Cross-lineage 5-methylcytosine methylome profiling reveals methylated divergence among Toxoplasma gondii tachyzoites of the three major clonal lineages.

Background: Toxoplasma gondii is a globally widespread zoonotic parasite, infecting nearly one-third of the human population, often leading to chronic, latent infections. Among the emerging layers of gene regulation, 5-methylcytosine (mC) has emerged as a pivotal post-transcriptional modification in eukaryotes. Despite its growing recognition in various species, the epitranscriptomic landscape of mC in the tachyzoite stage of T.

and activity of quisinostat against .

an opportunistic pathogen, poses severe threats to immunocompromised individuals and fetuses of newly infected pregnant women. The current gold-standard treatment, a combination of pyrimethamine and sulfadiazine, is limited by severe adverse events, necessitating the development of novel therapeutic agents. , a CCK8 assay demonstrated that quisinostat inhibited HeLa cell proliferation in a dose-dependent manner, with a CC of 8.

Identification of circulating inflammation cytokines as a mediator of gut microbiota and type 2 diabetes mellitus: a Mendelian randomization study.

Background: Several studies have suggested that the gut microbiota (GM) may be associated with type 2 diabetes mellitus (T2DM). However, the causal relationship between GM and T2DM and whether inflammatory cytokines act as mediators remain unclear.

Aims: To investigate the association between GM and T2DM and the proportion of this association that is mediated through inflammatory cytokines.

Diffusion MRI-based analysis of functional alterations of the glymphatic system in children with non-lesional epilepsy.

Objective: Based on diffusion tensor imaging (DTI) data, we used DTI-analysis along the perivascular space (DTI-ALPS) and free-water mapping to investigate the function of the glymphatic system and its relationship with clinical features among pediatric patients with magnetic resonance imaging (MRI)-negative non-lesional epilepsy (NLE).

Methods: A total of 83 NLE children (mean age 9.25 ± 4.

Molecular Prevalence and Genotyping of in Sheep Tissues Intended for Human Consumption in Shanxi Province, North China.

is one of the most widely distributed intracellular parasites worldwide, which can infect humans and a wide range of warm-blooded animals including sheep, with felines serving as its definitive host. infection in sheep can lead to premature births, abortions and stillbirths, causing significant economic losses to the sheep industry. Sheep farming has become a key pillar of the agricultural economy in Shanxi Province, North China, but little is known about infection in sheep in this province.

Lvvibriocin-GK effectively reduced skin ulcer syndrome of Apostichopus japonicus by eliminating surface bacteria, modulating gut microbiota, and enhancing host immune responses.

Skin ulceration syndrome (SUS) is a major threat to the aquaculture of Apostichopus japonicus, particularly in southern China, where it has shown high mortality rates and infectious potential. Traditional antibiotic treatments often lead to challenges such as antibiotic resistance. Antimicrobial peptides (AMPs), which are vital elements of innate immunity, represent a promising alternative for treating SUS.

Anti-Toxoplasma gondii efficacy of beta, beta-dimethylacrylshikonin and isobutyrylshikonin in vitro and in vivo.

Background: Toxoplasma gondii is a widespread parasite that can infect almost all vertebrate species including humans, causing variable clinical symptoms from asymptomatic infection to serious diseases. Though extensive research has been done in recent decades, the prevention and control of T. gondii continue to present substantial challenges.

Glucose Parameters, Inflammation Markers, and Gut Microbiota Changes of Gut Microbiome-Targeted Therapies in Type 2 Diabetes Mellitus: a Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Purpose: This meta-analysis aims to summarize the effects of gut microbiome-targeted therapies (MTTs) on glucometabolic, inflammatory factors and gut microbiota in patients with type 2 diabetes mellitus (T2DM).

Methods: 4 databases were searched for randomized controlled trials (RCTs) that included subjects with T2DM who received MTTs. All results were presented as standardized mean difference (SMD)/MD and 95% confidence intervals (95% CIs).

