Low-liver-accumulation lipid nanoparticles enhance the efficacy and safety of HPV therapeutic tumor vaccines.

Background: Lipid nanoparticles (LNPs) hold significant potential in vaccine and cancer therapy, but conventional LNPs often cause hepatotoxicity and reduced efficacy due to liver accumulation.

Methods: LNP molecules with varying tail lengths were synthesized in vitro. Transfection efficiency was assessed in vitro via flow cytometry.

Alleviating Clostridium perfringens-Induced Gut Lesionsin Broiler Chickens by Orally Administered Bovine Intestinal Alkaline Phosphatase.

Intestinal alkaline phosphatase (AP) detoxifies lipopolysaccharides, which has been exploited in treating infection of Gram-negative bacterial pathogens such as Escherichia coli. Orally administered AP has the potential to modulate the gut microbiota, which is in part ascribed to its ability to degrade ATP, a well-known inhibitor of gut commensal bacteria. In addition, AP can fortify the gut barrier.

Feasibility and effects of high-intensity interval training in older adults with mild to moderate depressive symptoms: A pilot cluster-randomized controlled trial.

Background: Older adults with mild-to-moderate depressive symptoms (MMDS) are at high risk of developing severe depression along with mortality and disability. The aim of this study was to investigate the feasibility and preliminary effects of high-intensity interval training (HIIT) in older adults with MMDS in a small sample size.

Methods: Three elderly centers involving 24 older adults with MMDS were randomized into: 1) HIIT (n = 8); 2) moderate-intensity continuous training (Baduanjin Qigong) (MICT-BDJ) (n = 8); or 3) recreation workshop (RW) control (n = 8) for 16-week (32 session) intervention.

Correction: Stem cell factor restrains endoplasmic reticulum stress-associated apoptosis through c-Kit receptor activation of JAK2/STAT3 axis in hippocampal neuronal cells.

[This corrects the article DOI: 10.1371/journal.pone.

Effects of maternal blood beta-hydroxybutyrate on brown adipose tissue functions and thermogenic and metabolic health in neonatal calves.

Maternal metabolic health, particularly during late pregnancy, plays a crucial role in fetal development and postnatal metabolic function. Elevated levels of BHB in dry cows, commonly observed in late gestation, may affect offspring development, but the effects on brown adipose tissue (BAT) and metabolic health remain unclear. In this study, 60 pregnant Holstein dairy cows were categorized into 2 groups based on serum BHB concentrations measured at 1, 3, 5, and 7 wk after dry-off: maternal low BHB (n = 30; mean ± SEM, 0.

A Novel Hidden Protein p-414aa Encoded by ) Inhibits Vascular Remodeling.

Background: Phenotypic switching of vascular smooth muscle cells (VSMCs), leading to neointimal hyperplasia, is a fundamental cause of vascular remodeling diseases such as atherosclerosis and hypertension. Novel hidden proteins encoded by circular RNAs play crucial roles in disease progression, yet their involvement in vascular remodeling diseases has not been comprehensively studied. This study identifies a novel protein derived from a circular RNA in VSMCs and demonstrates its potential role in regulating vascular remodeling.

Hsa_circ_0001304 promotes vascular neointimal hyperplasia accompanied by autophagy activation.

Aberrant autophagy in vascular smooth muscle cells (VSMCs) is associated with the progression of vascular remodeling diseases caused by neointimal hyperplasia. Platelet-derived growth factor-BB (PDGF-BB)-induced vascular remodeling is accompanied by autophagy activation, however, the involvement of circular RNAs (circRNAs) remains unclear. Here, we show the role of PDGF-BB-regulated hsa_circ_0001304 (circ-1304) in neointimal hyperplasia and its potential involvement in VSMC autophagy, while also elucidating the potential mechanisms.

Alleviating -Induced Intestinal Lesions in Chickens Using the Xylanase Xyn10C and Its Binary Cocktail with a Protease.

infection can induce necrotic enteritis and lead to significant economic loss to the chicken industry. In this study, a xylanase (Xyn10C), which effectively promotes the growth of probiotics, and a protease, which degrades the biofilm of were analyzed for their ability to alleviate -induced necrotic enteritis in broiler chickens. A total of 300 male AA chickens were divided into five treatment groups (control, no enzyme and no challenge; Cp, no enzyme, challenge; Xyn, Xyn10C plus challenge; Xyn+Am, Xyn10C+Amylase plus challenge; Xyn+Ap, Xyn10C+Alkaline protease plus challenge).

Knockdown of the ZcVgR Gene Alters the Expression of Genes Related to Reproduction and Lifespan in Zeugodacus cucurbitae (Coquillett) Under Extreme Heat Conditions.

Zeugodacus cucurbitae (Coquillett) is an important migratory vegetable pest. Previous research has demonstrated that short-term high temperatures induce differential expression of the vitellogenin receptor (ZcVgR) gene, reducing the number of eggs laid and the lifespan of female Z. cucurbitae.

Stem cell factor restrains endoplasmic reticulum stress-associated apoptosis through c-Kit receptor activation of JAK2/STAT3 axis in hippocampal neuronal cells.

Background: Alzheimer's disease (AD) is a common elderly disorder characterized by cognitive decline. Endoplasmic reticulum (ER) stress has been implicated in various neurodegenerative diseases, including AD. Stem cell factor (SCF) performs its biological functions by binding to and activating receptor tyrosine kinase c-Kit.

Synergistic Tribological Effects of TiCT MXene and ZDDP under Simple Grafting Strategies for Efficient Lubrication.

