CDLR-net: a ECG classification network based on deep residual shrinkage networks and LSTM.

Many traditional classification networks directly use the limb two-lead signal (MLII) ECG signals as input for training. However, this method suffers from reduced accuracy when ECG features are not obvious, especially for premature heartbeats. To solve the issue, this paper proposed a novel network, namely CDLR-Net, that combines a Deep Residual Shrinkage Network (DRSN) with a Long Short-Term Memory (LSTM).

Transcriptome Combined with Mendelian Randomization to Identify and Validate Biomarkers Associated with Parthanatos in Sepsis.

Purpose: Sepsis, which is triggered by infection and characterized by a systemic inflammatory response, has been associated with the diagnosis of sepsis, although the detailed molecular basis remains unclear. This study explores parthanatos-related genes (PRGs) as potential biomarkers for sepsis diagnosis and treatment.

Patients And Methods: Data from GSE65682, GSE167363, and GSE95233 were analyzed.

Small bowel metastasis during organ preservation in patients with esophageal squamous cell carcinoma: case report and literature review.

Background: For patients with locally advanced esophageal squamous cell carcinoma (ESCC) who achieve a clinical complete response (cCR) after comprehensive treatment, organ preservation and observation-waiting strategies provide a more conservative treatment option that enhances the patient's quality of life. The high cCR rate of chemoradiotherapy combined with immunotherapy is driving the shift in ESCC treatment from traditional radical surgery to organ preservation.

Case Summary: We report a case of a 57-year-old male patient diagnosed with esophageal ESCC who underwent combined radiotherapy, chemotherapy, and immunotherapy.

Annexin A2: regulating glioma's fate and a potential therapeutic target.

High recurrence and mortality in glioma are linked to aggressive proliferation. Recent strategies focus on genetic interventions, with Annexin A2 (ANXA2) emerging as a promising target. We wanted to evaluate ANXA2's prognostic value and its role in tumor progression, aiming to establish it as a viable therapeutic target.

Single-cell analysis reveals CD34CD90 endothelial cells promote tumor metastasis in gallbladder cancer.

Gallbladder cancer (GBC) is the most common malignancy of the biliary tract, with high metastasis incidence and extremely low survival rate. The tumor endothelial cells (TECs) are fundamental components in the tumor microenvironment and significantly contribute to various tumor progression; however, the roles of TECs in GBC are poorly understood. Here, using single-cell RNA sequencing, we identify a GBC-enriched endothelial population-CD34CD90 ECs (SAEndo2).

Highly selective and portable fluorescent detection of uranyl in groundwater via AIEgen-labeled protein cleavage.

To achieve highly selective and portable detection of uranyl (UO) in potentially polluted groundwater, a fluorescent detection method based on UO-triggered protein cleavage was established. An aggregation-induced emission luminogen (AIEgen), 4-aldehyde-4',4″-bis(4-pyridyl)triphenylamine, (CHO-BIPY-TPA, abbreviated as CBPT), was synthesized and labeled onto bovine serum albumin (BSA). The fluorescence of CBPT-BSA was quenched by UO-triggered covalent bond cleavage and protein fragment release.

Genetic Evidence on the Role of Blood Phytosterols in Frailty: A Mendelian Randomization Study.

Phytosterols have been recommended as a lifestyle intervention for early lipid management-which has a significant impact on frailty. However, their effect on frailty remains unclear. Studies have shown that genetic proxied total blood phytosterol affects the development of cardiovascular disease through non-HDL-c and apolipoprotein B mediation, which makes phytosterol an underlying risk factor for frailty.

Astragaloside IV regulates FOXM1 deubiquitination to ameliorate trophoblast damage caused by high glucose.

Background: Gestational diabetes mellitus (GDM) is a common metabolic complication during pregnancy that poses significant risks to both the pregnant woman and her fetus. Astragaloside IV (Ast IV) belongs to the class of triterpenoid saponins and exhibits important physiological roles in various aspects, including antidiabetic, antioxidant, and antiviral effects. The main objective of this study is to investigate the effects of Ast IV on trophoblast damage caused by high glucose (HG) and its underlying mechanism of action.

Retrospective epidemiological analysis of pulmonary tuberculosis in the older adult and characterization of rifampicin resistance-associated mutations in Nantong City, China (2014-2023).

