Automated single-cell analysis by single-cell side-orifice flow cytometry chain electrospray ionization mass spectrometry.

Single-cell mass spectrometry (SCMS) provides a powerful tool for probing cellular heterogeneity and uncovering biological mechanisms that are often obscured at the population level. However, SCMS faces challenges such as short signal duration and limited tandem mass spectrometry (MS/MS) data for metabolite identification, primarily due to the extremely low sample volume. Previously, we developed the chain electrospray ionization for ultra-low-volume sample analysis.

Characterization of lytic bacteriophage vB_EhoP_ZX13 and its therapeutic potential against Enterobacter hormaechei infection.

Enterobacter hormaechei (E. hormaechei) is an opportunistic pathogen that can cause various animal diseases under certain conditions, including pneumonia, peritonitis, and pyogenic endometritis. The emergence of multidrug-resistant E.

Liposomal Emodin: A nanomedicine for two-photon imaging and mitochondria-targeted photodynamic therapy of psoriasis.

Psoriasis is a chronic, immune-mediated inflammatory skin disorder that severely affects patients' life quality due to its persistent nature, high recurrence rate, and limited treatment efficacy. In this study, we develop liposomal emodin (LE) and systematically evaluate its potential for psoriasis diagnosis and therapeutic treatment, particularly through two-photon-excited photodynamic therapy (TPE-PDT). LE demonstrates remarkable transdermal absorption, effectively penetrating psoriatic lesions and significantly alleviating skin inflammation and hyperkeratosis in an imiquimod-induced psoriasis mouse model.

Proteolysis-targeting chimera (PROTAC) nanomedicines toward cancer treatment: From synthesis to therapeutic delivery.

Proteolysis-targeting chimera (PROTAC) has emerged as a groundbreaking therapeutic strategy by hijacking the endogenous ubiquitin proteasome system (UPS) for targeted protein degradation. These heterobifunctional molecules recruit E3 ligases to recognize the protein of interest (POI) and facilitate its ubiquitination, leading to subsequent proteasomal degradation. Compared to conventional protein inhibitors, PROTACs offer a broader range of target degradation and remain effective even against proteins with drug-resistant mutations.

A Spatiotemporally Controllable DNA Hydrogel Mesh for Focused Antimetastasis Therapy of Cancer.

Cancer is one of the most commonly diagnosed diseases with high mortality, and approximately 50% of patients are prone to present metastasis after various treatments. The shedding of circulating tumor cells (CTCs) during tumor therapy is the root cause of metastasis. In this work, we proposed a focused antimetastasis therapy strategy based on a spatiotemporally controllable DNA hydrogel mesh (SNARE: just like a turtle trapped in the jar) in vivo.

Steel surface defect segmentation with SME-DeeplabV3.

Accurate segmentation of steel surface defects is crucial for ensuring steel quality. This paper presents a steel surface defect segmentation method based on SME-DeepLabV3+ to improve the accuracy and efficiency of segmentation. First, StarNet is adopted as the backbone network, whose unique star operation can achieve efficient transformation from low-dimensional space to high-dimensional features, enhancing the model's ability to capture steel defect features and accurately distinguish between normal and defective areas.

Advancing the modified face name associative memory exam in cognitive aging research: insights into connectomic correlates and task reliability.

Introduction: The shift toward earlier detection in the Alzheimer's disease (AD) continuum underscores the need for more sensitive cognitive outcome assessments (COAs). Traditional COAs may lack precision in capturing cognitive dysfunction during preclinical stages. The Face-Name Associative Memory Exam (FNAME), a cross-modal task that integrates verbal and non-verbal memory, offers enhanced sensitivity and has shown associations with amyloid-β burden across the AD continuum, even in asymptomatic older adults.

Adaptive Optics and Multimodal Retinal Imaging for Postoperative Evaluation in Patients With Epiretinal Membrane.

Purpose: To evaluate adaptive optics scanning laser ophthalmoscope (AO-SLO) features and identify their multimodal retinal imaging characteristics using AO-SLO, swept-source optical coherence tomography (SS-OCT), and color fundus photography in patients undergoing surgery for epiretinal membrane (ERM), and to provide valuable insights into the mechanism underlying postoperative ERM progression.

Methods: Patients with ERM underwent multimodal retinal imaging following surgery, including color fundus photography, SS-OCT, and AO-SLO. AO-SLO features were characterized, and their corresponding multimodal retinal imaging findings were analyzed.

Pirin knockdown alleviates the progression of metabolic dysfunction-associated steatotic liver disease by inhibiting hepatic lipid deposition, inflammation, and fibrosis.

