H3K27ac Is Essential for Human Naive Pluripotency Modulated by m6A-Driven EP300 Expression.

Human pluripotent stem cells (hPSCs) can transition between primed and naive states, each defined by unique epigenetic features crucial for early human development. However, the precise regulatory mechanisms of these transitions, particularly involving the roles of H3K27ac and m6A modifications, remain unclear. Here, it is revealed that H3K27ac is essential for establishment of human naive pluripotency.

3D printing of structural bionic and functionalized hydrogels for the construction of macroscale human cardiac tissues.

Capturing the intricate structural, mechanical, and electrophysiological properties of the native heart in models is crucial for achieving efficient physiological pumping function; however, current approaches have shown limited success in replicating these features essential for producing tissue models on complex geometries that accurately mimic full cardiac function. Here, we present a novel hydrogel ink formulation combining a conductive, biocompatible ionic liquid with a photosensitive poly(vinyl alcohol)-based hydrogel, enabling 3D printing of biomechanically compatible heart valves and 3D tissue engineering scaffolds. These scaffolds mimic the helical and circumferential alignments characteristic of the ventricular and atrial muscle layers, respectively, and incorporate a hollow auxetic structure to achieve mechanical anisotropy.

OsATG1 and OsATG8 exhibit autophagy-independent functions to oppositely regulate ROP GTPase-mediated plant immunity in rice.

ROP GTPases regulate various cellular processes, including plant immunity. While ROP GTPase activation has been reported during plant immune responses, the mechanisms underlying the dynamic deactivation of ROP GTPases remain unclear. In this study, we identified the autophagy kinase OsATG1 as a key regulator that interacts with and phosphorylates SPIN6, a plant-specific ROP GTPase-activating protein (RhoGAP), which in turn deactivates the ROP GTPase OsRac1.

Multi-kingdom microbial changes and their associations with the clinical characteristics in schizophrenia patients.

Accumulating evidence has highlighted alterations in the gut microbiome in schizophrenia (SZ); however, the role of multi-kingdom microbiota in SZ remains inadequately understood. In this study, we performed metagenomic sequencing of fecal samples from 36 SZ patients and 55 healthy controls (HC) to profile bacterial, fungal, archaeal, and viral communities, along with functional pathways. We also conducted co-occurrence network analysis to explore the relationships among differential microbial species and metabolic pathways separately.

Immersion Phase Separation 3-Dimensional Printing for Strain-Stiffening Hydrogel Scaffolds.

Strain-stiffening hydrogels, which mimic the nonlinear mechanical behavior of biological tissues such as skin, arteries, and cartilage, hold transformative potential for biomedical applications. This study introduces immersion phase separation (IPS) 3-dimensional (3D) printing, an innovative technique that enables the one-step fabrication of strain-stiffening hydrogel scaffolds with intricate, hierarchical architectures. This technique addresses the long-standing challenge of balancing structural complexity and intrinsic mechanoresponsive behavior in traditional hydrogel fabrication methods.

Receptor-like cytoplasmic kinases mediated signaling in plant immunity: convergence and divergence.

Receptor-like cytoplasmic kinases (RLCKs) function as a central player in plant receptor kinases-mediated signaling, which regulate various aspects of plant immunity and growth. RLCKs receive signals from pattern recognition receptors (PRRs) to activate pattern-triggered immunity (PTI), including reactive oxygen species (ROS) production, Ca influx, mitogen-activated protein kinase (MAPK) cascades, cellulose synthesis, phosphatidic acid (PA) production, hormone synthesis and signaling, and transcriptional remodeling. Besides, RLCK also participate in effector-triggered immunity (ETI) and the interplay between ETI and PTI.

Bionic-structured electromagnetic interference shielding composites.

As electromagnetic pollution intensifies and the demand for high-precision electronic equipment increases, the development of high-performance, adaptable electromagnetic interference (EMI) shielding materials with high-performance and adaptability has become a research focus. Inspired by natural structures, bionic designs offer unique advantages for the fabrication of advanced EMI shielding materials. These materials provide effective electromagnetic wave attenuation and are lightweight, flexible, and environmentally adaptable, thereby overcoming the limitations of traditional EMI shielding materials.

Toripalimab plus bevacizumab versus sorafenib as first-line treatment for advanced hepatocellular carcinoma (HEPATORCH): a randomised, open-label, phase 3 trial.