Objective: To analyze the prevalence trend of older adult pulmonary tuberculosis (ETB) and the distribution and outcome of rifampicin-resistant gene mutation in ETB patients in Nantong.

Methods: The pulmonary tuberculosis patients' data in Nantong from 2014 to 2023 were from Tuberculosis Information Management Syste and ETB and rifampicin-resistant mutation patients were retrospectively analyzed.

Results: From 2014 to 2023, the overall standardized incidence of ETB in Nantong showed a trend of rapid decline and tended to a stable trend stabilized.

Single-cell sequencing and machine learning reveal the role of dioxin-interacting genes in HCC prognosis and immune microenvironment.

Dioxins are persistent environmental pollutants that bioaccumulate in the food chain, posing significant risks to human health. Despite their low environmental concentrations, dioxins accumulate in tissues, particularly in top predators and humans, reaching levels far exceeding environmental exposure. Although the carcinogenic potential of dioxins is well recognized, the molecular mechanisms driving hepatocellular carcinoma (HCC) development remain poorly understood.

Erk1/2 Orchestrates SSPH I-Induced Oxidative Stress, Mitochondrial Dysfunction and Ferroptosis in Hepatocellular Carcinoma.

Although Erk1/2 has been linked to oxidative stress regulation in hepatocellular carcinoma (HCC), the interplay among Erk1/2, reactive oxygen species (ROS), and iron metabolism remains poorly characterised. The steroidal saponin SSPH I, a recognised ferroptosis inducer, exerts dual pharmacological effects via Erk1/2 and ROS-dependent pathways. This study aimed to investigate the regulatory mechanisms of Erk1/2 in ferroptosis and oxidative stress and analyse their feedback regulatory effects on Erk1/2 in HCC using SSPH I as a pharmacological probe, and further elucidate the anti-HCC effects and mechanisms of SSPH I in vitro and in vivo.

The Size Effect on the Phase Transition and Dielectric Properties of Poly(vinylidene Fluoride) Ferroelectric Polymers.

Multi-scale characterization techniques have been employed to analyze the size effect of microstructure on the phase transition behavior and dielectric properties of poly(vinylidene fluoride) (PVDF) films. The results show that oriented amorphous fraction layers are prone to form in the vicinity of the grain boundaries of nano-grained films, while the interfacial polarization and electrostriction effect play a major role. Polar nano-regions are prone to form in micro-grained films, and the maximum fraction of polar crystalline phase and maximal dielectric constant can be achieved due to the balance between the intrinsic effect and extrinsic effect of the material.

Eu-COF-based fluorescence and portable detection of acetone in exhaled breath.

Exhaled biomarker detection, as a non-invasive and portable analytical method, holds great potential in the field of point-of-care testing. Acetone exhibits higher concentrations in the exhaled breath of diabetic patients compared to healthy individuals. Therefore, the development of highly sensitive, easy-to-operate, and portable acetone detection technology is desirable for the rapid screening of diabetes.

Radioresistance in Hepatocellular Carcinoma: Biological Bases and Therapeutic Implications.

Hepatocellular carcinoma (HCC) is a malignant tumor with high morbidity and mortality. Radiotherapy technology is a common treatment modality that can be used in all stages of HCC. However, in some cases, radiotherapy fails in clinical practice mainly because of the patient's resistance to radiotherapy, creating a bottleneck for future breakthroughs.

Collaborative management of severe periodontal-endodontic lesion with intense lip sucking: a case report.

Background: Lip behaviors can lead to maxillofacial developmental disorders such as anterior open bite, posterior crossbite, increased overjet, and a higher risk of developing a Class II malocclusion. However, lip-sucking, a behavior often underreported and overlooked, may adversely affect the periodontal health of permanent teeth in adolescents under extreme conditions if not promptly identified and managed.

Case Presentation: We report a unique case of an 11-year-old boy with a severe periodontal-endodontic lesion on tooth 32, caused by prolonged, high-frequency, and high-intensity lip sucking.

Single-cell landscape of the intrahepatic ecosystem in alcohol-related liver disease.

Alcohol-related liver disease (ALD) is a common chronic liver disease caused by long-term excessive alcohol consumption and responsible for more than half of all liver-related deaths worldwide. The molecular mechanisms associated with ALD were not fully understood. In this study, we performed single-cell RNA sequencing on liver tissues obtained from ALD patients and healthy liver donors.

