Reversible increased basement membrane permeability and calcium ion redistribution facilitate ultrasound-enhanced transdermal drug delivery efficiency.

Background: Ultrasound-assisted transdermal drug delivery, or sonophoresis, enhances skin permeability, offering a non-invasive alternative for drug administration. However, its clinical application remains limited because of an insufficient understanding of its underlying mechanisms and optimal parameters. This study investigates the factors influencing ultrasound-enhanced drug absorption and examines its biological effects on skin structures and HaCaT cells, providing a comprehensive analysis of its mechanisms.

Heterologous expression of L-lactate dehydrogenase in Chlorella pyrenoidosa: A beginning for poly(L-lactic acid) biosynthesis from CO by eukaryotic microalgae.

Lactic acid is a monomer of poly(lactic acid), and the use of sugar-based raw materials in conventional lactic acid production poses a risk to food security. CO is an abundant carbon resource, and microalgae are crucial for the development of third-generation bio-manufacturing through CO utilization. In this study, the transformation efficiency of Chlorella pyrenoidosa (C.

Assessment of Pesticide Residues and Dietary Risks in Ginseng from Northeastern China.

Several challenges persist in China's ginseng industry. Phoxim, chlorpyrifos, quintozene-unregistered pesticides primarily used as soil insecticides and fungicides-may pose high dietary risks. This study performed a thorough screening of potential pesticides used in Chinese ginseng cultivation, evaluated the long-term dietary risks for the ginseng-consuming group, and used the primary risk factors for ginseng in China and South Korea to compare the two nations' pesticide usage scenarios.

Kir4.1/Kir5.1 of distal convoluted tubule is required for short-term angiotensin-II-induced stimulation of Na-Cl cotransporter.

Angiotensin-II (Ang-II) perfusion stimulates inwardly-rectifying potassium channels 4.1 and 5.1 (Kir4.

Determination of Cyazofamid and Its Metabolite in Rice Based on Magnetic Zirconia Nanoparticles Sample Cleanup and Its Application to a Dissipation and Residue Study.

An analytical method was developed for the determination of cyazofamid (CZFM) and its metabolite CCIM in rice, employing magnetic zirconia nanoparticles (MZNPs) for sample cleanup. MZNPs were synthesized through a one-step hydrothermal process and characterized by multiple techniques. Samples of rice plant, rice hull, and brown rice were extracted using acetonitrile/water, followed by salting out.

[Effects of high salt diet on renal vascular reactivity in mice detected by laser speckle contrast imaging].

The aim of this study was to conduct experiments using laser speckle contrast imaging (LSCI) technology to investigate the effects of high salt diet on renal vascular reactivity in mice. LSCI is a technology for monitoring blood flow based on the laser speckle principle. It has been widely used to detect microcirculatory functions in tissues such as the skin and brain.

In-situ online investigation of biogenic volatile organic compounds emissions from tropical rainforests in Hainan, China.

Biogenic volatile organic compounds (BVOCs) emitted by tropical plants represent a significant proportion of global emissions, but the in-situ BVOC measurements in tropical rainforests are extremely sparse. Herein, a vehicle-mounted mobile monitoring system was developed for in-situ online investigations of BVOC emissions from thirty representative tree species in the tropical rainforests of Hainan Island, southern China. The results showed that monoterpenes were the primary BVOCs emitted from most broadleaf trees.

Role of Kir4.1/Kir5.1 in mediating Angiotensin-II (Ang-II)-induced stimulation of thiazide-sensitive Na-Cl cotransporter.

Background: Angiotensin-II (Ang-II) perfusion stimulates Kir4.1/Kir5.1 in the distal-convoluted-tubule (DCT) and thiazide-sensitive Na-Cl-cotransporter (NCC).

The development of local ambient air quality standards: A case study of Hainan Province, China.

The ambient air quality standard (AAQS) is a vital policy instrument for protecting the environment and human health. Hainan Province is at the forefront of China's efforts to protect its ecological environment, with an official goal to achieve world-leading air quality by 2035. However, neither the national AAQS nor the World Health Organization guideline offers sufficient guidance for improving air quality in Hainan because Hainan has well met the former while the latter is excessively stringent.

The diverse effects of cisplatin on tumor microenvironment: Insights and challenges for the delivery of cisplatin by nanoparticles.

Cisplatin is a well-known platinum-based chemotherapy medication that is widely utilized for some malignancies. Despite the direct cytotoxic consequences of cisplatin on tumor cells, studies in the recent decade have revealed that cisplatin can also affect different cells and their secretions in the tumor microenvironment (TME). Cisplatin has complex impacts on the TME, which may contribute to its anti-tumor activity or drug resistance mechanisms.

Transcriptome profiling in rumen, reticulum, omasum, and abomasum tissues during the developmental transition of pre-ruminant to the ruminant in yaks.

The development of the four stomachs of yak is closely related to its health and performance, however the underlying molecular mechanisms are largely unknown. Here, we systematically analyzed mRNAs of four stomachs in five growth time points [0 day, 20 days, 60 days, 15 months and 3 years (adult)] of yaks. Overall, the expression patterns of DEmRNAs were unique at 0 d, similar at 20 d and 60 d, and similar at 15 m and adult in four stomachs.

The role of NO in Co-occurrence of O and PM pollution driven by wintertime east Asian monsoon in Hainan.

Clarifying the driving forces of O and fine particulate matter (PM) co-pollution is important to perform their synergistic control. This work investigated the co-pollution of O and PM in Hainan Province using an observation-based model and explainable machine learning. The O and PM pollution that occurs in winter is affected by the wintertime East Asian Monsoon.

