Translational fidelity and longevity are genetically linked.

Aging is a series of adverse changes over time that increases mortality risk. Several hypotheses have been proposed to explain aging, including Leslie Orgel's Error-Catastrophe Theory, which asserts that translation errors erode the translational machinery, become self-amplifying, and eventually lead to death. Evidence for the theory is scarce, especially regarding intra-specific fidelity-longevity correlations.

Role of ferroptosis-related IREB2 in the shared genetic etiology between smoking and facial aging: Insights from large-scale genome-wide cross-trait analysis.

While the association between smoking and accelerated facial aging is well documented, the specific pathways underlying this association remain poorly understood. To investigate the shared genetic architecture between smoking and facial aging, we performed genetic analyses based on genome-wide association studies (GWAS) data. These analyses included linkage disequilibrium score regression (LDSC), pleiotropic analysis under composite null hypothesis (PLACO), functional mapping and annotation (FUMA), and multi-marker analysis of genomic annotation (MAGMA).

Improved calibration and 3D reconstruction for micro fringe projection profilometry.

Micro fringe projection profilometry (MFPP) is a crucial three-dimensional measurement technology widely used in industrial inspection. As the system measurement accuracy is limited by calibration and reconstruction errors, this paper proposes a joint calibration of telecentric camera and projector, and develops noise-robust 3D reconstruction, ultimately forming a high-precision MFPP for microscopic object measurement. Specifically, the optimal parameters for the telecentric camera model are determined by constructing a virtual 3D calibration target, which circumvents the limitations that plane-based calibration methods often produce local optimal solutions.

Development and selection of stably expressed reference genes for expression normalization in Ribes odoratum under drought stress.

The stability of internal reference genes is of great significance for the study of gene functions, and the development and screening of stable internal reference genes is the key to gene function analysis. Real-time reverse transcription quantitative PCR (qPCR) is an important tool to measure gene expression levels. Selection of stable reference genes for data normalization is a prerequisite.

C-Arg9-APCR3-VHL promotes β-catenin degradation via the VHL-mediated ubiquitin-proteasome system in APC-mutant colorectal cancer.

Mutations in the adenomatous polyposis coli (APC) gene are common in colorectal cancer (CRC) and result in deregulation of β-catenin, a key driver of tumor initiation and progression. Despite its central role, targeted degradation of β-catenin remains an unmet therapeutic need in APC-mutant CRC. This study introduces and evaluates C-Arg9-APCR3-VHL, a novel PROTAC designed to promote β-catenin degradation via the VHL-Mediated ubiquitin-proteasome pathway.

Knowledge, attitudes, and practices regarding body weight management among patients with overweight or obesity: a cross-sectional study.

Objectives: This study aimed to investigate the knowledge, attitudes, and practices (KAP) related to weight management among overweight or obese patients.

Methods: A cross-sectional study was conducted in May 2024 at the First Hospital of Zhangjiakou, focusing on overweight or obese patients. Self-administered questionnaires were used to collect demographic data and assess participants' KAP scores.

Facilitating Anodic Ammonia Oxidation over Trace Cobalt-Substituted Solid Solution of Platinum to Boost Direct Ammonia Fuel Cell up to 853.75 mW cm.

Low-temperature direct ammonia fuel cell (DAFC) is a key technology for decarbonized electric generation on a large scale and with high safety. However, as the core of DAFC, the anodic ammonia oxidation reaction (AOR) still suffers from unsatisfactory activity and an ambiguous reaction mechanism. Herein, inspired by homogeneously catalyzed oxidation of ammonia, a nucleophilic attack mechanism that loosens the steric and charge constraints in heterogeneous catalysis is theoretically predicted and experimentally verified by a three-stage isotope-labeling experiment.

Altitude regulates the response of nitrogen removal function to short-term flooding events in a riparian ecotone.

Understanding how historical flooding duration and contemporary extreme flooding events interact to affect the ecosystem processes is necessary to develop biochemical models and accurately predict ecological functions in the future. In this study, we focused on the nitrogen (N) removal processes of riparian ecosystems (i.e.