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a global disease with a broad spectrum of symptoms. Pirin (PIR) is involved in a variety of biological and molecular processes. However, the role of PIR in MASLD remains unclear.

Comprehensive genetic analyses of Arabidopsis autophagy-related 8 family reveal redundant regulatory roles during autophagy.

The ubiquitin-like protein ATG8 is a central component of the autophagy process and is required at multiple steps during both bulk and selective autophagy. Currently, our understanding of the roles of ATG8 in plants and the possible functional specialization of its family members is limited by genetic redundancy. Here, we employed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas)9 targeting technology to systematically inactivate all nine Arabidopsis thaliana ATG8 loci.

The glymphatic system as a therapeutic target: TMS-induced modulation in older adults.

While repetitive transcranial magnetic stimulation (rTMS) is a promising neuromodulatory intervention for cognitive impairment, its effects on the glymphatic system remain unexplored in clinical populations. Deficient glymphatic clearance has emerged as a central feature of neurodegenerative disease, which can now be assessed with specialized diffusion magnetic resonance imaging techniques. This study examines changes in the diffusion tensor imaging analysis along the perivascular space (DTI-ALPS) index following theta-burst stimulation (TBS) in older adults with mild cognitive impairment (MCI).

Solar-activated ZnS@MXene heterostructure for integrated radioactive wastewater treatment and energy harvesting.

The presence of ubiquitous organic contaminants in radioactive wastewater poses formidable challenges for conventional uranium extraction technologies. Herein, we architect an auto-photopotential driven catalytic system (APDCS) through rational integration of ZnS@MXene/CF cathodes with monolithic photoanodes, achieving triple-functional uranium recovery, organic pollutant degradation, and simultaneous power generation from complex radioactive effluents. The ZnS@MXene heterostructure, synthesized via ZIF-8 sulfidation on MXene nanosheets, achieved 22-fold faster U(VI) extraction, 4.

Boron-doped-diamond coupling with gold nanoparticles to prepare a simple and highly rigid aptasensor for detection of bleomycin.

Background: Bleomycin (BLM), an essential antitumour medication in clinical applications, has detrimental effects on human health when it appears in an ecological environment, even at trace concentrations. It is desirable to detect trace amounts of BLM by a fast, simple, and efficient method, however, the traditional technologies (e.g.

Influence of Immune Cells on Corneal Nerve Morphological Analysis and Clinical Relevance in Diabetes-Related Dry Eye.

Purpose: Corneal nerve morphology and immune cells are critical biomarkers in the ocular surface. This study aimed to investigate the influence of immune cells on corneal nerve morphology and the clinical significance in diabetes-related dry eye disease (DED).

Methods: In the first part, 1075 in vivo confocal microscopy images containing dendritic cells or round cells were included as system validation.

Artificial intelligence-assisted radiation imaging pathways for distinguishing uterine fibroids and malignant lesions in patients presenting with cancer pain: a literature review.

Uterine fibroids (leiomyomas) are the most common benign uterine tumours, affecting a significant portion of women, and often present with symptoms similar to malignant tumours, such as leiomyosarcoma or endometrial carcinoma, particularly in patients with cancer-related pelvic pain. Conventional imaging modalities, including ultrasound, CT, and MRI, struggle to differentiate between these benign and malignant conditions, often leading to misdiagnoses with potentially severe consequences, such as unnecessary hysterectomies or inadequate treatment for malignancy. Recent advances in artificial intelligence (AI) have begun to address these challenges by enhancing diagnostic accuracy and workflow efficiency.

Immune defense and immune evasion in Mycobacterium tuberculosis Infection: Inspirations and challenges for host directed therapy.

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a major global public health issue, despite improvements in socioeconomic conditions and widespread use of antibiotics. Host immune defense against Mtb infection involve various cells like macrophages, dendritic cells, natural killer cells and T cell subsets, which play distinct roles. By inhibiting phagosome maturation, modulating reactive oxygen and nitrogen species production, regulating host cell death pathway, as well as suppressing antigen presentation and T cell immune responses, the immune escape help Mtb to survive and replicate in macrophages, which ultimately contributes to the development of latent or active TB.

The tumor microbiome in cancer progression: mechanisms and therapeutic potential.

The tumor microbiome (TM) comprises diverse microbial communities, such as bacteria, fungi, and viruses. Recent advancements in microbial sequencing technologies have improved our understanding of the distribution and functional roles of microbes in solid tumors. The TM is formed through several mechanisms, such as direct invasion of mucosal barriers, diffusion from adjacent normal tissues, metastasis of tumor cells, and dissemination via blood and lymphatic circulation.

