Effectiveness of Endovascular Treatment for Acute Mild Basilar Artery Occlusion Stroke: A Multicenter Real-World Study.

Background: Randomized trials have demonstrated the benefit of endovascular treatment (EVT) in moderate-to-severe acute basilar artery occlusion (BAO), but its benefit in acute mild BAO remains uncertain. This multicenter, retrospective cohort study aimed to explore the efficacy of EVT in acute BAO with a low National Institutes of Health Stroke Scale.

Methods: Patients with acute BAO and National Institutes of Health Stroke Scale <10, hospitalized at 5 stroke centers in China between January 2020 and December 2023, were included and divided into EVT and standard medical treatment groups.

Flexible Hydrogen-Bonded Organic Framework with Anthracene Core for Recognition of Benzene Over Cyclohexane.

Flexibility is considered one of the most critical features of framework materials, playing a vital role in applications such as adsorption and separation. Hydrogen-bonded organic frameworks (HOFs) exhibit unique flexibility derived from their weak, reversible hydrogen-bonding interactions; however, realizing controlled and functional flexible behavior in HOFs remains a synthetic challenge. Herein, we report a flexible hydrogen-bonded organic framework, HOF-FJU-119, constructed from an anthracene-core, that exhibits multimode guest-responsive behavior.

Multi-source biochar: Effects on composting humification, soil properties and plant growth.

Amid rising organic waste pressures, biochar derived from thermochemical conversion of biomass has emerged as a promising tool for enhancing compost quality and promoting sustainable soil management. This study evaluated five biochars derived from kitchen waste, pig manure, sewage sludge, distillers' grains, and biogas residue for their effects on compost performance, humification, and the soil-plant system. Composting and pot experiments were conducted to evaluate humification, soil nutrient availability, and plant responses.

Efficacy and safety of transcutaneous auricular vagus nerve stimulation plus pregabalin for radiotherapy-related neuropathic pain in patients with head and neck cancer (RELAX): a phase 2 randomised trial.

Background: Rapid relief of radiotherapy-related neuropathic pain (RRNP) in head and neck cancer (HNC) survivors is challenging when relying solely on pharmacologic treatments, especially in the early stages of medication. Whether transcutaneous auricular vagus nerve stimulation (taVNS) can effectively and rapidly control the RRNP in HNC survivors remains uncertain.

Methods: The RELAX trial was a randomised, parallel, sham-controlled trial conducted at four centres in China.

Intratumoral amino acid insufficiency limits CD8 T-cell effector function.

Loss of effector function is a hallmark of tumor-infiltrating CD8 T-cells that have lost therapeutic efficacy. This impaired capacity occurs despite expression of transcripts encoding cytotoxic proteins, raising the possibility that post-transcriptional suppression of cytotoxic protein synthesis limits anti-tumor immunity. Whether altered protein synthesis contributes to CD8 T-cell dysfunction has not been explored.

Landscape of mobile genetic elements and their functional cargo across the gastrointestinal tract microbiomes in ruminants.

Background: Mobile genetic elements (MGEs) drive horizontal gene transfer and microbial evolution, spreading adaptive genes across microbial communities. While extensively studied in other ecosystems, the role of MGEs in shaping ruminant gastrointestinal microbiomes-especially their impact on diversity, adaptation, and dietary responsiveness-remains largely unexplored. This study systematically profiles MGE distribution and functionality across gastrointestinal regions in multiple ruminant species to advance our understanding of microbial adaptation.

Continuous cell lines from kidney and spleen of Japanese eel, Anguilla japonica, with capacity for antiviral immune response.

In this study, two stable cell lines were established from the kidney (AJK) and spleen (AJSP) tissues of Japanese eel (Anguilla japonica) using the tissue explant method, with the aim of supporting immunological research in this species. Following isolation, AJK and AJSP cells were cultured in DMEM/F-12 medium supplemented with 20 % fetal bovine serum (FBS) at 28 °C, with passaging every 3-4 days. After the fifth passage, the cells were maintained in DMEM/F-12 containing 15 % FBS for passage culture.

Cognitive Decline in Nasopharyngeal Carcinoma Survivors with Post-Radiation Epilepsy: A Prospective Cohort Study.

Cognitive decline is a major concern for nasopharyngeal carcinoma (NPC) survivors after radiotherapy (RT). We assessed whether the rates of cognitive decline in NPC survivors differed depending on the presence of epilepsy. Based on an ongoing prospective cohort study (NCT03908502), we included consecutive NPC patients with a history of radiotherapy who underwent a baseline and follow-up cognition assessment between January 2005 and December 2023.

