PEG-based polyurethane bioadhesive for wet and adaptable adhesion to circumcision wounds.

Effective wound management is critical in post-operative recovery, particularly in sensitive areas such as circumcision. The aim of this study was to design and assess the efficacy of a novel two-component polyurethane (PU) bioadhesive, designated as PU1000S, for its application in advanced wound care within the context of clinical circumcision procedures. The glue-type bioadhesive was fine-tuned to conformally adhere to the moist tissue surfaces.

High-strength bone polyurethane adhesive with rapid curing for bone tissue injury repair.

Bone tissue adhesives have advantages such as preventing stress shielding and secondary surgical infections, fixing small bone fragments, easing operations, and enhancing wound adaptability. These methods can be applied for the traumatic repair of comminuted fractures. Currently, commercial tissue adhesives fail to meet the biological safety and mechanical strength requirements of bone tissue adhesives.

Medical adhesives for soft tissue wound repair with good biocompatibility, flexibility, and high adhesive strength.

Compared with traditional soft tissue wound closure methods such as sutures and staplers, bioadhesives have significant advantages in terms of tissue compatibility, ease of use, and wound adaptability, making them a hot topic among current wound repair materials. This study aimed to select polyurethane materials with good biocompatibility and high mechanical strength, optimize the prepolymer formula, develop new curing agents, and obtain a new dual-component polyurethane bioadhesive. Furthermore, key research will be conducted on its mechanical properties and tissue adhesion performance.

Antimicrobial Peptide- and Dentin Matrix-Functionalized Hydrogel for Vital Pulp Therapy via Synergistic Bacteriostasis, Immunomodulation, and Dentinogenesis.

The preservation of vital pulps is crucial for maintaining the physiological functions of teeth; however, vital pulp therapy (VPT) of pulpitis teeth remains a substantial challenge due to uncontrolled infection, excessive inflammation, and limited regenerative potential. Current pulp capping agents have restricted effects in the infectious and inflammatory microenvironment. To address this, a multifunctional hydrogel (TGH/DM) with antibacterial, immunomodulatory, and mineralization-promoting effects is designed.

Mechanical Stimulation of Anti-Inflammatory and Antioxidant Hydrogels for Rapid Re-Epithelialization.

The epithelium, an essential barrier to protect organisms against infection, exists in many organs. However, rapid re-epithelialization to restore tissue integrity and function in an adverse environment is challenging. In this work, a long-term anti-inflammatory and antioxidant hydrogel with mechanical stimulation for rapid re-epithelialization, mainly composed of the small molecule thioctic acid, biocompatible glycine, and γ-FeO nanoparticles is reported.

A sandwiched patch toward leakage-free and anti-postoperative tissue adhesion sealing of intestinal injuries.

Ideal repair of intestinal injury requires a combination of leakage-free sealing and postoperative antiadhesion. However, neither conventional hand-sewn closures nor existing bioglues/patches can achieve such a combination. To this end, we develop a sandwiched patch composed of an inner adhesive and an outer antiadhesive layer that are topologically linked together through a reinforced interlayer.

Functional reconstruction of injured corpus cavernosa using 3D-printed hydrogel scaffolds seeded with HIF-1α-expressing stem cells.

Injury of corpus cavernosa results in erectile dysfunction, but its treatment has been very difficult. Here we construct heparin-coated 3D-printed hydrogel scaffolds seeded with hypoxia inducible factor-1α (HIF-1α)-mutated muscle-derived stem cells (MDSCs) to develop bioengineered vascularized corpora. HIF-1α-mutated MDSCs significantly secrete various angiogenic factors in MDSCs regardless of hypoxia or normoxia.

Wet-adhesive, haemostatic and antimicrobial bilayered composite nanosheets for sealing and healing soft-tissue bleeding wounds.

Healing soft-tissue wounds with an irregular, complicated topography in a bleeding environment demands the development of a dressing that is wet-adhesive, haemostatic, and antibacterial. To meet this unmet demand, we designed a flexible nanosheet (~77 nm thick) made of two layers, one is the antibacterial and haemostatic gelatin modified with dopamine (DA) and antimicrobial peptide (AMP) and mixed with Ca ions as coagulation factors, and another is the mechanically strong polycaprolactone (PCL). This flexible nanosheet exhibited robust mechanical strength, continuous and effective adhesion to a topographically irregular tissue surface under a wet condition, and a high platelet adhesion capacity.

A Biomimetic Biphasic Osteochondral Scaffold with Layer-Specific Release of Stem Cell Differentiation Inducers for the Reconstruction of Osteochondral Defects.

There is a great challenge in regenerating osteochondral defects because they involve lesions of both cartilage and subchondral bone, which have remarkable differences in their chemical compositions and biological lineages. Thus, considering the complicated requirements in osteochondral reconstruction, a biomimetic biphasic osteochondral scaffold (BBOS) with the layer-specific release of stem cell differentiation inducers are developed. The cartilage regeneration layer (cartilage scaffold, CS) in the BBOS contains a hyaluronic acid hydrogel to mimic the composition of cartilage, which is mechanically enhanced by host-guest supramolecular units to control the release of kartogenin (KGN).

Molecular recognition-directed site-specific release of stem cell differentiation inducers for enhanced joint repair.

The remarkable difference in cell type and matrix composition between two connected parts of a joint (cartilage and subchondral bone) makes it challenging to simultaneously regenerate both parts for joint repair. Thus we chemically designed a biphasic hydrogel made of two well-bonded shape-tunable hydrogel phases, termed bone-regenerating hydrogel (BRH) and cartilage-regenerating hydrogel (CRH). The BRH and CRH, encapsulating stem cells, were produced by photo-crosslinking bone and cartilage matrix-mimicking biopolymers and a nanobox (β-cyclodextrin) in situ in the subchondral bone defect and cartilage defect, respectively.

Di-n-butyl phthalate exposure negatively influences structural and functional neuroplasticity via Rho-GTPase signaling pathways.

Di-n-butyl phthalate (DBP) has been reported to cause disruptions in hippocampal plasticity, but its specific mechanism has not yet been ascertained. In this research, a mouse model of chronic DBP exposure was generated by intragastric administration of DBP (10, 50, or 250°mg/kg/d) for 5 weeks. Chronic exposure to high concentrations of DBP (250°mg/kg/d) induced a spatial learning deficit in the Morris water maze in male mice.