Effects of dapagliflozin on podocyte damage and oxidative stress in patients with diabetic nephropathy.

Objective: This study explores the effect of dapagliflozin treatment on podocyte injury and oxidative stress in patients with early diabetic nephropathy (T2DN). Methods: Clinical data from 78 patients with early T2DN, admitted to our hospital between June 2021 and June 2023, were collected and analyzed.

Methods: This study was a single-center, prospective randomized controlled trial.

Emotional Suppression Characteristics and Influencing Factors of Ovarian Cancer Patients - Latent Profile Analysis.

Background: This quantitative study aimed to analyze the potential categories of emotional suppression characteristics and explore the influencing factors of different categories among ovarian cancer chemotherapy patients in Western Region of China.

Methods: From September 2024 to March 2025, a convenience sampling method was used to administer a questionnaire to ovarian cancer chemotherapy patients at a gynecology center within a university hospital situated in the Northwest region of China. The investigation employed general information questionnaires, the Emotional Inhibition Scale (EIS), Social Constraint Scales (SCS), Family APGAR Index (APGAR), and the Hospital Anxiety and Depression Scale (HADS).

Bacteriophage facilitated transmission of multidrug efflux pump regulatory genes in Pseudomonas aeruginosa.

The emergence of multidrug-resistant Pseudomonas aeruginosa strains, primarily driven by efflux pumps that expel antibiotics, poses a serious global health threat. Phages, particularly members of the class Caudoviricetes (94.7 %), play a significant role in the horizontal transfer of genetic material among bacterial hosts, potentially contributing to the acquisition and spread of antibiotic resistance genes.

Enzyme activated fluorescent probe for Vanin-1 multi-dimensional Imaging: Advanced insights for diagnosis and treatment of inflammatory bowel disease.

Vanin-1, a GPI-anchored pantetheinase, has been implicated in various physiological and pathological processes and is a drug target for inflammatory bowel disease (IBD). We developed PHPO, a novel, specific and sensitive fluorogenic biosensor for Vanin-1 and validated its selectivity in VNN1 mice. Using PHPO, we imaged the endogenous distribution of Vanin-1 in intestinal regions, detected expression changes during organoid differentiation, and visually revealed the activation process of Vanin-1 in the colon under the IBD model, further highlighting its pathological role.

Bioactive oligopeptides and the application in skin regeneration and rejuvenation.

Oligopeptides, composed of 2-10 amino acid residues, are protein fragments with unique structural characteristics, including small molecular size, high biocompatibility, and modifiable functional groups. These features endow oligopeptides with excellent permeability, safety, and versatile biological activities, making them widely applicable in disease treatment, drug delivery, and skincare. In particular, oligopeptides have emerged as advanced ingredients in skincare, offering anti-aging, anti-wrinkle, and whitening effects by regulating key biological processes such as collagen synthesis, antioxidant defense, and melanin production.

Readiness for Discharge, Quality of Discharge Teaching, Anxiety and Depression in Surgical Patients With Cervical Cancer: A Cross-Sectional Survey.

Purpose: This quantitative study aimed to determine whether the quality of discharge teaching, anxiety, depression, and various demographic and disease-related factors predict discharge readiness among cervical cancer surgical patients in Western Region of China.

Methods: From November 2023 to May 2024, a convenience sampling method was employed to administer a questionnaire to cervical cancer surgery patients at a tertiary Grade A specialized hospital in Xinjiang. The survey included a patient general information questionnaire, the Quality of Discharge Teaching Scale (QDTS), the Generalized Anxiety Disorder 7-item Scale (GAD-7), a questionnaire assessing the readiness for discharge of gynecological malignant tumor surgery patients under the enhanced recovery after surgery (ERAS) model, and the Patient Health Questionnaire-9 (PHQ-9).

CPK10 protein kinase regulates Arabidopsis tolerance to boron deficiency through phosphorylation and activation of BOR1 transporter.

Boron (B) is crucial for plant growth and development. B deficiency can impair numerous physiological and metabolic processes, particularly in root development and pollen germination, seriously impeding crop growth and yield. However, the molecular mechanism underlying boron signal perception and signal transduction is rather limited.

Intravenous tranexamic acid for intracerebral meningioma resections: A randomized, parallel-group, non-inferiority trial.

