Experimental Study on the Growth of Methane Hydrate by Amino Acid Solution in the Oil-Water System.

The impact of different surfactants on hydrate formation varies, and exploring hydrate growth characteristics is crucial for advancing the industrial application of oil and gas transportation. This study employed a microscope to investigate the hydrate formation rate and the morphology and formation process of the hydrate along the wall. It also visually demonstrated the hydrate formation process on the wall within an oil-water system and the migration patterns of different liquid phases inside the hydrate, leading to the characteristic of various wall hydrate growth modes.

Case Report: Three-year follow-up of TACE for recurrent undifferentiated pleomorphic sarcoma: sustained local control and no systemic progression.

We report a case of recurrent undifferentiated pleomorphic sarcoma (UPS) in the right calf of a 48-year-old female patient who had undergone initial surgical resection in February 2018. The patient underwent two sessions of transcatheter arterial chemoembolization (TACE) between June and July 2022. During the procedures, superselective catheterization of the tumor-feeding arteries via the posterior tibial artery was performed, followed by infusion of epirubicin and sequential embolization with lipiodol, 300-500 μm microspheres, and gelatin sponges.

The joint role of systemic immune-inflammation index and geriatric nutritional risk index in cancer survivors and their impact on all-cause mortality.

Introduction: This study aimed to investigate the combined predictive value of the Systemic Immune-Inflammation Index (SII) and the Geriatric Nutritional Risk Index (GNRI) for all-cause mortality in cancer survivors.

Methods: Using NHANES data (1999-2018), 2,969 eligible cancer survivors were categorized into four groups based on SII and GNRI levels. Mortality risk was assessed through unadjusted and fully adjusted Cox proportional hazards models.

Esophagectomy enhances hypertension remission and metabolism via weight loss in esophageal Cancer patients with hypertension.

Previous studies have documented hypertension remission following bariatric surgeries and other gastrointestinal surgeries, but the impact of esophagectomy on hypertension remains unclear. This study assesses whether esophagectomy induces hypertension remission in esophageal cancer patients and identifies influencing factors. This retrospective study included 66 esophageal cancer patients with hypertension who underwent esophagectomy (2017-2020).

Global burden and regional disparities of rheumatoid arthritis among the working-age population: A comprehensive analysis from 1990 to 2021 with projections to 2040.

Objective: To evaluate the age-standardized incidence (ASIR), prevalence (ASPR), death (ASDR), and disability-adjusted life year (DALY) rates of rheumatoid arthritis (RA) among the working-age population from 1990 to 2021.

Methods: The data is sourced from the Global Burden of Disease 2021. Estimated annual percentage change (EAPC) was utilized to assess temporal trends.

Evaluation of Low-Coverage Sequencing Strategies for Whole-Genome Imputation in Pacific Abalone .

Low-coverage whole-genome sequencing (lcWGS) followed by imputation is emerging as a cost-effective method for generating a substantial number of single nucleotide polymorphism (SNP) in aquatic species with highly heterozygous and complex genomes. This study represents the first systematic investigation into the application of low-coverage whole-genome sequencing (lcWGS) combined with imputation for genotyping in Pacific abalone () without a reference panel. We utilized 1059 Pacific abalone individuals sequenced at an average depth of 7.

Morphology and molecular phylogeny of two soil species of Holostichides (Ciliophora, Hypotrichia) collected from eastern China, including a new species and a new subspecies.

Newly discovered hypotrich ciliates with evolutionary innovations are challenging to the established systematics and greater interest. Based on living morphology, infraciliature, and SSU rDNA, two bakuellid taxa-including a new species and a new subspecies-collected from subtropical soil environments in eastern China were investigated in the present study. Holostichides (Extraholostichides) eastensis ningboensis n.

Changes in blood pressure and metabolic factors of patients after esophagectomy assessed based on a propensity score matching approach.

Purpose: The aim of this study is to analyze the changes in blood pressure after esophagectomy and the postoperative metabolic factors of esophageal cancer patients with and without combined hypertension.

Methods: Case data of 203 patients with esophageal cancer treated by esophagectomy were retrospectively collected. Patients were divided into 51 cases in the combined hypertension group and 152 cases in the uncomplicated hypertension group according to whether they had preoperative combined hypertension or not.

Berberine restrains non-small cell lung cancer cell growth, invasion and glycolysis via inactivating the SPC25/NUF2 pathway.