Spatiotemporal Diffusion, Colonization, and Antibody Responses in Susceptible C57BL/6J Mice Orally Infected with Cysts.

is an obligate intracellular protozoan that infects humans and other mammals. The C57BL/6J mouse strain is regarded as an ideal model organism for studying due to its susceptibility to infection and its other advantages over other laboratory animals. However, systematic studies on the response dynamics of the susceptible C57BL/6J mice after oral infection with cysts are lacking.

High-contrast electrochromic nano fiber films changing from yellow-green to black based on butterfly monomer for smart windows and self-adaptive camouflage.

High contrast electrochromic (EC) materials have attracted much attention due to their great potential for applications in fields such as electronic displays, smart windows (thermal management) and adaptive camouflage. However, obtaining EC materials with high contrast and long term stability is still a challenge. Herein, we designed and synthesized a butterfly (large planar core-distorted periphery) diamine monomer (DCTPA-NH) by fusing two triphenylamine (TPA) units on edge of 7H-dibenzocarbazolyl, and prepared four polyamides (PAs) through polycondensation of the monomer with different diacids, which exhibit excellent solution processability, thermal stability, electrochemical redox reversibility.

The effect of GLP-1 receptor agonists on circulating inflammatory markers in type 2 diabetes patients: A systematic review and meta-analysis.

Aim: To investigate whether the antidiabetic agent glucagon-like peptide-1 receptor agonists (GLP-1 RAs) can exert anti-inflammatory effects while lowering blood glucose, we performed a meta-analysis and systematic review.

Methods: We searched 4 online databases (Medline, Embase, Cochrane Library and the Web of Science) for randomised controlled trials (RCTs) that examined changes after GLP-1RAs intervention in commonly accepted biomarkers of inflammation: C-reactive protein (CRP), tumour necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), leptin, adiponectin, plasminogen activator inhibitor-1 (PAI-1), monocyte chemotactic protein-1(MCP-1) and advanced glycation end products (AGEs).

Results: This meta-analysis included 52 eligible RCTs (n = 4734) with a median follow-up of 24 weeks, a mean age of 54.

The effect of tanshinones on cognitive impairments in animal models of Alzheimer's disease: a systematic review and meta-analysis.

Background: Alzheimer's disease (AD) is an age-related neurological illness that poses a significant hazard to human health. A fat-soluble compound called tanshinones was isolated from Danshen, a traditional Chinese herb. Recent years have seen reports of clinical trials examining the effects of tanshinones on cognitive impairment among individuals with AD, as well as the publication of pertinent basic research.

Heteroatom Introduction and Electrochemical Reconstruction on Heterostructured Co-Based Electrocatalysts for Hydrogenation of Quinolines.

Electrocatalytic hydrogenation (ECH) of quinoline provides an eco-friendly and prospective route to achieve the highly value-added generation of 1,2,3,4-tetrahydroquinoline (THQ). Co element has been proven to be the efficient catalytic site for ECH of quinoline, but the rational regulation of the electronic structure of active Co site to improve the activity is still a challenge. Herein, the hierarchical core-shell structure consisting of NiCo-MOF nanosheets encapsulated Cu(OH) nanorods (Cu(OH)@CoNi-MOF) is constructed.

Application of Diffusional Kurtosis Imaging on Normal-Appearing White Matter in Cerebral Small Vessel Disease.

Background: This study aimed to investigate the diagnostic potential of diffusional kurtosis imaging (DKI) parameters in detecting pathological alterations in the normal-appearing white matter (NAWM) associated with cerebral small vessel disease (CSVD).

Methods: A total of 56 patients diagnosed with CSVD were enrolled, all exhibiting confirmed lacunar infarction in the corticospinal tract (CST) as verified by conventional magnetic resonance imaging. A control group of 24 healthy individuals who exhibited no discernible abnormalities on conventional magnetic resonance imaging (MRI) scans was also included.