Study Objectives: Tranexamic acid (TXA) is an antifibrinolytic that is widely used to reduce surgical bleeding. However, TXA occasionally causes seizures and the risk might be especially great after neurosurgery. We therefore tested the hypothesis that TXA does not meaningfully increase the risk of postoperative seizures within 7 days after intracranial tumor resections.

The role of cell death in SARS-CoV-2 infection.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), showing high infectiousness, resulted in an ongoing pandemic termed coronavirus disease 2019 (COVID-19). COVID-19 cases often experience acute respiratory distress syndrome, which has caused millions of deaths. Apart from triggering inflammatory and immune responses, many viral infections can cause programmed cell death in infected cells.

Arsenic exposure caused male infertility indicated by testis and sperm metabolic dysfunction in SD rats.

Arsenic containment is one of the most severe environmental problems. It has been reported that arsenic exposure could cause male reproductive damage. However, the evidence chain from sodium arsenite (NaAsO) exposure to adverse male fertility outcomes has not been completed by molecular events.

The effect of tranexamic acid on intraoperative blood loss in patients undergoing brain meningioma resections: Study protocol for a randomized controlled trial.

Introduction: Tranexamic acid (TXA) has been proven to prevent thrombolysis and reduce bleeding and blood transfusion requirements in various surgical settings. However, the optimal dose of TXA that effectively reduce intraoperative bleeding and blood product infusion in patients undergoing neurosurgical resection of meningioma with a diameter ≥ 5 cm remains unclear.

Methods: This is a single-center, randomized, double-blinded, paralleled-group controlled trial.

Ozone exposure associates with sperm quality indicators: Sperm telomere length as a potential mediating factor.

Evidence linking O exposure and human semen quality is limited and conflicting and the mechanism underlying the association remains unclear. Therefore, we investigated the associations between ambient O exposure and sperm quality parameters and explored the mediating role of sperm mitochondrial DNA copy number (mtDNAcn) and sperm telomere length (STL) among 1068 potential sperm donors who provided 5002 repeated semen samples over approximately 90 days. We found that every 10 μg/m increase in O exposure was associated with a decrease in STL, sperm concentration, total count, total motile sperm number, and semen volume.

Progress of cGAS-STING signaling in response to SARS-CoV-2 infection.

Coronavirus disease 2019 (COVID-19) is an epidemic respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that can cause infections in millions of individuals, who can develop lung injury, organ failure, and subsequent death. As the first line of host defense, the innate immune system is involved in initiating the immune response to SARS-CoV-2 infection and the hyperinflammatory phenotype of COVID-19. However, the interplay between SARS-CoV-2 and host innate immunity is not yet well understood.

Cloning and Functional Identification of Gibberellin Receptor SvGID1s Gene of Salix viminalis.

Gibberellins (GAs) play a key role in the transition from vegetative growth to flowering and the GA receptor GID1 (GIBBERELLIN INSENSITIVE DWARF1) is the central part of GA-signaling. The differential expression of SvGID1 was found in the transcriptome sequencing in our previous study, which was further verified at different stages of flowering of Salix viminalis. In order to reveal the function GID1 of S.

Entrapment of cyanidin-3-O-glucoside in β-conglycinin: From interaction to bioaccessibility and antioxidant activity under thermal treatment.

The thermal-induced interaction between β-conglycinin (7S) and cyanidin-3-O-glucoside (C3G) on the bioaccessibility and antioxidant capacity of C3G was investigated. High ratio of 7S to C3G (1:100) led to a more ordered secondary structure of 7S. Thermal treatment promoted the formation of 7S-C3G complexes via hydrophobic and hydrogen bonds but did not induce the formation of 7S-C3G covalent products.

The role of cyclophilins in viral infection and the immune response.

Cyclophilins (Cyps) are a subgroup of peptidyl-prolyl cis-trans isomerases (PPIases) that contain a highly conserved domain of PPIases. Sixteen Cyps have been identified in humans, among which the functions of five classical Cyp subtypes (CypA, B, C, D, and 40) have been studied in more detail. Cyps are widely expressed in almost all human tissues and are involved in several intracellular signaling pathways such as oxidative stress, mitochondrial dysfunction, cell migration, and apoptosis.