APOE4 triggers dysregulated synaptic vesicle release by disrupting SNARE complex assembly.

The ε4 allele of the Apolipoprotein E (APOE) gene is an important genetic risk factor for several neurodegenerative diseases, while the common pathogenic mechanism is still unclear. Impaired synaptic transmission is one of the common pathogenic features of neurodegenerative diseases. By using proteomics analysis, co-immunoprecipitation (Co-IP), and bimolecular fluorescence complementation (BiFC) assay, we demonstrated that APOE interacts with VAMP2, a core component of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, in an APOE4 > APOE3 manner.

Metabolic network remodeling through PxJHE modulates temperature adaptation in a cosmopolitan insect.

Understanding the molecular mechanisms underlying temperature adaptation in agricultural pests is crucial for predicting their evolutionary responses to climate change. Here, we investigate the role of juvenile hormone esterase gene PxJHE in temperature adaptation of Plutella xylostella, a globally distributed pest. Spatial-temporal expression patterns demonstrates significantly reduced PxJHE transcript levels in hot-evolved (HS), and cold-evolved (CS) strains across all tested temperatures compared to ancestral strain (AS).

Nano-orchestrated magnetotactic-like navigation for electromagnetic theranostics and immune enhancement via photoautotrophic oxygenation, mild hyperthermia, and ferroptosis.

Overcoming the hypoxic tumor microenvironment (TME) and immune suppression remains a significant challenge in solid bladder tumor therapies. This study introduces a translational system of nano-orchestrated magnetotactic-like system, integrating photosynthetic oxygenation, remote hyperthermia, and ferroptosis to achieve comprehensive tumor eradication and immune activation. The developed system, composed of electromagnetic-responsive iron oxide nanoparticles (IO NPs) encapsulated within a glycol chitosan (GCS) matrix and coated onto Chlorella (CHL; CHL-GCS-IO NPs), exhibited versatility for precise magnetic targeting, photothermal-hypertehrmia and photosynthesis-driven oxygen generation under light irradiation.

Prevalence and factors associated with Chinese herbal medicine use among middle-aged and older Chinese adults with diabetes mellitus.

Background: The effectiveness of traditional Chinese medicine (TCM) in treating diabetes has been confirmed in China and globally. However, research on the use of Chinese herbal medicine (CHM) among middle-aged and elderly patients with diabetes and its associated factors is limited. This study aims to explore the prevalence of CHM use among these patients and its associated factors.

Edible polyethylene glycol modified chitosan nanoparticles: Separation and controlled release of tea polyphenols from multiphasic systems.

Tea polyphenols have potent antioxidant and lipid-regulating properties but suffer from low bioavailability and instability. Here, we present a novel PEG (polyethylene glycol)-modified chitosan nanoparticle system to adsorb and separate tea polyphenols from tea extract. It incorporates a hydrophilic PEG layer to enhance stability and adsorption efficiency compared to traditional chitosan nanoparticles.

Asymmetric heterogeneous catalysis using crystalline porous materials.

Asymmetric catalysis has emerged as a pivotal strategy in the synthesis of chiral compounds, offering significant advantages in selectivity and efficiency. In recent years, heterogeneous catalysis has become a focal point in the fields of organic synthesis and materials science due to continuous advancements in science and technology, especially the use of crystalline porous materials (CPMs) as catalysts. This review summarizes recent advances in using CPMs, such as metal-organic frameworks (MOFs), covalent organic frameworks (COFs) and zeolites, as promising supports for asymmetric catalysts.

Analysis of the association between long-term exposure to low-dose ionizing radiation and dyslipidemia and its components in medical radiologists: The mediating role of inflammatory markers.

Introduction: Our study aimed to explore the association between long-term exposure to low-dose ionizing radiation (LDIR) and dyslipidemia and its components among medical radiologists, and to identify the mediating role of inflammatory markers.

Methods: This cross-sectional study was conducted on 3918 medical radiologists, with data collected through questionnaires and occupational external exposure dosimeters. The multifactorial logistic regression and restricted cubic spline model were used to analyze the association between long-term exposure to LDIR and dyslipidemia and its components among medical radiologists, and mediation analysis was used to identify potential mediation effects.

Nomogram for Predicting Hemorrhagic Transformation Risk in Acute Ischemic Stroke Patients With Atrial Fibrillation.

Background: Hemorrhagic transformation (HT) is a critical complication in acute ischemic stroke (AIS) patients with atrial fibrillation (AF) awaiting anticoagulation reinitiation. No reliable predictive model exists for assessing HT risk for these patients. Clinical decisions typically rely on NIHSS score and infarct size; however, other relevant risk factors remain insufficiently explored.