First Molecular Identification of spp. in Sheep, Beef Cattle, and Dairy Cattle in Shanxi Province, North China.

spp. are common zoonotic intestinal protozoa, which can lead to serious intestinal diseases in both humans and animals through fecal-oral transmission, leading to significant economic losses and public health challenges. To reveal the prevalence of in sheep and cattle in Shanxi Province, North China, fecal samples were collected from 311 sheep, 392 dairy cattle, and 393 beef cattle from three representative counties in the northern, central, and southern regions of Shanxi Province.

Elevation of ANXA1 associated with potential protective mechanism against ferroptosis and immune cell infiltration in age-related macular degeneration.

Background: Age-related macular degeneration (AMD), is a neurodegenerative ocular disease. This study investigated the role of ferroptosis-related genes and their interaction with immune cell infiltration in AMD.

Methods: We screened differential expression genes (DEGs) of AMD from data sets in Gene Expression Omnibus.

Exploring the impact of APOE ɛ4 on functional connectivity in Alzheimer's disease across cognitive impairment levels.

The apolipoprotein E (APOE) ɛ4 allele is a recognized genetic risk factor for Alzheimer's Disease (AD). Studies have shown that APOE ɛ4 mediates the modulation of intrinsic functional brain networks in cognitively normal individuals and significantly disrupts the whole-brain topological structure in AD patients. However, how APOE ɛ4 regulates brain functional connectivity (FC) and consequently affects the levels of cognitive impairment in AD patients remains unknown.

Dietary patterns and diabetic microvascular complications risk: a Mendelian randomization study of European ancestry.

Purpose: Previous observational studies about the link between dietary factors and diabetic microvascular complications (DMCs) is controversial. Thus, we systemically assessed the potential causal relationship between diet and DMCs risk using Mendelian randomization (MR) methods.

Methods: We used genome-wide association studies (GWAS) statistics to estimate the causal effects of 17 dietary patterns on three common DMCs in European.

UPLC-Q-TOF/MS-based urine metabolomics for the diagnosis and staging of bladder cancer.

Background: Bladder cancer (BC) is a common malignant tumour of the urinary system. Currently, the gold standard for diagnosing BC is cystoscopy, but it is an invasive examination that can lead to a certain psychological burden on the patient. In this study, we aimed to identify non-invasive potential metabolic biomarkers that could improve the diagnostic accuracy of bladder cancer.

Proteomics analysis reveals the differential protein expression of female and male adult Toxocara canis using Orbitrap Astral analyzer.

Background: Toxocara canis, the most prevalent helminth in dogs and other canines, is one of the socioeconomically important zoonotic parasites, particularly affecting pediatric and adolescent populations in impoverished communities. However, limited information is available regarding the proteomes of female and male adult T. canis.

APOE ε4-associated downregulation of the IL-7/IL-7R pathway in effector memory T cells: Implications for Alzheimer's disease.

Introduction: The apolipoprotein E (APOE) ε4 allele exerts a significant influence on peripheral inflammation and neuroinflammation, yet the underlying mechanisms remain elusive.

Methods: The present study enrolled 54 patients diagnosed with late-onset Alzheimer's disease (AD; including 28 APOE ε4 carriers and 26 non-carriers). Plasma inflammatory cytokine concentration was assessed, alongside bulk RNA sequencing (RNA-seq) and single-cell RNA sequencing (scRNA-seq) analysis of peripheral blood mononuclear cells (PBMCs).

A 14-amino acid cationic peptide Bolespleenin from the marine fish mudskipper Boleophthalmus pectinirostris exhibiting potent antimicrobial activity and therapeutic potential.

Antimicrobial peptides (AMPs) are an important component of innate immunity in both vertebrates and invertebrates, and some of the unique characteristics of AMPs are usually associated with their living environment. The marine fish, mudskipper Boleophthalmus pectinirostris, usually live amphibiously in intertidal environments that are quite different from other fish species, which would be an exceptional source of new AMPs. In the study, an AMP named Bolespleenin was identified, which was a truncated peptide derived from a new functional gene found in B.