MST4 negatively regulates type I interferons production via targeting MAVS-mediated pathway.

Background: Cytosolic RNA sensing can elicit immune responses against viral pathogens. However, antiviral responses must be tightly regulated to avoid the uncontrolled production of type I interferons (IFN) that might have deleterious effects on the host. Upon bacterial infection, the germinal center kinase MST4 can directly phosphorylate the adaptor TRAF6 to limit the inflammatory responses, thereby avoiding the damage caused by excessive immune activation.

Environmental metal exposure, seminal plasma metabolome and semen quality: Evidence from Chinese reproductive-aged men.

Environmental metal exposure has been associated with decreasing semen quality, but the effects of multiple metal exposure on seminal plasma metabolome remain obscure. In this study, semen and repeated urine samples from 551 volunteers were collected in Wuhan City. Heavy metals and trace elements were measured using inductively coupled plasma mass spectrometer, and seminal plasma metabolomes were acquired using liquid chromatography coupled with high-resolution mass spectrometry.

Identification of core genes associated with type 2 diabetes mellitus and gastric cancer by bioinformatics analysis.

Background: Gastric cancer (GC) is the most common type of malignant neoplasm of the digestive system. Diabetes mellitus (DM) or hyperglycemia may increase the incidence or mortality of GC. We aimed to investigate the possible genetic relationship between GC, DM, and type 2 diabetes mellitus (T2DM), and to identify core genes that are associated with T2DM and GC.

Efficacy evaluation of Lattice Carbon Dioxide Laser Therapy in the treatment of postmenopausal patients with mild to moderate stress urinary incontinence.

Objectives: To investigate the efficacy and postoperative complications of lattice carbon dioxide laser in the treatment of postmenopausal patients with mild to moderate stress urinary incontinence.

Methods: A total of 30 postmenopausal female patients with mild to moderate stress urinary incontinence, recruited from the Affiliated Hospital of Hebei University from September to November 2019, were selected as the study subjects and treated with lattice carbon dioxide laser therapy. Treatment was given at intervals of one month.

Cyclophilin A regulates the apoptosis of A549 cells by stabilizing Twist1 protein.

Cyclophilin A (CypA, also known as PPIA) is an essential member of the immunophilin family. As an intracellular target of the immunosuppressive drug cyclosporin A (CsA) or a peptidyl-prolyl cis/trans isomerase (PPIase), it catalyzes the cis-trans isomerization of proline amidic peptide bonds, through which it regulates a variety of biological processes, such as intracellular signaling, transcription and apoptosis. In this study, we found that intracellular CypA enhanced Twist1 phosphorylation at Ser68 and inhibited apoptosis in A549 cells.

Berberine attenuates atherosclerotic lesions and hepatic steatosis in ApoE mice by down-regulating PCSK9 via ERK1/2 pathway.

Background: It has been demonstrated that berberine (BBR), a kind of alkaloid derived from Chinese herbal medicine, has multiple pharmacological effects on human's diseases including anti-atherosclerosis action. However, although the previous studies showed that the beneficial impact of BBR on atherosclerosis might be associated with proprotein convertase subtilisin/kexin type 9 (PCSK9), the exact underlying mechanism are not fully determined. The present study aimed to investigate potential mechanisms of anti-atherosclerosis by BBR using ApoE mice.

SARS-CoV-2 3C-like protease antagonizes interferon-beta production by facilitating the degradation of IRF3.

The prevalence of SARS-CoV-2 is a great threat to global public health. However, the relationship between the viral pathogen SARS-CoV-2 and host innate immunity has not yet been well studied. The genome of SARS-CoV-2 encodes a viral protease called 3C-like protease.

The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.

In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×10  Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated. The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.

Interaction of Soy Protein Isolate Hydrolysates with Cyanidin-3--Glucoside and Its Effect on the In Vitro Antioxidant Capacity of the Complexes under Neutral Condition.

The interaction of soy protein isolate (SPI) and its hydrolysates (SPIHs) with cyanidin-3--glucoside (C3G) at pH 7.0 were investigated to clarify the changes in the antioxidant capacity of their complexes. The results of intrinsic fluorescence revealed that C3G binds to SPI/SPIHs mainly through hydrophobic interaction, and the binding affinity of SPI was stronger than that of SPIHs.