Multifaceted Biodiversity Patterns and Influencing Factors of Stag Beetles (Coleoptera, Lucanidae) in China.

As keystone decomposers in forest ecosystems, stag beetles occupy unique ecological niches within forest carbon and nitrogen cycles. While previous studies have predominantly focused on taxonomic inventories of this group, we present a multidimensional assessment integrating taxonomic, phylogenetic, and functional diversity patterns across the model genus of stag beetles in China. Using random forest analyses, we identify key environmental factors influencing these biodiversity dimensions-contemporary climate factors, habitat heterogeneity, and paleoclimatic fluctuations.

C/EBP β/AEP pathway mediates hippocampal mitochondrial damage in a mouse model of sepsis encephalopathy.

Septic encephalopathy (SE), a neurocritical complication with alarmingly high mortality rates, represents a major diagnostic and therapeutic challenge in intensive care units (ICUs). Current diagnostic protocols rely predominantly on nonspecific clinical symptom assessment, while conventional laboratory biomarkers exhibit insufficient sensitivity for early detection. This diagnostic ambiguity contributes to delayed interventions and unfavorable patient prognoses.

Erdafitinib inhibits the tumorigenicity of MDA-MB-231 triple-negative breast cancer cells by inducing TRIM25/ubiquitin-dependent degradation of FGFR4.

Triple-negative breast cancer (TNBC) is the most malignant subtype of breast cancer (BC), characterized by limited treatment options and poor clinical outcomes. Aberrant FGFR signaling has been implicated in TNBC; however, the therapeutic potential of targeting FGFRs for TNBC treatment remains unclear. This study investigated the anti-cancer activity of the selective pan-FGFR inhibitor Erdafitinib and its underlying mechanisms using both in vitro and in vivo models.

Pan-cancer analysis and validation of NT5DC2: emphasizing its prognostic significance and immunological role in TNBC.

Background: 5'-Nucleotidase Domain Containing 2 (NT5DC2), a member of the cNT5-II family that catalyzes nucleotide hydrolysis, plays a critical role in tumor initiation and progression. This study aims to elucidate the role of NT5DC2 across multiple cancers and to confirm its oncogenic significance in triple-negative breast cancer (TNBC).

Methods: NT5DC2 expression patterns were analyzed across multiple databases to evaluate its diagnostic and prognostic value in various cancers.

A Comprehensive Bioinformatic Analysis Based on Functional Studies of MEF-2 Family in NSCLC.

Lung cancer remains the malignancy with the highest morbidity and mortality worldwide. There are no effective guiding therapies and prognosis biomarkers, and the overall prognosis of lung cancer remains poor. The cardiomyocyte enhancer factor 2 (MEF-2) family is a highly evolutionarily conserved transcription factor that plays important roles in a variety of diseases, including tumors.

Effect of atmospheric-pressure cold plasma (ACP) treatment on the structure and function of WPI-EGCG complexes.

In this study, atmospheric pressure cold plasma (ACP) technology was used to assist in the construction of whey protein isolate-epigallocatechin gallate non-covalent complexes (WPI-EGCG). The mechanism by which ACP treatment influences the conformational relationship of the complexes was systematically analyzed. The EGCG binding ratio, free amino and sulfhydryl content, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) changes confirmed that the ACP treatment was able to significantly enhance the non-covalent interactions between WPI-EGCG through the oxidative modification and molecular cross-linking induced by the activated particles.

Spatiotemporal evolution of agricultural carbon emissions intensity in China and analysis of influencing factors.

This study aims to investigate the spatiotemporal evolution of China's agricultural carbon emissions intensity and their influencing factors from 2001 to 2022. It employs analytical methods such as kernel density estimation, Gini coefficient, Moran's index, spatial Markov analysis, and spatiotemporal geographically weighted regression (GWTR) to visualize the data and analyze the results. The study ultimately draws the following.

ITGAV, a specific biomarker associated with the pathogenesis of idiopathic pulmonary fibrosis.

Objective Idiopathic pulmonary fibrosis (IPF), which radiologically/pathologically manifests mainly as usual interstitial pneumonia (UIP), is easily confused with chronic hypersensitivity pneumonitis (CHP) and collagenous vascular disease in clinical settings, affecting the physician's diagnosis and treatment. Accurate identification of IPF from various diseases presenting as UIP is essential for effective diagnosis and therapy. Methods Gene expression data of CHP, IPF, and rheumatoid arthritis-UIP samples were downloaded from the GEO database, and specific biomarkers were identified to differentiate idiopathic UIP/IPF from secondary UIP.

Microbiome catalog and dynamics of the Chinese liquor fermentation process.

Fermented food remains poorly understood, largely due to the lack of knowledge about microbes in food fermentation. Here, this study constructed Moutai Fermented Grain Catalog (MTFGC), a representative liquor fermented by one of the most complex fermentations. MTFGC comprised 8,379,551 non-redundant genes and 5,159 metagenome-assembled genomes, with 20% species and 20% genes being novel.

Design and Evaluation of Novel Peptide-Based PET Probe for Noninvasive Imaging of HER2 Expression in Breast Cancer.

The overexpression of HER2 has been strongly correlated with the pathogenesis and progression of breast cancer. Developing HER2-targeted PET tracers for noninvasive assessment of HER2 expression holds substantial clinical significance for screening patients suitable for HER2-targeted therapy, evaluating treatment efforts, and optimizing treatment. In this study, we employed computational simulation methods to develop peptide WC8 derived from trastuzumab, and designed HER2 targeting radiotracers [Ga]Ga-DOTA-WC8 for detecting the HER2 expression.