Background: Although several PD-1 or PD-L1 inhibitors combined with antiangiogenic agents have been approved as first-line treatment of advanced hepatocellular carcinoma, treatment needs remain unmet given the high incidence and mortality of hepatocellular carcinoma and due to factors such as regional approval status, medical insurance restrictions, and cost considerations. In this phase 3 HEPATORCH study, we aimed to compare the efficacy and safety of toripalimab plus bevacizumab versus sorafenib in patients with previously untreated advanced hepatocellular carcinoma.

Methods: We did a randomised, open-label, phase 3 study in 57 hospitals across mainland China, Taiwan, and Singapore.

Navigating the complexities: challenges and opportunities in conversion therapy for advanced hepatocellular carcinoma.

Primary liver cancer ranks as the sixth most prevalent malignant tumor and stands as the second leading cause of cancer-related mortality globally, posing a significant threat to public health. Hepatocellular carcinoma (HCC) is the most common type of liver cancer worldwide. Surgical resection remains the cornerstone treatment for achieving radical cure and prolonged survival in HCC patients.

Peripheral monocyte count is associated with the risk of liver metastasis: A study of 7187 newly diagnosed patients with colorectal cancer.

Objective: To evaluate the effect of the absolute peripheral blood monocyte count (AMC) on the risk of synchronous colorectal liver metastasis (synCRLM).

Materials And Methods: AMC as well as other baseline clinical characteristics between the synCRLM+ group (with synCRLM) and the synCRLM - group (without synCRLM) were measured. Taking AMC of 0.

PEG-based polyurethane bioadhesive for wet and adaptable adhesion to circumcision wounds.

Effective wound management is critical in post-operative recovery, particularly in sensitive areas such as circumcision. The aim of this study was to design and assess the efficacy of a novel two-component polyurethane (PU) bioadhesive, designated as PU1000S, for its application in advanced wound care within the context of clinical circumcision procedures. The glue-type bioadhesive was fine-tuned to conformally adhere to the moist tissue surfaces.

Hepatic artery infusion chemotherapy combined with camrelizumab and apatinib as conversion therapy for patients with unresectable hepatocellular carcinoma: a single-arm exploratory trial.

Background: The development of systemic therapy, including targeted drugs and immune checkpoint inhibitors, has significantly improved the prognosis of patients with advanced unresectable hepatocellular carcinoma (uHCC). Hepatic arterial infusion chemotherapy (HAIC) has been gradually applied to the treatment of advanced uHCC, showing good potential as conversion therapy. We aimed to investigate the efficacy and safety of HAIC combined with camrelizumab and apatinib as conversion therapy for uHCC.

[Study on the separation method of lung ventilation and lung perfusion signals in electrical impedance tomography based on rime algorithm optimized variational mode decomposition].

Real-time acquisition of pulmonary ventilation and perfusion information through thoracic electrical impedance tomography (EIT) holds significant clinical value. This study proposes a novel method based on the rime (RIME) algorithm-optimized variational mode decomposition (VMD) to separate lung ventilation and perfusion signals directly from raw voltage data prior to EIT image reconstruction, enabling independent imaging of both parameters. To validate this approach, EIT data were collected from 16 healthy volunteers under normal breathing and inspiratory breath-holding conditions.

Thermally Conductive TiCT Fibers with Superior Electrical Conductivity.

High-performance TiCT fibers have garnered significant potential for smart fibers enabled fabrics. Nonetheless, a major challenge hindering their widespread use is the lack of strong interlayer interactions between TiCT nanosheets within fibers, which restricts their properties. Herein, a versatile strategy is proposed to construct wet-spun TiCT fibers, in which trace amounts of borate form strong interlayer crosslinking between TiCT nanosheets to significantly enhance interactions as supported by density functional theory calculations, thereby reducing interlayer spacing, diminishing microscopic voids and promoting orientation of the nanosheets.

High-strength bone polyurethane adhesive with rapid curing for bone tissue injury repair.

Bone tissue adhesives have advantages such as preventing stress shielding and secondary surgical infections, fixing small bone fragments, easing operations, and enhancing wound adaptability. These methods can be applied for the traumatic repair of comminuted fractures. Currently, commercial tissue adhesives fail to meet the biological safety and mechanical strength requirements of bone tissue adhesives.

Engineered Extracellular Vesicles Derived from Juvenile Mice Enhance Mitochondrial Function in the Aging Bone Microenvironment and Achieve Rejuvenation.

