A preliminary study on the clinical characteristics of lacrimal duct obstruction in patients with a history of anterior uveitis.

Aim: This study aimed to investigate the clinical features of lacrimal duct obstruction (LDO) in patients with a history of anterior uveitis (AU) and explore preventive strategies by analyzing their demographic, anatomical, and surgical outcome profiles.

Methods: We retrospectively reviewed 40 LDO patients (58 eyes) with a history of AU, treated between 2020 and 2024, comparing them to non-AU LDO controls. Data included demographics, AU-epiphora temporal relationships, obstruction characteristics (location, extent, and adhesions), and surgical outcomes.

Dahuang Huanglian Decoction alleviates dysbiosis by inhibiting GBP5/NLRP3 signaling pathway-mediated pyroptosis of colonic epithelial cells.

Ethnopharmacological Relevance: Currently, the prevalence of dysbiosis is increasing, but its treatment options are limited. Dahuang Huanglian Xiexin Decoction (XXD) is a traditional herbal prescription recorded in the Treatise on Typhoid Fever, with a longstanding application in the treatment of digestive system diseases. It consists mainly of three classical chinese medicinal herbs: Dahuang (Rheum palmatum L.

Identification and validation of biomarkers in Alzheimer's disease based on machine learning algorithms and single-cell sequencing analysis.

Objective: Alzheimer's disease (AD) is a complicated neurodegenerative disease with unknown pathogenesis. Identifying possible diagnostic markers of AD is essential to elucidate its mechanisms and facilitate diagnosis.

Methods: A total of 295 samples (153 AD and 142 normal) were analyzed from two datasets (GSE122063 and GSE132903) in the Gene Express Omnibus (GEO) database.

ESM-Ezy: a deep learning strategy for the mining of novel multicopper oxidases with superior properties.

The UniProt database is a valuable resource for biocatalyst discovery, yet predicting enzymatic functions remains challenging, especially for low-similarity sequences. Identifying superior enzymes with enhanced catalytic properties is even harder. To overcome these challenges, we develop ESM-Ezy, an enzyme mining strategy leveraging the ESM-1b protein language model and similarity calculations in semantic space.

Lingguizhugan decoction enhances autophagy of Alzheimer's disease via regulating the mTOR/ p70s6K pathway and .

Introduction: Lingguizhugan decoction (LGZG) has been reported to treat Alzheimer's disease (AD) by anti-inflammatory and transporting amyloid-β (Aβ).

Methods: Using APP/PS1 transgenic mice as model and gave LGZG decoction by oral gavage. Using Aβ-induced SH-SY5Y cells as model and then added LGZG medicated serum (LMS) to observe the regulatory effect of LGZG on AD autophagy-related pathways.

Licochalcone A Ameliorates Cognitive Dysfunction in an Alzheimer's Disease Model by Inhibiting Endoplasmic Reticulum Stress-Mediated Apoptosis.

BackgroundEndoplasmic reticulum (ER) stress-induced neurodegeneration has been considered an underlying cause of Alzheimer disease (AD). Here, we investigated the beneficial effects of licochalcone A (Lico A), a valuable flavonoid of the root of the Glycyrrhiza species, against cognitive impairment in AD by regulating ER stress.MethodsThe triple transgenic mouse AD models were used and were administrated 5 or 15 mg/kg Lico A.

Protocol to use protein language models predicting and following experimental validation of function-enhancing variants of thymine-N-glycosylase.

Protein language models (PLMs) are machine learning tools trained to predict masked amino acids within protein sequences, offering opportunities to enhance protein function without prior knowledge of their specific roles. Here, we present a protocol for optimizing thymine-DNA-glycosylase (TDG) using PLMs. We describe steps for "zero-shot" enzyme optimization, construction of plasmids, double plasmid transfection, and high-throughput sequencing and data analysis.

Protein language models-assisted optimization of a uracil-N-glycosylase variant enables programmable T-to-G and T-to-C base editing.