Berberine (BBR) has been proved to inhibit the malignant progression of non-small cell lung cancer (NSCLC), but the underlying molecular mechanism still needs to be further revealed. NSCLC cells (A549 and H1299) were treated with BBR. CCK8 assay, colony formation assay, flow cytometry, TUNEL staining and transwell assay were used to examine cell proliferation, apoptosis and invasion.

Exploring the biogeography, morphology, and phylogeny of the condylostomatid ciliates (Alveolata, Ciliophora, Heterotrichea), with establishment of four new species and a revision including redescriptions of five species found in China.

Unlabelled: Species of the ciliate class Heterotrichea Stein, 1859 are a cosmopolitan group of unicellular eukaryotic microorganisms, many of which have been widely used as models in various fields of research such as regenerative biology, functional ecology, environmental toxicology, and symbiotic behavior. However, species identification in the heterotrich family Condylostomatidae, especially the most species-rich and type genus Bory de Saint-Vincent, 1824, remains challenging due to incomplete original descriptions, few reliable distinguishing characters, and overlapping features between different species. This study presents an updated revision of and its related genus da Silva Neto, 1994 based on descriptions of five species, including nine populations collected from China, using both morphological and molecular methods.

CircVIRMA enhances cell malignant behavior by governing the miR-452-5p/CREBRF pathway in cervical cancer.

Our current study aimed to investigate the role and mechanism of circVIRMA in cervical cancer (CC) progression. CircVIRMA, microRNA-452-5p (miR-452-5p) and CREB3 regulatory factor (CREBRF) mRNA levels were examined in CC via quantitative real-time PCR (qRT-PCR). The protein level of CREBRF in CC was checked by Western blot.

Findings on three endocommensal scuticociliates (Protista, Ciliophora) from freshwater mollusks, including their morphology and molecular phylogeny with descriptions of two new species.

Unlabelled: Species of the ciliate genera and are found as endocommensals of terrestrial and freshwater mollusks, respectively. So far, there have been few studies of these genera and morphological data for most members are often incomplete. In the present work, two new species, sp.

Weight loss, glycolipid profile changes in type 2 diabetes patients after esophagectomy: a propensity score matching analysis.

Background: Type 2 diabetes mellitus (T2DM) is a common co-morbidity in patients who receive esophagectomy and has unfavorable effects on glucose and lipid metabolism in patients. This study examines how weight and glycolipid metabolism change in patients with T2DM following esophagectomy.

Methods: This retrospective, one-center, observational analysis with a propensity score matching analysis (PSM) included 114 patients who underwent esophageal surgery in the Department of Cardiothoracic Surgery, the 900th Hospital of Joint Logistic Support Force from 2017 to 2020, which were separated into T2DM group and Non-T2DM group.

Effect of oesophagectomy on lipid profiles in patients with oesophageal cancer combined with hyperlipidaemia: a retrospective study.

Background: Surgery is widely regarded as a pivotal therapeutic approach for treating oesophageal cancer, and clinical observations have revealed that many oesophageal cancer patients also present with concomitant hyperlipidaemia. It is surprising that few studies have been performed to determine how blood lipid levels are affected by oesophageal cancer resection. This research was designed to assess the influence of oesophageal cancer resection on lipid profiles among individuals diagnosed with both oesophageal cancer and hyperlipidaemia.

A self-supervised classification model for endometrial diseases.

Purpose: Ultrasound imaging is the preferred method for the early diagnosis of endometrial diseases because of its non-invasive nature, low cost, and real-time imaging features. However, the accurate evaluation of ultrasound images relies heavily on the experience of radiologist. Therefore, a stable and objective computer-aided diagnostic model is crucial to assist radiologists in diagnosing endometrial lesions.

Taxonomic and Phylogenetic Studies of Two Brackish Species (Protista, Ciliophora, Scuticociliatia) from Subtropical Coastal Waters of China, with Report of a New Species.

The genus Dujardin, 1841, with nearly 40 morphospecies, is one of the largest genera in the well-known subclass Scuticociliatia. In the present study, two species were collected from subtropical coastal waters of the East China Sea. The morphology and molecular phylogeny were investigated using modern standard methods.

Integration of Non-Coding RNA and mRNA Profiles Reveals the Mechanisms of Rumen Development Induced by Different Types of Diet in Calves.