A novel gemcitabine analog as a potential anticancer agent: synthesis and evaluation against pancreatic cancer.

Gemcitabine (Gem) is approved for use in pancreatic cancer chemotherapy. However, Gem undergoes rapid metabolism in the blood, producing an inactive metabolite. Due to this rapid metabolism, the effective dose of Gem is high, thereby predisposing patients to severe adverse effects.

Association between genetic prediction of 486 blood metabolites and the risk of idiopathic pulmonary fibrosis: A mendelian randomization study.

Metabolic disorders are a significant feature of fibrotic diseases. Nevertheless, the lack of sufficient proof regarding the cause-and-effect association between circulating metabolites and the promotion or prevention of idiopathic pulmonary fibrosis (IPF) persists. To assess the causal association between IPF and genetic proxies of 486 blood metabolites, a dual sample Mendelian randomization (MR) analysis was performed.

Synthesis of Oxazoles Containing CF-Substituted Alcohol Unit via Tandem Cycloisomerization/Hydroxyalkylation from -Propargylamides with Trifluoropyruvates.

Oxazoles are important five-membered heterocycles that contain both nitrogen and oxygen atoms. Due to their wide range of biological activities, many oxazoles demonstrate potential for extensive application in various fields, including medicinal chemistry. Trifluoromethyl carbinol, an important pharmacophore, contains both trifluoromethyl and hydroxyl groups and is common in molecules with important biological activities.

Pyrotinib induces cell death in HER2-positive breast cancer via triggering HSP90-dependent HER2 degradation and ROS/HSF-1-dependent oxidative DNA damage.

HER2-positive breast cancer (HER2+ BC) is distinguished by its poor prognosis, propensity for early onset, and high risk of recurrence and metastasis. Consequently, anti-HER2-targeted therapy has emerged as a principal strategy in the treatment of this form of breast cancer. Pyrotinib, a novel irreversible pan-HER2 tyrosine kinase inhibitor, has brought fresh hope to patients with advanced HER2+ breast cancer.

The regulation of NFKB1 on CD200R1 expression and their potential roles in Parkinson's disease.

Background: Overactivated microglia are a key contributor to Parkinson's disease (PD) by inducing neuroinflammation. CD200R1, a membrane glycoprotein mainly found on microglia, is crucial for maintaining quiescence with its dysregulation linked to microglia's abnormal activation. We and other groups have reported a decline in CD200R1 levels in several neurological disorders including PD.

STAT3 inhibitor Stattic Exhibits the Synergistic Effect with FGFRs Inhibitor Erdafitinib in FGFR1-positive Lung Squamous Cell Carcinoma.

Lung squamous cell carcinoma (LUSC), a subset of non-small cell lung cancer (NSCLC), accounts for about 30% of all lung cancers (LC) and exhibits a dismal response to current therapeutic protocols. Existed studies have indicated that aberrations in fibroblast growth factor receptors (FGFRs) play a pivotal role in the progression of LUSC, rendering them as attractive targets for therapeutic intervention in this cancer type. This study found that Erdafitinib (Erda), a novel pan-FGF receptor tyrosine kinase inhibitor (TKI), exerted a cytotoxic effect on LUSC cells.

The diagnostic value of Ga-NOTA-MAL-Cys-MZHER PET/CT imaging for HER2-positive lung adenocarcinoma.

Background: The human epidermal growth factor receptor 2 gene (HER2) has been identified as a potential therapeutic target in lung adenocarcinoma (LUAD). Non-invasive positron emission tomography (PET) imaging provides a reliable strategy for determination of HER2 expression through whole-body detection of abnormalities. The PET tracer Ga-NOTA-MAL-Cys-MZHER has shown promising results for HER2-positive breast and gastric cancers.

The RAE1-STOP1-GL2-RHD6 module regulates the ALMT1-dependent aluminum resistance in Arabidopsis.

Aluminum (Al) toxicity is one of the major constraints for crop production in acid soils, Al-ACTIVATED MALATE TRANSPORTER1 (ALMT1)-dependent malate exudation from roots is essential for Al resistance in Arabidopsis, in which the C2H2-type transcription factor SENSITIVE TO PROTONRHIZOTOXICITY1 (STOP1) play a critical role. In this study, we reveal that the RAE1-GL2-STOP1-RHD6 protein module regulated the ALMT1-mediated Al resistance. GL2, STOP1 and RHD6 directly target the promoter of ALMT1 to suppress or activate its transcriptional expression, respectively, and mutually influence their action on the promoter of ALMT1 by forming a protein complex.

Erdafitinib promotes ferroptosis in human uveal melanoma by inducing ferritinophagy and lysosome biogenesis via modulating the FGFR1/mTORC1/TFEB signaling axis.

Uveal melanoma (UM) is a rare yet lethal primary intraocular malignancy affecting adults. Analysis of data from The Cancer Genome Atlas (TCGA) database revealed that FGFR1 expression was increased in UM tumor tissues and was linked to aggressive behavior and a poor prognosis. This study assessed the anti-tumor effects of Erdafitinib, a selective pan-FGFR inhibitor, in both in vitro and in vivo UM models.

Ultrasensitive detection of uveal melanoma using [F]AlF-NOTA-PRGD2 PET imaging.

Background: Uveal melanoma (UM) is the most common primary intraocular tumor in adults, and early detection is critical to improve the clinical outcome of this disease. In this study, the diagnostic effectiveness of [F]AlF-NOTA-PRGD2 (an investigational medicinal product) positron emission tomography (PET) imaging in UM xenografts and UM patients were evaluated. The cell uptake, cell binding ability and in vitro stability of [F]AlF-NOTA-PRGD2 were evaluated in 92-1 UM cell line.