MeCP2 Lactylation Protects against Ischemic Brain Injury by Transcriptionally Regulating Neuronal Apoptosis.

Lactate plays diverse roles in brain pathophysiology, including ischemic stroke. Here, the role of lysine lactylation, an epigenetic modification of lactate, in cerebral ischemia is investigated. Using a mouse model of transient middle cerebral artery occlusion, increased brain lactate levels and global protein lactylation are observed.

Effects of maternal blood beta-hydroxybutyrate on brown adipose tissue functions and thermogenic and metabolic health in neonatal calves.

Maternal metabolic health, particularly during late pregnancy, plays a crucial role in fetal development and postnatal metabolic function. Elevated levels of BHB in dry cows, commonly observed in late gestation, may affect offspring development, but the effects on brown adipose tissue (BAT) and metabolic health remain unclear. In this study, 60 pregnant Holstein dairy cows were categorized into 2 groups based on serum BHB concentrations measured at 1, 3, 5, and 7 wk after dry-off: maternal low BHB (n = 30; mean ± SEM, 0.

Balancing LncRNA H19 and miR-675 Bioconversion as a Key Regulator of Embryonic Myogenesis Under Maternal Obesity.

Background: Maternal obesity (MO) impairs fetal skeletal muscle development, but the underlying mechanisms remain poorly defined. The regulatory roles of lncRNA H19 and its first exon derived microRNA675 (miR675) in prenatal muscle development remain to be examined. H19/Igf2 are in the same imprinting cluster with H19 expressed from the maternal allele while Igf2 expresses paternally.

High Proton Conductivity Enhancement Obtained by a Covalent Postsynthesis Modification Approach for Two Metal-Organic Frameworks.

This study demonstrates an effective strategy to enhance proton conductivity by synthesizing 2 three-dimensional metal-organic frameworks (MOFs), [Zn(DTD22)] (MOF 1) and [Cd(DTD22)] (MOF 2), (DTD22 = 4,4″-diamino-[1,1':4',1″-terphenyl]-2,2″-dicarboxylic acid). The DTD22 ligand used formed a continuous hydrogen-bonding network in the structure, constructing excellent hydrophilic channels. MOF 1 and MOF 2 were further postsynthesized and modified (PSM) by Schiff base reaction, and 4-chloro-3-formylbenzenesulfonic acid ligands containing -SOH and -Cl were successfully introduced into the framework to form PSM-MOF 1 and PSM-MOF 2.

OncomiR mdv1-miR-M7-5p promotes avian lymphomatosis by modulating the BCL2/Bax mitochondrial apoptosis signaling pathway.

Marek's disease virus (MDV) is an oncogenic alphaherpesvirus that infects poultry and causes fatal lymphomas in infected chickens. Notably, the mdv1-miR-M7-5p, a pivotal oncomiR encoded by MDV, is closely associated with viral replication and latency. Here, mdv1-miR-M7-5p was transfected into the chicken lymphoma cell line MSB1, which resulted in the inhibition of lymphoma cell apoptosis and an increase in lymphoma cell proliferation and migration.

Targeting allograft inflammatory factor 1 reprograms kidney macrophages to enhance repair.

The role of macrophages (MΦs) remains incompletely understood in kidney injury and repair. The plasticity of MΦs offers an opportunity to polarize them toward mediating injury resolution in both native and transplanted kidneys undergoing ischemia and/or rejection. Here, we show that infiltrating kidney MΦs augmented their own allograft inflammatory factor 1 (AIF-1) expression after injury.

Promoting Photocatalytic Hydrogen Evolution by Stabilization of Excited Triplet States and Enhancement of Internal Electric Field at Dye/PCN Interface.

The heterojunction photocatalysts composed of organic dyes and polymeric carbon nitride (PCN) have great potential for photocatalytic hydrogen evolution (PHE). However, serious charge recombination exists at the dye/PCN interface for the large gaps in time scales and the poor driving force of charge transfer process. Herein, both the excited triplet states of organic dyes with long lifetimes and strong internal electric fields (IEF) as charge transfer driving forces are achieved by the construction of high dipole moments with aromatic-core engineering, and modulation of dye aggregates by alkyl modification.

Association of immune-mediated diseases with the risk of dementia and brain structure in UK Biobank participants.

Background: Immunity and inflammation may be essential to the pathogenesis of dementia. However, the association of immune-mediated diseases with the risk of incident dementia has not been well characterised.

Objectives: We aimed to investigate the prospective association of 27 immune-mediated diseases and incident dementia risk and to explore the underlying mechanisms driven by brain structures.