Aging-related bone degeneration and impaired healing capacity remain significant challenges in regenerative medicine, necessitating innovative, efficient, and targeted strategies to restore bone health. Here, we engineered extracellular vesicles (EVs) derived from the serum of pretreated juvenile mice, with the goals of reversing aging, enhancing osteogenic potential, and increasing bioavailability to rejuvenate the aging bone environment. First, we established bone healing models representing different phases of healing to identify the EV type with the highest potential for improving the bone microenvironment in older individuals.

The Exserohilum turcicum effector EtEC81 reprograms alternative splicing in maize and activates immunity.

Some pathogen-derived effectors reprogram mRNA splicing in host plants to regulate plant immune responses. Whether effectors from Exserohilum turcicum, which causes northern corn leaf blight (NLB), interfere with RNA splicing remains unknown. We identify that the secreted protein EtEC81 (Exserohilum turcicum effector 81) modulates the alternative splicing (AS) of maize (Zea mays) pre-mRNAs and negatively regulates the pathogenicity of E.

Medical adhesives for soft tissue wound repair with good biocompatibility, flexibility, and high adhesive strength.

Compared with traditional soft tissue wound closure methods such as sutures and staplers, bioadhesives have significant advantages in terms of tissue compatibility, ease of use, and wound adaptability, making them a hot topic among current wound repair materials. This study aimed to select polyurethane materials with good biocompatibility and high mechanical strength, optimize the prepolymer formula, develop new curing agents, and obtain a new dual-component polyurethane bioadhesive. Furthermore, key research will be conducted on its mechanical properties and tissue adhesion performance.

BMP2 peptide-modified polycaprolactone-collagen nanosheets for periodontal tissue regeneration.

Introduction: Periodontitis leads to the degradation of tooth-supporting tissues, ultimately causing tooth mobility and loss. Guided tissue regeneration (GTR) surgery employs barrier membranes to facilitate tissue regeneration. However, conventional membranes lack bone-inducing properties, thereby limiting their efficacy.

3D-printed perfused models of the penis for the study of penile physiology and for restoring erectile function in rabbits and pigs.

The intricate topology of vascular networks and the complex functions of vessel-rich tissues are challenging to reconstruct in vitro. Here we report the development of: in vitro pathological models of erectile dysfunction and Peyronie's disease; a model of the penis that includes the glans and the corpus spongiosum with urethral structures; and an implantable model of the corpus cavernosum, whose complex vascular network is critical for erectile function, via the vein-occlusion effect. Specifically, we 3D printed a hydrogel-based corpus cavernosum incorporating a strain-limiting tunica albuginea that can be engorged with blood through vein occlusion.

Functionalized Aluminum Nitride for Improving Hydrolysis Resistances of Highly Thermally Conductive Polysiloxane Composites.

A series of divinylphenyl-acryloyl chloride copolymers (PDVB-co-PACl) is synthesized via atom transfer radical polymerization employing tert-butyl acrylate and divinylbenzene as monomers. PDVB-co-PACl is utilized to graft on the surface of spherical aluminum nitride (AlN) to prepare functionalized AlN (AlN@PDVB-co-PACl). Polymethylhydrosiloxane (PMHS) is then used as the matrix to prepare thermally conductive AlN@PDVB-co-PACl/PMHS composites with AlN@PDVB-co-PACl as fillers through blending and curing.

A novel method for detecting intracranial pressure changes by monitoring cerebral perfusion via electrical impedance tomography.

Background: Acute and critical neurological diseases are often accompanied with elevated intracranial pressure (ICP), leading to insufficient cerebral perfusion, which may cause severe secondary lesion. Existing ICP monitoring techniques often fail to effectively meet the demand for real-time noninvasive ICP monitoring and warning. This study aimed to explore the use of electrical impedance tomography (EIT) to provide real-time early warning of elevated ICP by observing cerebral perfusion.

Liquid Crystal-Engineered Polydimethylsiloxane: Enhancing Intrinsic Thermal Conductivity through High Grafting Density of Mesogens.

The increasing power and integration of electronic devices have intensified serious heat accumulation, driving the demand for higher intrinsic thermal conductivity in thermal interface materials, such as polydimethylsiloxane (PDMS). Grafting mesogens onto PDMS can enhance its intrinsic thermal conductivity. However, the high stability of the PDMS chain limits the grafting density of mesogens, restricting the improvement in thermal conductivity.