Size-adjustable nanoparticles co-target macrophages and endothelial cells for enhanced atherosclerosis therapy.

In the pathological process of atherosclerosis (AS), macrophages and dysfunctional endothelial cells form an inflammation-injury axis through inflammatory amplification, oxidative stress, and interaction of signaling pathways. The synergistic targeted intervention of the two cells is the key to improve the plaque microenvironment and achieve disease reversal. However, the lipid core under the vascular intima hinders the penetration of large-sized nanoparticles into dysfunctional macrophages in the plaque, making it hard to block the vicious circle between macrophages and endothelial cells.

Pharmacokinetics, Pharmacodynamics, Safety, and Tolerability of HSK39297 in Chinese Healthy Subjects: A Phase 1 Clinical Trial.

HSK39297 is a novel complement factor B inhibitor, and this phase 1 trial was designed to assess its pharmacokinetics (PK), pharmacodynamics (PD), safety, and tolerability in healthy Chinese subjects. This study included 46 subjects in the single ascending dose (SAD) part (50-600 mg) and 50 subjects in the multiple ascending dose (MAD) part (50-125 mg BID and 200-300 mg QD). Among them, a food effect study was conducted in the SAD 200 mg group.

Bayesian network analysis of individual-level factors associated with bullying among high school students.

Background: This study endeavors to establish a comprehensive network elucidating the multifaceted factors associated with bullying among high school students, elucidating their intricate interrelationships. We aim to develop a theoretical framework that informs the design of targeted and efficacious interventions to mitigate bullying behaviors.

Methods: A cross-sectional survey with a structured questionnaire was administered to 1,401 high school students in Yunnan Province to assess their levels of stress, core self-evaluations, dual mode of self-control, depressive symptoms, and bullying experiences.

Strain-Induced Robust Skyrmion Lattice at Room Temperature in van der Waals Ferromagnet.

Manipulating topological magnetic orders of 2D magnets by strain, once achieved, offers enormous potential for future low-power flexible spintronic applications. In this work, by placing FeGaTe (FGaT), a room-temperature 2D ferromagnet, on flexible substrate, a field-free and robust formation of skyrmion lattice induced by strain is demonstrated. By applying a minimal strain of ≈0.

MXene and PAN-Based Carbon Fiber Enhanced Bimodal Triboelectric Sensor for Robotic Arm Perception and Control.

Endowing robots with human-like perception and thinking to match the growing intelligentization remains a challenge. Here, an MXene and polyacrylonitrile (PAN) based carbon fiber enhanced bimodal triboelectric sensor (MPBS) is proposed to integrate with a commercial robotic arm, establishing a novel paradigm for perception and control. The touchless and tactile perception performance are further improved by a functional layer doped with MXene nanosheets and electrodes composed of PAN-based carbon fibers.

Polypeptide-protein conjugation: A new paradigm for therapeutic protein delivery.

Protein therapeutics have emerged as a potent and highly targeted treatment for a wide range of diseases, including cancer, immune disorders, and genetic conditions. However, their clinical applications are often constrained by challenges such as low stability, short circulation half-life, immunogenic responses, and high manufacturing expenses. The rapid development of biodegradable and biocompatible polypeptide materials provides innovative strategies to address these limitations.

Hsp90 C-terminal domain inhibition enhances ferroptosis by disrupting GPX4-VDAC1 interaction to increase HMOX1 release from oligomerized VDAC1 channels.

Hepatocellular carcinoma (HCC) is one of the most common and lethal malignancies worldwide. Given the critical role of liver in iron storage and metabolism, ferroptosis, characterized by iron-dependent lipid peroxidation and oxidative damage, has become a potential therapy for HCC. Recent research indicated that Voltage-dependent anion-selective channel protein 1 (VDAC1), a key gatekeeper on the outer mitochondrial membrane (OMM), promotes ferroptosis in its oligomeric form.

Comparison of model-based and speckle tracking-based automated three-dimensional echocardiographic quantification of left ventricular volumes and function: A multicenter study.

Background: Three-dimensional echocardiography (3DE) provides accurate and reproducible measurements of left ventricular (LV) volumes and ejection fraction (LVEF). Speckle tracking-based 4D LV-Analysis and model-based Dynamic Heart Model (DHM) are commonly used automated 3DE software. However, the potential discrepancies in quantification between these two software have not been fully elucidated.