Insight into the shared pathogenic link between COVID-19 and pterygium: a systematic bioinformatics analysis with experimental validation.

The outbreak of coronavirus disease 2019 (COVID-19) has resulted in a remarkable threat to global public health over the past few years. Despite the tremendous studies of COVID-19 ongoing, few have focused on the viral impact on the ocular surface. As one of the most common inflammatory diseases of the ocular surface, pterygium could be triggered under multiple environmental exposures.

Deciphering mitochondrial dysfunction in keratoconus: Insights into ACSL4 from machine learning-based bulk and single-cell transcriptome analyses and experimental validation.

Keratoconus (KC) is a prevalent ectatic corneal disease and the leading cause of corneal transplantation globally. Despite evidence of mitochondrial abnormalities in KC, the underlying mechanisms remain unclear. Our aim was to investigate the role of mitochondrial dysfunction in this pathological condition.

Astragalus Injection Modulates the Pharmacokinetics of Doxorubicin and CYP450 Enzymes.

Background: Doxorubicin (DOX) is a widely used anthracycline antibiotic for the treatment of breast cancer, liver cancer, lymphoma, and other malignant tumors. However, its clinical application is limited by the side effects and drug resistance. Astragalus injection has been combined with DOX in the treatment of cancer, which improves the curative effect and reduces drug resistance.

KRT6A Restricts Influenza A Virus Replication by Inhibiting the Nuclear Import and Assembly of Viral Ribonucleoprotein Complex.

The transcription and replication of the genome of influenza A virus (IAV) take place in the nucleus of infected cells, which is catalyzed by the viral ribonucleoprotein (vRNP) complex. The nuclear import of the vRNP complex and its component proteins is essential for the efficient replication of IAV and is therefore prone to be targeted by host restriction factors. Herein, we found that host cellular protein keratin 6A (KRT6A) is a negative regulator of IAV replication because siRNA-mediated knockdown of expression increased the growth titers of IAV, whereas exogenous overexpression of KRT6A reduced viral yields.

RNA Editing-Mediated Correction of TP53 Nonsense Mutations via Lipid Nanoparticle-Delivered Circular ADAR-Recruiting RNAs.

Nonsense mutations account for over 20% of disease-associated mutations, which refer to the occurrence of premature termination codons (PTCs) in gene sequences, resulting in truncated and dysfunctional proteins. Nonetheless, due to poor accessibility of precise target sites and the limitations of gene editing tools, there is still a lack of safe, effective, and site-specific approach for correction of nonsense mutations. Here, we designed a circular ADAR-recruiting RNA (Circ-arRNA) for the in vivo RNA editing-mediated repair of the nonsense mutation.

Predicting estrogen receptor agonists from plastic additives across various aquatic-related species using machine learning and AlphaFold2.

The absence of effective public databases greatly limits high-throughput prediction of hormonal effects mediated by nuclear receptors in aquatic organisms. In this study, we developed novel strategies for multi-species screening of estrogen receptor (ER) agonists in plastic additives using AlphaFold2. Firstly, Deep Forest (DF), artificial neural network (ANN) and conventional machine learning (ML) models were utilized to screen ERα agonists.

A cluster-scale CuFe heteroatom ensemble with a multisite interface reinforces electronic interaction to boost ambient ammonia electrosynthesis.

Herein, we report a cluster-scale CuFe heteroatom ensemble for electrochemical nitrate reduction to ammonia. The multisite interface reinforces electronic interaction with adsorbates, thus achieving a high NH yield rate of 10.51 mmol h mg and Faradaic efficiency of 93.

New Psychoactive Substance Esketamine Causes Endocrine-Disrupting Effects and Developmental Toxicity.

Esketamine (ESK), a new psychoactive substance known for its strong hallucinogenic effect, has been detected in surface water worldwide. The toxicity of ESK to fish at a certain environmental concentration remains unclear. In this study, zebrafish embryos and ZF4 cells were exposed to ESK (0, 0.