Current base editors (BEs) use DNA deaminases, including cytidine deaminase in cytidine BE (CBE) or adenine deaminase in adenine BE (ABE), to facilitate transition nucleotide substitutions. Combining CBE or ABE with glycosylase enzymes can induce limited transversion mutations. Nonetheless, a critical demand remains for BEs capable of generating alternative mutation types, such as T>G corrections.

A Novel Fluorescence Probe Based on Azamonardine for Detecting and Imaging Cysteine in Cells and Zebrafish with High Selectivity and Sensitivity.

A novel fluorescent probe based on azamonardine (Aza) fluorophore was designed and synthesized for the highly selective detection of cysteine (Cys) in vivo and in vitro. After reacting with acryloyl chloride, the fluorescence of Aza is effectively quenched, resulting in the formation of the Aza-acryl probe. Upon the addition of Cys, the ester bond of Aza-acryl is cleaved, releasing a new compound (Compound ) with strong fluorescence, thereby achieving fluorescence turn-on detection of Cys.

Rational design of a novel acryl-modified CQDs fluorescent probe for highly selective detection and imaging of cysteine in vitro and in vivo.

A novel fluorescent nanoprobe CQDs-O-Acryl has been designed and synthesized to directly and accurately identify Cys over other biothiols in PBS (10 mM, pH 7.4) buffer. The carbon quantum dots (CQDs-OH) (λ maxima = 495/525 nm) were fabricated by a solvothermal method using resorcinol as the carbon source.

Sanwu Baisan decoction inhibits colorectal cancer progression in mice by remodeling gut microbiota and tumorigenesis.

Objective: To uncover the anti-tumor effects and potential mechanism of Sanwu Baisan Decoction (, SWB) in treatment of colorectal cancer (CRC) in mice.

Methods: Therapeutic effect was evaluated based on body weight gain, tumor volume, tumor growth inhibition rate, and histological changes and apoptosis in the tumor tissues. Anti-tumor immunity was studied by measuring plasma levels of anti-tumor cytokines, interleukin 6 (IL-6), interleukin 17 (IL-17), and interferon γ (IFN-γ).

Primary lacrimal sac lymphoma: a case-based retrospective study in a Chinese population.

Aim: To determine the clinical characteristics, pathological types, tumor markers, treatments, and outcomes of Chinese patients with primary lacrimal sac lymphoma.

Methods: This case-based retrospective study analyzed 15 Chinese patients with primary lacrimal sac lymphoma. The clinical data collected included gender, age at diagnosis, symptoms, imaging examination results, pathologic diagnosis, pathogen identification, tumor markers, treatments, follow-up, and prognosis.

Baicalin prevents fibrosis of human trabecular meshwork cells via inhibiting the MyD88/NF-κB pathway.

Trabecular meshwork fibrosis contributes to increased aqueous humor outflow resistance, leading to elevated intraocular pressure in primary open-angle glaucoma. Baicalin, an extract from Scutellaria baicalensis Georgi, has shown anti-fibrotic effects in liver, lung, and kidney diseases. However, its anti-fibrotic effect on human trabecular meshwork (HTM) cells has not yet been clarified.

One-step microwave synthesis of red-emissive carbon dots for cell imaging in extreme acidity and light emitting diodes.

Red emissive carbon dots (R-CDs) have received great attention in biological fields due to their deep tissue penetrability, great bioimaging capability, low interference from auto-fluorescence, and potential for optoelectronic applications. Herein, excitation-independent, highly acid-sensitive R-CDs were successfully obtained one-step microwave treatment of -phenylenediamine (-PD) and phosphoric acid and carefully purified by column chromatography. The relationship between the fluorescence emission and surface groups of the R-CDs was studied in detail using XPS, NMR, and fluorescence spectroscopy, and the different mechanisms of action of the R-CDs and acid in HO and ethanol were determined.

A red-emitting Europium(III) complex as a luminescent probe with large Stokes shift for the sequential determination of Cu and biothiols in real samples.

