinophils play a key role in the tumor progression of bladder urothelial carcinoma (BLCA). However, the roles of eosinophils and associated hub genes in clinical outcomes and immunotherapy are not well known. BLCA patient data were extracted from the TCGA database.

Clinical M2 Macrophage-Related Genes Can Serve as a Reliable Predictor of Lung Adenocarcinoma.

Background: Numerous studies have found that infiltrating M2 macrophages play an important role in the tumor progression of lung adenocarcinoma (LUAD). However, the roles of M2 macrophage infiltration and M2 macrophage-related genes in immunotherapy and clinical outcomes remain obscure.

Methods: Sample information was extracted from TCGA and GEO databases.

Phenotypic variation and genome-wide association studies of main culm panicle node number, maximum node production rate, and degree-days to heading in rice.

Background: Grain yield is a complex trait that results from interaction between underlying phenotypic traits and climatic, edaphic, and biotic variables. In rice, main culm panicle node number (MCPNN; the node number on which the panicle is borne) and maximum node production rate (MNPR; the number of leaves that emerge per degree-day > 10°C) are primary phenotypic plant traits that have significant positive direct effects on yield-related traits. Degree-days to heading (DDTH), which has a significant positive effect on grain yield, is influenced by the interaction between MCPNN and MNPR.