Publications by authors named "Cheng-Biao Zhang"
Kir4.1/Kir5.1 of distal convoluted tubule is required for short-term angiotensin-II-induced stimulation of Na-Cl cotransporter.
Am J Physiol Renal Physiol
June 2025
Angiotensin-II (Ang-II) perfusion stimulates inwardly-rectifying potassium channels 4.1 and 5.1 (Kir4.
View Article and Find Full Text PDFArticle Synopsis
- Kir5.1, when paired with Kir4.2, forms a crucial potassium channel (heterotetramer) in the basolateral membrane of mouse proximal tubules, affecting K+ conductance.
- Immunofluorescence and immunoblotting show Kir4.2 is found exclusively in proximal tubules, while Kir5.1 is present in both proximal and distal nephrons; however, the absence of Kir5.1 reduces Kir4.2 levels and affects membrane staining.
- Patch-clamp recordings reveal that Kir5.1-knockout mice lack the 50-pS K channel that is present in wild-type mice, leading to a less negative membrane potential in the proximal tubules, indicating the importance
Background: Angiotensin-II (Ang-II) perfusion stimulates Kir4.1/Kir5.1 in the distal-convoluted-tubule (DCT) and thiazide-sensitive Na-Cl-cotransporter (NCC).
View Article and Find Full Text PDFAngiotensin II-Type-1a Receptor and Renal K + Wasting during Overnight Low-Na + Intake.
J Am Soc Nephrol
November 2024
Key Points: Angiotensin II–type-1a-receptor in the distal convoluted tubule (DCT) plays a role in regulating sodium transport in the DCT. Angiotensin II–type-1a-receptor in the DCT plays a role in maintaining potassium homeostasis during sodium restriction.
Background: Chronic angiotensin II perfusion stimulates Kir4.
Calcineurin, protein phosphatase 2B (PP2B) or protein phosphatase 3 (PP3), is a calcium-dependent serine/threonine protein phosphatase. Calcineurin is widely expressed in the kidney and regulates renal Na and K transport. In the thick ascending limb, calcineurin plays a role in inhibiting NKCC2 function by promoting the dephosphorylation of the cotransporter and an intracellular sorting receptor, called sorting-related-receptor-with-A-type repeats (SORLA), is involved in modulating the effect of calcineurin on NKCC2.
View Article and Find Full Text PDF[Recording and identification of depolarization-activated current in intercalated cells].
Sheng Li Xue Bao
February 2024
The depolarization-activated current of intercalated cells in the distal nephron was detected for the first time, and the type of ion channel mediating the current was identified based on electrophysiological and pharmacological properties. The whole-cell current of distal nephron in kidney of C57BL/6J mice was recorded by Axon MultiClamp 700B patch-clamp system, and the effects of several K channel inhibitors on the depolarization-activated current in intercalated cells were observed. In addition, the immunofluorescence technique was used to investigate the localization of the channel in intercalated cells.
View Article and Find Full Text PDF[A method of acutely isolating rat dorsal root ganglion neurons for patch-clamp study of single-channel].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
November 2011
Objective: To establish a method of acutely isolating dorsal root ganglion (DRG) neurons for patch clamp study of single-channel.
Methods: DRG neurons of rats were acutely isolated by enzymatic digestion and mechanical blowing.
Results: The acutely isolated DRG cells were easy to form the higher sealing resistance (> 5G Omega), which lowered noise level, so that pA-level single channel currents could be recorded.