In this work, a novel Eu-DTPA-bis(AMC) complex with red luminescence was designed and synthesized for sequential detection of Cu and biothiols (Cys/Hcy/GSH) based on the displacement strategy with the good selectivity, high sensitivity, and large Stokes shift (288 nm). The possible detection mechanism was verified by UV-vis, the high-resolution mass spectrometry, and the fluorescence decay curve. The experimental parameters, including the solution pH, the incubation time, the concentration ratio of Eu-DTPA-bis(AMC) to Cu and biothiols concentration, were optimized.

Chinese Herbal Medicine Adjusting Brain Microenvironment via Mediating Central Nervous System Lymphatic Drainage in Alzheimer's Disease.

Due to its complex pathogenesis and lack of effective therapeutic methods, Alzheimer's disease (AD) has become a severe public health problem worldwide. Recent studies have discovered the function of central nervous system lymphatic drainage, which provides a new strategy for the treatment of AD. Chinese herbal medicine (CHM) has been considered as a cure for AD for hundreds of years in China, and its effect on scavenging β-amyloid protein in the brain of AD patients has been confirmed.

CdS quantum dots-decorated InOOH: Facile synthesis and excellent photocatalytic activity under visible light.

For the first time, CdS quantum dots (QDs)-decorated InOOH (CdS-In for short) was synthesized by a facile photodeposition method. The experiment results showed that CdS-In samples exhibited excellent activity and stability towards photocatalytic reduction of nitro aromatics. The conversion ratio of 4-nitroaniline (4-NA) over CdS-In sample that was prepared with photodeposition time of 120 min (CdS-In-120) reached up to 99.

Photothermometric analysis of bismuth ions using aggregation-induced nanozyme system with a target-triggered surface cleaning effect.

The development of nanozyme-based photothermometric sensing for point-of-care testing (POCT) heavy metal ions is of great significance for disease diagnosis and health management. Considering the low catalytic activity of most nanozymes at physiological pH, we found bismuth ions (Bi) could effectively enhance the peroxidase (POX)-like activity of cetyltrimethylammonium bromide and citrate-capped octahedral gold nanoparticle (CTAB/Cit-AuNP) nanozymes. It is mainly based on Biions being able to trigger the surface cleaning effect of CTAB/Cit-AuNPs.

[Industrial Emission Characteristics and Control Countermeasures of VOCs in Chinese Rapid Economic Development Areas].

With the rapid development of China's economy, volatile organic compounds (VOCs) as the precursor of smog and ozone are of increasing concern, especially in rapidly developing areas. This paper is a systematic analysis of VOCs emissions and distribution trends in 12 typical industrial sectors, garbage and wastewater treatment plants, comprehensive industrial parks, and residential districts in Beijing-Tianjin-Hebei, Yangtze River Delta, and Pearl River Delta Regions. The results show that pharmacy, rubber producing, as well as paint spraying are the top three industries among the 12 typical industries with the highest average VOCs emission concentrations at 541, 499, and 450 mg·m, respectively.

Berberine hydrochloride inhibits inflammation and fibrosis after canalicular laceration repair in rabbits.

Aims: This study was designed to investigate the molecular mechanisms underlying the anti-inflammatory and anti-fibrosis effects of Berberine hydrochloride (BBR) following canalicular laceration (CL) surgical repair.

Main Methods: We used a rabbit CL model in this study. BBR and the control medicine were administered during and after the surgical operation.

Preliminary report on screening IGSF3 gene mutation in families with congenital absence of lacrimal puncta and canaliculi.

Aim: To investigate the variation of IGSF3 gene in three families with congenital absence of lacrimal puncta and canaliculi, and to lay a foundation for further research on the pathogenic gene of congenital lacrimal duct agenesis.

Methods: The members of the three families were recruited. The ophthalmologic examinations in details, including slit-lamp biomicroscope, intraocular pressure and fundus examination, were carried out.