Activation-Triggered Lysosome-Retaining Visible/NIR-II Integrated Fluorescent Probe Enables Tracking of Lysosomal Changes during Ferroptosis in Cells and .

Lysosomes are the key organelles in regulating cellular homeostasis, posing a significant role from basic enzyme trafficking to cell death. Existing fluorescent probes for imaging of lysosomes have limitations, such as tumor-associated false positives due to pH-dependent retention and single-channel emission properties restricting their use to either cellular or imaging. Real-time and high-fidelity visualization of lysosomal changes during ferroptosis both at cells and the tumor level presents a big challenge.

Non-equilibrium dynamics in geometrically frustrated spin glass BiFeGaOwith a Cairo lattice.

We have explored the magnetic relaxation of the spin glass phase in BiFeGaO, a geometrically frustrated magnet with a unique Cairo lattice made of pentagonal building blocks. Using dc magnetization measurements, we find the relaxation behavior in single crystals follows the Arrhenius law as a function of temperature but remains relatively constant under different magnetic fields. Through a carefully designed protocol, we observe significant rejuvenation and memory effects, reminiscent of classical dilute spin glasses rather than spin jamming systems.

Production of live monkeys and their genetically matched embryonic stem cells from single embryos.

Immune rejection poses a challenge in stem cell therapy, especially with allogeneic embryonic stem cells (ESCs). Non-human primates offer a promising avenue for developing genetically matched ESCs for regenerative medicine. Here, we successfully derive three live monkeys and their genetically matched autologous ESCs (aESCs) using embryo splitting.

[Application of three-dimensional reconstruction technology in preoperative planning of anterolateral thigh flap transplantation].

Objective: To investigate the application of three-dimensional (3D) reconstruction technology in preoperative planning for anterolateral thigh flap transplantation.

Methods: A retrospective analysis was performed on the clinical data of 11 patients with skin and soft tissue defects treated with free anterolateral thigh flap transplantation between January 2022 and January 2024, who met the selection criteria. There were 8 males and 3 females, aged 34-70 years (mean, 50.

Flavanol-hydroxytyrosol hybrids and diterpenoids with anti-neuroinflammatory activity by regulating the expressions of NF-κB and IL-1β in vitro from Tripterygium hypoglaucum rhizomes.

The study aimed to identify the anti-neuroinflammatory compounds present in the rhizomes of Tripterygium hypoglaucum (Levl.) Hutch while simultaneously elucidating their mechanisms of action against neuroinflammation in vitro. Seven previously undescribed compounds, including a pair of flavanol-hydroxytyrosol hybrids (1-2) and three diterpenoids (5-7), were isolated from T.

11,12-Diacetyl-Carnosol Ameliorates Depression-Like Behaviors and Memory Dysfunction in CUMS Mouse Model via Inhibiting HMGB1-Mediated Neuroinflammation.

Backgrounds: 11,12-Diacetyl-carnosol (DACA), a derivative of carnosol, exhibits significant anti-inflammatory and antioxidant properties. However, its antidepressant effects and underlying mechanisms remain unclear. High mobility group box 1 protein (HMGB1)-mediated inflammatory responses and associated neurofunctional impairments play a crucial role in the pathogenesis of depression.

Altermagnetism in the layered intercalated transition metal dichalcogenide CoNbSe.

Altermagnets (AMs) are a new class of magnetic materials that combine the beneficial spintronics properties of ferromagnets and antiferromagnets, garnering significant attention recently. Here, we have identified altermagnetism in a layered intercalated transition metal diselenide, CoNbSe, which crystallizes with an ordered sublattice of intercalated Co atoms between NbSe layers. Single crystals are synthesized, and the structural characterizations are performed using single crystal diffraction and scanning tunneling microscopy.

Characterization and Isolation of Ginger Phenols from Rhizomes through in Silico-Based Reverse Annotation and Molecular Networking.

Ginger is a rich source of ginger phenols with an anti-inflammatory property. Antineuroinflammatory pathways represent a promising therapeutic strategy for neurodegenerative diseases. Ginger was selected as our research object to discover bioactive constituents responsible for the antineuroinflammatory effect.