Spectroscopic study of energy transfer in collisions between vibrational excited H2 and CO2.

The collisional energy transfer between vibrational excited H2(1, 7) and CO2 was investigated by exciting H2 to a vibrational excited state of v = 1, J = 7 by the stimulated Raman scattering technique. The coherent anti-Stokes Raman spectroscopy (CARS) technique determined that H2 was excited to the H2(1, 7) state. Varying the cuvette temperature, the number of H2(1, 7) particles was found to increase with the increase in H2 molar ratio α by scanning the intensity of the CARS spectrum, with peaks at different α at a temperature of 363 ± 15 K, but the peak temperature was not sensitive to α.

Comprehensive Parent-Metabolite PBPK/PD Modeling Insights Into Methotrexate Personalized Dosing Strategies in Patients With Rheumatoid Arthritis.

Rheumatoid arthritis (RA) is a major public health concern, which can cause serious outcomes. Low-dose methotrexate (MTX) is a cornerstone in RA treatment, but there is significant heterogeneity in clinical response. To evaluate underlying sources of pharmacokinetic variability and clinical response of MTX, a physiologically based pharmacokinetic and pharmacodynamic (PBPK/PD) model was developed using PK-sim and Mobi (version 11.

Concomitant use of sodium-glucose co-transporter 2 inhibitors and metformin and the risk of osteomyelitis reporting: a disproportionality analysis based on FAERS database.

Background: Recent clinical case reports have generated controversy concerning the adverse events (AEs) of amputation linked to sodium-glucose co-transporter 2 inhibitors (SGLT2i). We assessed the risk of osteomyelitis AE reporting linked to SGLT2i or SGLT2i-metformin co-medication.

Research Design And Methods: Investigated the FDA Adverse Event Reporting System for osteomyelitis-related AEs associated with SGLT2i or SGLT2i-metformin co-medication from 2013q2 to 2023q1.

Radiofrequency Ablation Therapy versus Stereotactic Body Radiation Therapy for Naive Hepatocellular Carcinoma (≤5cm): A Retrospective Multi-Center Study.

Purpose: Radiofrequency ablation (RFA) is a micro-invasive treatment for early-stage HCC patients. Stereotactic body radiation therapy (SBRT) has also been proven an effective and safe treatment for HCC patients. This multi-center study is to compare the efficacy of computed tomography (CT)-guided RFA and CT-based SBRT in naïve HCC patients with tumor diameters ≤5 cm.

Identification of novel ABCB4 variants and genotype-phenotype correlation in progressive familial intrahepatic cholestasis type 3.

Progressive familial intrahepatic cholestasis type 3 (PFIC3) is a severe hepatic disorder characterized by cholestasis. Elucidating the genotype-phenotype correlations and expanding the mutational spectrum of the ABCB4 gene are crucial for enhancing diagnostic accuracy and therapeutic strategies.Clinical and genetic data from 2 original PFIC3 patients from our institution, along with 118 additional cases identified through a comprehensive literature review, were integrated for a comprehensive analysis.

Helicobacter pylori Infection Is Not Associated with Nonalcoholic Fatty Liver Disease: A Two-Year Cohort Study.

Introduction: Previous studies reported inconsistent results of the association between Helicobacter pylori infection and nonalcoholic fatty liver disease (NAFLD).

Methods: A cohort study of 2,063 adults without NAFLD at baseline, who participated in a repeated health checkup including a 13C-urea breath test and abdominal ultrasonography, was conducted to evaluate the link between H. pylori infection and NAFLD development.

Discovery of as a prognostic biomarker in glioma cells: insights into its cell-cycle relationship and differential expression in non-neuronal cells.

Background: Gliomas, originating from the most common non-neuronal cells in the brain (glial cells), are the most common brain tumors and are associated with high mortality and poor prognosis. Glioma cells exhibit a tendency to disrupt normal cell-cycle regulation, leading to abnormal proliferation and malignant growth. This study investigated the predictive potential of in gliomas and explored its relationship with the cell cycle.

α-Ketoglutarate promotes cardiomyocyte proliferation and heart regeneration after myocardial infarction.

The neonatal mammalian heart can regenerate following injury through cardiomyocyte proliferation but loses this potential by postnatal day 7. Stimulating adult cardiomyocytes to reenter the cell cycle remains unclear. Here we show that cardiomyocyte proliferation depends on its metabolic state.