Selecting suitable feed types and understanding the gastrointestinal digestive mechanism are helpful for the growth and health of calves in intensive dairy farming. However, the effects on rumen development of changing the molecular genetic basis and the regulatory mechanism by using different feed types are still unclear. Nine 7-day-old Holstein bull calves were randomly divided into GF (concentrate), GFF (alfalfa: oat grass = 3:2) and TMR (concentrate: alfalfa grass: oat grass: water = 0.

The widely reported but poorly studied ciliate family Folliculinidae (Protozoa, Ciliophora, Heterotrichea): a revision with notes on its taxonomy, morphology and phylogenetic relationships.

Unlabelled: Ciliates of the heterotrich family Folliculinidae are widespread in various habitats and are distinguished by their transparent loricae of various shapes, conspicuous peristomial lobes, and dimorphic life cycles. They usually attach firmly to the surface of substrates, feed on bacteria and microalgae, and play a significant role in energy flow and material cycling in the microbial food web. However, little is known regarding their biodiversity and systematics.

Paramecium bursaria as a Potential Tool for Evaluation of Microplastics Toxicity.

Microplastics (MPs) are normally defined as small plastic wastes with a size of 1 μm to 5 mm in diameter. This tiny plastic debris is abundant in aquatic systems and poses a great threat to aquatic biota. To date, toxicological assessment of MPs is predominantly dependent on metazoan animals, although their applications are sometimes limited due to the high cost, narrow ecological niche, or ethical considerations.

How to promote grass roots medical treatment under China's graded diagnosis and treatment policy? - From the perspective of customer value theory.

Objectives: Based on the background of the implementation of graded diagnosis and treatment policy (GDT) in China, this paper studied the service optimization of primary medical institutions from the perspective of the primary medical treatment rate.

Methods: First, the difference-in-difference (DID) empirical strategy is used to analyze the impact of the implementation of the GDT on the improvement of the primary medical attendance rate, and the parallel trend test and the placebo test were used to test the robustness to illustrate the necessity of policy implementation. Second, combined with customer value theory, this paper used a logistic regression model to study the impact of customer value perception on grassroots medical care.

A Case Study of the Morphological and Molecular Variation within a Ciliate Genus: Taxonomic Descriptions of Three Species (Ciliophora, Cyrtophoria), with the Establishment of a New Species.

Three species, Claparède & Lachmann, 1859; Faria et al., 1922; and n. sp.

New Data Define the Molecular Phylogeny and Taxonomy of Four Freshwater Suctorian Ciliates With Redefinition of Two Families Heliophryidae and Cyclophryidae (Ciliophora, Phyllopharyngea, Suctoria).

Four suctorian ciliates, Gönnert, 1935, (Kormos & Kormos, 1958) Dovgal, 2002, (Hentschel, 1916) Matthes, 1954 and Ehrenberg, 1838, were collected from a freshwater lake in Ningbo, China. The morphological redescription and molecular phylogenetic analyses of these ciliates were investigated. Phylogenetic analyses inferred from SSU rDNA sequences show that all three suctorian orders, Endogenida, Evaginogenida, and Exogenida, are monophyletic and that the latter two clusters as sister clades.

Morphology, Life Cycle, and SSU rDNA-based Phylogeny of Two Folliculinid Ciliates (Ciliophora, Heterotrichea, Folliculinidae) Collected from Subtropical Coastal Wetlands of China.

Most ciliates of the heterotrich family Folliculinidae are flagship periphytic species. They are characterized by their transparent lorica, conspicuous peristomial lobes, and dimorphic life cycle. However, the understanding of their biodiversity and systematics is relatively poor.

Taxonomy and SSU rDNA-Based Phylogeny of Two Heterotrich Ciliates (Ciliophora, Heterotrichea) Collected From Subtropical Wetlands of China, Including the Description of a New Species, n. sp.

The Heterotrichea Stein, 1859 are a group of ciliated protists (single-celled eukaryotes) that occur in a wide variety of aquatic habitat where they play important roles in the flow of nutrients and energy within the microbial food web. Many species are model organisms for research in cytology and regenerative biology. In the present study, the morphology and phylogeny of two heterotrich ciliates, namely, n.

miR-9-5p Mediates ABCC1 to Elevate the Sensitivity of Glioma Cells to Temozolomide.

Chemotherapy combined with surgery is an important clinical treatment for glioma, but endogenous or acquired temozolomide (TMZ) resistance can lead to poor prognosis. microRNA (miR)-9-5p acts in biological function of glioma, but the drug resistance of miR-9-5p in glioma is under exploration. The study intended to test the molecular mechanism of miR-9-5p in glioma cells.