Enhancing clinical precision in lung cancer tissue biopsy through elevated response-threshold of an endoplasmic reticulum-targeted fluorogenic probe.

Lung carcinoma is the leading cause of mortality globally, posing a significant public health concern. Fluorescent-mediated tumor imaging is emerging as a novel diagnostic and therapeutic approach in clinical practice. Nevertheless, traditional probes lack accuracy in diagnosing tumors due to the overlap in baseline values of certain tumor biomarkers between normal and tumor cells as both exhibit turn-on fluorescence, rendering it impossible to distinguish tumor tissue from normal tissue with high resolution.

Unveiling the multifaceted benefits of Simiao Pill in ulcerative colitis: Integrative analysis of signaling pathways, gut microbiota, and lipid metabolism.

Ethnopharmacological Relevance: Ulcerative colitis (UC), a chronic idiopathic inflammatory bowel disease, is characterized by a prolonged and recurrent course. The classical Traditional Chinese Medicine (TCM) formula Simiao Pill (SMP) has demonstrated unique and widespread therapeutic effects on diarrhea and other intestinal inflammation. However, its material basis and potential mechanisms of action remain unclear.

Microbiome-Metabolomics Analysis of the Impacts of Balantidium Coli Infection in Rhesus Monkeys (Macaca mulatta).

Balantidium coli (B. coli) is a prevalent intestinal parasite in monkeys, significantly impacting their health. Previous studies have demonstrated that B.

Research on the Evolution Characteristics and Influencing Factors of Foamy Oil Bubbles in Porous Media.

This study systematically investigates the formation mechanism and development characteristics of the "foamy oil" phenomenon during pressure depletion development of high-viscosity crude oil through a combination of physical experiments and numerical simulations. Using Venezuelan foamy oil as the research subject, an innovative heterogeneous pore-etched glass model was constructed to simulate the pressure depletion process, revealing for the first time that bubble growth predominantly occurs during the migration stage. Experimental results demonstrate that heavy components significantly delay degassing by stabilizing gas-liquid interfaces, while the continuous gas-liquid diffusion effect explains the unique development characteristics of foamy oil-high oil recovery and delayed phase transition-from a microscopic perspective.

Imaging and Pathological Comprehensive Analysis of Oesophageal Squamous Cell Carcinoma in a Rhesus Monkey (Macaca mulatta).

Squamous cell carcinoma (SCC) has a high incidence in non-human primates (NHPs), which affects animal health and causes huge losses to the experimental monkey breeding industry. In this case, gastroscope showed a smooth-surfaced elevated lesion located approximately 18 cm from the incisor teeth of the diseased rhesus monkey. Computed tomography (CT) showed a space-occupying lesion in the oesophagus.

Unveiling the hepatoprotective mechanisms of Desmodium heterocarpon (L.) DC: Novel flavonoid identification and Keap1/Nrf2 pathway activation.

Background: The pathophysiology of liver diseases is significantly influenced by oxidative stress, making its alleviation a key strategy for treatment. The Keap1/Nrf2 signaling pathway is the body's most crucial antioxidant defense mechanism. Traditional Chinese medicine, Desmodium heterocarpon (L.

Progression in Near-Infrared Fluorescence Imaging Technology for Lung Cancer Management.

Lung cancer is a major threat to human health and a leading cause of death. Accurate localization of tumors in vivo is crucial for subsequent treatment. In recent years, fluorescent imaging technology has become a focal point in tumor diagnosis and treatment due to its high sensitivity, strong selectivity, non-invasiveness, and multifunctionality.

Diffuse Large B-Cell Lymphoma in a Cynomolgus Monkey.

A captive 17-year-old male cynomolgus monkey (Macaca fascicularis) developed diffuse large B-cell lymphoma (DLBCL). This was the first report of DLBCL presenting with a mandible mass and violation of the paranasal sinus in a cynomolgus monkey. The neoplasm showed marked microscopical malignant aspects.