Comprehensive Immune Landscape in Allergic Conjunctivitis: Insights from Mendelian Randomization Analysis.

Introduction: Current understanding of the immune landscape underlying allergic conjunctivitis (AC) remains rather limited. We investigated the potential association between circulating immunophenotypes and AC using Mendelian randomization (MR) methodology.

Methods: Based on genome-wide association study (GWAS) summary-level statistics, a 2-sample MR was employed to analyze the bidirectional causal relationships between 731 circulating immunophenotypes and AC risk.

Novel H16N3 avian influenza viruses isolated from migratory gulls in China in 2023.

As a rare subtype of avian influenza virus, H16 viruses are predominant in gulls but rarely found in domestic birds. The low prevalence of H16 viruses has limited our understanding of their epidemiology and evolutionary dynamics. In this study, we isolated three novel H16N3 viruses from migratory gulls in East Asian-Australasian Flyway in eastern China in 2023, which are significantly different from previously identified isolates.

Benzimidazole Fungicide Carbendazim Induces Gut Inflammation through the TLR5/NF-κB Pathway in Grass Carp.

Fungicides have been increasingly used across various sectors, including agriculture and textiles. The biocidal properties of fungicides may negatively impact the stability of intestinal microbiota, thereby posing a threat to intestinal health. In this study, we investigated the mechanisms of intestinal damage and functional abnormalities in grass carp following a 42-day exposure to the widely used fungicide carbendazim at environmentally relevant concentrations (0.

Gender-specific effects of dydrogesterone on zebrafish liver metabolism after long-term exposure.

Synthetic progestin dydrogesterone is widely used in gynecology and animal husbandry, leading to high environmental detection rates and concentrations. Dydrogesterone influences sex differentiation, gonad development, and spawning in fish. However, its impact on the liver, a vital organ for hormone production and detoxification, remains largely unknown.

Antitussive drug dextromethorphan induces developmental impairment in zebrafish.

Dextromethorphan (DXM) is a common ingredient in cough and cold remedies. Despite its widespread presence in aquatic environments, the impact of DXM on fish remains largely unknown. This study evaluated the developmental impairment of zebrafish embryos exposed to DXM from 2 hours post-fertilization (hpf) to 14 days post-fertilization (dpf) at five different exposure concentrations: 0.

Evaluating pulmonary toxicity of PFOS and its alternative OBS using spheroids of A549 cells.

Sodium p-perfluorous nonenoxybenzene sulfonate (OBS) is a prominent alternative to perfluorooctanesulfonic acid (PFOS). Numerous studies have demonstrated hepatotoxicity and neurotoxicity of OBS and PFOS in mammals. The lungs, as a sensitive organ, are among the potential target organs for OBS and PFOS exposure.

Multiomics analysis reveal the impact of 17α-Ethinylestradiol on mortality in juvenile zebrafish.

17α-Ethinylestradiol (EE2) is known for its endocrine-disrupting effects on embryonic and adult fish. However, its impact on juvenile zebrafish has not been well established. In this study, juvenile zebrafish were exposed to EE2 at concentrations of 5 ng/L (low dose, L), 10 ng/L (medium dose, M), and 50 ng/L (high dose, H) from 21 days post-fertilization (dpf) to 49 dpf.

Development and evaluation of suitable reference genes for qRT-PCR normalization of hybrids derived from Lycium barbarum and Lycium ruthenicum.

Background: A correct and stably expressing reference gene is prerequisite for successful quantitative real-time PCR (qRT-PCR). Investigating gene expression profiling during flower development could enhance our understanding of the molecular mechanisms of flower formation and fertility in Lycium.

Methods And Results: In this study, 11 candidate reference genes in Lycium flower development were selected from transcriptome sequence data and evaluated with five traditional housekeeping genes from previous studies based on qRT-PCR amplification.