Housing investment and family entrepreneurship: Evidence from China.

The aim of this article is to explore the impact of housing investment on household entrepreneurship. Using survey data from China and employing a Probit model, we examine three aspects of housing status and innovatively subdivide household entrepreneurship into agricultural entrepreneurship and business entrepreneurship. The results show that households with higher housing investment are less likely to become agricultural entrepreneurs, but more likely to start a new business.

Low-Dose Radiation Reduces Doxorubicin-Induced Myocardial Injury Through Mitochondrial Pathways.

The use of doxorubicin (DOX) as an anthraquinone antineoplastic agent is limited due to its cardiotoxicity. Our previous study suggested that low-dose radiation (LDR) could mitigate the cardiotoxicity induced by DOX via suppressing oxidative stress and cell apoptosis. However, the molecular targets and protective mechanism of LDR are not understood.

Assessing Population Structure and Signatures of Selection in Wanbei Pigs Using Whole Genome Resequencing Data.

Wanbei pig (WBP) is one of the indigenous pig resources in China and has many germplasm characteristics. However, research on its genome is lacking. To assess the genomic variation, population structure, and selection signatures, we resequenced 18 WBP for the first time and performed a comprehensive analysis with resequenced data of 10 Asian wild boars.

The effect of high-dietary K (HK) on Kir4.1/Kir5.1 and ROMK in the distal convoluted tubule (DCT) is not affected by gender and Cl content of the diet.

Basolateral potassium channels in the distal convoluted tubule (DCT) are composed of inwardly-rectifying potassium channel 4.1 (Kir4.1) and Kir5.

Population Structure and Selection Signatures Underlying Domestication Inferred from Genome-Wide Copy Number Variations in Chinese Indigenous Pigs.

Single nucleotide polymorphism was widely used to perform genetic and evolution research in pigs. However, little is known about the effect of copy number variation (CNV) on characteristics in pigs. This study performed a genome-wide comparison of CNVs between Wannan black pigs (WBP) and Asian wild boars (AWB), using whole genome resequencing data.

Role of low-dose radiation in senescence and aging: A beneficial perspective.

Cellular senescence refers to the permanent arrest of cell cycle caused by intrinsic and/or extrinsic stressors including oncogene activation, irradiation, DNA damage, oxidative stress, and certain cytokines (including senescence associated secretory phenotype). Cellular senescence is an important factor in aging. Accumulation of senescent cells has been implicated in the causation of various age-related organ disorders, tissue dysfunction, and chronic diseases.

[The mechanism of blood pressure regulation by high potassium diet in the kidney].

Hypertension is one of the strongest risk factors for cardiovascular diseases, cerebral stroke, and kidney failure. Lifestyle and nutrition are important factors that modulate blood pressure. Hypertension can be controlled by increasing physical activity, decreasing alcohol and sodium intake, and stopping tobacco smoking.

The Research of Tool Wear Mechanism for High-Speed Milling ADC12 Aluminum Alloy Considering the Cutting Force Effect.

Tool wear is a major cause of accelerated tool failure during the milling of aluminum alloy. The periodically cutting force directly affect the cutting heat and tool wear due to the intermittent cutting characteristics of the milling process. The focus of this paper is to analyze the influence of the variation of cutting force on tool wear behavior.

PGF stimulates the 10-pS Cl channel and thiazide-sensitive Na-Cl cotransporter in the distal convoluted tubule.

We used patch-clamp and Western blot analysis to test whether PGF stimulates the basolateral 10-pS Cl channel and thiazide-sensitive Na-Cl cotransporter (NCC) in the distal convoluted tubule (DCT) via a prostaglandin F receptor (FP-R). Single channel and whole cell recordings demonstrated that PGF stimulated the 10-pS Cl channel in the DCT. The stimulatory effect of PGF on the Cl channel was mimicked by a FP-R agonist, latanoprost, but was abrogated by blocking FP-R with AL8810.

Inhibition of AT2R and Bradykinin Type II Receptor (BK2R) Compromises High K Intake-Induced Renal K Excretion.

The inhibition of Type II angiotensin II receptor (AT2R) or BK2R (bradykinin type II receptor) stimulates basolateral Kir4.1/Kir5.1 in the distal convoluted tubule (DCT) and activates thiazide-sensitive NCC (Na-Cl cotransporter).

Low-Dose Radiation Enhanced Inhibition of Breast Tumor Xenograft and Reduced Myocardial Injury Induced by Doxorubicin.

We reported that low-dose radiation (LDR) alleviated cardiotoxicity of doxorubicin (DOX) via inhibiting myocardial cell apoptosis and oxidative stress in vivo. Here, we tested whether LDR could enhance chemotherapeutic effect of DOX and alleviate myocardial injury induced by DOX by observing cell proliferation, apoptosis, and metastasis of heterotopic tumor in vivo. Mice implanted with 4T1 breast carcinoma cells were given 7.

Norepinephrine-Induced Stimulation of Kir4.1/Kir5.1 Is Required for the Activation of NaCl Transporter in Distal Convoluted Tubule.

The stimulation of β-adrenergic receptor increases thiazide-sensitive NaCl cotransporter (NCC), an effect contributing to salt-sensitive hypertension by sympathetic stimulation. We now test whether the stimulation of β-adrenergic receptor-induced activation of NCC is achieved through activating basolateral Kir4.1 in the distal convoluted tubule (DCT).