Biodegradation of Crude Oil by Nitrate-Reducing, Sulfate-Reducing, and Methanogenic Microbial Communities under High-Pressure Conditions.

Carbon capture, utilization, and storage (CCUS) is an important component in many national net-zero strategies, and ensuring that CO can be safely and economically stored in geological systems is critical. Recent discoveries have shown that microbial processes (e.g.

Strategically designed metal-free deep-ultraviolet birefringent crystals with superior optical properties.

Finding new birefringent materials with deep-ultraviolet (DUV, < 200 nm) transparency is urgent, as current commercial materials cannot meet the rapidly growing demands in related application fields. Herein, three guanidinium-based compounds, C(NH)CHSO, β-C(NH)Cl, and γ-C(NH)Cl, all featuring [C(NH)·X] (X = CHSO and Cl) pseudo layers, were designed through structural motif tailoring. Theoretical calculations indicate that these metal-free compounds all possess broad bandgaps (6.

Synthesis, evaluation and structure-activity relationship studies of pterodontic acid acylated derivatives with anti-flu A virus (H1N1) activity in vitro.

In order to develop antiviral drugs, we utilized pterodontic acid (Poa-1) as a lead compound and conducted various modifications, including oxidation, reduction, addition, esterification, and acylation, resulting in the synthesis of 29 derivatives, of which 25 were novel acylation derivatives. Cell-level validation demonstrated that 4 derivatives exhibited significant inhibitory effects on the influenza A virus (H1N1), with an IC = 4.04-36.

Machine learning based on metabolomics unveils neutrophil extracellular trap-related metabolic signatures in non-small cell lung cancer patients undergoing chemoimmunotherapy.

Background: Non-small cell lung cancer (NSCLC) is the primary form of lung cancer, and the combination of chemotherapy with immunotherapy offers promising treatment options for patients suffering from this disease. However, the emergence of drug resistance significantly limits the effectiveness of these therapeutic strategies. Consequently, it is imperative to devise methods for accurately detecting and evaluating the efficacy of these treatments.

Clinical significance and biological function of interferon regulatory factor 1 in non-small cell lung cancer.

The clinical application and biological function of interferon regulatory factor 1 (IRF1) in non-small cell lung cancer (NSCLC) patients undergoing chemoimmunotherapy remain elusive. The aim of this study was to investigate the predictive and prognostic significance of IRF1 in NSCLC patients. We employed the cBioPortal database to predict frequency changes in IRF1 and explore its target genes.

LC/MS-guided isolation and GNPS procedures to identify flavonoid of HCoV-OC43 inhibitors.

The screening of based target compounds supported by LC/MS, MS/MS and Global Natural Products Social (GNPS) used to identify the compounds 1-10 of Butea monsperma. They were evaluated in human malignant embryonic rhabdomyoma cells (RD cells) infected with Human coronavirus OC43 (HCoV-OC43) and showed significant inhibitory activity. Target inhibition tests showed that compounds 6 and 8 inhibited the proteolytic enzyme 3CLpro, which is widely present in coronavirus and plays an important role in the replication process, with an effective IC value.

Whole-genome sequencing of a worldwide collection of sugarcane cultivars (Saccharum spp.) reveals the genetic basis of cultivar improvement.

Sugarcane is the main source of sugar worldwide, and 80% of the sucrose production comes from sugarcane. However, the genetic differentiation and basis of agronomic traits remain obscure. Here, we sequenced the whole-genome of 219 elite worldwide sugarcane cultivar accessions.

Diarylheptanoids with neuroprotective effects from Alpinia officinarum rhizomes.

Forty-three diarylheptanoids were isolated from Alpinia officinarum rhizomes among them eight ones (1-6) were undescribed compounds whose structures were identified by UV, IR, HRESIMS, and NMR. The neuroprotective effects of these diarylheptanoids were evaluated on HO-damaged SH-SY5Y cells. Compounds 7, 10, 12, 20, 22, 25, 28, 33, 35, 37, and 42 presented significant neuroprotective effects than that of the positive control (EGCG) at the concentrations of 5, 10 or 20 μM.