MXenes, a novel class of two-dimensional materials, possess exceptional physical and chemical properties, positioning them as promising candidates for lubricant additives. However, their potential is constrained by challenges in dispersion and stability, coupled with a paucity of research on interactions with additives in full-formula oils. In this study, hexadecylphosphonic acid (HDPA) is grafted onto TiCT to formulate a polyalkylene glycol dispersion system.

Preparation and Photocatalytic Properties of AlO-SiO-TiO Porous Composite Semiconductor Ceramics.

Titanium dioxide (TiO) is widely employed in the catalytic degradation of wastewater, owing to its robust stability, superior photocatalytic efficiency, and cost-effectiveness. Nonetheless, isolating the fine particulate photocatalysts from the solution post-reaction poses a significant challenge in practical photocatalytic processes. Furthermore, these particles have a tendency to agglomerate into larger clusters, which diminishes their stability.

The Gene Regulates the Reproduction and Lifespan of Female (Coquillett) Mediated by Short-Term High Temperatures.

(Coquillett) is a significant pest affecting fruit and vegetables in tropical and subtropical regions, and its development and reproduction are enhanced after exposure to short-term high-temperature stress at 45 °C. Vitellogenin (Vg) is an essential precursor of yolk protein formation in eggs and plays a vital role in the ovarian development of insects. Interfering with the () gene in short-term high-temperature conditions decreases the fecundity of female adults, while the transcription level of the gene increases.

Sub-genomic RNAi-assisted strain evolution of filamentous fungi for enhanced protein production.

Genetic engineering at the genomic scale provides a rapid means to evolve microbes for desirable traits. However, in many filamentous fungi, such trials are daunted by low transformation efficiency. Differentially expressed genes under certain conditions may contain important regulatory factors.

Childhood adversity, perceived social support, and depressive symptoms among pre-abortion Chinese women.

Background: Unintended (unwanted) pregnancy is a sexual and reproductive health issue with psychosocial consequences for the individual, their family, and society. However, the relationship between social support and related mental health issues, like depression and the effects of childhood adversity, is poorly studied. This study aims to explore the connections between childhood adversity, perceived social support, and depressive symptoms in pre-abortion women (women who have decided to have an abortion) in a clinical setting, based on the common risk factor approach and social support theory.

Preparation and indoor virulence determination of a temperature-sensitive insecticide against Zeugodacus cucurbitae (Coquillett).

Zeugodacus cucurbitae (Coquillett) is an important fruit and vegetable pest, especially in high-temperature seasons. In our previous research, we developed a temperature-sensitive sustained-release attractant for Z. cucurbitae, that not only can control the release rate of cuelure according to the temperature change, but also shows an excellent trapping effect on Z.

Longitudinal analysis of quality of life in primary lung cancer patients with chlamydia pneumoniae infection: a time-to-deterioration model.

Background: Chlamydia pneumoniae (Cpn) IgG and IgA has been strongly linked to lung cancer, but its impact on patients' quality of life remains unclear. Our objective was to investigate the relationship between pre-treatment Cpn IgG and IgA and time to deterioration (TTD) of the HRQoL in patients with primary lung cancer.

Methods: A prospective hospital-based study was conducted from June 2017 to December 2018, enrolling 82 patients with primary lung cancer admitted to the First Affiliated Hospital of Fujian Medical University for questionnaire surveys.

is responsible for posterior pharyngeal cartilage formation by inhibiting Fgf signaling.

Nkx2.3, a transcription factor, plays important roles in various developmental processes. However, the mechanisms underlying ' regulation of pouch and pharyngeal arch development in zebrafish remain unclear.

Two novel CHN1 variants identified in Duane retraction syndrome pedigrees disrupt development of ocular motor nerves in zebrafish.

Duane retraction syndrome (DRS) is a rare congenital eye movement disorder causing by the dysplasia of abducens nerve, and has highly variable phenotype. MRI can reveal the endophenotype of DRS. Most DRS cases are sporadical and isolated, while some are familial or accompanied by other ocular disorders and systemic congenital abnormalities.

Hsa_circ_0001402 alleviates vascular neointimal hyperplasia through a miR-183-5p-dependent regulation of vascular smooth muscle cell proliferation, migration, and autophagy.

Introduction: Vascular neointimal hyperplasia, a pathological process observed in cardiovascular diseases such as atherosclerosis and pulmonary hypertension, involves the abundant presence of vascular smooth muscle cells (VSMCs). The proliferation, migration, and autophagy of VSMCs are associated with the development of neointimal lesions. Circular RNAs (circRNAs) play critical roles in regulating VSMC proliferation and migration, thereby participating in neointimal hyperplasia.

Dynamic Contrast-Enhanced MRI Assessing Antifibrotic Therapeutic Effects of Pancreatic Fibrosis with Curcumin - An Experimental Study at 11.7 T.

Rationale And Objectives: Pancreatic fibrosis is the hallmark of chronic pancreatitis (CP), which is associated with microcirculatory disturbance. Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) can assess the perfusion and permeability of the pancreas by providing information about microcirculation. We hypothesize that DCE-MRI parameters can be utilized to assess pancreatic fibrosis and may furthermore provide an opportunity to evaluate response to antifibrotic treatment with curcumin.

The gene is involved in lifespan regulation of (Coquillett) after short-term high-temperature treatment.

(Coquillett) is a highly damaging agricultural pest in many tropical and subtropical countries around the world and high temperatures usually affect its survival. To clarify the effect of short-term high temperatures on the survival and lifespan of , newly emerged adults of three consecutive generations (F, F, and F) were exposed to 25 °C, 33 °C, 37 °C, 41 °C, or 45 °C treatments for 1 h. The effect of these temperatures on survival and lifespan was evaluated using biological indicators such as lifespan and pupation rate.